Chief EditorHarjinder Singh
Print ISSN 0971-4456
Online ISSN 0976-0563
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Full Research Article
Effect of Maturity Stages and Ripening Process on the Nutritive and β-carotene of Mango [Mangifera indica (L.) var. Manjeera]
Methods: Materials utilized for the study were mangoes harvested at two maturity stages i.e. 7-9°brix and 9-11°brix and processed by three treatments methods viz, control, 100 ppm and 150 ppm ethylene treatment followed by 12 days storage. The samples were withdrawn periodically for analysis on 4th, 8th and 12th day of the storage. Nutritive composition and β-carotene analysis for the mangoes were done using the standard methods.
Result: According to the findings of the proximate analysis, the ash content was greater in the control on the 12th and 4th days, respectively, in the 9-11°brix TSS and 7-9°brix TSS. Mangoes harvested at 7-9°brix TSS had the maximum protein content on the fourth day, followed by mangoes treated with 150 ppm ethylene on the twelfth day. In all the treatments, the maximum levels of crude fiber and crude fat were found on day 12th and 8th, respectively, in mangoes harvested at 7-9°brix TSS and 9-11°brix TSS. On the fourth day, mangoes treated with 100 ppm ethylene and harvested at 9-11°brix TSS had the highest carbohydrate content. On the eighth day, all treatments had the most energy. 7-9°brix TSS harvested mangoes showed good nutrient content in control samples, showing that artificial ripening treatments had a substantial impact on the nutrient composition, according to the data.
Mango is a member of the genus Mangifera and the family Anacardiaceae. Mangifera is a genus that has many species that produce palatable fruit. Mangifera indica, the most popular Mangifera species, is native to India and Myanmar (Bally, 2006). One of the most significant tropical fruits in the world, the mango is now grown for commercial purposes in more than 87 countries (Tharanathan et al., 2006) and is currently rated fifth among the major fruit crops in terms of global production (Ravani and Joshi, 2013).
According to epidemiological research, eating fruits and vegetables helps reduce lung cancer (Yong et al., 1997), neurological illnesses (Joseph et al., 1999) and cardiovascular diseases (Ye and Song, 2008). Contrarily, fiber is known to be essential for regular gastrointestinal function and high intakes of dietary fiber are also advised to reduce the risk of cardiovascular illnesses and coronary heart disease (Slavin, 2013), diabetes (Hopping et al., 2010) and to maintain appropriate body weight (Slavin, 2008).
MATERIALS AND METHODS
Mangoes were harvested at two maturation stages (7-9°brix TSS and 9-11°brix TSS) at the Fruit Research Station, SKLTSHU, Sangareddy. Sixty mature, green, undamaged and healthy fruits of the Manjeera cultivar were gathered. After harvest, the fruits were left to dry for an hour before having their skins cleaned with a towel and being washed with a mild, neutral detergent (Sandovit - 0.5 ml/litre). After drying in the shade, the fruits were brought for an ethylene treatment. At the Fruit Research Station, SKLTSHU, Sangareddy, two ethylene ripening treatments (100 ppm and 150 ppm ethylene dosages) were performed. Fruits were removed from each treatment on days 4, 8 and 12 to be evaluated for their nutritional makeup and β-carotene concentration. Fig 1 contains a flow chart showing the various mango research treatments that were applied.
Moisture: (IS: 1155-1968 reaffirmed 2010)
5 g of dried sample was placed in dried and weighed porcelain dish was kept in electric oven at 105±1°C for 2 hours.
Ash: (IS: 1155-1968 reaffirmed 2010)
5 g of dehydrated mango pulp was dried for 1 hour and then kept in muffle furnace between 550 to 600°C to finish ashing. The weight of grey ash was recorded.
Protein: (AOAC 992.23; 32.2.02.)-generic combustion method; (Leco FP-528 nitrogen analyzer)
0.2 g of EDTA and dehydrated mango pulp and kept in aluminium foil cups. Blank of 0.5 g was entered to calibrate instrument. To determine the percentage of protein, sample id and weights were entered and added one at a time to the crucibles.
Crude fiber (AOAC 962.09-2007)
1 g sample was kept in weighed empty fiber bag and a blank fiber bag and weighed, bags were filled with glass spacers. Bags were placed in the sample carousel’s and placed axially on hot plate and Fibertherm programme was initiated. Once programme was finished fiber bags were dried for 2 hours at 102°C and then weighed. After digestion and drying, the weight of the crucible and fiber bags alongwith blank fiber bag. Crucibles were then heated to 600°C in a muffle furnace. After burning, the weight of the crucible+blank fiber bag was taken.
Crude fat (AOAC 922.06-2006)
Dehydrated mango pulp was measured and wrapped in filter paper, kept in thimbles and then fitted in fat extraction beaker. N-hexane was added to beaker and placed into slots in soxtherm. The sample portion containing thimble was submerged in the boiling solvent. The sample portion was further extracted in the second phase by a continuous flow of condensed solvent for one hour and 20 minutes while the thimble was raised above the solvent. Condensation was used to recover the solvent after it had evaporated. After drying, the resultant crude fat residue was quantified. To get rid of moisture, extraction cups were dried at 102°C±2°C in a hot air oven for 30 minutes. The defatted sample (F) was weighed to the nearest 0.1 mg after cooling in a desiccator (F).
Determination of total carbohydrates (IS 1656:2007 Reaffirmed 2012)
After calculating the percentage of moisture, total protein, total fat and total ash, total carbs were determined as follows. Total carbohydrates, including sucrose dextrose and dextrin, maltose or lactose, percent by mass= 100-(A+B+C+D)
A= Percent by mass of moisture.
