Foam Fractionation Technology for Enrichment and Recovery of Cheese Whey Proteins

DOI: 10.18805/ajdfr.DR-182    | Article Id: DR-182 | Page : 187-194
Citation :- Foam Fractionation Technology for Enrichment and Recovery of Cheese Whey Proteins.Asian Journal Of Dairy and Food Research.2020.(39):187-194
Venkateswarlu Sunkesula, Anil Kommineni, Chenchaiah Marella, K. Muthukumarappan, Lloyd E. Metzger sunkesula.venkat@gmail.com
Address : Department of Dairy and Food Science, South Dakota State University, Brookings, SD 57007. South Dakota.
Submitted Date : 4-06-2020
Accepted Date : 22-08-2020

Abstract

Background: Foam fractionation technology works on the adsorptive bubble separation principle. This technique involves adsorption of the surface-active substances on to a gas-liquid interface and separation of these components from the liquid along with bubbles as foam. The foam separation technology has been successfully utilized in the recovery of proteins from solutions containing either a single protein or binary mixtures. To develop a foam fractionation technology for selective enrichment and recovery of whey proteins, it is essential to investigate the effect of different feed and process variables that affect the foam fractionation process. The aim of the current study was to investigate the effect of two important feed variables, such as pH and initial protein concentration on recovery and enrichment of total whey proteins as well as α-lactalbumin and β-lactoglobulin.
Methods: All the experiments were conducted in Agriculture and biosystems engineering lab and Alfred Dairy Science lab at South Dakota State University, Brookings, South Dakota during 2011-2013. The experiments used four levels of initial protein concentration and five levels of feed pH. Yield and enrichment ratios were determined for total whey proteins, α-Lactalbumin (α-La) and β-Lactoglobulin (β-Lg). 
Result: Whey protein yields ranged from 51.58 to 90.92%, while the enrichment ratios were between 1.2 to 5. The yield of α-La varied from 59 to 94% and the highest enrichment ratio of 8.45 was obtained with the treatment combination of initial protein concentration of 109 mg/L and pH of 5.1. Selective enrichment of α-La over β-Lg was observed at a pH of 4.65 with α-La to β-Lg ratio of 0.49. These findings will be helpful in selective enrichment and recovery of valuable proteins from Cheddar cheese whey using the foam fractionation process.

Keywords

Cheese whey Enrichment ratio Foam fractionation Whey proteins α-Lactalbumin β-Lactoglobulin

