<div style="text-align: justify;">Today, the ecosystem is being rapidly destroyed due to habitat loss, animal hunting, road construction, raising of transmission lines, general pollution, and agricultural activities, in addition to many other factors, all of which directly and indirectly affect wildlife. By necessity, rehabilitation units are available for the care and treatment of wild birds and mammals in need of life support. This paper discusses data collected regarding the treatment and rehabilitation of wild mammals and birds brought to the Center for Wildlife Conservation and Rehabilitation at Van Y&uuml;z&uuml;nc&uuml; Y&yacute;l University from 2009-2016. During the course of the present study, total 145 wild animals including 117 birds and 28 mammals. Out of the total treated, 96 wild birds and 21 wild mammals recovered, 12 birds and 4 mammals died without responding to treatments, whereas 9 birds and 3 mammals were euthanized. Additionally, of the 96 birds and 21 mammals that recovered, 15 birds and 6 mammals were determined to be unable to survive in their natural habitats, and were sent to zoo gardens or wildlife conservation and rehabilitation centers. The remaining 96 animals that fully recovered were released back into their natural habitats.</div>

Treatment and Rehabilitation of Wild Birds and Mammals

<div>Plasma also called as &lsquo;fourth state of matter&rsquo; encompasses ionized gas, atoms, free molecules, radicals and free electrons. Considering its discovery in late 19th century plasma is extensively used as low pressure or high pressure plasmas in many applications commercially such as microelectronic technology, textile industry, organic waste management etc. When a gas is passed through an electric field in a plasma chamber, three types of collisions are known to take place in it particularly, excitation, ionization and deposition that provide plasma its characteristic glow, ion-electron pair, reactive species respectively. Plasma treatment of different object can change their chemical and physical properties. In Seed Science and Research, many seed quality enhancement techniques are used to improve seed quality in crop plants such as, seed priming, fortification, solid matrix priming, chemical treatment, hardening etc. Plasma treatment of seeds is a unique tool that utilizes an ionized gas to modify the physical and chemical properties seed viz., wettability, porosity, water absorption, activity of antioxidant enzymes. It can also decontaminate the seed surface off micro flora and convert hydrophobic seeds to hydrophilic. It also enhances soluble sugar content, protein content and causes a reduction in lipid peroxidation. Thus plasma treatment in turn improves seed germination rate, seedling characteristics, seed physical quality and seed health.</div>

<div>Plasma Treatment and Seed Quality Advancement: A Review</div>

<div>Background: Micronutrient formulation is a liquid mixture of micronutrients which can be used as a fertilizer. It is a mixture of two solutions viz., solution A and solution B. Solution A is a mixture of zinc sulphate (50 gL-1), boric acid (10 gL-1), copper sulphate (20 gL-1), manganous sulphate (0.5 gL-1), ferrous sulphate (10 gL-1) and ammonium molybdate (0.5 gL-1) whereas solution B is an organic acid which act as a chelate. The mixture can be diluted to specific concentrations for application. The objective of the study was to evaluate the effect of micronutrient formulation as seed treatment and foliar nutrition.&nbsp; 
 
Methods: The study was conducted at College of Agriculture, Padannakkad, Kerala Agricultural University and Regional Agricultural Research Station, Pilicode during 2018&ndash;19. The investigation was carried out in three parts: (i) standardization of micronutrient formulation for cowpea (ii) seed treatment study (iii) field experiment. The proportion of mixing micronutrient formulation and the duration for seed treatment was standardized in experiment I. In experiment II, a seed treatment study was carried out in a completely randomised design with seven treatments and three replications which included seed treatment with micronutrient formulation @ 0.25, 0.50, 0.75, 1, 1.5 and 2 percent as T1, T2, T3, T4, T5 and T6 respectively. T7 (seed treatment with water) was the control. Three, a field experiment was carried out in randomized block design (factorial) with twelve treatments and three replications. The treatment consisting of combination of four levels of seed treatment - factor A [no seed treatment (S1), seed treatment with rhizobium (S2), seed treatment with best concentration from experiment 1 (S3) and second best seed treatment from experiment 1 (S4)]&nbsp;and three levels of foliar application of micronutrient - factor B [no foliar application (F1), one foliar application at 15 DAS (F2) and two foliar applications at 15 and 30 DAS (F3)]. 
 
