Efficacy of Probiotic Bacteria (Lactobacillus plantarum) Supplementation in the Diet on Growth and Nutritional Composition of Cirrhinus mrigala Fingerlings

ABSTRACT

Background: The use of probiotics in aquaculture is increasingly acknowledged, thanks to rising demand for environment friendly aquaculture. However, there is an obvious need to improve our understanding of gut microbiology, as well as the effective preparation and safety assessment of probiotics.
Methods: The experiment comprised of five treatments to evaluate the influence of probiotic on water quality, growth and body composition. L. plantarum probiotic was sprayed over test diets at various concentrations @ 106 to 109 CFU/g, (D0 to D4 respectively). Water quality parameters were analysed (APHA, 2012) and growth parameters formulae (Halver, 1957) whereas fish feed and fish flesh nutritional composition, were measured (AOAC, 2012).
Result: All of the water quality parameters were within acceptable limits. Net weight gain (NWG), NWG %, specific growth rate, Condition factor and survival rate were significantly (P<0.05) highest in D4 and lowest in D0. Similarly, Feed conversion ratio and protein efficiency ratio also improved significantly (P<0.05). Moreover, there was a considerable rise in crude protein and lipid content in D4. In general, supplementation of L. plantarum @ 109cfu/g diet improved growth, survival and nutritional composition of Cirrhinus mrigala.

KEYWORDS

REFERENCES

Abumourad, I.M.K., Abbas, W.T., Awaad, E.S., Authman, M.M.N., El-Shafei, K., Ibrahim, O.M.S., Gamal, A., Sadek, Z.I., El-Sayed, H.S. (2013). Evaluation of Lactobacillus plantarum as a probiotic in aquaculture: Emphasis on growth performance and innate immunity. Journal of Applied Sciences Research. 9(1): 572-582.

Amit, Pandey, A., Khairnar, S.O., Tyagi, A. (2021). Effect of Dietary Supplementation of Probiotic Bacteria (Lactobacillus plantarum) on Growth and Proximate Composition of Cyprinus carpio Fingerlings. National Academy Science Letters-India. 44: 495-502.

Amit, Pandey, A., Tyagi, A., Khairnar, S.O. (2022). Oral feed-based administration of Lactobacillus plantarum enhances growth, haematological and immunological responses in Cyprinus carpio “, Emerging Animal Species. 3: 100003.

AOAC. (2012). Official Methods of Analysis of AOAC International. 19th edition. AOAC 54 International, Gaithersburg, Maryland, USA.

APHA. (2012). Standard Methods for the Examination of Water and Wastewater. 22ndEdn. American Public Health Association, Washington, D.C. 1360 p.

Barbosa, M.C., Jatobá, A., Vieira, F.N., Silva, B.C., Mourino, J.L., and andreatta, P.E.R., Seiffert, W.Q., Cerqueira, V.R. (2011). Cultivation of juvenile fat snook (Centropomus parallelus Poey, 1860) fed probiotic in laboratory conditions. Brazilian Archives of Biology and Technology. 54 (4): 795-801.

Chi, C., Jiang, B., Yu, X.B., Liu, T.Q., Xia, L., Wang, G.X. (2014). Effects of three strains of intestinal autochthonous bacteria and their extracellular products on the immune response and disease resistance of common carp, Cyprinus carpio. Fish and Shellfish Immunology. 36 (1):9-18.

Dimitroglou, A., Merrifield, D.L., Carnevali, O., Picchietti, S., Avella, M., Daniels, C., Guroy, D., Davies, S.J. (2011). Microbial manipulations to improve fish health and production-A Mediterranean perspective. Fish and Shellfish Immunology. 30:1-16.

Enferadi, M.H.N., Mohammadizadeh, F., Soltani, M., Bahri, A.H., Sheikhzadeh, N. (2018). Effects of Lactobacillus plantarum on growth performance, proteolytic enzymes activity and intestine morphology in rainbow trout (Oncorhynchus mykiss). Turkish Journal of Fisheries and Aquatic Sciences. 18: 351-356.

Gao, Q., Xiao, C., Min, M., Zhang, C., Peng, S., Shi, Z. (2016). Effects of probiotics dietary supplementation on growth performance, innate immunity and digestive enzymes of silver pomfret, Pampus argenteus. Indian Journal of Animal Research. 1-6.

Gatesoupe, F.J. (1999). The use of probiotics in aquaculture. Aquaculture. 180: 147-165.

Geng, X., Dong, X.H., Tan, B.P., Yang, Q.H., Chi, S.Y., Liu, H.Y., Liu, X.Q. (2012). Effects of dietary probiotic on the growth performance, non-specific immunity and disease resistance of cobia, Rachycentron canadum. Aquaculture Nutrition. 18 (1): 46 55.

