Study on Physico-Chemical and Microbial Quality of Raw Milk Collected From Different Places of Assi Region in Varanasi City, Varanasi  

DOI: 10.18805/ajdfr.DR-1498    | Article Id: DR-1498 | Page : 1-9
Citation :- Study on Physico-Chemical and Microbial Quality of Raw Milk Collected From Different Places of Assi Region in Varanasi City, Varanasi .Asian Journal of Dairy and Food Research.2020.(39):1-9
Pankaj Kumar Gupta, Dinesh Chandra Rai, Vinod Kumar Paswan, Rajendra Panta, Ashok Kumar Yadav rajendrapanta3@gmail.com
Address : Department of Animal Husbandry and Dairying, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221 005, Uttar Pradesh, India.
Submitted Date : 23-11-2019
Accepted Date : 4-03-2020


The present study was conducted to evaluate the physico-chemical characteristics including Adulteration and Microbiological quality of cow raw milk collected from four different places of ASSI region in Varanasi. Samples were analysed to know the chemical composition, the results showed that the statistically average percentage of Moisture (87.46), Fat (3.87), Protein (3.15), Lactose (4.42), Ash (.712), pH (6.43) and acidity (0.147). The keeping quality of milk was evaluated by Methylene Blue Reduction Test (MBRT). This phenomenon testified that milk sample 1 is fair quality and remained sample were found good and excellent. The microbiological conclusion confirmed the presence (less or more) of microbial load in all the raw milk samples. The highest level of microbial quality in standard plate count (SPC) was 19.1×106 cfu/ml. in sample 1 and in logarithm value is 7.28 cfu/ml at the same time, the highest coliform bacteria 2.3×102 in logarithm value is 2.36 was found in the sample 2. The adulterations in raw milk were checked by the standard procedure. In cow’s raw milk the different mixed adulterant were found in two samples contaminated with detergent and pulverized soap. Besides different hazardous chemical adulterant, raw milk from sample 1 was detected with presence of hydrogen peroxide and sample 2 was contaminated formalin whereas urea was present in sample 2 and 4. Milk adulteration is a global concern and social problem. Increased demand, growth in competition in dairy industry and financial gain makes some producers to adulterate the milk thereby decreasing milk quality. 


