Characterization of Natural Lactic Flora in a Soft Cheese “Camembert of the Tessala” Made From Thermised Milk of Local Breed Cow “Brown of the Atlas”

DOI: 10.18805/ajdfr.DR-158    | Article Id: DR-158 | Page : 30-34
Citation :- Characterization of Natural Lactic Flora in a Soft Cheese “Camembert of the Tessala” Made From Thermised Milk of Local Breed Cow “Brown of the Atlas”.Asian Journal Of Dairy and Food Research.2020.(39):30-34
A.A. Dahou, A.M.A. Bekada, M. Medjahed, H.Tahlaiti, S.N. Rechidi, A. Homrani
Address : Laboratory of Sciences and Technics of Animal Production, Abdelhamid Ibn Badis University, Mostaganem, Algeria.
Submitted Date : 11-01-2020
Accepted Date : 6-03-2020


Microbial communities play an important role in the maturation of cheese and determine to a large degree its taste quality. The typicality and the sensory richness of Tessala camembert diversity is preserved by this microflora. In the present study, we tried after isolation and purification to characterize genotypically lactic microflora of this cheese at the end of maturation of the transformation from thermized milk of local breed cow. The bacterial DNA from twenty-two purified lactic culture was established by an amplification of ribosomal DNA 16S by specific universal primers of prokaryotes with strains reference of each isolated bacterial species used as positive controls. The results show the characteristic of the Tessala camembert by the diversity of its original lactic flora dominated by lactococci, enterococci, lactobacilli, pediococci and leuconostocs, preserved by the initial thermization of milk used to maintain the technologically-interested dairy microbial ecosystem.


Camembert of the Tessala Characterization Lactic microflora


  1. Aissaoui, Z.O. (2006). Making and characterizing a traditional Algerian cheese “Bouhezza». Ph.D. Thesis in Food Science from the Institute of Nutrition, Food and Food Technology INATAA Constantine, Algeria. p. 27-107.
  2. Casalta, E. (2003).Scientific Bases of the Quality of the Venaco, traditional cheese from raw milk. Development of Specific Selected Ferments. Theses, University of Burgundy. Dijon, France.p.56-82.
  3. Delbes, C., Monnet, C., Irlinger, F. (2015). Microbial communities in the service of the quality of the cheeses. Agricultural Innovations. 44 (1): 69-86.
  4. Depouilly, A., Dufrene, F., Beuvier, E., Berthier, F. (2004). Genotypic British of the dynamics of the lactic acid bacterial population of County cheese, milk. 84(1): 155-167.
  5. Dubernet, S., Desmasures, N., Gueguen, M. (2008). Diversity and dynamics of lactobacilli populations during ripening of RDO Camembert cheese. Canadian Journal of Microbiology. 54(3): 218-228.
  6. Fricker, M., Skanseng, B., Rudi, K., Stassi, B., Ehling– Schulz, M. (2011). Shift from farm to dairy tank milk microbiota revealed by a polyphasic approach is independent from geographical origin. International Journal of Food Microbiology. 145(1): 24-30.
  7. Irlinger, F., Layec, S., Helinck, S., Dugat-Bony, E. (2015).Cheese rind microbial communities: diversity, composition and origin. FEMS Microbiol. Lett. 362(1-11): 10-19
  8. Jadhav, B.S., Kulkarni, M.B., Chavan, K.D. (2008). Effect of lactation order and stage of lactation on physical properties of milk. J. Dairying, Foods and H.S. (ARCC Journals), 27(3/4):168-174. 
  9. Jany, J.L. and Barber, G. (2008). Culture-independent methods for identifying microbial communities in cheese. Food Microbiol.25(7): 839-848.
  10. Leclercq-Perlat, M.N. (2011). Cheese: Camembert, Brie and related varieties. In Editor in chief: John. WF, editor. Encyclopedia of Dairy Sciences (Second Edition). San Diego: Academic Press. pp. 773-782. 
  11. Manzo, N., Santini, A., Pizzolongo, F., Aiello, A., Marrazzo, A., Meca, G., Durazzo, A., Lucarini, M. , Romano, R. (2019). Influence of ripening on chemical characteristics of a traditional Italian cheese: Provolone del Monaco. Sustainability. 11(9): 2520-2528.
  12. Monnet, C., Landaud, S., Bonnarme, P., Swennen, D. (2015). Growth and adaptation of microorganisms on the cheese surface. FEMS Microbial. Lett. 362(1): 1-9.
  13. Montel, M.C., Bouton, Y., Parguel, P. (2012). Ecosystems milks and cheeses milk issues for their mastery. Renc. Rech. 
  14. Ruminants, F - 15000 Aurillac. INRA. 19(1): 545-560.O’Sullivan, D.J., Cotter, P.D., O’Sullivan, O., Goblin, L., Mcsweeney, P.L.H., Sheehan, J.J. (2015). Temporal and spatial differences in microbial composition during the manufacture of a continental-type cheese. Applied and Environmental Microbiology. 81(7) : 2525-2533.
  15. Quigley, L., O’Sullivan, O., Beresford, T.P., Ross, P., Fitzgerald, G.F., Cotter, P.D. (2011). Molecular approaches to analysis the microbial composition of raw milk and raw milk cheese. International Journal of Food and Microbiology. 150(2-3): 81-94.
  16. Reats, D., Offek, M., Minoz, D., Halpern, M. (2011). Molecular analysis of bacterial communities in raw cow milk and the impact of refrigeration on its structure and dynamics, Food. Microb. 28 (3): 465-471.
  17. Sakore, D.B, Dhole, P.T., Chavan, K.D., Pawar, B.K. (2007). Role and viability of probiotic cultures in cow milk DAHI. J. Dairying, Foods and H.S. 26(2): 63-68. 
  18. Shekkar, C., Motina, E., Kumar, S. (2010). Microbiological quality of raw milk and its public health significance. J. Dairying, Foods and H.S. 29(1): 15-18.

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