Efficacy of “Worm-X” Herbal Formulation against Naturally Infected Gastrointestinal Nematodes in Goats of Banaskantha District, Gujarat

DOI: 10.18805/IJAR.B-4116    | Article Id: B-4116 | Page : 839-843
Citation :- Efficacy of “Worm-X” Herbal Formulation against Naturally Infected Gastrointestinal Nematodes in Goats of Banaskantha District, Gujarat.Indian Journal of Animal Research.2021.(55):839-843
H.R. Parsani, B.S. Chandel, K.P. Shyma  husen46@gmail.com
Address : Department of Parasitology, College of Veterinary Science and Animal Husbandry. S.D. Agricultural University, Sardarkrushinagar-385 506, Gujarat, India.
Submitted Date : 4-03-2020
Accepted Date : 6-06-2020


Background: Gastrointestinal nematodosis is a major constraint in economic livestock production. Mostly synthetic anthelmintics are being used to overcome this problem. Besides synthetic anthelmintics, some plants have shown variable anthelmintic properties. 
Methods: Sixty goats infected with GI nematodes having 1500 EPG were selected and animals were divided into four groups T1 to T4 of 15 animals in each group. Group T1 was given two doses of fenbendazole @ 10 mg/kg BW per os at 15 days interval. In group T2, T3 and T4 herbal formulation “Worm-X” was given @ 0.5, 1.0 and 2.0 ml/kg BW orally respectively, at 15 days interval. Efficacy was assessed on the basis of percentage reduction in EPG count and the FECRT was conducted as per guidelines of WAAVP. 
Result: When FECR was compared 15 days post treatment, maximum reduction (77.25%) was observed in group T1 followed by group T2 (47.27%), T3 (70.19%), and T4 (72.01%). The reduction in FEC in groupT3 (70.19%) and T4 (72.01%) at 15 days post treatment and at 30 days post treatment were 87.75 and 89.32%, respectively were almost equal. Considering the cost effectiveness and WAAVP recommendations, it will be optimum to administer the drug at the rate of 1.0 ml/kg body weight in two dosages at 15-day interval. However, it is well known that now a day’s modern anthelmintic drug efficacy has been reduced in many cases due to the development of anthelmintic resistance. Therefore, complementary or alternative solutions to the conventional chemical treatments have been implied offering novel approaches to the sustainable control of gastrointestinal nematodes in goats.


