Agricultural Reviews

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Agricultural Reviews, volume 37 issue 3 (september 2016) : 186-195

Strategic treatment with immunomodulators to resolve endometritis in cow: A review

Pradip Sarkar*1, Manas Kumar Patra2, Harendra Kumar
1<p>Animal Reproduction Division,&nbsp;Indian Veterinary Research Institute, Izatnagar-243 122, U.P., India.</p>
Cite article:- Sarkar*1 Pradip, Patra2 Kumar Manas, Kumar Harendra (2016). Strategic treatment with immunomodulators to resolveendometritis in cow: A review . Agricultural Reviews. 37(3): 186-195. doi: 10.18805/ag.v37i3.3534.

Postpartum uterine infections result from uterine contamination with bacteria during parturition. The uterine infection implies adherence of pathogenic organisms to the mucosa, colonization or penetration of the epithelium, and release of bacterial toxins that lead to establishment of uterine diseases. Treatment of endometritis with parenteral or intra-uterine infusion of the antibacterial agents and antibiotics have met with varying degree of success but inconsistence of recovery rate, high cost of treatment, milk disposal after antibiotic therapy, immergence of microbial resistance are obvious disadvantage of their use. They also markedly destroy the phagocytic activity of polymorphonuclear leucocytes, which are responsible for maintaining non-specific uterine defence. So, there is urgent need to find out an alternative therapy for treatment of uterine infections by activation of natural defence mechanism in the uterus. Prospects in use of certain non-specific immunomodulators in routine therapeutic protocol for endometritis and other uterine disorders is reviewed in present studies.

  1. Agarwal, S.K. and Tomer, O.S. (2003). Reproductive technologies in buffalo. A monograph published by communication centre, Indian Veterinary research Institute, Izatnagar, U.P., India.

  2. Arthur, G.H., Noakes, D.E. and Pearson, H. (2009). In: Veterinary Reproduction and Obstetrics. 9th edn., Bailliere Tindall, London, pp. 384-389.

  3. Asbury, A.C., Gorman, N.T. and Foster, G. W. (1984). Uterine defence mechanism in mare: Serum opsonins affecting phagocytosis of Strep. zooepidermicuc by equine neutrophils. Theriogenology, 21: 375-385.

  4. Barlund, C.S., Carruthers, T.D., Waldner, C.L. and Palmer, C.W. (2008). A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology, 69: 714-723.

  5. Barman, P., Yadav, M.C., Bangthai, A. and Kumar, H. (2013). Antibiogram of bacteria isolated from bovine endometritis. Vet. Res. International, 1: 20-24.

  6. Bindrawan (2001). Efficacy of neem oil and colostral whey in treatment of endometritis cows. M.V. Sc. Thesis. I.V.R.I. 

  7. BonDurant, R.H. (1999). Inflammation in the bovine reproductive tract. |J. Dairy Sci., 82 (Suppl. 2): 101–    110.

  8. Bonnett, B.N., Martin, S.W., Gannon, V.P., Miller, R.B. and Etherington, W.G. (1991). Endometrial biopsy in Holstein – Friesian dairy cows. III. Bacteriological analysis and correlations with histological findings. Can. J. Vet. Res., 55: 168- 173.

  9. Borowieck, J. (1989). Therapeutic trial with an alcoholic plant extract in bovine puerperal endometritis. Medyena Weteryaryjna, 45: 221- 224.

  10. Borsberry, S. and Dobson, H. (1989). Periparturient diseases and their effect on reproductive performance in five dairy herds. Vet. Rec., 124: 217-219.

  11. Claesson, H.E., Odlander, B. and Jacobsson, P.J. (1992). Leukotriene B4 in the immune system. Int. J. Immunopharmac., 14: 441-449.

  12. Correa, M.T., Erb, H. and Scarlett, J. (1993). Path analysis for seven postpartum disorders of Holstein cows. J. Dairy Sci., 76: 1305.

  13. Decan, M. and Rosu, M. (1973). Investigation on normal and pathological microflora of cervical secretions in cattle. Revista de Zootechnie Si Medicina Veterinaria, 23: 39-46.

