Appraisal of various physico-chemical characteristic and detection of synthetic color in saffron (Crocus sativus)

DOI: 10.18805/ajdfr.DR-1329    | Article Id: DR-1329 | Page : 304-309
Citation :- Appraisal of various physico-chemical characteristic and detection of synthetic color in saffron (Crocus sativus).Asian Journal of Dairy and Food Research.2018.(37):304-309
K.J. Patel and V.R. Boghra kinpatel11@gmail.com
Address : Department of Dairy Chemistry, S.M.C College of Dairy Science, Anand Agricultural University, Anand-388 001, Gujarat, India.
Submitted Date : 25-11-2017
Accepted Date : 21-09-2018


The saffron contained on an average 12.88 per cent moisture and volatile matter, 119.77 (E) coloring strength, 55.85 per cent (on dry matter basis) aqueous extract, 6.56 per cent floral waste, 2.47 per cent (on dry basis) nitrogen, 4.57 per cent total ash and 0.57 per cent acid insoluble ash, 4.63 per cent crude fibre and 4.83 per cent total oil content respectively. None of the sample of saffron exceeded the extraneous matter content of 1 per cent (maximum limit under PFA), so far analyzed. The extracted material from pure saffron in 80 per cent ethanol was subjected to thin layer chromatography resolved in 3 major distinct and 3 minor yellow orange spots. While adulterated samples gave more than 6 spots with different colors.


Characteristics of saffron Crocus sativus Saffron Saffron adulteration Synthetic colors in saffron Thin layer chromatography.


  1. Alonso GL, Salinas MR. and Saez JR. (1998) Crocin as coloring in the food industry. Recent Research Developments in Agric. and Fd. Chem. 2: 141-154.
  2. BIS 1996. IS: 5453 (Part I and II). Specification for saffron and methods of analysis.
  3. Budhiraja KL (1942) Kashmir saffron with methods for testing its purity. J. Indian Chem. Soc. Edu. 5: 135.
  4. Bureau of Indian Standards, New Delhi.
  5. Carmona M, Carrion ME, Zalacain A and Alonso GL (2004) Detection of adulterated saffron through UV- Vis spectral analysis. J. Fd. Sci. Tech. 41: 451-455. 
  6. Francesco, L and Di-Francesco, L. (1990). Saffron. [Edoizioni L’ InformatoreAgrariozVerona, Italy]. p. 38.
  7. Ingram JS (1996) Saffron (crocus sativus L.) Tropical sci. 2: 227-232.
  8. ISO, 1993. ISO 3632-1, Saffron- Specifications, second revision. International Organization of Standardization. Geneva, Switzerland.
  9. Kaul MK (1997) In: Medicinal plants of Kashmir and Ladakh Temperate and Cold Arid Himalaya. Indus Publishing Co., New Delhi. 112. 
  10. Kirtikar KR ,Basu BD and ICS (1975) Crocus sativus. In: Indian Medicinal Plants, 2nd ed. MIS Bishen Singh Mahendra Pal Singh, Dehra Dun. 4: 2462-2463. 
  11. Laboratory Manual (2005). Methods of Analysis of Foods (Spices and Condiments). Directorate General of Health Services. Ministry of Health and Family Welfare. Government of India, New Delhi. pp-14.
  12. Li ,N, Lin G, Kwan YW, and Min ZD (1999) Simultaneous quantification of five major biologically active ingredients of saffron by high-performance liquid chromatography. J. Chromatography A. 849: 349–355.
  13. Mehta BM, Borkhatriya VN, Boghra VR (2002) Saffron- The coloring and flavouring agent in dairy and food industry. J. Medicinal and aromatic plant Sci. 24: 1038-1049.
  14. Ortega H, Pereda-Miranda R, Rivero´n-Negrete, L Herna´ndez JM, Mede´cigo-Rý´os M, Castillo-Villanueva A (2004) Chemical composition of saffron (Crocus sativus L.) from four countries. Acta Horticulturae .650: 321–326.
  15. PFA. (2008). Prevention of Food Adulteration Rules, Commodity index with short notes. International Law Book Co., New Delhi. p. 176. 
  16. Pruthi JS (1976) Spices and condiments. National Book trust, New Delhi, India. P.221-226.
  17. Sastri BN (1953) In: The Wealth of India, Industrial products, part III: D-E. Council of Scientific and Industrial Research, New Delhi. p 67.
  18. Singh R, Kumar K. And Sagwal RC (1997) Research gaps in scientific cultivation of saffron (Crocus sativus L.) in Kashmir. Agric. Sci. Digest. 17: 161-164. 
  19. Snedecor GW and Cochran WG (1980) Statistical Methods. 6th edition, Oxford and IBH Publishing Co. Pvt., New Delhi.
  20. Sujata, V.; Ravishankar, G. A. and Venkataraman, L. V. (1992). Methods for the analysis of the saffron metabolites crocin, crocetins, picrocrocin and safranal for the determination of the quality of the spice using thin-layer chromatography, high-performance liquid chromatography and gas chromatography. J. Chromatography, 624: 497-502. 

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