From Grain to Plate: Quality Attributes and Economic Indicators of Sorghum Rice from Selected Cultivars

1Doctoral Program in Agriculture Industry Technology, Faculty of Agroindustrial Technology and Biosystems, Universitas Brawijaya, Malang, East Java 65145, Indonesia.
2Department of Food Crops and Horticulture, State Agricultural Polytechnic of Kupang, Lasiana, Kupang, East Nusa Tenggara 85111, Indonesia.
3Agriculture Industry Technology, Faculty of Agroindustrial Technology and Biosystems, Universitas Brawijaya, Malang, East Java 65145, Indonesia.
4Department of Food Science and Biotechnology, Faculty of Agroindustrial Technology and Biosystems, Universitas Brawijaya, Malang, East Java 65145, Indonesia.
5Center of Excellence on Tubers and Roots, Faculty of Agroindustrial Technology and Biosystems, Universitas Brawijaya, Malang, East Java 65145, Indonesia.
6Department of Food Technology, University of Muhammadiyah Malang, Malang, East Java 65144, Indonesia.
7Department of Public Health, Nusa Cendana University, Kupang, East Nusa Tenggara 85111, Indonesia.
8Department of Nutrition Science, Faculty of Medicine, Universitas Brawijaya, Indonesia.

Background: Sorghum (Sorghum bicolor L.) is an alternative cereal with strong potential for food security in semi-arid regions. In Indonesia’s East Nusa Tenggara Province, several local cultivars distinguished by seed colour are cultivated. This study evaluates their physical, bioactive content and economic feasibility to identify the best candidates for commercial production.

Methods: Cultivars differentiated by seed colour were analysed for physical attributes (firmness, colour and cooking time), bioactive compounds (phytate, tannin, total phenols and antioxidant activity) and economic feasibility via production cost analysis.

Result: Significant variations were observed among cultivars. Texture firmness ranged from 56.83 to 834.57 g, with red sorghum being the softest and white sorghum the hardest. Cooking time varied from 19 to 105 minutes, with white-red sorghum being the fastest. Phytic acid content ranged from 0.013 to 0.076%, tannin from 0.0174 to 0.1323 mg/g and total phenols from 90.68 to 139.03 ppm, with dark-coloured cultivars showing the highest bioactive content. Production costs ranged from IDR 10,909-20,658/kg, with white-red sorghum showing optimal efficiency (ratio 0.092). White-red and yellow sorghum offer the best balance of sensory acceptability and economic viability for the mainstream market, while black and red sorghum suit the premium functional food segment, supporting regional food security.

Global food security faces multidimensional challenges from climate change, population growth and pressures on agricultural resources. Rice (Oryza sativa L.), as a primary staple, is ecologically vulnerable due to its high water demands. Water scarcity is projected to affect 15-20 million hectares of irrigated rice land by 2025, while global food production must rise by 50%, requiring 30% more water. This necessitates developing alternative cereals that are both nutritionally adequate and water efficient.
       
Sorghum (Sorghum bicolor L.) offers high drought tolerance, adaptation to marginal lands and a nutritional profile superior to paddy rice. Its deep root system and physiological mechanism enable survival in water-deficient conditions, making it a strategic option for addressing climate change. Sorghum is high in protein (9-13%), dietary fiber (6%) and essential minerals such as phosphorus (16%) and magnesium (0.1%) (Tanwar et al., 2023), as well as iron and zinc (0.002%), supporting its role in combating malnutrition. Coloured sorghum contains higher essential nutrients, dietary fiber and essential amino acids than white rice (Pontieri et al., 2021). It also contains bioactive compounds such as polyphenols, tannins and anthocyanins with high antioxidant activity and health benefits, including anti-inflammatory, anticancer and glucose-metabolism-regulating effects (Borah et al., 2024).
       
In Indonesia, the government has projected sorghum as a strategic commodity for national food security through local resources food diversification (Badan Pangan Nasional, 2025). Sorghum planting was targeted at 15,000 hectares in 2022 and is planned to expand to 154,000 hectares in 2024. East Nusa Tenggara (NTT), particularly Waingapu Regency, has been designated as a primary development centre with 3,447 hectares, the largest area in Indonesia, with planned expansion to 25,000 hectares in 2023.
       
In the NTT, at least 36 local sorghum cultivars have been identified across Timor, Sumba and Flores, with variation in 17 morphological characteristics, including seed colour, panicle size, plant height and ripening period (Mukkun et al., 2018). This genetic diversity is a valuable resource for developing leading varieties suited to local preferences and agroecological conditions. NTT people traditionally consume sorghum as an alternative staple in the forms of rice, porridge and traditional cakes, providing a sociocultural basis supporting sorghum products (Winarti et al., 2020).
       
However, studies that simultaneously evaluate the quality attributes and economic indicators of NTT local cultivars remain limited. Most prior research has focused on agronomic and morphological aspects. Such information is essential for selecting superior cultivars and recommending the most profitable varieties to farmers and industry.
       
Economic feasibility analysis is essential for stakeholders to support production scalability, since raw materials, energy, water, labor and production efficiency are key determinants in investment and business decisions (Widodo et al., 2023) for sorghum products. Without reliable economic data, sorghum promotion will face adoption barriers due to uncertainties in profitability and price competitiveness with conventional rice.
       
