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Comparison of exopolysaccharide production by indigenous Leuconostoc mesenteroides strains in whey medium

DOI: 10.5958/0976-0563.2015.00002.0    | Article Id: DR-931 | Page : 8-12
Citation :- Comparison of exopolysaccharide production by indigenous Leuconostoc mesenteroides strains in whey medium .Asian Journal of Dairy and Food Research.2015.(34):8-12
Vaibhao Lule*, Rameshwar Singh, Pradip Behare and Sudhir Kumar Tomar vaibhao_only@rediffmail.com
Address : Dairy Microbiology Division, ICAR-National Dairy Research Institute-Karnal, 132001, India.

Abstract

Two Leuconostoc mesenteroides strains NCDC 744 and NCDC 745 were evaluated for exopolysaccharides (EPS) production in whey and MRS medium. Paneer whey medium supplemented with 10% sucrose, 0.1 % yeast extract and 0.1 % K2HPO4 was found to be proferred medium for EPS production. Among two strains L. mesenteroides NCDC 744 produced significantly higher EPS (12.7 ± 0.24 gm/L) as compared to NCDC 745 (10.51 ± 0.18 gm/L). The EPS powder obtained from NCDC 744 after oven drying was white, crystalline, water soluble and contained 89.56 ± 0.37 % of total carbohydrate and 3.49 ± 0.14 % protein. Scanning Electron Micrograph (SEM) study revealed that the EPS from NCDC 744 was porous, compact and crystalline in appearance.

Keywords

Exopolysaccharides Leuconostoc mesenteroides MRS Paneer whey SEM.

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