<div style="text-align: justify;">The aim of this study is to determine the effect of different combinations of farm manure and farm manure on earthworm proliferation and earthworm manure production quantity and property. Accordingly, 100% horse manure (H), 100% cattle manure (C), 100% straw cattle manure &nbsp;(SC), 90% horse manure + 10% hen manure (H/H), 90% cattle + 10% hen manure (C/H) and 90% straw cattle manure + 10% hen manure (SC/H ) six research groups were designed. The highest level of worm growth in the experimental period was in group H, followed by SC group. The lowest values were found in C/H group and C group. In the amount of worm manure produced, the highest value was found in H/H group and the lowest value was found in SC group. As a result, it was found that 100% H and 100% SC groups made a better contribution to the increase in worm growth.&nbsp;</div>

<div style="text-align: justify;">Effect of Various Farm Manures and Straw Containing Cattle Manure on Worm and Worm Manure Production</div>

<div>The present study reports pathological conditions associated with Physaloptera sp. stomach worm infestation in Bengal monitor lizard (Varanus bengalensis). Four adult Bengal monitor lizards were rescued from the poachers and kept at Wild Animal Rescue and Rehabilitation Centre, Bannerghatta Biological Park, Bengaluru. Faecal samples from all were collected and examined. Physaloptera sp. ova were found in all faecal samples examined by sedimentation technique. One Bengal monitor lizard died during the period and systematically examined at necropsy for gross pathological lesions. Adult Physaloptera sp. nematodes were found to be firmly attached to the gastric mucosa causing erosive inflammatory foci. Histopathological examination of stomach revealed the presence of many immature and mature worms encapsulated within fibrous connective tissue. The intestinal villi were stunted and many cross sections of the worms could be demonstrated in the lumen. The worms also migrated to liver parenchyma and got encapsulated with fibrous connective tissue with infiltration of eosinophils in the sinusoids and the vascular tissue spaces. This seems to be the first report of Physaloptera sp. nematodiasis in Bengal monitor lizard kept under captivity for a period of 1.5 months.&nbsp;</div>

<div>Pathological Conditions Associated with Physaloptera Stomach Worm Infestation in Bengal Monitor Lizard (Varanus bengalensis)</div>

Background: Fall army worm is an invasive insect pest in India during year 2018 which caused severe damage to the crop and economic loss to the farmers. So, the main objective of the study is to evaluate the efficacy and effectiveness of seed treatment, biological and insecticidal applications for the management of fall army worm in maize.&nbsp; 
 
Methods: The study was conducted Kharif, 2018 and 2019 at Regional Agricultural Research Station, Chintapalle, Visakhapatnam during. It was evaluated with eleven treatments, after seed treatment with Cyantraniliprole 19.8% + Thiamethoxam 19.8 FS 5 ml per kg seed in 03 windows of application at 20, 35 and 50 DAS with battery operated sprayer. Data was collected on number of larva per plant (20 DAS), per cent plant infestation and % cob infestation, yield (q/ha) and cost benefit ratio. 
 
Result: Among three windows of applications, third window application with different insecticides was found most significant, followed by second window application (poison bait with Thiodicarb 75WP) in comparison to control (20.96). In 3rd window treatments application, spinetoram 11.7SC @ 0.5ml (T3 with 0.74 and T8 with 1.14 %) and spinosad 45SC @ 0.3 ml (T4 with 1.35 and T9 with 1.39 %), were found most significant, followed by&nbsp; &nbsp;Chlorantraniliprole 18.5SC (T2 with 1.76 and T7 with 1.25 %) in the management of fall army worm. Least Cob damage per cent (T3 with 7.23 and T8 with 8.00 %) and maximum yield (T3 with 72.55 and T8 with 73.00 q/ha) was recorded in Spinetoram 11.7 SC. Thus Spinetoram 11.7SC followed by Spinosad 45SC and Chlorantraniliprole 18.5SC found effective against S. frugiperda in Maize.

Management of the Fall Army Worm, Spodoptera&nbsp; frugiperda (J.E. Smith) in Maize

<div>Background: Gastrointestinal nematodosis is a major constraint in economic livestock production. Mostly synthetic anthelmintics are being used to overcome this problem. Besides synthetic anthelmintics, some plants have shown variable anthelmintic properties.&nbsp; 
 
Methods: Sixty goats infected with GI nematodes having 1500 EPG were selected and animals were divided into four groups T1 to T4 of 15 animals in each group. Group T1 was given two doses of fenbendazole @ 10 mg/kg BW per os at 15 days interval. In group T2, T3 and T4 herbal formulation &ldquo;Worm-X&rdquo; was given @ 0.5, 1.0 and 2.0 ml/kg BW orally respectively, at 15 days interval. Efficacy was assessed on the basis of percentage reduction in EPG count and the FECRT was conducted as per guidelines of WAAVP.&nbsp; 
 