  14. Del Vecchio, R.P., Matsas, D.J., Frontin, S., Sponenberg, D.P. and Lewis, G.S. (1994). Spontaneous uterine infections are associated with elevated prostaglandin F2a metabolite concentrations in postpartum dairy cows. Theriogenology, 41:413-421.

  15. Deori, S. (2002). Use of bacterial modulins in treatment of endometritis cows. M.V.Sc. Thesis. I.V.R.I.

  16. Dhaliwal, G.S., Murray, R.D. and Woldehiwet, Z. (2001). Some aspects of immunology of the bovine uterus related to treatments for endometritis. Anim. Reprod. Sci., 67: 135-152.

  17. Dohmen, M.J.W., Lohuis, J.A.C.M., Huszenicza, G., Nagy, P. and Gacs, M. (1995). The relationship between bacteriology and clinical findings in cows with subacute/chronic endometritis. Theriogenology, 43: 1379- 1388.

  18. Dowlen, H. Murphree, R.L., Richardson, D.O. and Owen, J.R. (1983). Effect of immediate postpartum treatment on subsequent reproductive efficiency. Theriogenology, 19: 811- 815.

  19. Dubuc, J., Duffield, T.F., Leslie, K.E., Walton, J.S. and LeBlanc, S.J. (2010). Definitions and diagnosis of postpartum endometritis in dairy cows. J. Dairy Sci., 93: 5225-5233.

  20. Elliot, L., McMohan, K.J. and Gier, H.T. (1968). Uterus of the cow after parturition: Bacterial content. Am. J. Vet. Res., 29: 77-81.

  21. Fischer, C., Drillich, M., Odau, S., Heuwieser, W., Einspanier, R. and Gabler, C. (2010). Selected proinflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of sub-clinical or clinical endometritis. Reprod. Fert. and Develop., 22: 818-829.

  22. Foldi, J., Kulcs´ar, M., P´ecsi, A., Huyghe, B., de Sa, C., Lohuis, J.A.C.M., Cox, P. and Huszenicza, Gy. (2006). Bacterial complications of postpartum uterine involution in cattle. Ani. Rep. Sci., 96: 265–281.

  23. Fukushima, Y. (1972). A etiological factors concerned E. coli infections of the bovine female genital system. Bulletin of the Azabu Vet. Col. 23: 123-160.

  24. Gunnink, J.W. (1973). Investigations concerning the uterine defense mechaninsms. University of Utrecht, PhD Thesis, pp. 143-160.

  25. Galvão, K.N., Flaminio, M.J.B.F., Brittin, S.Z., Sper, R., Fraga, M., Caixeta, L., Ricci, A., Guard, C.L., Butler, W.R. and Gilbert, R.O. (2010). Association between uterine disease and indicators of neutrophil and systemic energy status in lactating Holstein cows. J. of Dairy Sci., 93: 2926-2937.

  26. Ganz, T., Selsted, M.E., Szklarek, D., Harwig, S.S.L., Daher, K., Bainton, D.F. and Lehrer, R.I. (1985). Defensins. Natural peptide antibiotics of human neutrophils. J. Clin. Invest., 76: 1427-1435.

  27. Gautam, G., Nakao, T., Yusuf, M. and Koike, K. (2009). Prevalence of endometritis during the postpartum period and its impact on subsequent reproductive performance in two Japanese dairy herds. Anim. Reprod. Sci., 116: 175-187.

  28. Gennaro, R., Scheidder, C., DE Nicola, G., Cian, F. and Romeo, D. (1978). Biochemical properties of bovine Granulocytes. Proc. Soc. Exp. Bio. Med., 157: 342- 347.

  29. Ghasemi, F. (2011). Characterization of endometritis in postpartum dairy cows. M.Sc. thesis submitted to Western college of Veterinary Science, University of Saskatchewan. 

  30. Gilbert, R.O. (1992). Bovine endometriris: The burden of proof. Cornett Vet., 82: 11-14.

  31. Gilbert, R.O., Shin, S.T., Guard, C.L., Erb, H.N. and Frajblat, M. (2005). Prevalence of endometritis and its effects on reproductive performance of dairy cows. Theriogenology, 64: 1879-1888.

