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Molecular Appreciation of the Native Bacterial Flora of Raw Milk in the North-Western Region of Algeria

DOI: 10.18805/ajdfr.DR-214    | Article Id: DR-214 | Page : 152-156
Citation :- Molecular Appreciation of the Native Bacterial Flora of Raw Milk in the North-Western Region of Algeria.Asian Journal of Dairy and Food Research.2021.(40):152-156
M. Medjahed, A.A. Dahou, N. Desmasures, A. Homrani  mostefa.medjahed@univ-mosta.dz
Address : Laboratory of Sciences and Technics of Animal Production, Abdelhamid Ibn Badis University, Mostaganem-Algeria.
Submitted Date : 7-12-2020
Accepted Date : 15-03-2021

Abstract

Background: The appreciation of microbial milk communities, in order to promote useful flora and inhibit pathogenic one, has become one of the key factors in controlling the quality of cheeses.
Methods: In this context, an approach to assess the bacterial flora of raw cow’s milk, was tested in 05 wilayas in north-western Algeria, on the one hand by a preliminary count of total flora and a phenotypic characterization based on morphological criteria and on the other hand by a genotypic identification of selected isolates, by the PCR method and sequencing of the gene encoding for the 16S ARNr.
Result: The results of molecular analysis obtained from 72 isolates allowed the identification of 12 bacterial species with a dominance of the lactic microflora, 37% Lactococcus and 29% Enterococcus. This milk-native lactic microflora has become the main vector that preserves the distinctiveness of dairy products, including local cheese.

Keywords

Appreciation Genotypic identification Lactic microflora Microbial milk communities

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