This study addresses this gap through a comprehensive evaluation of NTT sorghum cultivars, combining physical quality attributes, bioactive and anti-nutrient analysis and economic feasibility to identify the most efficient cultivars.
       
The findings are expected to strengthen the sorghum value chain in Indonesia and inform industry development and farmer empowerment policies.
Materials
 
The primary materials were seven sorghum cultivars from East Nusa Tenggara, classified by grain colour: yellow, cream, white-red, black, red, gray and white. This research was conducted at the Laboratory of Food Processing Technology and Biomass, Faculty of Agricultural Technology, Brawijaya University, Malang, Indonesia and the Laboratory of Food Quality Control, State Agricultural Polytechnic of Kupang, East Nusa Tenggara, Indonesia, from August to October 2025. Grains were harvested at optimal ripeness and stored dry. All chemicals for proximate and bioactive analysis were obtained from Merck (pro-analysis grade).
 
Sample preparation
 
Grains were sorted, washed under running water, then soaked for 12 hours at room temperature to accelerate hydration and reduce cooking time. After draining, the grains were cooked into sorghum rice (Fig 1).

Fig 1: Appearance of cooked sorghum rice from NTT provincial cultivars based on grain colour and size.


 
Cooking of sorghum rice
 
Rice was cooked using a Miyako rice cooker (0.6 L) with grain to water ratios specific to each cultivar: yellow, gray (1:3); cream, white-red, white (1:4); black, red (1:10). Cooking time was recorded from cooker activation until full cooking, indicated by soft texture and the cooker switching to warming mode. Cooked rice was cooled to room temperature before further analysis.
 
Analysis of physical profile
 
Texture (hardness) was measured using a texture analyzer fitted with a 35 mm cylinder probe via double compressions to 50% of sample height at a pre-test speed of 2 mm/s; values were averaged over three replicates and expressed in grams (g). Colour was determined with a colorimeter (Konica Minolta CR-400, Japan) in the CIE L*a*b* space averaging five points per sample for L* (lightness), a* (red-green) and b* (yellow-blue), with Chroma [(C* = √(a* + b*)] and Hue angle [(H° = arctan (b*/a*)] (Rao et al., 2022).
 
Analysis of anti-nutrients and bioactive compounds
 
Analysis of phytate content used the colorimetric method with a Wade reagent that has been modified. Total phenol content was measured using the Folin-Ciocalteu method and expressed as ppm of gallic acid equivalents. Antioxidant activity was measured using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method and stated in inhibition percentage.
 
Economic analysis
 
Analysis of economic feasibility was conducted by calculating all the components of the production cost per kilogram of sorghum rice, covering: raw materials (cost of sorghum grains), water (used volume of water × water rate), electricity (power consumption of rice cooker × cooking time × electricity rate), labor (preparation and cooking time × labor fee per hour), cost of overhead (10% of the total of direct costs).
       
The cost-effectiveness ratio was calculated by comparing the total production cost with the resulting rice yield. An analysis of break-even point and profit margin was also conducted to evaluate the feasibility of commercial production.
 
Statistical analysis
 
All analyses were conducted in triplicate (n=3). Data were analyzed by ANOVA, followed by Duncan’s Multiple Range Test (DMRT) at p<0.05 when significant differences were found, using SPSS v25.0. Pearson correlation analysis was performed to evaluate relationships between bioactive parameters. Results are presented as mean ± standard deviation.
The physical characteristics, anti-nutrient and bioactive compound contents of sorghum rice from various cultivars of different colours are presented in Table 1.

Table 1: Physical characteristics, anti-nutrient and bioactive compounds of sorghum rice from various cultivars.


 
Sorghum rice textural profile
 
Texture analysis revealed that sorghum grain hardness ranged from 56.83 g (red) to 834.57 g (white), representing a nearly 15-fold difference across cultivars. White and black sorghum were the hardest (834.57±2.77 g and 407.00±8.19 g), while red sorghum was the softest (56.83±15.81 g). Yellow sorghum (244.67±10.50 g) and white-red sorghum (160.67±14.74 g) occupied intermediate positions closer to the texture range of conventional rice.
       
This variability reflects differences in starch composition, endosperm architecture and polyphenol-protein interactions. High amylose content produces harder grains through strong hydrogen bonding, whereas high amylopectin yields softer, stickier textures (Li et al., 2016). Endosperm structure further modulates this effect: a soft endosperm absorbs water more readily than a corneous endosperm, resulting in faster gelatinisation and softer cooked texture (Yang et al., 2024). In dark pigmented cultivars, condensed tannins may also reinforce hardness by forming tannin-protein complexes (Pontieri et al., 2021).
       
Very hard cultivars such as white sorghum may be less preferred than rice in the typical 200-400 g range, whereas yellow and white-red sorghum are more likely to be accepted. For harder cultivars, prolonged soaking, pressure cooking, or pre-gelatinisation can soften the grain and improve palatability (Yang et al., 2024).
 