B= Percent by mass of total protein.
C= Percent by mass of fat.
D= Percent by mass of total ash.
Estimation of energy content by Atwater general factor system method
Energy content was estimated by multiplying protein, fat and carbohydrate values obtained from analysis by 4, 9 and 4 respectively.
RESULTS AND DISCUSSION
Results for moisture content
The data (Fig 2) show that control mangoes harvested at 7-9°brix TSS had a moisture content that increased significantly (p<0.05) up to day 12. The moisture content of mangoes treated with 100 ppm and 150 ppm decreased substantially (p<0.05) from day 4 to day 8 and then dramatically (p<0.05) increased till twelfth day.
The moisture level in the control group of mangoes collected at 9-11°brix TSS fell somewhat over the course of day 4 to day 8, before considerably rising (p<0.05) till day 12. In mangoes treated with 100 ppm, moisture content increased significantly (p<0.05) from day 4 to day 12, whereas in mangoes treated with 150 ppm, there was a slight decrease in moisture content from day 4 to day 8, before declining significantly (p<0.05) to day 12. While Brito and Narain (2002) noted a drop in moisture content in sapota fruit, Mahmood et al. (2013) discovered a considerable increase in moisture content in cherry fruit during the various ripening stages. However, according to Opara et al. (2012), the moisture content of cherry fruits fluctuates between being lower and higher during various stages of ripening.
Results for ash content
The data show that there are different amounts of ash in the 7-9°brix TSS harvested mangoes for each of the three treatments. In 9-11°brix TSS harvested mangoes ash content in control and100ppm treated mangoes decreased significantly (p<0.05) from 4th day to 8th day of storage and then there was a significant (p<0.05) increase from 8th day to 12th day of storage, whereas in 150 ppm ethylene treated mangoes there was a significant (p<0.05) increase in the ash content from 4th day to 8th day of storage and there was no increase in ash content from 8th day to 12th day (Fig 3).
While Sharma et al. (2014) discovered that the ash content of wood apples decreased and increased over the ripening process, Mahmood et al. (2013) discovered that the ash level in cherry fruits decreased from the unripe stage to the semi-ripe stage and decreased significantly to the fully-ripened state. The ash content of the native pawpaw variety significantly altered from the unripe to the semi-ripe stage and then again from the semi-ripe to fully ripe stage, according to a study by Wurochekke et al. (2013). The changing capacity for absorption of the minerals at different phases of growth, according to Adeyemi and Oladiji (2009), may be the cause of differences in ash.
Results for protein content
The protein content of control mangoes harvested at 7-9°Brix TSS and 9-11°brix TSS decreased from the fourth to the eighth day of storage then increased from the eighth to the twelfth day, according to the protein estimation results (Fig 4).
The protein content of the pawpaw types Kapoho Solo and Solo Sunrise decreased from the unripe to the semi-ripe stage and then increased up to the ripe stage of the fruit, according to Wurochekke et al. (2013). In a study on cherry fruit, Mahmood et al. 2013 found that as the fruit ripens, the protein concentration increases. The amount of protein in blackberry fruit reduces as it ripens, according to Tosun et al. (2008).
Results for crude fat
According to the data (Fig 5), the crude fat content of both the 7-9°brix and 9-11°brix TSS harvested mangoes was maximum on the eighth day in all treatments. Our findings concur with those of Opara et al. (2012), who found that the fat content of the Isabella cultivar of tomato increased initially before declining.
Results for crude fiber
According to the crude fiber analysis (Fig 6), the amount of crude fiber in 7-9°brix TSS harvested mangoes increased from the fourth to the eighth to the twelfth day of storage in control mangoes and in mangoes treated with 150 ppm ethylene. In 9-11°brix TSS harvested mangoes, there was a significant (p<0.05) increase in fiber content from 4th day to 12th day of storage in control as well as 100 ppm and 150 ppm treated mangoes.
According to research by Brito and Narain (2002), Wurochekke et al. (2013) and Mahmood et al. (2013), the crude fiber content of cherry, sapota and pawpaw fruit reduced as the fruit ripened. However, the findings of our analysis are at odds with those of other studies because we discovered a consistent rise in the amount of crude fiber in manjeera mangoes.
Results for carbohydrate content
According to the data (Fig 7), the maximum carbohydrate content was discovered on the eighth day in mangoes harvested with TSS at 7-9°brix and on the fourth day in control and 100 ppm-treated mangoes harvested at 9-11°brix. In 9-11°brix TSS harvested mangoes, there was a significant (p<0.05) decrease in carbohydrate content from the fourth to the eighth day in all treatments. According to a study by Wurochekke et al. (2013), the amount of carbohydrates in the kapoho solo and solo dawn varieties of pawpaw increased during the ripe stage after first increasing during the semi-ripe stage. After fruit is harvested, cellulase and pectinmethylestrease activity gradually rises, reaching a peak at the edible stage.
Results for β-carotene content
According to the observations in Fig 8, the carotene content of the mangoes harvested at 7-9°brix and 9-11°brix increased significantly (p<0.05) from day 4 to day 12 in all treatment groups (Control, 100 ppm, 150 ppm). According to a study by Azad et al. (2009) on four mango varieties, mangoes’ β-carotene content rises the longer they are stored. Studies by Abushita et al. (1997)., Nour et al. (2014) in mangoes and Hdider et al. (2013) in tomatoes reported an increase in β carotene concentration with increased ripening, which is in agreement with the current finding in mangoes.
As per the results obtained (Table 2) highest energy was found during the 8th day of ripening in all the treatments.
Conflict of Interest
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