Result: Through lab experiments, solution B was standardized and the formulation was developed by mixing solution A and solution B in the ratio 1:2. Duration for seed treatment was also standardized as 6 hours. Thus micronutrient formulation was standardized. In seed treatment study, seeds treated with 2% micronutrient formulation (T6) showed highest plant height at three leaf stage (24.23 cm) and seedling vigour index (2423). In the field experiment seed treatment with 2% micronutrient formulation (S3), foliar spray at 15 and 30 days after sowing (F3) and their interaction (S3F3) was found to be highly effective to the crop in terms of plant height, stem diameter, number of pods per plant, pod weight per plant, grain yield and nutrient content in grain. These results clearly indicate the role of micronutrient formulation in enhancing the growth, yield and nutrient content of cowpea.</div>

Evaluation of Micronutrient Mixture as Seed Treatment and Foliar Nutrition in Grain Cowpea

<div style="text-align:justify">Background: Green gram is the important pulse crop of India and grown under extensive agroclimatic condition in India for its versatile uses. Moreover, extreme and inappropriate use of inorganic nutrients deteriorated the soil quality by reducing the soil microbial population and also declined the quality of crops. Now a days, majority of population living in urban and rural area had demand for safe and healthy food for which inclusive food production technology needs to be developed with priority on quality enhancement and yield stability. In fact, it is not possible for farmers to directly shift from inorganic source of nutrients to organics in their crop production as it may lead to decrease in crop yields in the initial years. This may be possible due to substitution of organic sources of nutrients in place of inorganics to fulfil the crop nutrient requirement for accomplishing higher and stable crop yield of better quality with upgradation in soil health. Therefore, the present study was carried out to evaluate the influence of differential substitution of nutrients through organics on growth, yield and nutrient content of green gram (Vigna radiata) in irrigated sub-tropical region of J and K. 
 
Methods: The experiment was conducted for two consecutive years in field during summer 2016 and 2017 at research farm of division of Agronomy located at latitude and longitude of 32&deg;40&#39; N and 73&deg;64&#39; E at Skuast-Jammu. Experiment was laid out with 16 treatments and three replications. Green gram variety &ldquo;SML-668&rdquo; was grown as per their respective recommended packages of cultivation except for the nutrient-N and its organic sources which were applied as per the treatments. Nutrient content of green gram was worked out in the laboratory following standard procedures. 
 
Result: The pooled result of the two-year study of green gram concluded that significantly highest growth parameters and yield of green gram were recorded with treatment 100% recommended dose of fertilizer whereas significantly lowest value of all these parameters were recorded with treatment 100% NPK through FYM. Numerically highest value of NPK content in seed and stover of green gram after two-year experiment was recorded with treatment 100% NPK through FYM whereas highest gross returns, net returns and b:c ratio were recorded with treatment 100% recommended dose of fertilizer when compared with other treatments.</div>

Influence of Differential Substitution of Nutrients through Organics on Growth, Yield and Nutrient Content of Green Gram (Vigna radiata) in Irrigated Sub-tropical Region of J and K

