Loading...

CALCIUM BINDING AND SOLUBILIZING ABILITY OF HYDROPHOBIC AND HYDROPHILIC CASEINOPHOSPHOPEPTIDES (CPPs)

Article Id: ARCC680 | Page : 16 - 19
Citation :- CALCIUM BINDING AND SOLUBILIZING ABILITY OF HYDROPHOBIC AND HYDROPHILIC CASEINOPHOSPHOPEPTIDES (CPPs).Asian Journal of Dairy and Food Research.2012.(31):16 - 19
A. Nagamani*, Jayashri P. Hiremath and Narasimha Murthy nagudscb@gmail.com
Address : Department of Dairy Chemistry, Dairy Science College, Karnataka Veterinary, Animal & Fisheries Sciences University, Bangalore-560 024

Abstract

Skim milk subjected to acidification by the addition of 10% HCl to separate whole casein at 5% degree of hydrolysis for about 3 hr. Casein hydrolysates produced by neutrase and trypsin yielded 75% of hydrophobic and 83.3% relatively hydrophilic CPPs respectively. The CPPs showed calcium binding ability of 0.40 and 0.45 mg of Ca/mg of the CPPs obtained from hydrophobic and relatively hydrophilic CPPs respectively. With statistical significant difference (P³0.05). The solubilizing ability of CPPs of 11.92 and 11.56 mg of Ca/mg of CPPs were observed at 12mg of Ca/mg of CPPs obtained from hydrophobic and relatively hydrophilic CPPs respectively.

Keywords

Caseinophosphopeptides Calcium binding Calcium Solubilizing
Hydrophobic
Hydrophilic.

References

  1. Mellander, O. (1950). Acta Society Medicine Uppsala., 55:247-255.
  2. Fitz Gerald, R. J. (1998). Int. Dairy J., 8: 451-457.
  3. Holt et al. (1996). Biochem. J., 314:1035-1039.
  4. Mohan Kumar, M.D. (2002). M. Sc. Thesis, submitted to UAS, Bangalore, India.
  5. Dinesha, H.M. (2004). M. Sc. Thesis, submitted to UAS, Bangalore, India.
  6. Vogel, A.I. (1961). UASL Publishing Co., 346-438.
  7. Suma, K.N. (1994). M. Sc. thesis, submitted to UAS, Bangalore, India.
  8. Sato et al. (1991). Biochimica et Biophysica Acta, 107:413-441.
  9. Mc Donagh, D. and Fitz Gerald, R.J. (1998). Int. Dairy J., 8:39-45.
  10. Laemmli, U.K. (1976). Nature., 227:680-685.

Global Footprints