Biochemical characterization of fruit juice for impact of storage on commercial beverages 

DOI: 10.18805/ajdfr.DR-1182    | Article Id: DR-1182 | Page : 316-321
Citation :- Biochemical characterization of fruit juice for impact of storage oncommercial beverages.Asian Journal Of Dairy and Food Research.2017.(36):316-321
Address : National Rice Research Institute, Bidyadharpur, Cuttack-753 006, Odisha, India
Submitted Date : 8-11-2016
Accepted Date : 29-09-2017


The study was performed to evaluate the impact of storage time on safety and hygienic quality of commercial fruit beverage on the basis of market survey. Three most popular brands and three least popular brands of fruit beverages like squash and syrup were selected and stored for nine months at room temperature. The samples were subjected to chemical analysis for food colour, preservative (SO2), heavy metals (Pb and Cd), pectin and microbial analysis for bacteria, fungus, yeast and E. coli at bimonthly interval. Lead content in all the beverages and synthetic permitted food colours like sunset yellow 136.5 ppm in squash A and tartazine 133.2 ppm in squash B and 310.9 ppm in Syrup F was found which exceeded the permissible limit. Preservative (SO2) and cadmium content remained within the acceptable limit. Total bacterial and fungal count increased sharply with the advancement of storage particularly from 7th month onwards. Duration of storage had no effect on the colour and heavy metal content of the beverages.


Food colour Fruit beverage Heavy metals Microbial analysis.


  1. Agarwal, G.P. and Hasija, S.K. (1986).Microorganisms in the Laboratory. Print House India Ltd, Lucknow, 155 p.
  2. Alex, L., Suresh, C.P., Kabir, J and Dhua,R.S.(2003). Litchi juice processing and preservation. Advances in plsci. 16: 215-218
  3. Anwar,A., Mahmood, T., Qamar- Ui- Haque.,Khan, M.Z., Kiswar, F., Perveen, R and Ismat S. (2014). Heavy metals in fruit juices in different packing material. Fuuast J. Biol. 4(2): 191-194.
  4. Averbeck M, Schieberle P (2010). Influence of different storage conditions on changes in the key aroma compounds of orange juice reconstituted from concentrate. Eur. Food Res. Technol. 217:1366- 1380
  5. Chahal, G. S. and Saini, S. P. S. 1999. Storability of juice from New Hybrid watermelon variety. Indian Food Packer, 53: 12-17
  6. Deanna M Minich and Jeffrey S Bland (2007). Acid-alkaline balance: role in chronic disease and detoxification. Alternative Therapies 13(4): 62–65.
  7. Dosumu O, Oluwaniyi O, Awolola GV, Okunola MO (2009). Stability studies and mineral concentration of some Nigerian packed fruit juices, concentrate and local beverages. Afr. J. Food Sci. 3(3): 082- 085
  8. FAO (1979).Food and Agricultural Organisation.Mannual of Food Quality Control. 4. Microbiological Analysis FAO, Rome.
  9. Franke AA, Cooney RV, Henning SM and Custer LJ (2005). Bioavailability and antioxidant effects of orange juice components in humans. J Agric Food Chem 53 (13): 5170–8.
  10. Goyle, A and Ojha, P.(1998).Effect of storage on vitamin C, microbial load and sensory attributes of orange juice. J Fd Sci Technol. 35: 346-348
  11. Hussain, S., Siddique, M.1., Parveen N.and N.Z. Parwaz. (1993). Effect of packaging on the quality of fruit juice based drinks. JAPS, 3 (1-2): 15-18.
  12. Indian Council of Medical Research (1990).Manual-Methods of analysis for adulterants and contaminants in foods. (eds. Toteja GS, Mukharjee A, Mittal R and Saxena BN) Eighth edition.Indian Council of Medical Research. New Delhi. 302 p.
  13. Ingram,M.(1949). Behaviour of sulphur dioxide in concentrated orange juice. Fd Res.14: 54-56.
  14. Jannalagadda, P.R., Rao, P., Bhat, R.V., Naidu, A.N and Rao, P.(2004). Type, extent and use of colours in RTE foods prepared in the non-industrial sector – a case study from Hyderabad, India. Int. J Fd Sci Technol. 39:125-131.
  15. Kaushik, V and Nath, N.(1991). Effect of storage on chemical constituents and sensory qualities of unripe mango beverage base. Beverage Fd Wld. 18: 9-11.
  16. Khan, I., Mehmood, Z., Khan, M and Fatima, T. (2016).Analysis and detection of heavy metals present in fruit juices of Lahore. Int. J. Engg. Sci. Computing 6(4): 3536-3539.
  17. Kim, M.H.(2004). Determination of lead and cadmium in wines by graphite furnace atomic absorption spectrometry.Fd addit contam. 21: 154-157.
  18. Landon S (2007). Fruit juice nutrition and health (Review). Food Australia 59 (11): 533-538. 
  19. Mannay S, Shadaksharaswany CM (2005). Foods: Facts and Principles. (2nd ed.). New Age International Ltd. Publishers. New Delhi, India
  20. Mgaya-Kilima, B., Remberg, F.S., Chove, B.EandWicklund, T. (2014).Influence of storage temperature and time on the physicochemical and bioactive properties of roselle-fruit juice blends in plastic bottle. Food Science and Nutrition 2(2): 181–191.
  21. Moore, J.W and Ramamoorthy,S.(1984). Heavy metals in natural waters – Applied Monitoring and Impact Assessment.Heavy Metals in Natural Water. Springer-Verlag, New York Inc., 268 p. 
  22. National Institute of Nutrition. (2004). Report on the field trip to Plachimada in Palakkad District of Kerala. Annual Report 2003-    2004. National Institute of Nutrition. Hyderabad, pp. 86-88
  23. Ranganna, S.(1986).Handbook of Analysis and Quality Control for Fruit and Vegetable Products.Second edition.Tata Mc Graw-Hill Publishing Co., Ltd., New Delhi.1-13 p.
  24. Thakur, N.K., Lal, Kaushal, B.B and Joshi, V.K.(2000). Effect of different conditions of storage on physio-chemical and microbial qualities of debittered kinnow juice concentrate. J Fd Sci Technol. 37: 415-418
  25. Tripathi, M., Khanna, S. K and Das, M. (2007). Surveillance on use of synthetic colors in eatables vis prevention of Food Adulteration Act of India. Food Control 18(3): 211-219. 
  26. Sahar, S., Soltan, A., Manal, M and Shehata, E. M. (2012).The effects of using color foods of children on immunity properties and liver, kidney on Rats. Food Nutrition Sci. 3: 897-904 

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