<div>Induced Pluripotent stem cells (iPSC) have a high ability to renew and differentiate themselves into various lineages and as vehicles of cell based therapy. Stem cell can differentiate under appropriate in vitro and in vivo conditions into different cell types. This study described the establishment of condition for in vitro expression of alpha MHC gene in cardiac differentiated canine iPSC (ciPSC). In vitro differentiation of canine iPSCs via embryoid bodies (EBs) were produced by &lsquo;Hanging Drop&rsquo; method. EB&rsquo;s were differentiated by using IMDM differentiation media: FBS &ndash; 10%, NEAA (100X) &ndash; 0.5%, &Acirc;-Mercaptoethanol- 100mM, Gentamycin- 5&micro;g/ml supplemented with Azacytidine- 0.5&micro;M. During differentiation, EBs were collected on day 4, 6, 8, 12, 16, 20 and 24 for characterization of cardiomyocytes specific marker expression. Total RNA from EBs were extracted by using Trizol method and subsequently cDNA were synthesized. The differentiated cells expressed cardiac specific gene (Alpha MHC) which started from day 6 of differentiation upto day 24 Immunocytochemistry and relative expression of cardiac specific genes revealed that ciPSC have the potential to differentiate into cardiomyocytes which can be used for cardiac tissue regeneration and as disease models for pharmaceutical testing.</div>

<div>Expression of Cardiac Specific Cell Marker in Ex Vivo Differentiated Canine iPSC</div>

Background: Homeostasis in adult body is maintained by stem cells which is a powerful reservoir harbored in the body which regenerate tissue and in response to disease and injury.Hair follicle is a dynamic mini organ supporting important biological functions of the body in maintaining homeostasis and skin tissue self-renewal. Hair follicle protects the body from cold and potential injuries. To know the pluripotency nature of hair follicle stem cells, a study was conducted on canine HFSCs (cHFSCs). 
&nbsp; 
Methods: In-vitro culture of cHFSCs was done by William&rsquo;s E medium with several growth factors and passaged. Stemness of the cultured cells was assessed by immunostaining of cells with Anti-cytokeratin 15 (CK15) marker. Viability of cells was checked by acridine orange staining and passage 2 cells were induced for adipogenic differentiation using adipogenic induction media for a period of seven days. 
 
Result: A spindle shaped cell with accumulation of fat droplets was observed in differentiated cHFSCs. Differentiated cHFSCs were positively stained for Oil Red &lsquo;O&rsquo; staining. Thus, this research proves that cHFSCs were multipotent cells which can be differentiated into many other type of cells such as adipocytes, chondrocytes, etc.

Adipogenic Differentiation of Canine Hair Follicle Stem Cells (cHFSCs)

Postpartum uterine infections result from uterine contamination with bacteria during parturition. The uterine infection implies adherence of pathogenic organisms to the mucosa, colonization or penetration of the epithelium, and release of bacterial toxins that lead to establishment of uterine diseases. Treatment of endometritis with parenteral or intra-uterine infusion of the antibacterial agents and antibiotics have met with varying degree of success but inconsistence of recovery rate, high cost of treatment, milk disposal after antibiotic therapy, immergence of microbial resistance are obvious disadvantage of their use. They also markedly destroy the phagocytic activity of polymorphonuclear leucocytes, which are responsible for maintaining non-specific uterine defence. So, there is urgent need to find out an alternative therapy for treatment of uterine infections by activation of natural defence mechanism in the uterus. Prospects in use of certain non-specific immunomodulators in routine therapeutic protocol for endometritis and other uterine disorders is reviewed in present studies.

Strategic treatment with immunomodulators to resolve&nbsp;endometritis in cow: A review

<div>Background: The impact method was used to make unilateral femoral comminuted fractures in tree shrews, which were then intravenously injected with lipopolysaccharide to create a traumatic systemic inflammatory response syndrome model.&nbsp; 
 
Methods: The treatment group was infused with tree shrew umbilical cord mesenchymal stem cells via the tail vein. After 10 days of treatment, 6 tree shrews were killed in the control, model and treatment groups and pancreatic tissue was collected for metabonomic analysis.&nbsp; 
 
Result: The 25 differential metabolites in the normal, model and stem cell treatment groups showed a trend of first decreasing and then increasing and 11 metabolites showed a trend of first increasing and then decreasing. These findings are in line with the recovery of the curative effect of the stem cell therapy model group.</div>

Metabonomic Method to Analyze the Changes in Pancreatic metabolites in Tree Shrew Traumatic Systemic Inflammatory Response Syndrome After Treatment with Umbilical Cord Mesenchymal Stem Cells&nbsp;