  32. Goff, J.P. and Horst, R.L. (1997). Physiological changes at parturition and their relationship to metabolic disorders. J. of Dairy Sci., 80: 1260-1268.

  33. Gonzalez, H. E., Crowell, W. A., Caudle, A. B. and Thompson, F. N. (1985). Morphometric studies of the bovine uterus: Microscopic lesions and retrospective reproductive history. Am. J. Vet. Res., 46: 2588.

  34. Hanafi, E.M., Abdel-khader, M.M., Kassem, S.S. and Danial, E.N. (2010). Aroma-therapy for endometritis induced by candida albicans. Int. J. of Acad. Res., 2: 112-119.

  35. Heuwieser, W., Tenhagen, B.A., Tischer, M., Luhr, J. and Blum, H. (2000). Effect of three programmes for the treatment of endometritis on the reproductive performance of a dairy herd. Vet. Rec., 146: 338-341.

  36. Hoedemaker, M., Lund, L.A. and Wagner, W.C. (1992). Influence of arachidonic acid metabolites and steroids on function of bovine polymorphonuclear neutrophils. Am. J.Vet. Res., 53: 1534-1543.

  37. Hualand, M.A., Manspeaker, J.F. and Moreland, T.W. (1984). Antibiotic residues in milk after intrauterine infusion. Vet. Med. Sm. Anim. Clin., 79: 383- 386.

  38. Hughes, J.P. and Couto, M.A. (1988). Endometritis in mare. 11th Inter. Congr. Anim. Reprod. And A.I. Dublin, Ireland, 5: 91-99.

  39. Hussain, A.M. and Daniel, R.C.W. (1991). Bovine endometritis: Current and future alternative therapy. J. Vet. Med. Assoc., 38: 641-651.

  40. Hussain, A.M. and Daniel, R.C.W. (1992). Effects of intrauterine infusion of E. coli endotoxin in normal cows and in cows with endometritis induced by experimental infection with Strep. agalactiae. Theriogenology, 37: 791- 810.

  41. Jayappa, H. and Luken, K.I. (1983). Effect of antimicrobial agents and corticosteroids on bovine polymorphonuclear leucocytes chemotaxis. Am. J. Vet. Res., 44: 2155-2159.

  42. Jochle, W. (1998). 7th Int. Symp. on Equine Reprod., University of Pretoria, South Africa, July 12 to 17, pp. 1-14.

  43. Karreman, H.J., Wentink, G.H. and Wensing, T. (2000). Using serum amyloid A to screen dairy cows for subclinical inflammation. Vet. Quart., 22: 175-178.

  44. Kasimanickam, R., Duffield, T. F., Foster, R. A., Gartley, C. J., Leslie, K. E., Walton, J. S. and Johnson, W. H. (2005). A comparison of the cytobrush and uterine lavage techniques to evaluate endometrial cytology in clinically normal postpartum dairy cows. Can. Vet. Jou., 46: 255-259.

  45. Korf, J., Stoltz, A. Verschoor, J. De Baetselier, P. and Grooten, J. (2005). The mycobacterium tuberculosis cell wall component mycolic acid elicits pathogen-associated host innate immune response. Eur. J. Immunol., 35: 890-900.

  46. Krishnan, B.B. (2011). Effect of immunomodulators on the recovery of subclinical endometritis in crossbred cow. M.V.Sc. thesis. IVRI.

  47. Kumar, H., Singh, B., Goswami, T.K. and Rawat, M. (2013). Use of neem preparations for the treatment of endometritis cows. Adv. In Anim. And Vet. Sci., 1: 194-196.

  48. Kumar, P. Srivastava, S.K., Rawat, M., Yadav, M.C. and Kemar H. (2004). Effect of certain immunomodulators on uterine infections anf fertility in post partam buffaloes. Asian- Aust. J. Anim. Sci. 17: 930-935.

  49. LeBlanc, S.J., Duffield, T.F., Leslie, K.E., Bateman, K.G., Keefe, G.P., Walton, J.S., Johnson, W.H. (2002). Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. Journal of Dairy Sci., 85: 2223–2236.