Ghosh, K., Sen, S.K., Ray, A.K. (2003). Supplementation of an isolated fish gut bacterium, Bacillus circulans, in formulated diets for rohu, Labeo rohita fingerlings. Israeli Journal of Aquaculture-Bamidgeh. 55: 13-21.

Giri, S.S., Sukumaran, V., Oviya, M. (2013). Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and and disease resistance of tropical freshwater fish, Labeo rohita. Fish and Shellfish Immunology. 34(2): 660-666.

Gupta, A., Gupta, P., Dhawan, A. (2014). Dietary supplementation of probiotics affects growth, immune response and disease resistance of Cyprinus carpio fry. Fish and Shellfish Immunology. 41: 113-119.

Halver, J.E. (1957). Nutrition of salmonoid fishes. III. Water-soluble vitamin requirements of chinook salmon. The Journal of Nutrition. 62: 225-243.

Kumar, P., Kaur, V.I., Tyagi, A., Nayyar, S. (2019). Probiotic Potential of Putative Lactic Acid Bacteria Isolated from the Fish Gut: Immune Modulation in Labeo rohita (Ham.). Journal of Coastal Research. 86(SI): 119-127.

Nash, R.D.M., Valencia, A.H., Geffen, A. (2006). The origin of Fulton’s condition factor-Setting the record straight. Fisheries. 31: 236-238.

Parker, R.B. (1974). Probiotics the other half of the antibiotic story. Animal Nutrition and Health. 29: 4-8.

Shome, R., Shome, B.R., Mazumdar, Y., Das, A., Kumar, A. (2005). Abdominal dropsy disease in major carps of Meghalaya: isolation and characterization of Aeromonas hydrophila. Current Science of India. 88: 25.

Sinha, A., Pandey, P.K. (2013). Probiotic effect of a live bacterial isolate in nutrition of an Indian major carp, rohu (Labeo rohita). Indian Journal of Animal Research. 47 (6) : 509-514.

Totewad, N.D. and Gyananath, G. (2021). Effect of Probiotic Enterococcus gallinarum N3 Supplemented Feed on Growth Performance of Freshwater Fish Cyprinus Carpio. Indian Journal of Animal Research. DOI: 10.18805/ag.D-5322.

Valiallahi, J., Pourabasali, M., Janalizadeh, E., Biucio, A. (2018). Use of Lactobacillus for improved growth, and and enhanced biochemical, hematological and digestive enzyme activity in fishes, (Cyprinus carpio L.) at Mazandaran Iran. North American Journal of Aquaculture. 80 (2): 206-215.

Wang, Y.M. and Wang, Y.G. (2008). Advance in the mechanisms and application of microecologics in aquaculture. Progress in Veterinary Medicine. 29: 72-75.

Zhang, J., Zhang, X., Zhang, L., Zhao, Y., Niu, C., Yang, Z., Li, S. (2014). Potential probiotic characterization of Lactobacillus plantarum strains isolated from Inner Mongolia “Hurood” cheese. Journal of Microbiology and Biotechnology. 24(2): 225-235.

Disclaimer :

The Asian Journal of Dairy and Food Research (AJDFR) follows a disclaimer policy that outlines the responsibilities of the journal, authors, reviewers, and readers. The policy is designed to ensure that all published content is accurate and meets ethical standards, and to protect the journal from any legal liability.

The AJDFR does not guarantee the accuracy, completeness, or reliability of the content published in the journal. The opinions expressed in published articles are those of the authors and do not necessarily reflect the views of the journal or its editorial board. The journal does not accept any responsibility for any errors or omissions in the content published in the journal.

Authors are responsible for ensuring that their work is original, accurate, and meets ethical standards. They are also responsible for obtaining permission to use copyrighted material and for providing appropriate attribution.

Reviewers are responsible for ensuring that the content of articles is accurate, original, and meets ethical standards. They are also responsible for maintaining confidentiality and disclosing any conflicts of interest.

Readers are responsible for evaluating the content of articles and for making their own judgments about the accuracy and reliability of the information presented. They are also responsible for complying with copyright laws and ethical standards when using the content of articles.

AJDFR is committed to ensuring that all published content meets ethical standards and that all parties involved in the publishing process understand their responsibilities. The disclaimer policy is designed to protect the journal from any legal liability and to ensure that readers can rely on the accuracy and reliability of the content published in the journal.

Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Asian Journal of Dairy and Food Research

Full Research Article

Efficacy of Probiotic Bacteria (Lactobacillus plantarum) Supplementation in the Diet on Growth and Nutritional Composition of Cirrhinus mrigala Fingerlings

ABSTRACT

KEYWORDS

REFERENCES

Reviewed By

In this Article

APC

Publish With US

Become a Reviewer/Member

Open Access

Products and Services

Support and Policies

Editorial Board