Sorghum rice colour profile
 
The colour of cooked sorghum rice varied widely across cultivars. L* values ranged from 15.82 to 60.94, with black sorghum showing the highest lightness, followed by white-red, while gray sorghum was the darkest. The a* parameter ranged from -0.74 to 18.70, peaking in yellow sorghum and slightly negative in white-red sorghum, whereas b* ranged from 9.74 to 21.24 and was highest in white sorghum. Chroma values (10.05-25.29) were greatest in yellow and lowest in white-red sorghum, while hue angles (37.23°- 94.22°) placed white-red in the yellow-green region and gray sorghum closest to pure red (Pontieri et al., 2021). Such wide cultivar-dependent variation in colour attributes, particularly in L*, a* and b* parameters, is consistent with findings reported for other small cereals; Pawase et al., (2019) demonstrated significant differences in colour properties among finger millet and pearl millet cultivars, reflecting genotype-dependent differences in pigment composition and distribution across the grain layers.”
       
No direct correlation was observed between L* and phenolic or tannin content. Black sorghum, despite having the highest L* (60.94), contained high phenol (132.524 ppm) and tannin (0.01323%). Conversely, yellow and gray sorghum, both with low L*, had lower phenol levels (111.748 and 126.796 ppm) than red sorghum, which combined the highest phenol content (139.029 ppm) with only a moderate L* (23.66). This pattern indicates that cooked rice colour is shaped by multiple interacting factors rather than bioactive concentration alone.
 
Sorghum rice cooking time
 
Cooking time ranged from 19±0.5 min (white-red sorghum) to 105±2.0 min (black sorghum), an 86-min span with significant implications for energy use, practicality and commercial economics. Black sorghum required almost five times the time of white-red sorghum and over three times that of conventional rice (25-30 min). Cream sorghum (99±1.5 min) was also very long, while yellow sorghum (73±1.0 min) showed a moderate cooking time. Gray (63±1.2 min) and red sorghum (50±1.0 min) showed comparable cooking times with a difference of only 13 minutes.
       
This variation is primarily affected by grain physical structure, particularly endosperm hardness (Khalid et al., 2022), the thickness of the pericarp (Gwala et al., 2020) and starch composition (Li et al., 2016). A compact corneous endosperm and thick pericarp slow water and heat penetration, delaying starch gelatinisation. Black sorghum’s long cooking time (105 min) likely reflects a very compact endosperm and thick pericarp, creating a mass energy transfer barrier.
       
Interestingly, no direct correlation existed between rice hardness and cooking time. White sorghum (834.57 g hardness) required only 61 min, less than the black sorghum (105 min, 407.00 g hardness).  Likewise, red sorghum (softest at 56.83 g) needed 50 min versus 19 min for white-red sorghum (160.67 g). This suggests cooking time depends on raw grain structure and hydration rate, while cooked-rice texture depends on starch composition and retrogradation.
 
Anti-nutrient compound content
 
Phytate content ranged from 0.013±0.001% to 0.076±0.004%, varying significantly (p<0.05). Red sorghum had the highest level, nearly six times that of the lowest cultivar, indicating high genetic variability in phytic acid accumulation. Black sorghum (0.039±0.002%) and gray sorghum (0.025±0.002%) showed intermediate levels.
       
Yellow, cream, white-red and white sorghum had the lowest phytate content. The 0.013-0.076% range in cooked rice is far lower than that of raw sorghum grains (typically 0.5-1.5% dry weight) (Keyata et al., 2021). This >90% reduction reflects the leaching of phytic acid into the soaking and cooking water and endogenous phytase activity, which hydrolyzes phytic acid into less-phosphorylated inositol phosphate with weaker chelating capacity.
       
Soaking at room temperature provides optimal conditions for endogenous phytase (pH 5.0-5.5), with 30-50% phytic acid hydrolysis achievable during prolonged soaking (Asiri, 2025). Cooking diffuses dissolved phytic acid into the water but inactivates phytase, halting further hydrolysis. This aligns with (Davana et al., 2021), who reported that processing of sorghum, including germination and soaking, markedly reduced phytate and tannin content, supporting the >90% reduction observed in the present study.
       
Tannin content ranged from 0.0174±0.0010 to 0.1323±0.0080 mg/g. Black and red sorghum had the highest content, followed by yellow and gray sorghum, while cream, white and white-red sorghum had the lowest.
       
Tannin content correlated with grain colour intensity: black and red sorghum had the highest tannins, while white sorghum had the lowest and the brightest cooked colour (L* = 57.22). The correlation is imperfect, however, as yellow and gray sorghum (very low cooked L*) had only moderate tannins, indicating that pigmentation depends not only on tannins but also on other phenolics, such as anthocyanins and phenolic acid, distributed differently among cultivars (Pontieri et al., 2021).
 
Total phenol content and antioxidant activity
 
Total phenol content ranged from 90.68±1.80 ppm (white-red) to 139.03±3.50 ppm (red and black), with intermediate values for gray, yellow, white and cream sorghum. A strong positive correlation between total phenol and tannin content was observed (r=0.87, p<0.05), consistent with condensed tannins being a subset of phenolic compounds (Pontieri et al., 2021); sorghum phenolics comprise phenolic acids, flavonoids, tannins and stilbenes (de Morais Cardoso et al., 2017). These levels far exceed those of conventional white rice (<50 ppm), though they remain below pigmented red rice (13,000-40,000 ppm) (Wattanavanitchakorn et al., 2025); even so, sorghum, including bright cultivars such as white-red, which offers a clear bioactive advantage over the white rice that dominates Indonesian diets, provides a strong basis for functional-food positioning.
       
