Loading...

Association of SSCP Polymorphisms of HSP70 Gene with Physiological, Production and Reproduction Performance in Sahiwal and Crossbred Cows

DOI: 10.18805/ajdfr.DR-1796    | Article Id: DR-1796 | Page : 150-155
Citation :- Association of SSCP Polymorphisms of HSP70 Gene with Physiological, Production and Reproduction Performance in Sahiwal and Crossbred Cows.Asian Journal of Dairy and Food Research.2022.(41):150-155
J. Sai Prasanna, S.T. Viroji Rao, M. Gnana Prakash, Suresh Rathod, P. Kalyani, B. Rajith Reddy drsaiprasannareddy@gmail.com
Address : Department of Animal Genetics and Breeding, College of Veterinary Science, P.V. Narsimha Rao Telangana Veterinary University, Rajendranagar-500 030, Hyderabad, Telangana, India.
Submitted Date : 11-08-2021
Accepted Date : 27-09-2021

Abstract

Background: Cellular tolerance to heat stress is mediated by heat shock proteins (HSPs). The HSPs act as molecular chaperones and are transcribed in response to stress. Among different families of these proteins, HSP70 is considered to be related to the development of temperature tolerance. Unraveling polymorphism in heat shock protein genes could be a step towards the identification of genetic markers for selecting heat-tolerant cattle. 
Methods: The present study was carried out in Sahiwal (n=50) and Crossbred cows (n=50) with the objective to identify polymorphisms in HSP70 gene. Two fragments (295 and 220 bp) of HSP70 gene were subjected to Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique. Statistical analysis was performed to study the association of each SSCP genotype on physiological, production and reproduction traits in Sahiwal and crossbred cows using the univariate GLM model of SPSS 25.
Result: The PCR-SSCP of 295 bp fragment of HSP70 gene revealed two genotypes AA and AB in Sahiwal cows and two genotypes AA and AC in crossbred cows. The association analysis revealed that genotype AA had higher peak milk yield in Sahiwal cows while the same genotype had higher total lactation milk yield, lower service period and calving interval in crossbred cows. The 220 bp fragment was found to be monomorphic in both Sahiwal and crossbred cows. 

