Optimization of multi-millet premix
Three distinct formulations (MMM S1, S2 and S3) were systematically developed with varying ingredient proportions to evaluate their influence on product quality and consumer acceptance. Following a comprehensive sensory evaluation, the S1 formulation demonstrated superior performance and was selected as the optimal combination. The finalized S1 formulation consisted of barnyard millet (
Echinochloa esculenta) 25%, finger millet (
Eleusine coracana) 20%, green gram (
Vigna radiata) 15%, seed mixture comprising flax seeds (
Linum usitatissimum), sunflower seeds (
Helianthus annuus) and pumpkin seeds (
Cucurbita pepo) 10%, milk powder 20%, cocoa powder (
Theobroma cacao) 5%, sodium bicarbonate 2% and xanthan gum 3%. The optimized S1 formulation was subsequently analyzed for physicochemical properties and nutritional composition.
The formulation success stemmed from strategic ingredient balancing. The 45% combined millet content represented a critical balance point. Previous research has established that millet incorporation beyond 50% in composite flour products often compromises sensory acceptability due to the accumulation of phenolic compounds that impart astringency
(Saleh et al., 2013). Green gram incorporation at 15% proved strategically important for protein enhancement. Pulse addition exceeding 20% in cereal-based products increases batter viscosity and prolongs cooking time due to modified starch-protein interactions and altered water absorption properties
(Bresciani et al., 2022). The 10% oilseed mixture served nutritional enhancement and functional improvement. This proportion falls within the range reported as optimal for omega-3 enrichment
(Kajla et al., 2015), particularly á-linolenic acid from flax seeds. Overall, S1 formulation achieved the essential balance between nutritional enhancement and organoleptic acceptability that determines consumer adoption of functional foods (
Bigliardi and Galati, 2013).
Sensory evaluation
Sensory characteristics of pancakes from three multi-millet premix formulations were evaluated using 9-point hedonic scale (Table 2). All formulations demonstrated high consumer acceptability with mean scores exceeding 8.0 across all attributes (Fig 3,4). Sample S1 exhibited superior sensory performance, achieving highest scores for taste/flavor (9.0±0.64), color (9.07±0.64), texture/mouthfeel (8.87±0.62) and overall acceptability (8.87±0.46). Based on comprehensive sensory assessment, Sample S1 was identified as optimal formulation with superior organoleptic properties and highest consumer preference.
Proximate composition analysis
The proximate composition analysis of the food sample revealed significant nutritional characteristics (Table 3). The moisture content was determined to be 2.32%, indicating a relatively dry product with good storage stability. This low moisture level is consistent with processed food products designed for extended shelf life and reduced risk of microbial growth. The total ash content of 3.24% represents the mineral content of the sample, it falls within the typical range for processed food products, indicating moderate mineral density. The relatively low ash content suggests that the product is not heavily fortified with mineral supplements.
The total carbohydrate content of 78.05% indicates that this product is predominantly carbohydrate-based, characteristic of cereal or grain-derived foods. This high carbohydrate percentage suggests the product could serve as a significant energy source in dietary applications. The sugar content analysis revealed 6.35% total sugar as sucrose, indicating moderate sweetness levels that may enhance palatability without excessive sugar loading. Protein content (13.04%) represented a substantial enhancement over conventional wheat-based pancake mixes (typically 5-7%), derived from synergistic contributions of barnyard millet (10.5%), finger millet (7-8%), green gram (24%), milk powder (26%) and oilseeds (18-25%). The enhanced protein content aligns with findings from similar millet-based composite mixes reported in the literature (
Baruah, 2024). This multi-source protein approach provides improved amino acid balance through complementarity, where green gram supplies lysine (6.8-7.2 g/100 g protein) deficient in cereals, while millets contribute sulfur-containing amino acids lacking in legumes (
Shukla and Srivastava, 2014).