Antioxidant activity correlated strongly with total phenol content (r=0.92, p<0.05), with minor deviation attributable to differences among phenolic classes and their synergistic interactions (de Morais Cardoso et al., 2017). Red and black sorghum, with the highest phenol content, showed the highest DPPH scavenging capacity (82.41±1.60% and 79.86±1.50% respectively), while white-red sorghum reached 58.17±1.00%, still markedly above conventional white rice (<30%) (Ranjkesh et al., 2021), a meaningful contribution from a staple food.
 
Analysis of economic feasibility for sorghum rice production
 
Economic feasibility analysis covered production costs per kg of sorghum rice, including raw materials, cultivar-specific water consumption, electricity (cooking-time dependent), labour and overhead (Table 2).

Table 2: Analysis of economic feasibility for sorghum rice production per kilogram.


 
Structure of production cost and price components
 
Total production cost per kg ranged from IDR 10,909 (white-red sorghum) to IDR 20,658 (black sorghum), an 89.4% variation, nearly twofold, with corresponding contribution margins ranging from 27.3% (white-red at IDR 15,000/kg) to 34.1% (red at IDR 25,000/kg), underscoring the critical importance of cultivar selection and market positioning for economic optimisation.
       
Raw material was the largest cost component (73.3% for white-red Sorghum, 48.4% for black sorghum). For raw material costs of IDR 8,000/kg, operational costs ranged from IDR 2,909-9,010; for IDR 10,000/kg cultivars, from IDR 6,464-10,658.
       
Electricity was the largest and most variable operational cost (6.3% for white-red to 18.3% for black sorghum), scaling directly with cooking time: black sorghum (105 min) incurred IDR 3,780 against only IDR 684 for white-red sorghum (19 min), a 5.5-fold difference.
       
Water costs (4.1-7.3% of total) scaled with the cultivar-specific ratios, being highest for black and red sorghum (1:10) owing to greater absorption and evaporation during prolonged cooking. Labor (preparation plus cooking supervision at IDR 2,000/hour) and overhead (10% of direct costs) followed the same pattern, both lowest for white-red and highest for black sorghum (Table 2); at a commercial scale, partial automation and fixed-cost distribution would reduce both proportions substantially.
 
Ratio of cost efficiency and economic ranking of cultivars
 
The cost-effectiveness ratio (1/total cost × 1000) served as a quantitative indicator, with higher values denoting greater economic efficiency. Duncan’s test produced four statistically distinct groups. White-red sorghum achieved the highest ratio (0.0917), 89.4% more efficient than black sorghum, owing to its short cooking time and optimal water use. Gray (0.0709) and yellow sorghum (0.0673) formed the second, with moderate raw-material costs and 61-63 min cooking times. Red (0.0607) and cream sorghum (0.0588) clustered next; red’s higher raw material cost (IDR 10,000 vs. IDR 8,000/kg) was offset by its shorter cooking time (50 vs. 99 min). Black sorghum ranked lowest (0.0484), reflecting the combined penalties of high raw-material price, prolonged cooking and high water consumption.
               
Compared with retail rice (medium IDR 10,000-12,000/kg; premium IDR 15,000-20,000/kg), white-red sorghum (production cost of IDR 10,909/kg) can be priced competitively at IDR 15,000/kg, yielding a contribution margin of IDR 4,091/kg (27,3%) and a monthly profit of approximately IDR 8.2 milion at a production capacity of 100 kg/day. Break-even is reached at 489 kg/month (approximately 5 production days), with a payback period of approximately 6 months, assuming an initial investment of IDR 50 million, confirming its viability as a mainstream healthy alternative grain. Red sorghum, with a production cost of 16.464/kg and a selling price of IDR 25,000/kg, offers the highest contribution margin among all cultivars (34.1%, IDR 8,536/kg), with a payback period of approximately 3 months, making it the most economically attractive for the premium functional food segment. Black sorghum at IDR 25,000/kg yields a margin of 17.4% (IDR 4,342/kg) with a payback period of approximately 6 months, remaining commercially viable for health-conscious consumers. Cream sorghum, however, requires a minimum selling price of IDR 18,900/kg to recover production costs, making it less competitive at the standard market price of IDR 15,000/kg and necessitating premium positioning or further processing efficiency to improve its economic viability.
Comprehensive evaluation of seven sorghum cultivars from East Nusa Tenggara revealed significant variability in physical quality, bioactive and anti-nutrient compounds and economic feasibility, with dark coloured cultivars showing higher bioactives but also higher anti-nutrients. White-red sorghum showed the highest economic efficiency (ratio 0.092) owing to the fastest cooking time and optimal resource use. White-red and yellow sorghum are prospective candidates for mainstream commercial production, balancing sensory acceptability, nutritional value and economic feasibility, while black and red sorghum suit the premium functional-food segment despite higher costs. These findings provide an empirical basis for cultivar selection and commercialisation strategies supporting sustainable food security in Indonesia.
The authors gratefully acknowledge the financial assistance provided by the Impactful Consortium Research Grant, Ministry of Higher Education, Science and Technology.
 
Disclaimers
 
The findings and interpretation are the author’s responsibility and do not necessarily reflect the views of their affiliated institutions.
 
Informed consent
 
This study did not involve human or animal subjects: data were obtained solely from sorghum seeds collected across East Nusa Tenggara Province, Indonesia. As the analysis covered only physical characteristics, bioactive compounds and economic viability, no ethical approval was required.
The authors declare no financial, professional, or personal conflicts of interest.