Keywords

Crossbred cows Heat stress HSP70 Polymorphisms Sahiwal

References

  1. Archana, P.R., Aleena, J., Pragna, P., Vidya, M.K., Abdul Niyas, P.A., Bagath, M., Krishnan, G., Manimaran, A., Beena, V., Kurien, E.K., Sejian, V. and Bhatta, R. (2017). Role of heat shock proteins in livestock adaptation to heat stress. Journal of Dairy, Veterinary and Animal Research. 5(1): 13-19.
  2. Bassam, B.J. and Gresshoff, P.M. (2007). Silver staining DNA in polyacrylamide gels. Nature Protocols. 2(11): 2649-54. 
  3. Benezra, M.V. (1954). A new index for measuring the adaptability of cattle to tropical conditions. Journal of Animal Science. 13: 1015. 
  4. Berman, A.J. (2005). Estimates of heat stress relief needs for Holstein dairy cows. Journal of Animal Science. 836: 1377-84. 
  5. Bhat, S., Kumar, P., Kashyap, N., Deshmukh, B., Dige, M.S., Bhushan, B., Chauhan, A., Kumar, A. and Singh, G. (2016). Effect of heat shock protein 70 polymorphism on thermotolerance in Tharparkar cattle. Veterinary World. 92: 113-17.
  6. Boonkum, W., Misztal, I., Duangjinda, M., Pattarajinda, V., Tumwasorn, S. and Sanpote, J. (2011). Genetic effects of heat stress on milk yield of Thai Holstein crossbreds. Journal of Dairy Science. 94: 487-92.
  7. Cai, Y., Liu, Q., Xing, G., Zhou, L., Yang, Y., Zhang, L., Li, J. and Wang, G. (2005). Polymorphism of the promoter region of HSP70 Gene and its relationship with the expression of HSP70 mRNA, HSF1mRNA, Bcl-2mrna and Bax-AMrna in lymphocytes in peripheral blood of heat shocked dairy cows. Asian-Australasian Journal of Animal Sciences. 18(5): 734-40.
  8. Chaiyabutr, N., Chanpongsang, S. and Suadsong, S. (2008). Effects of evaporative cooling on the regulation of body water and milk production in crossbred Holstein cattle in a tropical environment. International Journal of Biometerology. 52: 575-85.
  9. Collier, R.J., Dahl, G.E. and Van Baale, M.J. (2006). Major advances with environmental effects on dairy cattle. Journal of Dairy Science. 89: 1244-53.
  10. Dalcin, V., Fischer, V., Daltro, D., Alfonzo, M., Priscila, E., Marcelo, S., Giovani, K., Marcos, S. and Concepta, M. (2016). Physiological parameters for thermal stress in dairy cattle. Revista Brasileira de Zootecnia. 45: 458-65.
  11. Deb, R., Sajjanar, B. and Singh, U. (2013). Promoter variants at AP2 box region of Hsp701 affect thermal stress response and milk production traits in Frieswal cross bred cattle. Gene. 9: 37.
  12. Gade, N., Mahapatra, R.K., Sonawane, A., Singh, V.K., Doreswamy, R. and Saini, M. (2010). Molecular characterization of heat shock protein 70-1 gene of goat (Capra hircus). Molecular Biology International. Article ID 108429.
  13. Green, M.R., Sambrook, J. (2012). Molecular Cloning: A Laboratory Manual. New York, USA: Cold Spring Harbor.
  14. Hassan, F., Nawaz, A., Rehman, M.S., Ali, M.A., Dilshad, S.M.R. and Yang, C. (2019). Prospects of HSP70 as a genetic marker for thermo-tolerance and immuno-modulation in animals under climate change scenario. Animal Nutrition. 54: 340-50. 
  15. Herrero, M. and Thornton, P.K. (2013). Livestock and global change: Emerging issues for sustainable food systems. Proceedings of National Academy of Sciences of the United States of America. 110(52): 20878-81.
  16. Kerekoppa, R., Rao, A., Basavaraju, M., Geetha, G., Krishnamurthy, L., Narasimha, L., Das, D. and Mukund, K. (2015). Molecular characterization of the HSPA1A gene by single-strand conformation polymorphism and sequence analysis in Holstein-Friesian crossbred and Deoni cattle raised in India. Turkish Journal of Veterinary and Animal Sciences. 39: 128-33.
  17. Kumar, R., Gupta, I.D., Verma, A., Verma, N., Nishant and Vineeth, M.R. (2015). Single nucleotide polymorphisms in Heat Shock Protein (HSP) 90AA1 gene and their association with heat tolerance traits in Sahiwal cows. Indian Journal of Animal Research. 51: 64-69.
  18. Li, Q., Han, J., Du, F., Ju, Z., Huang, Z., Wang, J., Li, R., Wang, C. and Zhong, J. (2011). Novel SNPs in HSP70A1A gene and the association of polymorphisms with thermo tolerance traits and tissue specific expression in Chinese Holstein cattle. Molecular Biology Reports. 38: 2657-63.
  19. Naqvi, S. and Sejian, V. (2011). Global Climate Change: Role of Livestock. Asian Journal of Agricultural Sciences. 3(1): 19-25.
  20. Polsky, L. and Keyserlingk, M.A.G. von. (2017). Invited review: Effects of heat stress on dairy cattle welfare. Journal of Dairy Science. 100(11): 8645-57. 
  21. Rojas-Downing, M.M., Nejadhashemi, A.P., Harrigan, T. and Woznicki, S.A. (2017). Climate change and livestock: Impacts, adaptation and mitigation. Climate Risk Management. 16: 145-63.
  22. Rosenkrans, C., Banks, A., Reiter, S. and Looper, M. (2010). Calving traits of crossbred Brahman cows are associated with heat shock protein 70 genetic polymorphisms. Animal Reproduction Science. 119(3-4): 178-82. 
  23. Sailo, L., Gupta, I.D., Verma, A., Chaudhari, M.V., Das, R., Upadhyay, R.C. and Goswami, J. (2015). Single Nucleotide Polymorphisms in HSP90AB1 gene and its association with thermo tolerance in Jersey crossbred cows. Animal Science Reporter. 9: 43-49.
  24. Scholtz, M., Maiwashe, A., Neser, F., Theunissen, A., Olivier, W., Mokolo-bate, M and Hendriks, J. (2013). Livestock breeding for sustainability to mitigate global warming, with the emphasis on developing countries. South African Journal of Animal Science. 43(3): 269-81.
  25. Shergojry, S.A., Ramesha, K.P., Mir, N.A. and Aarif, O. (2014). Nucleotide sequence polymorphism within Exon 8 of Heat Shock Protein HSP 90AA1 gene and its association with milk production traits in Deoni cows. Indian Journal of Animal Research. 48(5): 408-12.
  26. Singh, C.V. (2016). Cross-breeding in Cattle for Milk Production: Achievements, Challenges and Opportunities in India-A Review. Advances in Dairy Research. 4(3): 158.
  27. Sodhi, M., Mukesh, M., Kishore, A., Mishra, B.P., Kataria, R.S. and Joshi, B.K. (2013). Novel polymorphisms in UTR and coding region of inducible heat shock protein 70.1 gene in tropically adapted Indian zebu cattle (Bos indicus) and riverine buffalo (Bubalus bubalis). Gene. 527(2): 606-15. 
  28. Wilson, T. and Crandall, C. (2010). Effect of thermal stress on cardiac function. Exercise and Sport Sciences Reviews. 39(1): 12-17.

Global Footprints