Asian Journal of Dairy and Food Research, volume 38 issue 2 (june 2019) : 114-120

Probiotic Potential of Noni and Mulberry Juice Fermented with Lactic Acid Bacteria

Aditya Chaudhary, Vishnu Sharma, Baljeet S Saharan
1Department of Veterinary and Agricultural Sciences, Jayoti Vidyapeeth Women's University, Jaipur, Rajasthan, India.
Cite article:- Chaudhary Aditya, Sharma Vishnu, Saharan S Baljeet (2019). Probiotic Potential of Noni and Mulberry Juice Fermented with Lactic Acid Bacteria. Asian Journal of Dairy and Food Research. 38(2): 114-120. doi: 10.18805/ajdfr.DR-1435.
The present study assesses the feasibility of noni and mulberry as a raw substrate for the production of probiotic noni and mulberry juice by lactic acid bacteria (Lactobacillus plantarum SK-3 and Pediococcus acidilactici M-3). Changes in pH, titratable acidity (lactic acid), cell survival, and antioxidant properties were examined during fermentation. Both the strains grew well in noni juice and mulberry juice after 48 hour fermentation. P. acidilactici M-3 produced less lactic acid than L. plantarum SK-3. After 28 days of cold storage, both tested strains survived the low-pH conditions in fermented noni juice and mulberry juice. Both the juices fermented with L. plantarum SK-3 had a high antioxidant capacity. The noni and mulberry juice fermented with L. plantarum SK-3 had shown the cholesterol-lowering ability better than the juices fermented with P. acidilactici M-3. Finally, L. plantarum SK-3 and P. acidilactici M-3 were found as optimal probiotics for fermentation with noni juice as well as mulberry juice. In this investigation, the results could be an indicator of the development of health-promoting food juices.
  1. Angelov, A., Gotcheva, V., Hristozova, T., Gargova, S. (2005). Application of pure and mixed probiotic lactic acid bacteria and yeast cultures for oat fermentation. J Sci Food Agric. 85:2134_2141.
  2. AOAC. (1984). Official methods of analysis of the association of official analytical chemist, Hortwits W (ed). Association of official analytical chemists, Washington, D. C. USA.
  3. Arabshahi-Delouee, S., Urooj, A. (2007). Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem. 102(4):1233-1240. 
  4. Argyri, A.A., Nisiotou, A.A., Mallouchos, A., Panagou, E.Z., Tassou, C.C. (2013). Performance of two potential probiotic Lactobacillus strains from the olive microbiota as starters in the fermentation of heat shocked green olives. International Journal of Food Microbiology. 171:68–76. 
  5. Basu, B., Hazra, B. (2006). Evaluation of nitric oxide scavenging activity, in vitro and ex vivo, of selected medicinal plants traditionally used in inflammatory diseases. Phytother Res. 20:896_900.
  6. Calzuola, I., Gianfranceschi, G.L., Marsili, V. (2006). Comparative activity of antioxidants from wheat sprouts, Morinda citrifolia, fermented papaya and white tea. Int J Food Sci Nutr, 57:168_177.
  7. Chan-Blanco, Y., Vaillant, F., Pe´rez, A.M., Belleville, M., Zu´n˜iga, C., Brat, P. (2007). The ripening and aging of noni fruits (Morinda citrifolia L.): Microbiological flora and antioxidant compounds. J Sci Food Agric. 87:1710-1716.
  8. Chen, I.N., Ng, C.C., Wang, C.Y., Chang, T.L. (2008). Lactic fermentation and antioxidant activity of Zingiberaceae plants in Taiwan. Int J Food Sci Nutr. 22:1-10.
  9. Choi, I.H., Noh, J.S., Han, J-S., Kim, H.J., Han, E-S., Song, Y.O. (2013). Kimchi, a fermented vegetable, improves serum lipid profiles in healthy young adults: Randomized clinical trial. J Med Food. 16(3):223-229.
  10. Chong, T.M., Abdullah, M.A., Fadzillah, N.M., Lai, O.M., Lajis, N.H. (2004). Anthraquinones production, hydrogen peroxide level and antioxidant vitamins in Marinda elliptical cell suspension cultures from intermediary and production medium strategies. Plant cell reports. 22:951-958.
  11. Darias-Martin, J., Lobo-Rodrigo, G., Hernandez-Cordero, J., Diaz-Diaz, E., Diaz-Romero, C. (2003). Alcoholic beverages obtained from black mulberry. Food Technol. Biotechnol. 41(2):173-176.
  12. David B Fankhauser. (2005). Pour plate technique for bacterial enumeration. Uni. Of Cincinnati Clermont College. Batavia OH. 45103:1-3.
  13. Duh, P.D., Yen, G.C. (1997). Antioxidative activity of three herbal water extracts. Food Chem. 60:639-645.
  14. Ercisli, S., Orhan, E. (2007). Some physicochemical characteristics of black mulberry (Morus nigra L.) genotypes from Northeast Anatolia region of Turkey. Sci. Hortic. 116: 41-46.
  15. Ercisli, S., Tosun, M., Duralija, B., Voæa, S., Sengul, M., Turan, M. (2010). Phytochemical Content of Some Black (Morus nigra L.) and Purple (Morus rubra L.) Mulberry Genotypes. Food Technology & Biotechnology. 48(1).
  16. Fratianni, F., Pepe, S., Cardinale, F., Granese, T., Cozzolino, A., Coppola, R., Nazzaro, F. (2014). Eruca sativa might influence the growth, survival under simulated gastrointestinal conditions and some biological features of Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus rhamnosus strains. International Journal of Molecular Sciences. 15:17790–17805. 