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From Grain to Plate: Quality Attributes and Economic Indicators of Sorghum Rice from Selected Cultivars

1Doctoral Program in Agriculture Industry Technology, Faculty of Agroindustrial Technology and Biosystems, Universitas Brawijaya, Malang, East Java 65145, Indonesia.
2Department of Food Crops and Horticulture, State Agricultural Polytechnic of Kupang, Lasiana, Kupang, East Nusa Tenggara 85111, Indonesia.
3Agriculture Industry Technology, Faculty of Agroindustrial Technology and Biosystems, Universitas Brawijaya, Malang, East Java 65145, Indonesia.
4Department of Food Science and Biotechnology, Faculty of Agroindustrial Technology and Biosystems, Universitas Brawijaya, Malang, East Java 65145, Indonesia.
5Center of Excellence on Tubers and Roots, Faculty of Agroindustrial Technology and Biosystems, Universitas Brawijaya, Malang, East Java 65145, Indonesia.
6Department of Food Technology, University of Muhammadiyah Malang, Malang, East Java 65144, Indonesia.
7Department of Public Health, Nusa Cendana University, Kupang, East Nusa Tenggara 85111, Indonesia.
8Department of Nutrition Science, Faculty of Medicine, Universitas Brawijaya, Indonesia.

Background: Sorghum (Sorghum bicolor L.) is an alternative cereal with strong potential for food security in semi-arid regions. In Indonesia’s East Nusa Tenggara Province, several local cultivars distinguished by seed colour are cultivated. This study evaluates their physical, bioactive content and economic feasibility to identify the best candidates for commercial production.

Methods: Cultivars differentiated by seed colour were analysed for physical attributes (firmness, colour and cooking time), bioactive compounds (phytate, tannin, total phenols and antioxidant activity) and economic feasibility via production cost analysis.

Result: Significant variations were observed among cultivars. Texture firmness ranged from 56.83 to 834.57 g, with red sorghum being the softest and white sorghum the hardest. Cooking time varied from 19 to 105 minutes, with white-red sorghum being the fastest. Phytic acid content ranged from 0.013 to 0.076%, tannin from 0.0174 to 0.1323 mg/g and total phenols from 90.68 to 139.03 ppm, with dark-coloured cultivars showing the highest bioactive content. Production costs ranged from IDR 10,909-20,658/kg, with white-red sorghum showing optimal efficiency (ratio 0.092). White-red and yellow sorghum offer the best balance of sensory acceptability and economic viability for the mainstream market, while black and red sorghum suit the premium functional food segment, supporting regional food security.

Global food security faces multidimensional challenges from climate change, population growth and pressures on agricultural resources. Rice (Oryza sativa L.), as a primary staple, is ecologically vulnerable due to its high water demands. Water scarcity is projected to affect 15-20 million hectares of irrigated rice land by 2025, while global food production must rise by 50%, requiring 30% more water. This necessitates developing alternative cereals that are both nutritionally adequate and water efficient.
       
Sorghum (Sorghum bicolor L.) offers high drought tolerance, adaptation to marginal lands and a nutritional profile superior to paddy rice. Its deep root system and physiological mechanism enable survival in water-deficient conditions, making it a strategic option for addressing climate change. Sorghum is high in protein (9-13%), dietary fiber (6%) and essential minerals such as phosphorus (16%) and magnesium (0.1%) (Tanwar et al., 2023), as well as iron and zinc (0.002%), supporting its role in combating malnutrition. Coloured sorghum contains higher essential nutrients, dietary fiber and essential amino acids than white rice (Pontieri et al., 2021). It also contains bioactive compounds such as polyphenols, tannins and anthocyanins with high antioxidant activity and health benefits, including anti-inflammatory, anticancer and glucose-metabolism-regulating effects (Borah et al., 2024).
       
In Indonesia, the government has projected sorghum as a strategic commodity for national food security through local resources food diversification (Badan Pangan Nasional, 2025). Sorghum planting was targeted at 15,000 hectares in 2022 and is planned to expand to 154,000 hectares in 2024. East Nusa Tenggara (NTT), particularly Waingapu Regency, has been designated as a primary development centre with 3,447 hectares, the largest area in Indonesia, with planned expansion to 25,000 hectares in 2023.
       
In the NTT, at least 36 local sorghum cultivars have been identified across Timor, Sumba and Flores, with variation in 17 morphological characteristics, including seed colour, panicle size, plant height and ripening period (Mukkun et al., 2018). This genetic diversity is a valuable resource for developing leading varieties suited to local preferences and agroecological conditions. NTT people traditionally consume sorghum as an alternative staple in the forms of rice, porridge and traditional cakes, providing a sociocultural basis supporting sorghum products (Winarti et al., 2020).
       
However, studies that simultaneously evaluate the quality attributes and economic indicators of NTT local cultivars remain limited. Most prior research has focused on agronomic and morphological aspects. Such information is essential for selecting superior cultivars and recommending the most profitable varieties to farmers and industry.
       
Economic feasibility analysis is essential for stakeholders to support production scalability, since raw materials, energy, water, labor and production efficiency are key determinants in investment and business decisions (Widodo et al., 2023) for sorghum products. Without reliable economic data, sorghum promotion will face adoption barriers due to uncertainties in profitability and price competitiveness with conventional rice.
       