FECRT Gastrointestinal nematodes Goat Herbal formulation Worm-X


  1. Arora, N., Vihan, V.S., Sharma, S.D. and Kumar, A. (2007). Evaluation of anthelmintic activity of Azadirachta indica (Neem) against Haemonchus contortus infection in goats. Indian J. Vet. Med. 27: 95-98.
  2. Asaka, Y., Kubota, T. and Kulkarni, A.B. (1977). Studies on a bitter principle from Vernonia anthelmintica. Phytochem. 16: 18-38.
  3. Chothani, D.L. and Vaghasiya, H.U. (2011). A review on Balanites aegyptiaca Del (desert date): phytochemical constituents, traditional uses and pharmacological activity. Pharmacogn Rev. 5(9): 55-62.
  4. Cole, G.C., Bauer, C., Borgsteede, F.H.M., Geertsm, S., Klei, T.R., Taylor, M.A. and Waller, P.J.(1992). World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance, Vet. Parasitol. 44(1-2): 65-44.
  5. Duncan, D.B. (1955). Multiple range and multiple F tests. Biometrics. 11:1-42.
  6. Duvey, B.K. (2013). Evaluation of phytochemical constituents and anthelmintic activity of aerial part of Trichosanthes tricaspidata Lour. Int. J. Pharm. Phytopharmacol. Res. 3(2): 104-106.
  7. Gelot, I.S., Singh, V., Shyma, K.P. and Parsani. H.R. (2016). Emergence of multiple resistances against gastrointestinal nematodes of Mehsana-cross goats in a semi-organized farm of semi-arid region of India. J. Appl. Anim. Res. 44 (1): 146-149.
  8. Githigia, S.M., Thamsberg, S.M., Maing, N. and Munyua, W.K. (2005). The epidemiology of gastrointestinal nematodes in goats in the low potential areas of Thinka district, Kenya. Bull. Anim. Hlth. Prod. Africa. 53: 5-12.
  9. Githiori, J.B., Hogland, J., Waller, P.J. and Baker, R.L. (2004). Evaluation of anthelminticactivity of some plants used as livestock dewormers against Haemonchus contortus infection in sheep. Parasitology. 129: 245-253.
  10. Grade,J.T., Tabuti, J.R.S. and Van Damme P (2009). Four footed pharmacists: Indications of self-medicating livestock in karamoja, Uganda. Econ Bot. 63: 29-42.
  11. Gupta, M.K., Rao, M.L.V., Dixit, P., Shukla, P.C., Baghel, R.P.S. and Dixit, A.K. (2016). Economic impact of anthelmintic therapy in goats naturally infected with gastrointestinal nematodes. Environ. Ecol. 34 (4D): 2498-2500.
  12. Hordegen, P., Cabaret, J., Hertzberg, H., Langhans, W. and Maurer, V. (2006). In vitro screening of six anthelmintic plant products against larval Haemonchus contortus with a modified methyl-thiazolyl-tetrazolium reduction assay. J Ethnopharmacol. 108: 85-89.
  13. Hutchens, T. and Chappell, M. (2004). Gastro-intestinal parasite survival kit for goats. Retrieved Feb 2, 2007, from online http://www.uky.edu/Ag/AnimalSciencesgoats/presentations/ parasitekit0104.pdf.
  14. Iqbal, Z., Lateef, M., Jabbar, A., Ghayur, M.N. and Gilani, A.H. (2006). In vitro and in vivo anthelmintic activity of Vernonia anthelmintica seeds against trichostrongylid nematodes of sheep. Phytother Res. 44: 563-567.
  15. Iqbal, Z., Sarwar, M., Jabbar, A., Ahmed, S., Nisa, M., Shajid, M.S., Khan, M.N., Mufti, K.A. and Yaseen, M. (2007). Direct and indirect effect of condensed tannins in sheep. Vet. Parasitol. 144: 125-131.
  16. John, J., Mehta, A., Shukla, S. and Mehta, P. (2009). A report on anthelmintic activity of Cassia tora leaves. Songklanakarin J. Sci. Technol. 31(3): 269-271 
  17. Kumar, D., Mishra, S.K. and Tripathi, H.C. (1995). Possible mechanism of anthelmintic action of Palasonin on Ascardiagalli. Indian J Pharmacol. 27: 161-166.
  18. Lambertini, B., Piva, R., Khan, M.T.H., Lampronti, I., Bianti, N., Borgatti, M. and Gambari, R. (2004). Effects of extracts from Bangladeshi medicinal plants on in vitro proliferation of human breast cancer cell lines and expression of estrogen receptor alpha gene. Int J Oncol. 24: 419.
  19. Moudgil, A.D. and Singla, L.D. (2018). Molecular confirmation and anthelmintic efficacy assessment against natural trichurid infections in zoo housed non-human primates. J. Med. Primatol. 47 (6): 388-392.