Fat content and fatty acid profile
The total fat content (3.35%) indicates a low-fat formulation with health-promoting lipid composition (Table 3): monounsaturated fatty acids 1.28% (38% of total fat), polyunsaturated fatty acids 0.96% (29%), saturated fatty acids 0.72% (21%) and trans fatty acids <0.1% (undetectable). This yields an unsaturated: saturated ratio of 3.1:1 and a PUFA:SFA ratio of 1.33:1, substantially exceeding cardiovascular disease prevention recommendations. The predominance of unsaturated fats stems from the strategic oilseed blend, with flax seeds providing α-linolenic acid (estimated at 0.15-0.25% ALA), which is rare in cereal-based foods
(Goyal et al., 2014). The resulting omega-6:omega-3 ratio approximates 3.2:1, approaching the recommended 1-4:1 for optimal health outcomes, whereas Western diets typically exceed 10:1 (
Simopoulos, 2016).
The absence of detectable trans fats represents a significant nutritional advantage, as each 2% energy increase from trans-fat is associated with 23% increased coronary heart disease risk
(Mozaffarian et al., 2010). The strategic inclusion of oilseeds not only enriches the fatty acid profile but also contributes to the overall functional properties of the premix, enhancing texture and mouthfeel while providing essential fatty acids that support cardiovascular health and reduce inflammation.
Energy content
The calculated energy value of 394.5 kcal per 100 g indicates high-energy-density product, primarily from substantial carbohydrate content. The high energy density makes this premix particularly relevant for addressing energy-protein malnutrition in vulnerable populations. From nutritional security perspective, energy-dense millet-based foods provide sustained energy through complex carbohydrates while delivering essential micronutrients often deficient in energy-dense processed foods.
Fiber content
Crude fiber content of 2.24% represents moderate dietary fiber contribution, consistent with processed millet-based products. While not exceptionally high, this fiber level provides meaningful health benefits when contextualized within total dietary intake patterns. A 50 g serving would contribute approximately 1.12 g (4-5% of daily 25-30 g requirements), complementing fiber from other dietary sources.
Cholesterol and sodium analysis
Cholesterol content was below detectable limits (<5 mg per 100 g), indicating suitability for cholesterol-restricted diets. However, sodium content of 358 mg per 100 g is relatively high and should be considered in context of daily sodium intake recommendations, particularly for individuals with hypertension or cardiovascular concerns.
Antioxidant activity
Antioxidant activity analysis (1.85 PPM) indicates presence of bioactive compounds at moderate levels. Recent reports indicate that millets contain significant antimicrobial, anti-inflammatory, antiviral and anticancer properties
(Khan et al., 2025). Total phenolic content in minor millets ranges from 53.28 to 110.42 mg GAE/g with corresponding antioxidant activities
(Jenipher et al., 2024).
Shelf Stability and commercial viability
Accelerated shelf-life testing at 40±2°C and 75±5% relative humidity revealed projected shelf life of 6 months when stored in cool and dry conditions (Table 3). Quality parameters including moisture content, water activity and microbial count remained within acceptable limits throughout testing. The 6-month shelf life is commercially viable for distribution, retail and consumer use while ensuring product safety and quality maintenance.
Implications for food security
Millets are recognized for their climate resilience, thriving in arid and semi-arid conditions, this agricultural advantage, combined with their superior nutritional profile, positions millets as crucial for achieving Sustainable Development Goals, particularly SDG 2 (Zero Hunger), SDG 3 (Good Health and Well-being) and SDG 12 (Responsible Consumption and Production)
(Kumar et al., 2024). Urbanization has driven a dietary transition toward high-energy, nutrient-poor convenience foods (
Agregán et al., 2023). Products like this premix offer a health-promoting alternative.
Limitations
Several limitations warrant acknowledgment. The study did not quantify specific bioactive compounds. Also, glycemic index is required for empirical determination through clinical trials. This, long-term consumer acceptance studies across diverse demographic groups would strengthen market viability assessments.