This study addresses this gap through a comprehensive evaluation of NTT sorghum cultivars, combining physical quality attributes, bioactive and anti-nutrient analysis and economic feasibility to identify the most efficient cultivars.
       
The findings are expected to strengthen the sorghum value chain in Indonesia and inform industry development and farmer empowerment policies.
Materials
 
The primary materials were seven sorghum cultivars from East Nusa Tenggara, classified by grain colour: yellow, cream, white-red, black, red, gray and white. This research was conducted at the Laboratory of Food Processing Technology and Biomass, Faculty of Agricultural Technology, Brawijaya University, Malang, Indonesia and the Laboratory of Food Quality Control, State Agricultural Polytechnic of Kupang, East Nusa Tenggara, Indonesia, from August to October 2025. Grains were harvested at optimal ripeness and stored dry. All chemicals for proximate and bioactive analysis were obtained from Merck (pro-analysis grade).
 
Sample preparation
 
Grains were sorted, washed under running water, then soaked for 12 hours at room temperature to accelerate hydration and reduce cooking time. After draining, the grains were cooked into sorghum rice (Fig 1).

Fig 1: Appearance of cooked sorghum rice from NTT provincial cultivars based on grain colour and size.


 
Cooking of sorghum rice
 
Rice was cooked using a Miyako rice cooker (0.6 L) with grain to water ratios specific to each cultivar: yellow, gray (1:3); cream, white-red, white (1:4); black, red (1:10). Cooking time was recorded from cooker activation until full cooking, indicated by soft texture and the cooker switching to warming mode. Cooked rice was cooled to room temperature before further analysis.
 
Analysis of physical profile
 
Texture (hardness) was measured using a texture analyzer fitted with a 35 mm cylinder probe via double compressions to 50% of sample height at a pre-test speed of 2 mm/s; values were averaged over three replicates and expressed in grams (g). Colour was determined with a colorimeter (Konica Minolta CR-400, Japan) in the CIE L*a*b* space averaging five points per sample for L* (lightness), a* (red-green) and b* (yellow-blue), with Chroma [(C* = √(a* + b*)] and Hue angle [(H° = arctan (b*/a*)] (Rao et al., 2022).
 
Analysis of anti-nutrients and bioactive compounds
 
Analysis of phytate content used the colorimetric method with a Wade reagent that has been modified. Total phenol content was measured using the Folin-Ciocalteu method and expressed as ppm of gallic acid equivalents. Antioxidant activity was measured using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method and stated in inhibition percentage.
 
Economic analysis
 
Analysis of economic feasibility was conducted by calculating all the components of the production cost per kilogram of sorghum rice, covering: raw materials (cost of sorghum grains), water (used volume of water × water rate), electricity (power consumption of rice cooker × cooking time × electricity rate), labor (preparation and cooking time × labor fee per hour), cost of overhead (10% of the total of direct costs).
       
The cost-effectiveness ratio was calculated by comparing the total production cost with the resulting rice yield. An analysis of break-even point and profit margin was also conducted to evaluate the feasibility of commercial production.
 
Statistical analysis
 
All analyses were conducted in triplicate (n=3). Data were analyzed by ANOVA, followed by Duncan’s Multiple Range Test (DMRT) at p<0.05 when significant differences were found, using SPSS v25.0. Pearson correlation analysis was performed to evaluate relationships between bioactive parameters. Results are presented as mean ± standard deviation.
The physical characteristics, anti-nutrient and bioactive compound contents of sorghum rice from various cultivars of different colours are presented in Table 1.

Table 1: Physical characteristics, anti-nutrient and bioactive compounds of sorghum rice from various cultivars.


 
Sorghum rice textural profile
 
Texture analysis revealed that sorghum grain hardness ranged from 56.83 g (red) to 834.57 g (white), representing a nearly 15-fold difference across cultivars. White and black sorghum were the hardest (834.57±2.77 g and 407.00±8.19 g), while red sorghum was the softest (56.83±15.81 g). Yellow sorghum (244.67±10.50 g) and white-red sorghum (160.67±14.74 g) occupied intermediate positions closer to the texture range of conventional rice.
       
This variability reflects differences in starch composition, endosperm architecture and polyphenol-protein interactions. High amylose content produces harder grains through strong hydrogen bonding, whereas high amylopectin yields softer, stickier textures (Li et al., 2016). Endosperm structure further modulates this effect: a soft endosperm absorbs water more readily than a corneous endosperm, resulting in faster gelatinisation and softer cooked texture (Yang et al., 2024). In dark pigmented cultivars, condensed tannins may also reinforce hardness by forming tannin-protein complexes (Pontieri et al., 2021).
       
Very hard cultivars such as white sorghum may be less preferred than rice in the typical 200-400 g range, whereas yellow and white-red sorghum are more likely to be accepted. For harder cultivars, prolonged soaking, pressure cooking, or pre-gelatinisation can soften the grain and improve palatability (Yang et al., 2024).
 