Result: When FECR was compared 15 days post treatment, maximum reduction (77.25%) was observed in group T1 followed by group T2 (47.27%), T3 (70.19%), and T4 (72.01%). The reduction in FEC in groupT3 (70.19%)&nbsp;and T4 (72.01%) at 15 days post treatment and at 30 days post treatment were 87.75 and 89.32%, respectively were almost equal. Considering the cost effectiveness and WAAVP recommendations, it will be optimum to administer the drug at the rate of 1.0 ml/kg body weight in two dosages at 15-day interval. However, it is well known that now a day&rsquo;s modern anthelmintic drug efficacy has been reduced in many cases due to the development of anthelmintic resistance. Therefore, complementary or alternative solutions to the conventional chemical treatments have been implied offering novel approaches to the sustainable control of gastrointestinal nematodes in goats.</div>

<div>Efficacy of &ldquo;Worm-X&rdquo; Herbal Formulation against Naturally Infected Gastrointestinal Nematodes in Goats of Banaskantha District, Gujarat</div>

<div>Background: Ruminants have the distinct advantage of being able to eat forages and graze on land that is not suitable for growing crops. During breakdown of feed in rumen, 2 to 12 per cent of the gross energy consumed is converted to enteric CH4, which accounts for about 6 per cent of the world&rsquo;s anthropogenic greenhouse gas emissions. Anti nutritional factor (ANF) plays a vital role in rumen manipulation. It helps in mitigation of methane production and improves the feed efficiency.&nbsp; 
 
Methods: Twenty calves of 6-7 months old with an average body weight of 90.2&plusmn;4.0 kg were distributed randomly into four dietary treatment groups. The calves of the control group (T0) were fed on a basal diet as per ICAR (2013) feeding standard. The basal diet of T1, T2 and T3 were supplemented with NLP, MOP, CO@ 2 per cent on DM basis, respectively. Blood serum and fecal samples were collected and analyzed on 30th, 90th and 120th day of the experiment. The methane emission per animal was measured over fortnightly.&nbsp; 
 
Result: It was depicted that supplementation of ANFs as feed additives to the crossbred calves didn&rsquo;t not affect the blood serum indices but the worm load and methane emission in calves differ significantly (P&lt;0.05).</div>

Effect of Anti-nutritional Factors as Feed Additives on Enteric Methane, Blood Serum Indices and Worm Load in Crossbred Calves

Background: Ascaridia galli is pathogenic in chickens and causes substantially reduced growth rates, weight loss, low egg production and death in infected chickens. It is necessary to carry out research on the molecular identification of A. galli in chickens in traditional markets in Surabaya using the Polymerase Chain Reaction (PCR) method with the COX-1 mtDNA gene primer. 
&nbsp; 
Methods: This research was carried out from April to July 2023. The number of samples used in this research was 100 chicken small intestines taken from traditional markets in Surabaya, Indonesia. A. galli worms obtained from worm collections were identified according to the morphology of the A. galli worms. DNA extraction was carried out followed by PCR laboratory procedure. The specific primers utilized were designed to target the COX-1 gene and the amplified products were visualized in 1.5% agarose. 
&nbsp; 
Result: The A. galli worm has a long body posture. Male A. galli worms measure 30-50 mm long; while, adult female A. galli worms measure 65-80 mm long. The results of electrophoresis with a 2% agarose gel run for 30 minutes at 100 Volts and visualized under ultraviolet light using a UV Transilluminator (Geldoc) showed the presence of a single band of the COX-1 mtDNA gene in the 533 base pair. Infection with A. galli worms often causes reduced growth rates and decreased body weight, which is associated with increased worm weight.

Molecular Identification of Ascaridia galli in Chickens from Traditional Markets in Surabaya, Indonesia

Background: The safety of laboratory mice plays an important role in the success of laboratory experiments for correct and accurate results. Parasites are one of the most common diseases that affect most organisms. The worm Aspiculuris tetraptera is a common intestinal parasite of Mus musculus and rats, it is spread around the world. Aspiculuris tetraptera infection remains a problem for modern research groups; They should be excluded and monitored in rat populations due to their effects on animal health. 
 