References

  1. Anand, K. and Damodaran, S. (1995). Kinetics of adsorption of lysozyme and bovine serum albumin at the air–water interface from a binary mixture. Journal of Colloid and Interface Science. 176: 63-73.
  2. Angela, G. (2004). Paper presented at the 11th Annual workshop for Dairy Economists and Policy Analysts, Washington, D C.
  3. Arunkumar, A. and Etzel, M. (2018). Fractionation of Glycomacropeptide from Whey Using Positively Charged Ultrafiltration Membranes. Foods. 7: 166. 
  4. Backleh-Sohrt, M., Ekici, P., Leupold, G. and Parlar, H. (2005). Efficiency of foam fractionation for the enrichment of nonpolar compounds from aqueous extracts of plant materials. Journal of Natural Products. 68: 1386-1389.
  5. Bhattacharya, P., Ghosal, S. and Sen, K. (1991). Effect of physicochemical parameters on the separation of proteins from human placental extract by using a continuous foam fractionating column. Separation Science and Technology. 26: 1279-1293.
  6. Brown, A., Kaul, A. and Varley, J. (1999a). Continuous foaming for protein recovery: Part I. Recovery of â casein. Biotechnology and Bioengineering. 62: 278-290.
  7. Brown, A., Kaul, A. and Varley, J. (1999b). Continuous foaming for protein recovery: Part II. Selective recovery of proteins from binary mixtures. Biotechnology and Bioengineering. 62: 291-300. 
  8. Brown, L., Narsimhan, G. and Wankat, P. (1990). Foam fractionation of globular proteins. Biotechnology and Bioengineering. 36: 947-959.
  9. Casal, E., Montilla, A., Moreno, F. J., Olano, A. and Corzo, N. (2006). Use of chitosan for selective removal of â-lactoglobulin from whey. Journal of Dairy Science. 89: 1384-1389.
  10. Chai, J., Loha, V., Prokop, A. and Tanner, R.D. (1998). Effect of bubble velocity and pH step changes on the foam fractionation of sporamin. Journal of Agricultural and Food Chemistry. 46: 2868-2872. 
  11. Chandrasekar, V., Gabriela, J.S., Kannan, K. and Sangamithra, A. (2015). Effect of foaming agent concentration and drying temperature on physiochemical and antimicrobial properties of foam mat dried powder. Asian Journal of Dairy and Food Research. 34: 39-43.
  12. Changade, S.P., Bhandari, P.N., Chapake, J.S. and Shinde, N.W. (2009). Foaming in food systems. Journal of Dairying, Foods and Home Sciences. 28: 26-30.
  13. Dickinson, E. and Matsumura, Y. (1994). Proteins at liquid interfaces: role of the molten globule state. Colloids and Surfaces B: Biointerfaces. 3: 1-17. 
  14. Ekici, P., Backleh-Sohrt, M. and Parlar, H. (2005). High efficiency enrichment of total and single whey proteins by pH controlled foam fractionation. International Journal of Food Sciences and Nutrition. 56: 223-229. 
  15. Hammam, A.R. (2019). Technological, applications and characteristics of edible films and coatings: A review. SN Applied Sciences. 1: 632.
  16. Harper, W.J. (1999). Biological properties of whey components: A review: American Dairy Products Institute.
  17. Harwalkar, V. and Kalab, M. (1985). Thermal denaturation and aggregation of b-lactoglobulin in solution electron microscopic study. Milchwissenschaft. 40(2): 65-68. 
  18. Horwitz, W. and Latimer Junior, G. (2005). Official methods of analysis of the Association of Analytical Chemists International. Gaythersburg: AOAC International. 
  19. Huang, D., Wu, Z. L., Liu, W., Hu, N. and Li, H. Z. (2016). A novel process intensification approach of recovering creatine from its wastewater by batch foam fractionation. Chemical Engineering and Processing: Process Intensification. 104: 13-21. 
  20. Hunter, J.R., Carbonell, R.G. and Kilpatrick, P.K. (1991). Coadsorption and exchange of lysozyme/â-casein mixtures at the air/water interface. Journal of Colloid and Interface Science. 143: 37-53. 
  21. Johansen, A.G., Vegarud, G.E. and Skeie, S., 2002. Seasonal and regional variation in the composition of whey from Norwegian Cheddar-type and Dutch-type cheeses. International Dairy Journal. 12: 621-629.
  22. Keller, R., Orsel, R. and Hamer, R. (1997). Competitive adsorption behaviour of wheat flour components and emulsifiers at an air–water interface. Journal of Cereal Science. 25: 175-183. 
  23. Li, R., Chen, X. e., Chang, Y., Zhang, L., Zhang, Y., Zhu, Y. and Wang, T. (2017). Increase of bubble size playing a critical role in foam-induced protein aggregation: Aggregation of BSA in foam fractionation. Chemical Engineering Science. 174: 387-395. 
  24. Li, R., Fu, N., Wu, Z., Wang, Y. and Wang, Y. (2015). Protein aggregation in foam fractionation of bovine serum albumin: effect of protein concentration. Biochemical Engineering Journal. 103: 234-241. 
