Asian Journal of Dairy and Food Research, volume 37 issue 2 (june 2018) : 144-148

PCR based detection and Biofilm formation of Salmonella from fresh coriander leaves (Coriandrum sativum)

S. Leonal Rabins, A. Bhattacharya, V.J. Ajay Kumar, C. Vijayan
1Department of Veterinary Public Health and Epidemiology, Rajiv Gandhi Institute of Veterinary Education and Research Kurumbapet, Puducherry – 605 009, India.
Cite article:- Rabins Leonal S., Bhattacharya A., Kumar Ajay V.J., Vijayan C. (2018). PCR based detection and Biofilm formation of Salmonella from fresh coriander leaves (Coriandrum sativum). Asian Journal of Dairy and Food Research. 37(2): 144-148. doi: 10.18805/ajdfr.DR-1158.
The aim of the study was to detect Salmonella from coriander leaves by polymerase chain reaction, determine biofilm formation and antimicrobial resistance profiling of the isolates. Four Salmonella isolates were recovered from the total of 60 samples. The isolates were confirmed by PCR. The confirmed isolates were subjected for biofilm production assay and showed that all Salmonella isolates were able to form biofilm. Biofilm forming isolates were also subjected for antimicrobial resistance profiling. Three isolates were showed sensitivity against norfloxacin (92.5%). One was showing sensitivity against cefotaxime (90%). All the four isolates were showing complete resistant to ciprofloxacin, nalidixicacid, chloramphenical, gentamicin and cephalexin. Clindamycin and ofloxacin were showed sensitivity 20%. The finding of this study shows that coriander leaves are potential host for the transmission of Salmonella to human and animals. The ability of the isolates to form biofilm reveals the potential of the isolates to persist on the green leafy vegetables and the pathogenic status of the isolates as well as ability to resist antimicrobial chemotherapy.
  1. Abadias M, Usall J, Anguera M, Solsona C and Vinas I (2008) Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments. Int. J. Food Microbiol.123: 121-129.
  2. Annous BA, Fratamico PM and Smith JL (2009) Quorum sensing in biofilms: why bacteria behave the way they do. J. Food Sci. 74(1): 1–14.
  3. Berger CN, Sodha SV, Shaw RK, Griffin PM, Pink D, Hand P and Frankel G (2010) Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environ. Microbiol. 12 : 2385-2397
  4. Cliver D O (1997) Food-borne viruses. In: [Doyle MP, Beuchat LR, Montville TJ (eds.)] Food Microbiology: Fundamentals and Frontiers, Washington, DC, American    Society for Microbiology, 437-446
  5. Chia L L, Cheng H C, Chishih C, Yhu C H, Tzou Y L and Jonathan T O (2007) A multiplex polymerase chain reaction method for rapid identification of Citrobacter freundii and Salmonella species, including Salmonella typhi. J. Microbiol. Immunol. Infect. 40: 222-226
  6. Clinical and Laboratory Standard Institute (CLSI) (2006). Performance standards for antimicrobial susceptibility testing; sixteenth informational supplement. Clinical and Laboratory Standards Institute, 7th edition, Wayne, Pa, USA. 2006; M100-S16, 26:.15-130.
  7. Dhanalakshmi G, Prabha R and Jagadheesh kumar D (2015) Prevalance, antibiotic resistance and biofilm producing meat isolates in and around Namakkal, TN, India, Inter. J.Advinterdis Res.2(2):6-10.
  8. Gordon D (2000) Ceftriaxone-resistant Salmonella infection from antibiotic-treated cattle. Gastroenterol. 119: 3
  9. Hood S.K and Zottola E.A (1997) Adherence to stainless steel food-borne microorganisms during growth in model food systems. Inter. J. Food Microbiol. 37:145 -153.
  10. Houdt V.R and Michiels CW (2010) Biofilm formation and the food industry, a focus on the bacterial outer surface. J Appl Microbiol. 109: 1117-31
  11. Igbinosa S.H (2015) Biofilm formation of Salmonella speciesisolated from fresh cabbage and spinach J. Appl. Sci. Environ. Manage. 19(1): 45-50.
  12. Igbinosa I.H, Chigor VN, Igbinosa EO, Obi LC and Okoh AI (2013)Antibiogram, adhesive characteristics, and incidence of class 1 Integron in Aeromonas species isolated from two South African rivers. Bio Med Research Inter.Article, 8.
  13. Joseph B, Otta SK and Karunasagar I (2001) Biofilm formation by Salmonella spp. on food contact surfaces and their sensitivity to sanitizers. Inter. J. Food Microbiol.64: 367-372.