Methods: The present study was conducted to assess the anthelmintic activity of Laurus nobilis extract in mice naturally infected with Aspiculuris tetraptera. Used was Thirty-six adult male and female (C57BL/6) mice, naturally infected with Aspiculuris tetraptera, were divided into six groups, each comprising six mice: Group 1, was the negative control (infected, untreated) and Groups 2, 3, 4 and 5 were treated with 50, 100, 200 and 400 &micro;g/ml of L. nobilis for 5 days respectively. Group 6 was treated with 10 mg/mL Albendazole for 3 days as a positive control. Different six treatments were used to test Aspiculuris tetraptera worms in vitro, Containing 4 concentrations (50, 100, 200 and 400 mg/ml) of L. nobilis extract. Ten actively moving adult worms were then placed in each petri dish at room temperature. A saline solution and 10 mg/ml of Albendazole were prepared and used as negative and positive controls. After treatment, observations were made by recording the death time for worms at 20, 40, 80, 120 and 180 minutes. Worms are considered dead if they do not move for 30 seconds after touching their body parts using a surgical needle and the petri dish is shaken. 
 
Result: The analysis of phytochemicals by FT-IR for alcoholic extracts of L. nobilis extracts revealed the presence of 15% effective chemical ingredients responsible for killing worms&rsquo; activity. In vitro, worms died in 20, 40, 80, 120 and 180 minutes. Attained 96% and 100% after 180 minutes at the highest concentrations (200 and 400 mg/ml). At the same time, the untreated group lasted for long hours. The effects of the plant extract (Laurus nobilis) on Aspiculuris tetraptera worms were studied in vivo at therapeutic doses of 50, 100, 200 and 400 mg/mL. and the concentration of 400 ml/kg showed the most lethal effects for worms, infected mice that did not receive drugs were compared with the highest concentration of the extract and the reference treatment. The attention showed significant differences P&le;0.05. The results showed that the mortality rate of worms taken from the intestines of the treated mice that were slaughtered three and six sdays after giving the treatment reached 96% and 100% in the concentration of 400 ml/kg of the extract and 89% and 97%for the mice that treated in 10 ml/kg of met Albendazole respectively. This research showed that herbal remedies could lead to new parasitic disease drugs, and their derivatives can be used for medication production and bioactivity improvement.

The Assessment of the Anthelmintic Activity of Laurus nobilis Extract in Mice Naturally Infected with Aspiculuris tetraptera

<div>Background: Internal parasitosis is the most important parasitism in small ruminants in tropical region. Synthetic anthelmintics are usually used for their control. Due to the emergence of resistance in worm population, the use of alternative methods such as plants bioactive molecules are developed. This study aimed at assessing anthelmintic activity of nine varieties of Cajanus cajan, a taniferous plant cultivated in West Africa.&nbsp; 
 
Methods: Leaves of the nine varieties of Cajanus cajan were dried, ground and hydroacetonic extracts were obtained by cold maceration at a concentration of 60 mg/ml. Live adult of Haemonchus contortus were obtained from sheep&rsquo;s stomach and exposed, in triplicate, to the following solutions: hydroacetonic extracts (60 mg/ml), hydroacetonic extracts (60 mg/ml) associated with polyvinylpyrrolidone (PVP) (50 mg/ml), Levamisole (20 mg/ml) and phosphate buffered saline (PBS) solution. Worm death time was recorded for each treatment.&nbsp; 
 
Result: Worms exposed to Levamisole recorded the shortest death time about 9.73&plusmn;1.77 min. The potent of the extracts induced worm death time of 64.83&plusmn;4.73 min while the least efficient induced a death time of 156.50&plusmn;23.20 min. Worms in the PBS solution were still alive after 24 hours of exposure. These results indicated that the four varieties of C. cajan used in this study have promising wormicidal. Besides that, the effect of tannins were not the only compound responsible for the anthelmintic activity.</div>

Anthelmintic Activity of Nine Varieties of Cajanus cajan (L.) Millsp on Haemonchus contortus from Sheep

<div>Background: Helminth parasites of ruminants are a major factor limiting animal production in many parts of the world. Dicrocoelium dendriticum, causes liver fluke disease in ruminants and is of zoonotic and economic importance. D. dendriticum lives in the adult stage, in the bile ducts and gallbladders of cattle, sheep, goats and pigs. Dicrocoeliasis causes severe pathological changes in the liver and bile system such as abscesses, granulomas and fibrosis. The objective of this study was to identify the anthelmintic activity of Vitis vinifera leaf extract against D. dendriticum and comparison with some drugs used against internal parasites. 
 
Methods: A study was performed to evaluate the anthelmintic activity of the methanolic extract of V. vinifera leaf against D. dendriticum using an adult worm motility test. Six graduated concentrations of extract (25, 50, 75, 100, a125 and 150 mg/ml), were tested at different periods and changes over time in the viability of worms were registered for 2, 4 and 6 hr. Normal RPMI-1640 medium and Albendazole were used as negative and positive control, respectively. 
 