  50. Lewis G.S. (2003). Steroidal regulation of uterine resistance to bacterial infection in livestock. Reproductive Biology and Endocrinology, 1: 117.

  51. Lewis, G. S. (1997). Uterine health and disorders. J. Dairy Sci., 80: 984-994.

  52. Lewis, G. S. (1997). Uterine health and disorders. J. Dairy Sci., 80: 984-994.

  53. Lewis, G.S. (2004). Steroidal regulation of uterine immune defences. Anim. Reprod. Sci., 82-83: 281-294.

  54. Loyi, T., Kumar, H., Nandi, S. and Patra, M.K. (2015). Expression of Pathogen Recognition Receptors and Pro-Inflammatory Cytokine Transcripts in Clinical and Sub-Clinical Endometritis Cows. Ani. Biotech. 26:194-200.

  55. Loyi, T., Kumar, H., Nandi, S., Mathapati, B.S., Patra, M.K., Pattnaik, B. (2012). Differential expression of pro-inflammatory cytokines in endometrial tissue of buffaloes with clinical and sub-clinical endometritis. Res. Vet. Sci. 94:336-40. 

  56. Massera, J., Gustafsson, B.K., Afiefy, M.M., Stowe, C.M. and Bergt, G.P. (1980). Deposition of oxytetracycline in the bovine genital tract: Systemic vs intrauterine administration. J. Am. Vet. Med. Assoc., 176: 1099-1102.

  57. Mateus, L., Lopes da Costa, L., Bernardo, F. and Robalo Silva, J. (2002a). Influence of puerperal uterine infection on uterine involution and postpartum ovarian activity in dairy cows. Reprod. Domestic Anim., 37: 31-35.

  58. Maurya, S.N., Dabas, Y.P.S. and Gupta, R.S. (1992). A note on bacteriological studies of cervical secretion of infertile cows and buffaloes. Ind. J. Ani. Reprod., 13: 49-50.

  59. Methai, A. (1999). Efficacy of lipopolysaccharide and plasma in the treatment of endometritis in cows. M.V. Sc. Thesis, Madras Veterinary College.

  60. Mollett, T.A., Elmore, R.G., Blanchard, T.L. and Berg, J.N. (1985) Effects of intrauterine infusion of E. coli endotoxin in anestrous and steroid treated pony mares. Theriogenology, 23: 597- 606.

  61. Noakes, D.E., Parkinson, T.Z. and England, G.C.W. (2001). Anthur’s Veterinary Reproduction and Obstetrics, W.B. Saunders Company, Pennsylvania. p.p.- 403.

  62. Olson, J.D., Ball, L. and Mortimer, R.G. (1985). Therapy of postpartum uterine infections. The Bovine Practice, 17: 85-88.

  63. Olson,J.D., Ball, L., Mortimer, R.G., Farin, P.W., Adney, W.S. and Huffman, E.M. (1984). Aspects of bacteriology and endocrinology of cows with pyometra and retained fetal membranes. Am. J. Vet. Res., 45: 2251-2256.

  64. Palmer C.W. (2008). Postpartum endometritis: Current concepts in diagnosis and treatment. 29th World Vet. Cong. 241-250.

  65. Patra, M K., Kumar, H. and Nandi, S. (2014). Differential cytokine expression profile in peripheral blood mononuclear cells of endometritic buffaloes. Indian J. Anim. Sci., 84:1265-1269.

  66. Polyantsev, N.I. and Popov, Yu. N. (1972). Diagnosis and treatment of occult endometritis in cows. Vastnek Selskoknozyanstvennoi Nauki, 1: 22-26.

  67. Popov, Yu. N. (1969). Diagnosis of occult endometritis in cows (Using white side test on cervical mucus) Vet. Moscow, 4: 85-87.

  68. Prasad, J.K., Saxena, M.S., Shiv Prasad. and Singh, G.K. (2009). Comparative efficacy of E. coli lipopolysachharide, oyster glycogen and enrofloxacin on uterine defence mechanism and fertility in crossbred cows with endometritis. Indian J. Anim. Sci., 79: 1111-1115.

  69. Raab, D. (2004). Evaluierung der Cytobrush-Methode zur Diagnostik von subklinischen Endometritiden und Auswirkungen der Entzündung auf die folgende Fruchtbarkeitsleistung von Milchkühen. FU-Berlin. Dissertation.

