Asian Journal of Dairy and Food Research, volume 40 issue 4 (december 2021) : 384-387

Identification of β Casein Genotypes in Indian Gir and Crossbred Exotic Cows from Mumbai Dairy Farms

P.H. Paradkar, V.M. Loke, C.G. Godse, R.A. Vaidya, A.D.B. Vaidya
1Kasturba Health Society and Medical Research Centre, 17, Sthanakwasi Jain Aradhana Dham, Mumbai-400 056, Maharashtra, India.
Cite article:- Paradkar P.H., Loke V.M., Godse C.G., Vaidya R.A., Vaidya A.D.B. (2021). Identification of β Casein Genotypes in Indian Gir and Crossbred Exotic Cows from Mumbai Dairy Farms. Asian Journal of Dairy and Food Research. 40(4): 384-387. doi: 10.18805/ajdfr.DR-1659.
Background: Collective evidence of polymorphic β-casein and associated health problems has led to the concern about milk consumption and cow breeding policies worldwide. This association has also engrossed the interest of dairy scientist and industry in evaluation of β casein genotype distribution. With increasing proportion of exotic and crossbred cows in India it is worth while to screen cattle for A1A2 β casein and enhance indigenous cow breeds.
Methods: The present study intended to identify β casein genotypes in pure Indian Gir cows and crossbred Holstein and jersey cows from three local dairy farms. We analysed β casein genotypes by PCR-RFLP method in total 95 cows during the period of 2017-2019. 
Result: All the indigenous Gir cows had fixed A2 allele whereas crossbred Jersey and Holstein Frisian both had A1A2 as the most common genotype (frequency: 0.473 and 0.6 respectively) followed by A2A2 (Frequency 0.368 and 0.333 respectively) and A1A1 (Frequency 0.158 and 0.066 respectively). The results show that in this study group Gir, a native Indian breed has fixed A2 β casein variant whereas crossbred Jersey and Holstein Frisian have A1A2 as a most common genotype. Screening of cattle for â casein genotypes is vital to monitor the frequency of A1 beta casein in native Indian cow breeds.

  1. 18th Livestock census, (2007). All India Report, Ministry of Agriculture Department of Animal Husbandry, Dairying and Fisheries Krishi Bhawan, New Delhi. 

  2. 19th Livestock census. (2012) All India Report, Ministry of Agriculture Department of Animal Husbandry, Dairying and Fisheries Krishi Bhawan, New Delhi, 2014.

  3. 20th Livestock census. (2019). All India Report, Ministry of Agriculture Department of Animal Husbandry, Dairying and Fisheries Krishi Bhawan, New Delhi..

  4. Caroli, A.M., Chessa, S. and Erhardt, G.J. (2009). Milk protein polymorphisms in cattle: Effect on animal breeding and human nutrition. Journal of Dairy Science 92(11): 5335-52.

  5. Cieślińska, A., Fiedorowicz, E., Zwierzchowski, G., Kordulewska, N., Jarmołowska, B. and Kostyra, E. (2019). Genetic polymorphism of β-casein gene in polish red cattle- Preliminary study of A1 and A2 frequency in genetic conservation herd. Animals. 9(6): 377. 

  6. De, S., Singh, R.K., Gupta, P.K., Palia, S. and Butchaiah, G. (2000). Genotyping of dairy animals using DNA from milk somatic cells. Indian Journal Animal Sciences. 70: 944e946.

  7. Elliott, R.B., Harris, D.P., Hill, J.P., Bibby, N.J. and Wasmuth, H.E. (1999). Type I (insulin dependent) diabetes mellitus and cow milk: casein variant consumption. Diabetologia. 42:  292-96.

  8. Farrell, H.M.,  Jimenez-Flores, R., Bleck, G.T., Brown, E.M., Butler, J.E., Creamer, L.K., Hicks, C.L., Hollar, C. M., Ng-Kwai- Hang, K.F. and Swaisgood, H. E. (2004). Nomenclature of the proteins of cows’ milk-sixth revision. Journal of Dairy Science. 87(6): 1641-74.

  9. Ganguly, I., Gaur, G.K., Singh, U., Kumar, S. and Mann, S. (2013). Beta-casein (CSN2) polymorphism in Ongole (Indian zebu) and Frieswal (HF × Sahiwal crossbred) cattle. Indian Journal of Biotechnology. 12: 195-98.

  10. Godse, C.S., Paradkar, P.H., Loke, V.M., Udipi, S.A., Vaidya, R.A. and Vaidya, A.D.B. (2017). Cow’s Milk: Nutritional relevance beyond its intolerance and allergies. The Indian Practitioner 70(4): 29-34.

