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Asian Journal of Dairy and Food Research, volume 37 issue 2 (june 2018) : 144-148

PCR based detection and Biofilm formation of Salmonella from fresh coriander leaves (Coriandrum sativum)

S. Leonal Rabins, A. Bhattacharya, V.J. Ajay Kumar, C. Vijayan
1Department of Veterinary Public Health and Epidemiology, Rajiv Gandhi Institute of Veterinary Education and Research Kurumbapet, Puducherry – 605 009, India.
Cite article:- Rabins Leonal S., Bhattacharya A., Kumar Ajay V.J., Vijayan C. (2018). PCR based detection and Biofilm formation of Salmonella from fresh coriander leaves (Coriandrum sativum). Asian Journal of Dairy and Food Research. 37(2): 144-148. doi: 10.18805/ajdfr.DR-1158.

ABSTRACT

The aim of the study was to detect Salmonella from coriander leaves by polymerase chain reaction, determine biofilm formation and antimicrobial resistance profiling of the isolates. Four Salmonella isolates were recovered from the total of 60 samples. The isolates were confirmed by PCR. The confirmed isolates were subjected for biofilm production assay and showed that all Salmonella isolates were able to form biofilm. Biofilm forming isolates were also subjected for antimicrobial resistance profiling. Three isolates were showed sensitivity against norfloxacin (92.5%). One was showing sensitivity against cefotaxime (90%). All the four isolates were showing complete resistant to ciprofloxacin, nalidixicacid, chloramphenical, gentamicin and cephalexin. Clindamycin and ofloxacin were showed sensitivity 20%. The finding of this study shows that coriander leaves are potential host for the transmission of Salmonella to human and animals. The ability of the isolates to form biofilm reveals the potential of the isolates to persist on the green leafy vegetables and the pathogenic status of the isolates as well as ability to resist antimicrobial chemotherapy.

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