Studies on absorption of nutrients using intestinal sacs of rats fed on different diets

DOI: 10.18805/ajdfr.v36i02.7964    | Article Id: DR-1146 | Page : 166-169
Citation :- Studies on absorption of nutrients using intestinal sacs of rats fed on different diets .Asian Journal Of Dairy and Food Research.2017.(36):166-169

D. R. Sood*, Shahina Kalim and Rajat Sood
 

rajatsood123@rediffmail.com
Address :

Department of Biochemistry, CCS Haryana Agricultural University, Hisar-125 004, Haryana, India.

Submitted Date : 4-07-2016
Accepted Date : 12-04-2017

Abstract

Absorption of glucose, sucrose, glutamic acid and methionine was studied using the intestinal everted sac technique. Rats were fed with potato tubers of Kufri Ashoka, Kufri Chipsona-1 and Kufri Chipsona-2 in combination with casein in 1:1 ratio and casein as a control at eight per cent, protein level. Absorption of sucrose, glucose, glutamic acid and methionine was monitored spectrophotometrically and of glucose, sucrose and glutamic acid by 14C radio assay using scintillation counting. In general, glutamic acid showed higher absorption values than glucose, sucrose and methionine by both the techniques. Rats fed on casein diet exhibited maximum absorption of glutamic acid and methionine. Incorporation of 10 ?mole of lithium decreased considerably the absorption of glucose, sucrose, glutamic acid and methionine. Absorption of nutrients differed between casein and casein-potato tubers diets.

Keywords

Amino acids Intestinal absorption Lithium Radio assay Spectrophotometric techniques.

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