  17. Imran, M., Talpur, F.N., Jan, M.S., Khan, A., Khan, I. (2007). Analysis of nutritional components of some wild edible plants. J. Chem. Soc. Pak.. 29(5):500-508.
  18. Kamiya, K., Tanaka, Y., Endang, H., Umar, M., Satake, T. (2004). Chemical constituents of Morinda citrofolia fruits inhibit copper-    induced low-density lipoprotein oxidation. J Agric Food Chem. 52:5843-5848.
  19. Khan, T., Ahmad, M., Khan, R., Khan, H., Ejaz, A., Choudhary, M.I. (2006). Evaluation of phytomedicinal potentials of selected plants of Pakistan. Am. Lab. 38(9):20-22.
  20. Lale, H., Ozcagiran, A. (1996). Study on pomological, phenologic and fruit quality characteristics of mulberry (Morus sp.) species, Derim. 13: 177–182 (in Turkish).
  21. Liong, M.T. and Shah, N.P. (2005). Acid and bile tolerance and cholesterol removal ability of Lactobacilli strains. Journal of Dairy Science. 88: 55-56.
  22. Luckow, T., Delahunty, C. (2004). Which juice is ‘healthier’? A consumer study of probiotic non-dairy juice drinks. Food Quality and Preference. 15:751-759.
  23. Marhamatizadeh, M.H., Rezazadeh, S., Kazemeini, F., Kazemi, M.R. (2012). The study of probiotic juice product conditions supplemented by the culture of Lactobacillus acidophilus and Bifidobacterium bifidum. Middle-East J Sci Res. 11(3):287-295. 
  24. Miller, N.J. and Rice-Evans, C.A. (1997). The relative contributions of ascorbic acid and phenolic antioxidants to the total antioxidant activity of orange and apple fruit juices and blackcurrant drink. Food Chem. 60:331-337.
  25. Nagpal, R., Ashwani, K. and Kumar, M. (2012). Fortification and fermentation of fruit juices with probiotic lactobacilli, Annals of Microbiology. 62(4):1573-1578.
  26. Natural news. Lowering cholesterol with fermented foods. (2012). http://www.Nyrnatural news.com/ diet-2/2012/11/lowering-    cholesterol-with-fermented-foods.
  27. Nazzaro, F., Fratinni, F., Sada, A., Orlando, P. (2008). Synbiotic potential of carrot juice supplemented with Lactobacillus spp. and inulin or fructooligosaccharides. J Sci Food Agric. 88:2271-2276.
  28. Norušis, M.J. (1993). SPSS Advanced Statistics User’s Guide. SPSS, Chicago.
  29. Pawlowska, AM., Oleszek, W., Braca, A. (2008). Qualiquantitative analyses of flavonoids of Morus nigra L. and Morus alba L. (Moraceae) fruits. J. Agric. Food Chem. 56: 3377–3380.
  30. Pereira, A.L.F., Maciel, T.C., Rodrigues, S. (2011). Probiotic beverage from cashew apple juice fermented with Lactobacillus casei. Food Res Int. 44: 1276–1283. 
  31. Rößle, C., Auty, M.A.E., Brunton, N., Gormley, R.T., Butler, F. (2010). Evaluation of fresh-cut apple slices enriched with probiotic bacteria. Innova Food Sci Emerg Technol. 11: 203–209. 
  32. Ross, I.A. (2001). Medical Plants of the World. Chemical Constituents, Traditional and Modern Medical Uses. Humana Press, New Jersey.
  33. Sadler, G.D., Murphy, P.A. (2010). pH and titrable acidity. In: Nielsen, S. (Ed.): Food analysis. 4th edition. New York, Springer US; 219–238.
  34. Sheehan, V.M., Ross, P., Fitzgerald, G.F. (2007). Assessing the acid tolerance and the technological robustness of probiotic cultures for fortification in fruit juices. Innovative Food Science and Emerging Technologies. 8:279–284.
  35. Shimada, K., Fujikawa, K., Yahara, K., Nakamura, T. (1992). Antioxidative properties of xanthan on the autooxidation of soybean oil in cyclodextrin. J Agric Food Chem. 40:945-948.
  36. Su, B.N., Pawlus, A.D., Jung, H.A., Keller, W.J., McLaughlin, J.L., Kinghorn, A.D. (2005). Chemical constituents of the fruits of Morinda citrifolia (Noni) and their antioxidant activity. J Nat Prod. 68:592-595.
  37. Tien, Y.Y., Ng, C.C., Chang, C.C., Tseng, W.S., Kotwal, S., Shyu, Y.T. (2005). Studies on the lactic-fermentation of sugar apple (Annona squamosa L.) puree. J Food Drug Anal. 13:377-381.
  38. Tripathi, M.K. and Giri, S.K. (2014). Probiotic functional foods: Survival of probiotics during processing and storage. Journal of Functional Foods. 9: 225–241.
  39. Tuorila, H., Gardello, A.V. (2002). Consumer response to an off flavour in juice in the presence of specific health claims. Food Quality and Preference. 13:561–569.
  40. Wang, C.H., Lai, P., Chen, M.E. and Chen, H.L. (2008). Antioxidative capacity produced by Bifidobacterium- and Lactobacillis acidophilus-mediated fermentations of konjac glucomannan and glucomannan oligosaccharides. J Sci Food Agric. 88:1294-1300.
  41. Wang, S.C., Chang, C.K., Chan, S.C., Shieh, J.S., Chiu, C.K. and Duh, P.D. (2014). Effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterol. Asian Pacific Journal of Tropical Biomedicine. 4(7): 523-528.
  42. Yahyahi and Soofyani, Z. (2014). The evaluation of production of drink based on malt extract and red probiotic fermentation fruit. MA thesis of food industry. Agriculture school. Azad University of Varamin Pishva branch.

Editorial Board

View all (0)