Sorghum rice colour profile
 
The colour of cooked sorghum rice varied widely across cultivars. L* values ranged from 15.82 to 60.94, with black sorghum showing the highest lightness, followed by white-red, while gray sorghum was the darkest. The a* parameter ranged from -0.74 to 18.70, peaking in yellow sorghum and slightly negative in white-red sorghum, whereas b* ranged from 9.74 to 21.24 and was highest in white sorghum. Chroma values (10.05-25.29) were greatest in yellow and lowest in white-red sorghum, while hue angles (37.23°- 94.22°) placed white-red in the yellow-green region and gray sorghum closest to pure red (Pontieri et al., 2021). Such wide cultivar-dependent variation in colour attributes, particularly in L*, a* and b* parameters, is consistent with findings reported for other small cereals; Pawase et al., (2019) demonstrated significant differences in colour properties among finger millet and pearl millet cultivars, reflecting genotype-dependent differences in pigment composition and distribution across the grain layers.”
       
No direct correlation was observed between L* and phenolic or tannin content. Black sorghum, despite having the highest L* (60.94), contained high phenol (132.524 ppm) and tannin (0.01323%). Conversely, yellow and gray sorghum, both with low L*, had lower phenol levels (111.748 and 126.796 ppm) than red sorghum, which combined the highest phenol content (139.029 ppm) with only a moderate L* (23.66). This pattern indicates that cooked rice colour is shaped by multiple interacting factors rather than bioactive concentration alone.
 
Sorghum rice cooking time
 
Cooking time ranged from 19±0.5 min (white-red sorghum) to 105±2.0 min (black sorghum), an 86-min span with significant implications for energy use, practicality and commercial economics. Black sorghum required almost five times the time of white-red sorghum and over three times that of conventional rice (25-30 min). Cream sorghum (99±1.5 min) was also very long, while yellow sorghum (73±1.0 min) showed a moderate cooking time. Gray (63±1.2 min) and red sorghum (50±1.0 min) showed comparable cooking times with a difference of only 13 minutes.
       
This variation is primarily affected by grain physical structure, particularly endosperm hardness (Khalid et al., 2022), the thickness of the pericarp (Gwala et al., 2020) and starch composition (Li et al., 2016). A compact corneous endosperm and thick pericarp slow water and heat penetration, delaying starch gelatinisation. Black sorghum’s long cooking time (105 min) likely reflects a very compact endosperm and thick pericarp, creating a mass energy transfer barrier.
       
Interestingly, no direct correlation existed between rice hardness and cooking time. White sorghum (834.57 g hardness) required only 61 min, less than the black sorghum (105 min, 407.00 g hardness).  Likewise, red sorghum (softest at 56.83 g) needed 50 min versus 19 min for white-red sorghum (160.67 g). This suggests cooking time depends on raw grain structure and hydration rate, while cooked-rice texture depends on starch composition and retrogradation.
 
Anti-nutrient compound content
 
Phytate content ranged from 0.013±0.001% to 0.076±0.004%, varying significantly (p<0.05). Red sorghum had the highest level, nearly six times that of the lowest cultivar, indicating high genetic variability in phytic acid accumulation. Black sorghum (0.039±0.002%) and gray sorghum (0.025±0.002%) showed intermediate levels.
       
Yellow, cream, white-red and white sorghum had the lowest phytate content. The 0.013-0.076% range in cooked rice is far lower than that of raw sorghum grains (typically 0.5-1.5% dry weight) (Keyata et al., 2021). This >90% reduction reflects the leaching of phytic acid into the soaking and cooking water and endogenous phytase activity, which hydrolyzes phytic acid into less-phosphorylated inositol phosphate with weaker chelating capacity.
       
Soaking at room temperature provides optimal conditions for endogenous phytase (pH 5.0-5.5), with 30-50% phytic acid hydrolysis achievable during prolonged soaking (Asiri, 2025). Cooking diffuses dissolved phytic acid into the water but inactivates phytase, halting further hydrolysis. This aligns with (Davana et al., 2021), who reported that processing of sorghum, including germination and soaking, markedly reduced phytate and tannin content, supporting the >90% reduction observed in the present study.
       
Tannin content ranged from 0.0174±0.0010 to 0.1323±0.0080 mg/g. Black and red sorghum had the highest content, followed by yellow and gray sorghum, while cream, white and white-red sorghum had the lowest.
       
Tannin content correlated with grain colour intensity: black and red sorghum had the highest tannins, while white sorghum had the lowest and the brightest cooked colour (L* = 57.22). The correlation is imperfect, however, as yellow and gray sorghum (very low cooked L*) had only moderate tannins, indicating that pigmentation depends not only on tannins but also on other phenolics, such as anthocyanins and phenolic acid, distributed differently among cultivars (Pontieri et al., 2021).
 
Total phenol content and antioxidant activity
 
Total phenol content ranged from 90.68±1.80 ppm (white-red) to 139.03±3.50 ppm (red and black), with intermediate values for gray, yellow, white and cream sorghum. A strong positive correlation between total phenol and tannin content was observed (r=0.87, p<0.05), consistent with condensed tannins being a subset of phenolic compounds (Pontieri et al., 2021); sorghum phenolics comprise phenolic acids, flavonoids, tannins and stilbenes (de Morais Cardoso et al., 2017). These levels far exceed those of conventional white rice (<50 ppm), though they remain below pigmented red rice (13,000-40,000 ppm) (Wattanavanitchakorn et al., 2025); even so, sorghum, including bright cultivars such as white-red, which offers a clear bioactive advantage over the white rice that dominates Indonesian diets, provides a strong basis for functional-food positioning.
       
