Efficacy of purified antimicrobial peptides from lactic acid bacteriaagainst bovine mastitis pathogens

¹<p>Department of Dairy Science, College of Veterinary & Animal Sciences, Kerala Veterinary & Animal Sciences  University, Mannuthy-680 651, India.</p>

ABSTRACT

A study was carried out to assess the efficacy of purified antimicrobial peptides of lactic acid bacteria (LAB), isolated from partially decayed samples of food items, against selected clinical isolates of mastitis bacterial pathogens, viz., Escherichia coli (E.coli), Staphylococcus aureus (S.aureus) and Streptococcus agalactiae (S.agalactiae). The crude culture extracts (CCE) from LAB isolates were concentrated and purified by salting out method followed by dialysis. Partially purified peptides (Tp-5 CRp-2 CRp-3) from three LAB isolated from tomato and carrot showed a significantly higher inhibitory spectrum, with zone of inhibition ranging from 11.66±0.33 to 12.66±0.33 mm diameter against different test pathogens. The inhibitory efficiency of all the three peptides was found to be significantly superior to the peptides from reference strain Lactococcus lactis NCIM-2114. Minimum inhibitory concentration (MIC) of the purified peptide samples ranged between 0.79±0.09 to 1.11±0.09 µg/ml against different test pathogens. The activity of peptides was completely lost after treatment with protease, thereby confining their protein character.

KEYWORDS

REFERENCES

Aslam M., Shahid M., Rehman F. U., Naveed N. H, Batool A. I., Sharif S. and Asia A. (2011). Purification and characterization of bacteriocin isolated from Streptococcus thermophilus, African J. Microbiol. Res. 5: 2642-2648.

Barefoot, S. F. and Klaenhammer, T. R. (1983). Detection of activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl. Environ. Microbiol. 45:1808–1815.

Chassy, B. M. (1976). A gentle method for the lysis of oral Streptococci. Biochem. Biophys .Res. Commun. 68: 603-608.

Chen, Y., Ludescher, R.D. and Montville, T.J. (1997). Electrostatic interactions, but not the YGNGV consensus motif, govern the binding of pediocin PA-1 and its fragments to phospholipid vesicles. Appl. Environ. Microbiol. 63:4770-4777

Davey, G. P. and Richardson. B. C. (1981). Purification and some properties of diplococcin from Streptococcus cremoris 346. Appl. Environ. Microbiol. 41:84-89.

Harshada, J., Chaudhary B.L., Batish V.K. and Vij Shilpa. (2004). Inhibitory activity and characteristics of bacteriocin produced by Lactobacillus casei. Journal of Dairying Foods and Home Sciences. 23: 126-129.

Hastings, J. W., Sailer, M., Johnson, K., Ray, K. L., Vederas, J. C. and Stiles, M. E. (1991). Characterization of leuconocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidium. J. Bacteriol. 173:74-91

Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley J.T. and Williams, S.T. (1994). Bergey’s Manual of Determinative Bacteriology. (9thEd). Williams and Williams. Baltimore. 566p.

Kozak, W., Trzpil, M.R. and Dobrzanski, W. T. (1973). Preliminary observations on the influence of proflavin, ethidium bromide, and elevated temperature on the production of the antibiotic nisin by Streptococcus lactis strains. Bull. Acad. Pol. Sci. 21: 811-817.

Muriana, P.M. and Luchansky, J.B. (1993). Biochemical methods for purification of bacteriocins. In: Hoover, D.G and Steenson, L.R (eds.). Bacteriocins of Lactic Acid Bacteria. Academic Press, New York. pp.41-61.

Pingitore, E., Salvucci, V.E., Sesma, F. and Macías N.M. E. (2007). Different strategies for purification of antimicrobial peptides from Lactic Acid Bacteria (LAB). Trends Appl. Microbiol. 557-568.

Rahayu, E.S., Wardani, A.K. and Margino, S. (2004). Screening of lactic acid bacteria from meat and processed products as a producer of bacteriocin. Agritech. Yogyakarta.

Saidi, N., Hadadji M. and Guessas, B. (2011). Screening of bacteriocin producing lactic acid bacteria isolated from West Algerian goat’s milk. Global. J. Biotechnol. Biochem. 6: 154-161.

Sarkono, I., Faturrahman. And Sofyan, Y. (2010). Isolation and identification of lactic acid bacteria from abalone as a potential candidate of probiotic. Karnataka biosci. 2:38-42

Sharma,N. (2011). Purification of Bacteriocin by Bacillus subtilis R75, Food Technol. Biotechnol. 49: 169–176.

Tagg, J. R., Dajani, A. S. and Wannamaker, L. W. (1976). Bacteriological Reviews. 40 : 722.

Todorov, S.D. and Dicks, L.M.T. (2006). Screening for bacteriocin-producing lactic acid bacteria from boza, a traditional cereal beverage from Bulgaria Comparison of the bacteriocins, Process Biochem. 41: 11–19.

Vesterlund, S., Karp, M., Salminen, S. and Ouwehand, A.C. (2006). Staphylococcus aureus adheres to human intestinal mucus but can be displaced by certain lactic acid bacteria. Microbiology. 152: 1819–1826.

Wehr, H.M and Frank, J.F. (2004). Standard methods for the examination of dairy products (17 th Ed). American public health association, Washington DC. P.687.

Zheng. G. and Slavik, M.F. (1999). Isolation, partial purification and characterization of a bacteriocin produced by newly isolated Bacillus subtilis strain. Lett. Appl. Microbiol. 28:363-367

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Efficacy of purified antimicrobial peptides from lactic acid bacteria against bovine mastitis pathogens

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