  11. Kaminski, S., Cieslinska, A. and Kostyra, E. (2007). Polymorphism of bovine β-casein and its potential effect on human health. Journal of Applied Genetics. 48(3): 189-98.

  12. Kaminski, S., Ruœæ, A. and Cieœliñska, A. (2006). A note on frequency of A1 and A2 variants of bovine beta-casein locus in Polish Holstein bulls. Journal of Animal Feed Science 15: 195-98.

  13. Kumar, P., Choudhary, V., Bhattacharya, T.K., Bhushan, B. and Sharma, A. (2005). PCR-RFLP based genotyping of cattle using DNA extracted from hair samples. Indian Journal of Biotechnology 4: 287-89.

  14. Kumar, S., Singh, R.V., Chauhan, A., Kumar, A. and Yadav, J.S. (2020). Analysis of beta-casein gene (CSN2) polymorphism in Tharparkar and Frieswal cattle. Indian Journal of Animal Research. 54(1): 1-5.

  15. Kumar, S., Singh, R.V. and Chauhan, A. (2019). Molecular characterization of A1/A2 Beta-casein Alleles in Vrindavani crossbred and Sahiwal cattle. Indian Journal of Animal Research, 53 (2): 151-155

  16. Lamb, M.M., Miller, M., Seifert, J.A., Frederiksen, B., Kroehl, M., Rewers, M. and Norris, J.M. (2015). The effect of childhood cow’s milk intake and HLA-DR genotype on risk of islet autoimmunity and Type 1 Diabetes: The diabetes autoimmunity study in the young (DAISY). Pediatric Diabetes 16(1): 31-38.

  17. Lien, S., Aleström, P., Klungland, H. and Rogne, S. (1992). Detection of multiple β-casein (CASB) alleles by amplification created restriction sites (ACRS). Animal Genetics. 23: 333-38.

  18. McLachlan, C.N. (2001). B-casein A1, ischaemic heart disease mortality, and other illnesses. Medical Hypotheses. 56: 262-72.

  19. Mishra, B.P., Mukesh, M., Prakash, B., Sodhi, M., Kapila, R., Kishore, A., Kataria, R.R., Joshi, B. K., Bhasin, V., Rasool, T.J. and Bujarbaruah, K.M. (2009). Status of milk protein, b-casein variants among Indian milch animals. Indian Journal of Animal Sciences. 79(7): 722-25.

  20. Ng-Kwai-Hang, K.F. and Grosclaude, F. (2002). Genetic polymorphism of milk proteins. Advanced Dairy Chemistry, Chapter 16, pp. 737-814. (Eds) Fox PF and McSweeney PLH, Kluwer Academic/Plenum Publishers, New York. 

  21. Pal, S., Woodford, K., Kukuljan, S. and Ho, S. (2015). Milk Intolerance, Β-Casein and Lactose. Nutrients. 7(9): 7285- 97.

  22. Raja, A., Rajendran, R. and Ganapathi, P. (2021). Detection of A1 and A2 Alleles at Beta-casein Locus in Bargur and Umblachery (Indian Zebu) Cattle Breeds by Allelespecific PCR. Indian Journal of Animal Research. DOI: 10.18805/ IJAR.B-4273.

  23. Ramesha, K.P., Rao, A., Basavaraju, M., Alex, R., Kataktalware, M.A., Jeyakumar, S. and Varalakshmi, S. (2016). Genetic variants of Beta-Casein in Cattle and Buffalo breeding bulls in Karnataka state of India. Indian Journal of Biotechnology. 15: 178-81.

  24. Rupasinghe, R.K., Shanjayan, N., Lokugalappatti, L.G.S. and Wickramasinghe, S. (2020). Genetic Variants of β-casein Gene in Indigenous and Exotic Dairy Cattle Breeds in Sri Lanka. Asian Journal of Dairy and Food Research. 39(3): 217-220

  25. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.

  26. Shashank, C.G., Puri, R.K., Gandhi, G., Kaur, T. and Kushwaha, M.K. (2018). A1 and A2 beta casein: Twin faces of milk. Journal of Pharmacognosy and Phytochemistry. 7(4): 221-224.

  27. Sodhi, M., Mukesh, M., Kataria, R.S., Mishra, B.P. and Joshi, B.K. (2012). Milk proteins and human health: A1/A2 milk hypothesis. Indian Journal of Endocrinology and Metabolism. 6(5): 856. 

  28. Trivedi, M.S., Shaha, J.S., Al-Mughairya, S., Hodgsona, N.W., Simmsa, B., Trooskensb, G.A., Criekingeb, W.V. and Detha, R.C. (2014). Food-derived opioid peptides inhibit cysteine uptake with redox and epigenetic consequences. Journal of Nutritional Biochemistry. 25(10): 1011-18.

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