Asian Journal of Dairy and Food Research, volume 41 issue 4 (december 2022) : 377-383

​Application of MALDI-TOF Mass Spectrometry to Identify Lactic Acid Bacteria Isolated from Artisanal Dairy Products in Algeria

M. Bouchibane, Y. Zabouri, A. Cheriguene, A. Kaced, F. Chougrani, D. Ait Saada
1Food Technology and Nutrition Laboratory, Mostaganem University, Algeria.
Cite article:- Bouchibane M., Zabouri Y., Cheriguene A., Kaced A., Chougrani F., Saada Ait D. (2022). ​Application of MALDI-TOF Mass Spectrometry to Identify Lactic Acid Bacteria Isolated from Artisanal Dairy Products in Algeria. Asian Journal of Dairy and Food Research. 41(4): 377-383. doi: 10.18805/ajdfr.DRF-280.
Background: Artisanal dairy products are considered an affordable food source and are highly valued by Algerian consumers.
Methods: In this study during the period from 2019 to 2020, phenotypic identification methods and protein analysis by MALDI-TOF MS were performed to identify lactic acid bacteria isolated from 19 sample and five types of artisanal dairy products collected in different Algerian regions (East, West, North, South). 
Results: A number of 250 strains were isolated, only those that showed the characteristics of lactic acid bacteria were identified by analysing their proteins, The results showed that the strains belong to different species of LAB namely: Lactobacillus fermentum (31.03%), Enterococcus faecium (24.14%), Lactobacillus plantarum (21.55%), Enterococcus faecalis (17.24%), Lactococcus lactis ssp lactis (4.31%), Lactobacillus paracasei and Lactobacillus paraplantarum (0.86%). Identification by MALDI-TOF MS was similar to that provided by phenotypic Characterization. This new technique that has been used is considered as an effective tool for rapid identification of food-associated lactic acid strains and has also been successfully extended to dairy isolates.

  1. Abhyankar, P.S., Gunjal, A.B., Kapadnis, B.P. and Ambade, S.V. (2022). Potential of lactic acid bacteria in plant growth promotion. Bhartiya Krishi Anusandhan Patrika. 36(4): 326-329. anusandhan-patrika.

  2. Angelakis, E., Million, M., Henry, M. and Raoult, D. (2011). Rapid and accurate bacterial identification in probiotics and yoghurts by MALDI-TOF mass spectrometry. Journal of Food Science. 76: 568-572.

  3. Arezki, A.A., Nathalie, D., Ahmed, B. (2019). Identification of indigenous Lactobacillus isolated from artisanal Algeria dairy product by 16S rRNA Gene Sequencing and MALDI-TOF Mass Spectrometry. https:// P: 14.

  4. Benamara, R.N. Gemelas, L., Ibri, K., Moussa-Boudjemaa, B., Demarigny, Y. (2016). Sensory microbiological and physico -chemical characterization of Klila, a traditional cheese made in the south-west of Algeria. African Journal of Microbiology Research. 10: 1728 1738.DOI : 10.5897/AJMR 2016.8264.

  5. Carr, F.J., Chill, D. and Maida, N., (2002). The lactic acid bacteria/: A literature survey. Critical Reviews in Microbiology. 28: 281-370. 

  6. Cherkaoui, A., Hibbs, J., Emonet, S., Tangomo, M., Girard, M., Francois, P., Schrenzel, J. (2010). Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. Journal of Clinical Microbiology. 48: 1169-1175. 

  7. Dahou, A.A., Bekaa, A.A. and Homrani, A. (2021). Identification of a Lactococcus lactis Isolated from a Fresh Local Cheese of the Western Algerian Steppe “J’ben of Naama”. Asian Journal of Dairy and Food Research. 40(1): 5. https:// 10.18805/ajdfr. DR-208.

  8. De Man, J.C., Rogosa, M., Sharpe, M.E. (1960). A medium for the cultivation of lactobacilli. Journal of Applied Microbiology. Applied Microbiology. 23: 130-135. j. 1365 2672. 1960.tb00188. x.

  9. Doan, N.T.L., Van Hoorde, K., Cnockaert, M., De Brandt, E., Aerts, M., Le Thanh, B., Vandamme, P. (2012). Validation of MALDI-TOF MS for rapid classification and identification of lactic acid bacteria, with a focus on isolates from traditional fermented foods in Northern Vietnam. Letters in Applied Microbiology. 55: 265 273.