  70. Roberts, S.J. (1986). In: Veterinary Obstetrics and Genital Diseases. 3rd edn. Ed. S.J. Roberts. Woodstock. Vermont. pp. 538-546.

  71. Rogan, D., Fumoso, E., Rodryguez, E., Wade, J. and Sanchez Bruni, S.F. (2010). Use of a mycobacterial cell wall extract (MCWE) in susceptible mares to clear experimentally induced endometritis with Strep. zooepidermicus. J. Equine Vet. Sci., 27: 112-117.

  72. Saini, P.S. (1993). Studies on etio-pathology and modified therapy of bovine endometritis. M.V.Sc. Thesis, Punjab Agricultural University, Ludhiana.

  73. Saini, P.S., Nanda, A.S., grewal, A.S. and Singh, J. (1999). Uterine defence modulation for the treatment of repeat breeding due to infectious endometritis in bovines. Ind. J. Anim. Sci., 69: 307-309.

  74. Sande, M.A. and Mandell, G.I. (1980). Antimicrobial agents tetracyclines and chloromphenicol. In: The pharmacological Basis of Therapeutics. Gilman, A.G., Goodman, L,S. and Gilman, A. (eds), Macmillan Pub. Co., New York, pp. 1080-1105.

  75. Sarkar, P. (2004). Studies on effect of some non specific immunomodulators on recovery of endometritis in cross bred cows. M.V.Sc. thesis submitted to IVRI. Izatnagar.

  76. Sarkar, P., Kumar, H., Rawat, M., Varshney, V.P., Goswami, T.K., Yadav, M.C. and Srivastava, S.K. (2006). Effect of administration of garlic extract and PGF2a on hormonal changes and recovery in endometritis cows. Asian- Aust. J. Anim. Sci., 19: 964-969.

  77. Sheldon, I. M. and Dobson, H. (2004). Postpartum uterine health in cattle. Animal Reproduction Science, 82-83: 295-306.

  78. Sheldon, I.M., Lewis, G.S., LeBlanc, S. and Gilbert, R.O. (2006) Defining postpartum uterine disease in cattle. Theriogenology, 65: 1516-1530.

  79. Sheldon, I.M., Noakes, D.E., Rycroft, A.N., Pfeiffer, D.U. and Dobson, H. (2002). Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle. Reproduction, 123: 837-845.

  80. Shukla, P.S. and Pandit, R.K. (1989). Incidence of repeat breeding and its remedial measures in gir cows and their crosses. Ind. Vet. J., 66: 626: 630.

  81. Singh, B. (2005). Therapeutic efficacy of Neem (Azadirachta Indiaca) in cows with endometritis. M.V.Sc. Thesis. IVRI. 

  82. Singh, J. Sidhu, S.S., Dhaliwal, G.s., Pangoankar, G. R., Nanda, A.S. and Grewal A.S. (2000). Effectiveness of lipopolysaccharide as an intrauterine immunomodulator in curing bacterial endometritis in repeat breeding crossbred cows. Anim. Reprod. Sec., 59: 159- 166.

  83. Singh, J., Nanda, A.S., Dhaliwal, G.S. and Pangaonkar, G.R. (2003). Treatment of bacterial endometritis in crossbred cows using intrauterine oyster glycogen, a non specific immunomodulators. Ind. J. Anim. Sci., 73: 844-847.

  84. Singh, K.P., Singh, B., Singh, S.V., Singh, J.P., Singh, P. and Singh, H.N. (2011). Efficacy of pre and post AI infusion of gentamycin on conception rate in crossbred cows. Ind.Vet.J., 88: 70-71.

  85. Singla, P., Singh, J., Dhaliwal, G.S. and Kumar, A. (2004). Effect of post AI intrauterine treatment with E. coli lipopolysaccharide and autologous serum on characteristics of cervico-vaginal mucous in subclinical endometritic cows. Ind. J. Anim. Reprod., 25: 55-57.

  86. Skinner, J. G., Brown, R.A.L.I. and Roberts, L. (1991). Bovine haptoglobin response in clinically defined field conditions. Vet. Rec., 128: 147- 149.