<div>Background: Green gram is one of the ancient and third important conventional pulse crop cultivated throughout India and acclimatized over wide range of agro-climatic zones for its multipurpose uses. Furthermore, imbalanced and inappropriate use of inorganic nutrients worsened the soil resource base by reducing the population of beneficial micro-organisms and also deteriorated the quality of the crops. Increased health consciousness among the masses has augmented the demand for safe and quality foods for which a comprehensive food production technology needs to be developed with emphasises on quality enhancement and yield stability. In fact, it is not affordable for an average Indian farmer to jump immediately from inorganic source of nutrients to organics in their crop production as it may lead to unbearable drastic reduction in crop yields in the initial years. This may become possible through the progressive substitution of organic sources of nutrients in place of in organics to meet crop nutrient requirement for attaining higher and stable crop yield of better quality with an improvement in soil health. Therefore, the present study was carried out to evaluate the effects of differential substitution of nutrients through organics on growth, yield, quality, nutrient uptake and economics of green gram in Shiwalik foothill region. 
 
Methods: In this field experiment conducted during summer 2016 and 2017, at SKUAST-Jammu, Main Campus Chatha with green gram variety SML-668 on sandy clay loam soil. The experiment was laid out in randomized block design with sixteen treatments during both the years using recommended package and practices. 
 
Result: The result of the study concluded that RDF and 75% NPK +25% N through Vermicompost and FYM (1:1) were adjudged as the best treatments with regard to crop growth parameters, yield and yield attributes, net returns and benefit cost ratio where as highest crop quality traits were realized where in 100% N was substituted through FYM followed by Vermicompost and Vermicompost and FYM (1:1). Treatment 100% N was substituted through FYM was adjudged as the best treatment in improving crop quality traits and soil properties. Further, for substitution of nutrients, green gram, for immediate shifting from in organics to organics combination of Vermicompost and FYM (1:1) can be the best option for early realization of yield at par with recommended dose of fertilizer.</div>

<div>Effect of Differential Substitution of Nutrients through Organics on Growth, Quality, Nutrient Uptake and Economics of Green Gram (Vigna radiata) in Shiwalik Foothill Region</div>

The bio-efficacy of imidacloprid seed treatment against leafhopper and thrips of groundnut was studied during kharif (23rd to 42nd standard week) and summer (5th to 23rd standard week) cropping seasons of 2010 and 2011. The six different concentrations viz., 0.5, 1.0, 2.0, 3.0, 4.0 and 5.0 g a. i. kg-1 were tested for their bio-efficacy. A modified sweep net method was followed to record the leafhopper and thrips populations. Seed treatments with imidacloprid @ 2.0 to 5.0 g a. i. kg-1 were found most effective in reducing the insect population. However, seed treatment with imidacloprid @ 2.0 g a. i. kg-1 may be included in integrated pest management (IPM) package for groundnut from environmental safety point.

Treatment and Rehabilitation of Wild Birds and Mammals

Plasma Treatment and Seed Quality Advancement: A Review

Evaluation of Micronutrient Mixture as Seed Treatment and Foliar Nutrition in Grain Cowpea

Influence of Differential Substitution of Nutrients through Organics on Growth, Yield and Nutrient Content of Green Gram (Vigna radiata) in Irrigated Sub-tropical Region of J and K

Expression of Cardiac Specific Cell Marker in Ex Vivo Differentiated Canine iPSC

Adipogenic Differentiation of Canine Hair Follicle Stem Cells (cHFSCs)

Strategic treatment with immunomodulators to resolve endometritis in cow: A review

Metabonomic Method to Analyze the Changes in Pancreatic metabolites in Tree Shrew Traumatic Systemic Inflammatory Response Syndrome After Treatment with Umbilical Cord Mesenchymal Stem Cells

Effect of Differential Substitution of Nutrients through Organics on Growth, Quality, Nutrient Uptake and Economics of Green Gram (Vigna radiata) in Shiwalik Foothill Region

Efficacy of imidacloprid seed treatment for the control of leafhoppers and thrips in groundnut