Result: A significant elevation in mean inhibition of adult worm motility beginning 2 h post-exposure with 150 mg/ml of V. vinifera leaf extract and Albendazole. After 4 h of the exposure time, Albendazole and concentrations of 125 and 150 mg/ml of the extract resulted in significantly higher inhibited motility compared to the remaining concentrations below 125 mg/ml. The lower concentration (25 mg/ml) was significantly more lethal than the negative control (RPMI-1640 medium) at 6 h of exposure. Within 6 h after exposure, concentrations of 100, 125 and 150 mg/ml of V. vinifera leaf extract were more effective against adult worms than the remaining concentrations below 100 mg/ml. As the concentration and duration of exposure increased, the mortality of adult worms and also increased death for all parasites (P&lt;0.05). Each concentration damages the tegument and muscles of D. dendriticum. The increase in concentration is proportional to the increase in damage to the integument. The results of this investigation demonstrated the anthelmintic action of V. vinifera leaf extract.</div>

In vitro Anthelmintic Activity of Vitis vinifera Leaf Extract on Dicrocoelium dendriticum

<div>Background: A two-year study was conducted at Giza Agricultural Research Station, Agricultural Research Center (ARC), Egypt, to evaluate the productivity of six genotypes of soybeans under three mineral nitrogen (N) fertilizer rates, as well as their resistance to insects, in comparison to four check varieties. 
 
Methods: The study took place during the 2021 and 2022 summer seasons. The treatments included three mineral N fertilizer rates (N1=67% of N fertilizer rate &ldquo;71.4 kg N/ha&rdquo;, N2=33% of N fertilizer rate &ldquo;35.7 kg N/ha&rdquo; with seed inoculation by Bradyrhizobium japonicum and N3=100% of N fertilizer rate of the recommended rate &ldquo;107.1 kg N/ha&rdquo;) and ten soybean genotypes (H4L4, H6L198, H18L54, H29L115, H129, Misr 10, along with Dr101 and Giza 111 &ldquo;resistant&rdquo; and Giza 82 and Crawford &ldquo;susceptible&rdquo;). The experiment used a split plot design with three replications. The main plots were assigned the mineral N fertilizer rates, while the subplots, were assigned the soybean genotypes.&nbsp; &nbsp; 
 
Result: Fewer cotton leaf worms, whiteflies and leaf miners were found after applying N1 or N2. In the 6th week from sowing, Misr 10 and Dr 101 had fewer cotton leaf worms and whiteflies. In contrast, throughout the 7th, 8th and 9th weeks from sowing, Misr 10 and H6L198 had fewer cotton leaf worms. Misr 10 had fewer leaf miners in every week and fewer whiteflies in the 7th, 8th and 9th weeks from sowing. In the 6th week of the first season, Misr 10, Dr101, H18L54 and Giza 111 that received N1 or N2 had fewer cotton leaf worms; in the 7th and 9th weeks from sowing, Misr 10, Dr101 and H6L198 harbored fewer whiteflies. The lowest pod weight/plant, seed yield/plant, 100-seed weight, seed yield/ha and HI were obtained by using N3. Higher biological yield/ha, seed yield/plant, 100-seed weight, seed yield/ha and HI were attained by Misr 10. In the second season, seed yield per plant and seed yield per hectare were significantly affected by the interaction between soybean genotypes and mineral N fertilizer rates. Growing Misr 10 with 67 per cent N of the recommended rate increased seed yield/plant and seed yield/ha, along with fewer cotton leaf worm, whiteflies and leaf miners compared to the commercial cultivar Giza 111 receiving the recommended mineral N fertilizer rate.</div>

Effect of Various Farm Manures and Straw Containing Cattle Manure on Worm and Worm Manure Production

Pathological Conditions Associated with Physaloptera Stomach Worm Infestation in Bengal Monitor Lizard (Varanus bengalensis)

Management of the Fall Army Worm, Spodoptera frugiperda (J.E. Smith) in Maize

Efficacy of “Worm-X” Herbal Formulation against Naturally Infected Gastrointestinal Nematodes in Goats of Banaskantha District, Gujarat

Effect of Anti-nutritional Factors as Feed Additives on Enteric Methane, Blood Serum Indices and Worm Load in Crossbred Calves

Molecular Identification of Ascaridia galli in Chickens from Traditional Markets in Surabaya, Indonesia

The Assessment of the Anthelmintic Activity of Laurus nobilis Extract in Mice Naturally Infected with Aspiculuris tetraptera

Anthelmintic Activity of Nine Varieties of Cajanus cajan (L.) Millsp on Haemonchus contortus from Sheep

In vitro Anthelmintic Activity of Vitis vinifera Leaf Extract on Dicrocoelium dendriticum

Response of Some Soybean Genotypes to Insect Infestation under Three Mineral Nitrogen Fertilizer Rates