  14. Lertworapreecha M, Sutthimusik S and Tontikapong K (2013) Antimicrobial Resistance in Salmonella enteric Isolated From Pork, Chicken, and Vegetables in Southern    Thailand. Jundishapur J Microbiol. 6 (1):36-41.
  15. Learn-Han L, Yoke-Kqueen C, Shiran MS, Sabrina S, Noor Zaleha AS, Sim JH, Chai-Hoon, K. and Son R (2009) Molecular characterization and antimicrobial resistance profiling of Salmonella enteric subsp. Enteric isolated from ‘Selom’ (Oenanthest olonifera). Inter. Food Res. J. 16:191-202.
  16. Mukherjee A, Cho S, Scheftel J, Jawahir S, Smith K, Diez-Gonzalez F (2006) Soil survival of Escherichia coli O157:H7 acquired by a child from garden soil recently fertilized with cattle manure. J. of App. Microbio. 101(2): 42943
  17. Nillian E, Ching C L, Fung P C, Robin T, Anyi U, Chilek T Z T, Radu S And Nishibuchi M 2011) Simultaneous detection of Salmonella spp., Salmonella enteritidis and Salmonella typhimurium in raw salad vegetables and vegetarian burger patties. Food and Nutrition Sci.2:1077-1081.
  18. Nair A, Balasaravanan T, Malik SVS, Mohan V, Kumar M, Vergis J and Rawool DB (2015) . Isolation and identification of Salmonella from diarrhoeagenic infants and young animals, sewage waste and fresh vegetables, Veterinary World 8(5):669-673.
  19. Parveen S, Taabodi M, Schwarz J G, Oscar T P, Harter-Dennis J and White D G (2007)    Prevalence and antimicrobial resistance of Salmonella recovered from processed poultry J. Food Protect. 70: 2466-2472.
  20. Patel J and Sharma M (2010) Differences in attachment of Salmonella enteric serovars to cabbage and lettuce leaves. Int. J. Food Microbiol. 139:41-47.
  21. Raufu I A, Zongur L, Lawan F A, Bello H S, Adamu M S, Ameh J A and Ambali A G (2014) Prevalence and antimicrobial profiles of Salmonella serovars from vegetables in Maiduguri, North Eastern Nigeria. Sokoto J.Vet.Sci.12:23-28
  22. Rahn K, De Grandis SA, Clarke RC, Curtiss, R and Gyles, CL (1992) Amplification of an invA gene sequence of Salmonalla typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Molecular and Cellular Probes 6: 271-279.
  23. Salleh NA, Rusul G, Hassan Z, Reezal A, Isa SH, Nishibuchi M and Radu S (2003) Incidence of Salmonella spp. in raw vegetables in Selangor, Malaysia. Food Control 14: 475-479.
  24. Schikora A, Garcia AV and Hirt H (2012). Plants as alternative hosts for Salmonella. Trends in Plant Sci. 17: 245-249.
  25. Sivapalasingam S, Friedman CR, Cohen L and Tauxe RV (2004) Fresh produce: a growing cause of outbreaks of foodborne illness in the United States, 1973 through 1997. J. Food Protect.67: 2342-2353.
  26. Stepanovic S, Cirkovic I, Ranin L and Svabic- vlahovic M (2004) Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface. Letters Applied Microbiol. 38: 428-432.
  27. Tang P L, Pui C F, Wong W C, Noorlis A and Son R (2012) Biofilm forming ability and time course study of growth of Salmonella typhi on fresh produce surfaces. Inter. Food Res. J. 19: 71-76.
  28. Veterinary Microbiology and Microbial Diseases (2007) 2nd Edition. Quinn PJ, Markey BK, Leonard FC, Fitzpatrick ES, Fanning S and Hartigen PJ.
  29. White DG, Zhao S, Sudler R, Ayers S, Friedman S, Chen S, McDermott PF, McDermott S, Wagner DD and Meng J (2001) The isolation of antibiotic-resistant Salmonella from retail ground meats. N. Engl. J. Med. 345 :1147-1154.
  30. Wegener HC, Hald T, Wong LD, Madsen M, Korsgaard N, Bager F, Gerner-Smidt P and Molbak K (2003) Salmonella control programs in Denmark. Emerging Infect. Dis. 9: 774-780.
  31. Yoke-Kqueen C, Learn-Han L, Noorzaleha AS, Son R, Sabrina S, Jiun-Horng S and ChaiHoon K (2008) Characterization of multiple antimicrobial resistant Salmonella enteric subsp. Enteric isolated from indigenous vegetables and poultry in Malaysia. Letters Appl. Microbiol. 46: 318-324.
  32. Zahraei Salehi, T., Gharagozlou, M.J., Shams, N., Madadgar, O., Nayeri Fasaei, B. and Yahyaraeyat,R. (2012). Molecular characterization of a SalmonellaTyphimurium isolate from Caspian pony. Iranian Journal of Biotechnology 10: 49-53. 

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