  25. Li, R., Fu, N., Wu, Z., Wang, Y., Liu, W. and Wang, Y. (2016). Enhancing protein self-association at the gas–liquid interface for foam fractionation of bovine serum albumin from its highly diluted solution. Chemical Engineering Research and Design. 109: 638-646. 
  26. Liu, L., Zhang, W., Yu, X., Lei, L. and Liu, H. (2018). Process optimization for foam separation of yak whey protein by response surface methodology. Separation Science and Technology. 53: 2327–2337. 
  27. Lockwood, C.E., Jay, M. and Bummer, P.M. (2000). Foam fractionation of binary mixtures of lysozyme and albumin. Journal of Pharmaceutical Sciences. 89: 693-704. 
  28. Mate, J. and Krochta, J.M. (1994). â Lactoglobulin separation from whey protein isolate on a large scale. Journal of Food Science. 59: 1111-1114. 
  29. Matouq, M. (2008). Investigation of the bubble foam separation technique to extract protein from whey. Am J Appl Sci. 5: 468-472. 
  30. Montero, G.A., Kirschner, T.F. and Tanner, R.D. (1993). Bubble and foam concentration of cellulase. Applied Biochemistry and Biotechnology. 39: 467-475. 
  31. Morr, C. and Ha, E. (1993). Whey protein concentrates and isolates: processing and functional properties. Critical Reviews in Food Science and Nutrition. 33: 431-476. 
  32. Mukhopadhyay, G., Khanam, J. and Nanda, A. (2010). Protein removal from whey waste by foam fractionation in a batch process. Separation Science and Technology. 45: 1331-1339. 
  33. Muller, A., Chaufer, B., Merin, U. and Daufin, G. (2003). Purification of $\alpha $-lactalbumin from a prepurified acid whey: Ultrafiltration or precipitation. Le Lait. 83: 439-451. 
  34. OECD/FAO. (2017). OECD-FAO Agricultural Outlook2017-2026. OECD Publishing. doi:https://doi.org/10.1787/agr_outlook -2017-en
  35. Phillips, L., Hawks, S. and German, J. (1995). Structural Characteristics and Foaming Properties of â-Lactoglobulin: Effects of Shear Rate and Temperature. Journal of Agricultural and Food Chemistry. 43: 613-619. 
  36. Pinfold, T. (1970). Adsorptive bubble separation methods. Separation Science. 5: 379-384. 
  37. Prazeres, A.R., Carvalho, F. and Rivas, J. (2012). Cheese whey management: A review. Journal of Environmental Management. 110: 48-68. 
  38. Prokop, A. and Tanner, R. D. (1993). Foam fractionation of proteins: Potential for separations from dilute starch suspensions. Starch Stärke. 45: 150-154. 
  39. Sarkar, P., Bhattacharya, P., Mukherjea, R. and Mukherjea, M. (1987). Isolation and purification of protease from human placenta by foam fractionation. Biotechnology and Bioengineering. 29: 934-940. 
  40. Shea, A., Crofcheck, C., Payne, F. and Xiong, Y. (2009). Foam fractionation of á lactalbumin and â lactoglobulin from a whey solution. Asia Pacific Journal of Chemical Engineering. 4: 191-203. 
  41. Shimizu, M., Saito, M. and Yamauchi, K. (1985). Emulsifying and structural properties of â-lactoglobulin at different pHs. Agricultural and Biological Chemistry. 49: 189-194. 
  42. Singh, A.K., Nath, N. and Arora, S. (2006). Composition and thermal behaviour of whey protein preparations under acidic conditions. Journal of Dairying, Foods and Home Sciences. 25: 8-14.
  43. Stevenson, P. and Li, X. (2014). Foam Fractionation: Principles and Process Design: CRC press.
  44. Stowers, C.C., Makarov, V., Walker, A., Edwards, R.A. and Tanner, R.D. (2009). Effect of air flow rate on the foam fractionation of a mixture of egg white and egg yolk. Asia Pacific Journal of Chemical Engineering. 4: 180-183. 
  45. Strohmaier, W. (2004). Chromatographic fractionation of whey proteins. Bulletin-International Dairy Federation. 389: 29-35. 
  46. Suzuki, A., Yasuhara, K., Seki, H. and Maruyama, H. (2002). Selective foam separation of binary protein solution by SDS complexation method. Journal of Colloid and Interface Science. 253: 402-408. 
  47. Tian, S., Wu, Z., Liu, W., Zhang, M., Lv, Y., Xu, Y. and Zhao, Y. (2018). Effective recovery of casein from its highly diluted solution by using a technology of foam fractionation coupled with isoelectric precipitation. Journal of Food Engineering. 216: 72-80. 
  48. Uraizee, F. and Narsimhan, G. (1996). Effects of kinetics of adsorption and coalescence on continuous foam concentration of proteins: comparison of experimental results with model predictions. Biotechnology and Bioengineering. 51: 384-398. 
  49. USDA. (2017). Dairy: world markets and trade. United States Department of Agriculture, Foreign Agricultural Service. doi:https://apps.fas.usda.gov/psdonline/circulars/dairy.pdf
  50. Verheul, M., Pedersen, J. S., Roefs, S. P. and de Kruif, K. G. (1999). Association behavior of native â lactoglobulin. Biopolymers: Original Research on Biomolecules. 49: 11-20.

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