Antioxidant activity correlated strongly with total phenol content (r=0.92, p<0.05), with minor deviation attributable to differences among phenolic classes and their synergistic interactions (de Morais Cardoso et al., 2017). Red and black sorghum, with the highest phenol content, showed the highest DPPH scavenging capacity (82.41±1.60% and 79.86±1.50% respectively), while white-red sorghum reached 58.17±1.00%, still markedly above conventional white rice (<30%) (Ranjkesh et al., 2021), a meaningful contribution from a staple food.
 
Analysis of economic feasibility for sorghum rice production
 
Economic feasibility analysis covered production costs per kg of sorghum rice, including raw materials, cultivar-specific water consumption, electricity (cooking-time dependent), labour and overhead (Table 2).

Table 2: Analysis of economic feasibility for sorghum rice production per kilogram.


 
Structure of production cost and price components
 
Total production cost per kg ranged from IDR 10,909 (white-red sorghum) to IDR 20,658 (black sorghum), an 89.4% variation, nearly twofold, with corresponding contribution margins ranging from 27.3% (white-red at IDR 15,000/kg) to 34.1% (red at IDR 25,000/kg), underscoring the critical importance of cultivar selection and market positioning for economic optimisation.
       
Raw material was the largest cost component (73.3% for white-red Sorghum, 48.4% for black sorghum). For raw material costs of IDR 8,000/kg, operational costs ranged from IDR 2,909-9,010; for IDR 10,000/kg cultivars, from IDR 6,464-10,658.
       
Electricity was the largest and most variable operational cost (6.3% for white-red to 18.3% for black sorghum), scaling directly with cooking time: black sorghum (105 min) incurred IDR 3,780 against only IDR 684 for white-red sorghum (19 min), a 5.5-fold difference.
       
Water costs (4.1-7.3% of total) scaled with the cultivar-specific ratios, being highest for black and red sorghum (1:10) owing to greater absorption and evaporation during prolonged cooking. Labor (preparation plus cooking supervision at IDR 2,000/hour) and overhead (10% of direct costs) followed the same pattern, both lowest for white-red and highest for black sorghum (Table 2); at a commercial scale, partial automation and fixed-cost distribution would reduce both proportions substantially.
 
Ratio of cost efficiency and economic ranking of cultivars
 
The cost-effectiveness ratio (1/total cost × 1000) served as a quantitative indicator, with higher values denoting greater economic efficiency. Duncan’s test produced four statistically distinct groups. White-red sorghum achieved the highest ratio (0.0917), 89.4% more efficient than black sorghum, owing to its short cooking time and optimal water use. Gray (0.0709) and yellow sorghum (0.0673) formed the second, with moderate raw-material costs and 61-63 min cooking times. Red (0.0607) and cream sorghum (0.0588) clustered next; red’s higher raw material cost (IDR 10,000 vs. IDR 8,000/kg) was offset by its shorter cooking time (50 vs. 99 min). Black sorghum ranked lowest (0.0484), reflecting the combined penalties of high raw-material price, prolonged cooking and high water consumption.
               
Compared with retail rice (medium IDR 10,000-12,000/kg; premium IDR 15,000-20,000/kg), white-red sorghum (production cost of IDR 10,909/kg) can be priced competitively at IDR 15,000/kg, yielding a contribution margin of IDR 4,091/kg (27,3%) and a monthly profit of approximately IDR 8.2 milion at a production capacity of 100 kg/day. Break-even is reached at 489 kg/month (approximately 5 production days), with a payback period of approximately 6 months, assuming an initial investment of IDR 50 million, confirming its viability as a mainstream healthy alternative grain. Red sorghum, with a production cost of 16.464/kg and a selling price of IDR 25,000/kg, offers the highest contribution margin among all cultivars (34.1%, IDR 8,536/kg), with a payback period of approximately 3 months, making it the most economically attractive for the premium functional food segment. Black sorghum at IDR 25,000/kg yields a margin of 17.4% (IDR 4,342/kg) with a payback period of approximately 6 months, remaining commercially viable for health-conscious consumers. Cream sorghum, however, requires a minimum selling price of IDR 18,900/kg to recover production costs, making it less competitive at the standard market price of IDR 15,000/kg and necessitating premium positioning or further processing efficiency to improve its economic viability.
Comprehensive evaluation of seven sorghum cultivars from East Nusa Tenggara revealed significant variability in physical quality, bioactive and anti-nutrient compounds and economic feasibility, with dark coloured cultivars showing higher bioactives but also higher anti-nutrients. White-red sorghum showed the highest economic efficiency (ratio 0.092) owing to the fastest cooking time and optimal resource use. White-red and yellow sorghum are prospective candidates for mainstream commercial production, balancing sensory acceptability, nutritional value and economic feasibility, while black and red sorghum suit the premium functional-food segment despite higher costs. These findings provide an empirical basis for cultivar selection and commercialisation strategies supporting sustainable food security in Indonesia.
The authors gratefully acknowledge the financial assistance provided by the Impactful Consortium Research Grant, Ministry of Higher Education, Science and Technology.
 
Disclaimers
 
The findings and interpretation are the author’s responsibility and do not necessarily reflect the views of their affiliated institutions.
 
Informed consent
 
This study did not involve human or animal subjects: data were obtained solely from sorghum seeds collected across East Nusa Tenggara Province, Indonesia. As the analysis covered only physical characteristics, bioactive compounds and economic viability, no ethical approval was required.
The authors declare no financial, professional, or personal conflicts of interest.

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