A study on the antimicrobial resistant patterns of Pseudomonas Aeruginosa isolated from raw milk samples in and around Tirupati, Andhra Pradesh.

DOI: 10.18805/ajdfr.v36i02.7951    | Article Id: DR-1196 | Page : 100-105
Citation :- A study on the antimicrobial resistant patterns of Pseudomonas Aeruginosa isolated from raw milk samples in and around Tirupati, Andhra Pradesh. .Asian Journal Of Dairy and Food Research.2017.(36):100-105

C.S.Swetha*, A. Jagadeesh Babu, K. Venkateswara Rao, S. Bharathy, R.A. Supriya and T.Madhava Rao

drswetha.vet@gmail.com
Address :

Department of Veterinary Public Health and Epidemiology, College of Veterinary Science, Tirupati-517 502, Andhra Pradesh, India

Submitted Date : 19-12-2016
Accepted Date : 8-04-2017

Abstract

This study was aimed to detect the prevalence of Pseudomonas aeruginosa in milk (n=125) samples which were collected from local vendors, private dairy farms in and around Tirupati. Pre-enrichment was done by taking 10ml of each sample and inoculated in 90 ml of Tryptone Soya broth and incubated at 370C for 24hrs.  A loopful of culture was taken from broth and streaked on nutrient agar and Cetrimide agar plates and incubated at 370C for 24hrs which were further confirmed by biochemical tests. The nineteen positive samples for P.aeruginosa were further tested for antimicrobial susceptibility which has shown multi drug resistant ranging from four to twelve antimicrobials and Multiple Antibiotic Resistance (MAR) index ranges from 0.33 to 1.The isolates of P.aeruginosain  the present study are highlyresistant to ampicillin, penicillin, and oxacillin (100%) and maximum sensitive to Vancomycin (5.3%) followed by Tetracycline (10.5%).

Keywords

Antimicrobial Resistance Milk Multiple Antibiotic Resistance index (MAR index) Pseudomonas aeruginosa.

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