  20. Murali, J., Maheswari, R., Syed Muzammil, M. and Asogan, G. (2014). Anthelmintic activity of leaves extract of Andrographis paniculata Nees. Int. J. Pharmacogn 1(6): 404-408 DOI: 10.13040/IJPSR.0975-8232. IJP.
  21. Nkunya, M.H., Weenen, H. and Bray, D.H. (1990). Chemical evaluation of Tanzanian medicinal plants for the active constituents as a basis for the medicinal usefulness of    the plants. In: Proceedings of International Conference on Traditional Medicinal Plants. Mshigeni, K.E., Nkuanya, M.H., Fupi, V., Mahunnah, R.L. and Mshiu, E.N, editors. Arusha: 1. pp. 101-11.
  22. Papadopoulos, E. (2008). Anthelmintic resistance in sheep nematodes. Small Ruminant Res. 76: 99-103. DOI: 10.1016/j.small rumres.2007.12.012.
  23. Pawar, P.D., Singla, L.D., Kaur, P. Bal, M.S. and Javed, M. (2019). Evaluation and correlation of multiple anthelmintic resistance to gastrointestinal nematodes using different faecal egg count reduction methods in small ruminants of Punjab, India. Acta Parasitol. 64: 456-463.
  24. Prashant, D., Asha, M.K., Amit, A. and Padmaja, R. (2001). Short report: anthelmintic activity of Butea monosperma. Fitoterapia. 72: 421-422. doi: 10.1016/S0367-326X (00) 00333-6. 
  25. Prashith Kekuda, T.R., Praveen Kumar, S.V., Nishanth, B.C. and Sandeep, M. (2009). In vitro athelmintic activity of aqueous extract of Embelia ribes. Bio Technology An Indian Journal. 3(2): 87-89.
  26. Singh, D., Swarnkar, C.P. and Khan, F.A. (2002). Anthelmintic resistance in gastrointestinal nematodes of livestock in India. J. Vet Parasitol. 16: 115-130.
  27. Singh, E., Kaur, P., Singla, L.D., Sankar, M. and Bal, M.S. (2019). Molecular detection of benzimidazole resistance in Haemonchus contortus of sheep in Punjab, India. Indian J. Anim. Sci. 89(12): 1322-1326.
  28. Singh, G., Singh, R.,Verma,P.K., Singh, R. and Anand, A. (2015). Anthelmintic efficacy of aqueous extract of Butea monosperma (Lam.) Kuntze against Haemonchus contortus of sheep and goats. J. Parasit Dis. 39(2): 200-205. 
  29. Singh, R., Bal, M.S., Singla, L.D. and Kaur, P. (2017). Detection of anthelmintic resistance in sheep and goat against fenbendazole by faecal egg count reduction test. J. Parasit. Dis. 41(2): 463-466.
  30. Singh,S., Ansari, N.A., Srivastava, M.C., Sharma, M.K. and, Singh, S.N. (1985). Anthelmintic activity of Vernonia anthelmintica. Indian Drugs. 22: 508-511.
  31. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods, 7th edn., Oxford and IBH Publishing Co., Calcutta, p 445
  32. Soulsby, E.J.L. (1986). Helminth, Arthropods and Protozoa of domesticated animals (Text book) Williams and Wilkins Company, Baltimore 7th Edn., Tindal and Cassel LTD. 7-8 Henrietta Street London WO2 pp 212-242.
  33. Sunandhadevi, S., Rao, M.L.V., Dixit, P., Dixit, A.K., Shukla, P.C. and Baghel, R.P.S. (2017). In vivo anthelmintic activity of a herbal formulation against naturally acquired gastrointestinal nematodes in goats. Environ. Ecol. 35 (2A): 933-935.
  34. Sykes, A. (1994). Parasitism and production in farm animals. Anim. Prod., 59: 155-172. DOI: 10.1017/S0003356100007649.
  35. Torres- Acosta J.F.J and Hoste H. (2008). Alternative or improved methods to limit gastro-intestinal parasitism in grazing sheep and goats. Small Ruminant Research. 77(2-3): 159-173. 
  36. Verma, A.K., Sash, V.R.B. and Agrawal, D.K. (1995). Feeding of water washed neem (Azadirachta indica) seed kernel cake to growing goats. Small Rumin. Res. 15: 105-111.
  37. Waghmare, S.P., Kadam, Y., Kolte, A.Y., Mode, S.G., Mahalle, G.P. and Mude, S. (2009). Anthelmintic efficacy of herbal formulation against hemonchosis in sheep. Indian J. Vet Med. 29: 29-31.
  38. Zajac, A.M. and Conboy, G.A. (Eds.). (2012). Veterinary Clinical Parasitology. John Wiley and Sons.

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