Adulterated milk Coliform MBRT Microbial quality Raw milk SPC


  1. A.O.A.C. (1990). Official Methods of Analysis. Association of Official Analytical Chemists, The Association: Arlington, VA, Vol. II, 15th ed. Sec.985.29. 
  2. A.O.A.C., (2005). FDA Bacteriological Analytical Manual, 18th Ed., Washington, DC.
  3. American Public Health Association (APHA), (1998). Standard methods for the examination of water and waste water; 20th edition, Washington. 
  4. Banda, P.T. (2010). Dairy processing module MAV 411. SADC-    University of Zimbabwe regional postgraduate programme in dairy science and technology, Harare, Zimbabwe. Pp 1. 
  5. Bates., C.J. Prentice., A. (1996). Vitamins, minerals and essential trace elements. In: Drugs and Human Lactation, 2nd Edition, (Bennett, P.ed)
  6. Bhattacharjee., I., Chatterjee., S. N., Chatterjee., S.K. and Chandra, G., (2006). Microbiological examination of milk in Tarakeswar, India with special reference to coliforms. Afr. J. Biotechnol. 5(15): 1383-1385. 
  7. Bongard., R. Merker., M.P. Shundo., R. Okamoto., Y. Roerig., D.L. Linehan., J.H. Dawson., C.A. (1995). Reduction of thiazine dyes by bovine pulmonary arterial endothelial cells in culture. American Journal of Physiology. 269(1 Pt 1): L78-84.
  8. Buchbinder L., Baris Y., Aldd E., Reynolds E., Dilon E., Pessin V., Pincas L. and Strauss A., (1951). Publ. Hlth. Rep. 66: 327. 
  9. Davie.s, J.E Freed., V. H. and Whittemore., F.W. (1986). An agro medical approach to pesticide management. Coral Gables, FL: University of Miami School of Medicine. 
  10. Dehinenet., G. Mekonnen., H. Ashenafi., M. and Emmanuelle., G. (2013). Determinants of raw milk quality under a smallholder production system in selected areas of Amhara and Oromia National Regional States, Ethiopia, Agriculture and Biology journal of North America. ISSN Print: 2151-    7517. 
  11. FAO. (1999). Extension Services for Quality Milk Production. Proceedings of an International Workshop in conjunction with the East-West-Forum of the Federal Ministry for Food, Agriculture and Forestry, Germany. 
  12. FAO. Food outlook (2012). global market analysis. Rome, FAO. 
  13. Food Safety and Standards Authority of India (FSSAI), 2011. 
  14. Haftu., K. S. and Degnet H. M. (2018). Determination of Adulteration and Chemical Composition of Raw Milk Sold in Hossana Town, South Ethiopia, Journal of Dairy and Veterinary Sciences. 6(5): 555699.
  15. Harrington., P.J., Archer., J.P., Davis., D.R., Croft. and Varma., J.K. (2002). Outbreak of Campylobacter jejuni infections associated with drinking unpasteurized milk through a cow-leasing program-Wisconsin. 51: 548-549. 
  16. Hemme., T. Otte., J. (2010). Status of and prospects for smallholder milk production-a global perspective Rome, FAO.
  17. Hertzler S.R., Huynh B.C.L., Savaiano D.A. How much lactose is low lactose? J. Am. Diet. Assoc. 1996; 96: 243–246. doi: 10.1016/S0002-8223(96)00074-0. [PubMed] [CrossRef] [Google Scholar] 
  18. Hossain, M.B. and Dev, S.R. (2013). Physiochemical characteristics of various raw milk samples in a selected dairy plant of Bangladesh. International Journal of Engineering and Applied Sciences. 1(3): 91-96.
  19. Impert O, Katafias A, Kita P, Mills A, Pietkiewicz-Graczyk A and Wrzeszcz G ‘Kinetics and mechanism of a fast leuco methylene blue oxidation by copper (II)-halide species in acidicaqueous media. Dalton Transaction, 1994; 5: 348-35.
  20. Kamthania., M. Saxena., J. Saxena., K. Sharma., D.K. (2014). Methods of Detection andRemedial Measures. Int J Engg Tech Res. 1: 15–20.
  21. Kandpal., S.D, Srivastava., A.K, Negi., K.S. (2012). Estimation of quality of raw milk (open and branded) by milk adulteration testing kit. Indian Journal of Community Health. 3: 188-192. 
  22. Kim., H.J., Hardy., G. Novak., J.P., Ramet and F Weber, (1983). Off-tastes in raw and reconstituted milk. FAO Animal. 11: 198-205. 
  23. Md., A.H.M. Aminul., I., Mohammad, S. Mahmud., A., S.M.A. Uddin. and S., A. (2015). Microbial analysis of raw and pasteurized milk from selected areas of Dinajpur. Bangladesh Asian J. Med. Biol. Res. 1(2): 292-296.
  24. Merker, M.P. Bongard, R.D. Linehan, J.H. Okamoto, Y. Vyprachticky, D. Brantmeier., B.M. Roerig, D.L. and Dawson, C.A. (1997). Pulmonary endothelial thiazine uptake: separation of cell surface reduction from intracellular reoxidation. American. Journal of Physiology. 
  25. Mohammed., A.A.G., Khadir., E.K., Abubakar., M. ,Waleed., A. and Wei., Z. (2013). Evaluation of some physicochemical parameters of three commercial milk products, Pak. J. Food Sci. 23(2): 62-65.
  26. Muhammad, K.I. Altaf, A. Hanif, A.A. Anjum and M.Y. Tipu. (2009). Monitoring of Hygienic Status of Raw Milk Marketed in Lahore City, Pakistan. The Journal of Animal and Plant Sciences. 19(2): 74-77.
  27. National Dairy Development Board (NDDB). O’Connor, C.B. (1995). “Rural Dairy Technology ILRI Training Manual I”, International Livestock Research Institute, Nairobi, Kenya. 
  28. Parekh., T.S. and Subhash., R. (2008). Molecular and bacteriological examination of milk from different milch animals with special reference to Coliforms. Current Research in Bacteriology 1(2): 56 - 63. 
  29. Sharma, RSR. Barui, AK, Ed. (2012). Rapid Methods for Detection of Adulterants in Milk Chemical analysis of value added dairy products and their quality assurance. National Dairy Research Institute.
  30. Singuluri H, Sukumaran MK (2014). Milk Adulteration in Hyderabad, India – A Comparative Study on the Levels of Different Adulterants Present in Milk. J Chromatograph Separat Techniq 5: 212. doi:10.4172/2157-7064.1000212 
  31. Steele, M.L., Mcnab, W.B., Poppe, C. Graffiths, M.W., Chen, S. Degrandis, S.A., Fruhner, L.C., Larkin, C.A., Lynch, J.A. and Odumeru, J.A. (1997). Survey of Ontario bulk tank milk for food borne pathogens. J. Food Prot. 60: 1341-1346.
  32. Teklemichael., T. Ameha., K. , Eyassu., S.(2015). Physico Chemical Properties of Cow Milk Produced and Marketed in Dire Dawa Town. Eastern Ethiopia Food Science and Quality Management , vol 46: 56. 
  33. Yadav, S.P., Paswan, V.K., Singh, P., Bhinchhar, B.K. and Gupta, P.K. (2018). Chemical Composition and Mineral Profile of Concentrate Feeds from Dairy Farms of Varanasi, India. Asian J. Dairy and Food Res. 37 (3): 182-186. 

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