  87. Smith, B.I., Donovan, G.A., Risco, C.A., Young, C.R. and Stanker, L.H. (1998). Serum haptoglobin concentration in Holstein dairy cattle with toxix puerperal metritis. Vet. Rec., 142: 83-85.

  88. Subandrio, A.L. and Noakes, D.E. (1997). Neutrophil migration into the uterine lumen of the cow: The influence of endogenous sex steroid hormones using two intrauterine chemoattractants. Theriogenology, 47: 825-835.

  89. Subandrio, A.L., Sheldon, I.M. and Noakes, D.E. (2000). Peripheral and intrauterine neutrophil function in the cow: the influence of endogenous and exogenous sex steroid hormones. Theriogenology, 53: 1591-1608.

  90. Talwar, G.P., Raghubanshi, P., Mishra, R., Mukherjee, S. and Shah, S (1997). Plant immunomodulators for termination of unwanted pregnancy and for contraception and reproductive health. Immunol. Cell Biology, 75: 190-192.

  91. Timothy, J.P., Javier, G., John, F., Patrick, J.G. and Sheldon, I.M. (2010). Risk factors for clinical endometritis in postpartum dairy cattle. Theriogenology, 74: 127- 134.

  92. Usmani, R.H., Ahmad, N., Shafiq, P., and Mirza, M.A. (2001). Effect of sub-clinical uterine infections on cervical and uterine involution, estrous activity and fertility in postpartum buffaloes. Theriogenology, 55: 563-571.

  93. Vandeplassche, M. and Bouters, R. (1983). Phagocytosis in the blood and uterine exudates of mares, cows and sows. Proc. 3rd Int. Sym. World Assoc. Vet. Lab. Diag., Ames, I.A.I., 83-89.

  94. Venugopal, R. (1995). Intra uterine infusion of homologous plasma in the treatment of endometritis in cows. M.V.Sc. Thesis, College of Veterinary and Animal Sciences, Mannuthy, Trissur.

  95. Ward, A. (1985). A new treatment for infertility in mares. Vet. Med., 80: 65-69.

  96. Whitemore, H.L. and Anderson, K.L. (1985). Possible adverse effectsof antimicrobial treatment of uterine infections. In: Current therapy in Theriogenology Ed. David A, Morrow, W.B. Saunders Company, PD. 42-44.

  97. Williams, E.J., Fischer, D.P., Pfeiffer, D.U., England, G.C.W., Noakes, D.E., Dobson, H. and Sheldon, I.M. (2005). Clinical evaluation of post partum vaginal mucus reflects uterine bacterial infection and the immune response in cattle. Theriogenology, 63: 102-117.

  98. Williamson, P., Munya, S., Martin, R. and Penhale, W.J. (1987). Dynamics of the acute uterine response to infection, endotoxin infusion and physical manipulation of the reproductive tract in the mare. J. Reprod. Fert., 35: 317-325.

  99. Yavas, Y.W.J.S. (2000). Postpartum acyclicity in suckled beef cows: A review. Theriogenology, 54: 25-55.

  100. Zain, A.E.D., EI Rallal, S.S. and Shehata, S.H. (1997). Alkaline phosphatase and transaminase activity in uterine flushing of buffalo cows affected with endometritis. Buffalow J., 13: 95-102.

  101. Zerbe, H., Schuberth, H.J., Engelke, F., Frank, J., Klug, E. and Leibold, W. (2003). Development and comparison of in vivo and in vitro models for endometritis in cows and mares. Theriogenology, 60: 209-223.

  102. Zerbe, H., Schuberth, H.J., Hoedemaker, M., Grunert, E. and Leibold, W. (1996). A new model system for endometritis: Basic concepts and characterization of phenotypic and functional properties of bovine uterine neutrophils. Theriogenology, 46: 1339-1356.

  103. Ziv, G. (1984). Minimal inhibitory concentrations of antibiotics for pathogenic bacteria as determined aerobically and anaerobically in uterine secretions. Proc. 10th Int. Cong. Anim. Reprod. and A.I., Urbana- Champaign, 1: 420.

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