  10. Domingos-Lopes, M.F.P., Stanton, C., Ross, P.R., Dapkevicius, M.L.E., Silva, C.C.G. (2017). Genetic diversity, safety and technological characterization of lactic acid bacteria isolated from artisanal Pico cheese. Food Microbiology. 63: 178 190.

  11. Ferreira, L., Sánchez-Juanes, F., García-Fraile, P., Rivas, R., Mateos, P.F., Martínez-Molina, E., González-Buitrago, J.M., Velázquez, E. (2011). MALDI-TOF Mass Spectrometry Is a Fast and Reliable Platform for Identification and Ecological Studies of Species from Family Rhizobiaceae. PLoS ONE. 6: e20223.

  12. Gantzias, C., Lappa, I.K., Aerts, M., Georgalaki, M., Manolopoulou, E., Papadimitriou, K., De Brandt, E., Tsakalidou, E., Vandamme, P. (2020). MALDI-TOF MS profiling of non- starter lactic acid bacteria from artisanal cheeses of the Greek island of Naxos. International Journal of Food Microbiology. 323: 108586. 

  13. Herbel, S.R., Vahjen, W., Wieler, L.H., Guenther, S. (2013). Timely approaches to identify probiotic species of the genus Lactobacillus. Gut Pathogens. 5: 27. 10.1186/1757-4749-5-27.

  14. Kanak, E.K. and Yilmaz, S.Ö. (2019). Maldi-tof mass spectrometry for the identification and detection of antimicrobial activity of lactic acid bacteria isolated from local cheeses. Food Science and Technology. 39: 462 469. 

  15. López-Dýìaz, T.M., Alonso, C., Román, C., Garcýìa-López, M.L. and Moreno, B. (2000). Lactic acid bacteria isolated from a hand-made blue cheese. Food Microbiology. 17(1): 23 32.

  16. Mathara, J.M., Schillinger, U., Kutima, P.M., Mbugua, S.K., Holzapfel, W.H. (2004). Isolation, identification and characterisation of the dominant microorganisms of kule naoto: The Maasai traditional fermented milk in Kenya. International Journal of Food Microbiology. 94: 269 278.

  17. Meghoufel, N., Homrani, A., Nemmiche, S., Benkrizi, N., Elamine, D., Zbalah, R. (2017). A brief identification of lactic acid bacteria isolated from Algerian goat’s Jben by MALDI- TOF MS. 2230 9799.

  18. Nacef, M., Chevalier, M., Chollet, S., Drider, D., Flahaut, C. (2017). MALDI-TOF  mass spectrometry for the identification of lactic acid bacteria isolated from a French cheese: The Maroilles. International Journal of Food Microbiology. 247: 2-8. 

  19. Patil, P., Wadehra, A., Munjal, K. and Behare, P. (2015). Isolation of exopolysaccharides producing lactic acid bacteria from dairy products. Asian Journal of Dairy and Food Research. 34(4).

  20. Samelis, J., Maurogenakis, F. and Metaxopoulos, J. (1994). Characterisation of lactic acid bacteria isolated from naturally fermented Greek dry salami. International Journal of Food Microbiology. 23: 179 196. 

  21. Sánchez-Juanes, F., Teixeira-Martín, V., González-Buitrago, J.M., Velázquez, E., Flores-Félix, J.D. (2020). Identification of Species and Subspecies of Lactic Acid Bacteria Present in Spanish Cheeses Type “Torta” by MALDI-TOF MS and pheS gene Analyses. Microorganisms. 8: 301.

  22. Seng, P., Drancourt, M., Gouriet, F., La Scola, B., Fournier, P.E., Rolain, J.M.,  Raoult, D. (2009). Ongoing revolution in bacteriology/: Routine identification of  bacteria by matrix- assisted laser desorption ionization time-of-flight mass spectrometry. Clinical Infectious Diseases: An Official Publication of the Infectious  Diseases Society of America. 49: 543 551. 

  23. Soro-Yao, A.A., Schumann, P., Thonart, P., Djè, K.M., Pukall, R. (2014). The use of MALDI-TOF mass spectrometry, ribotyping and phenotypic tests to identify lactic acid bacteria from fermented cereal foods in abidjan (Côte d’Ivoire). The Open Microbiology Journal. 8: 78-86. 

  24. Tanigawa, K., Kawabata, H., Watanabe, K. (2010). Identification and typing of Lactococcus lactis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Applied and Environmental Microbiology. 76: 4055 4062. 

  25. Terzaghi, B.E., Sandine, W.E. (1975). Improved Medium for Lactic Streptococci and their Bacteriophages. Applied Microbiology. 29: 807-81.

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