<div>Bovine Respiratory diseases are responsible for economic losses in livestock industry worldwide. The present study aiming to investigate the occurrence of respiratory viral antigens of Bovine herpesvirus-1 (BoHV-1), Bovine respiratory syncytial virus (BRSV), Bovine viral diarrhoea virus (BVDV) and Bovine parainfluenza 3 virus (BPI3V) in bovines and describe the distribution of respiratory viruses in unvaccinated animals in Punjab. A total of 55 nasal swab samples were collected from the cattle and buffaloes exhibiting the clinical signs of respiratory disease. All samples were tested for the detection of all four viral antigens by commercially available sandwich ELISA kit. BoHV-1 and BPI3V antigen were detected in 1 and 3 samples with the percentage of 1.8 and 5.4, respectively, whereas none of the sample was found positive for BRSV and BVDV antigen. The present study concluded the existence of BoHV-1 and BPI3V infection in respiratory tract and plays the important role in the respiratory viral infections of bovines. This study suggests the prevention measures should be taken to control the economic losses due to respiratory diseases in cattle and buffaloes.</div>

Detection of Respiratory Viral Antigens in Nasal Swabs of Bovine by Sandwich ELISA

<div>Respiratory affections are common in dogs. Very young and older dogs are at greater risk as compared to the adult ones. Constant exposure of the respiratory tract to the infectious agents, reaching the upper and lower respiratory tract aerogenously or hematogenously, makes the respiratory system vulnerable to injury. Clinical signs mainly seen in upper respiratory tract diseases involves nasal discharge (serous, mucopurulent or hemorrhagic), sneezing, facial deformity, stertor, lethargy, inappetence and rarely, central nervous system signs whereas additional signs present in lower tract diseases range from dyspnoea, costal or abdominal respiration, cough, nasal discharge and congestion, edema, consolidation of lungs and weight loss. Advanced tests involved in diagnosis of respiratory problems in veterinary practicpe are rhinoscopy, bronchoscopy, transthoracic fine needle aspiration cytology (FNAC) transtracheal wash and bronchoalveolar lavage fluid cytology and culture. This review discusses the causes of upper and lower respiratory tract disorders of canines and various diagnostic tests involved in their diagnosis.</div>

Respiratory Diseases and Their Diagnosis in Dogs: A Review

<div>Background: Bovine herpes virus type 1 (BoHV-1) the causative agent of Infectious bovine rhinotracheitis (IBR) is of great concern to dairy farmers and veterinarians due to great economic impact caused by the virus in terms of loss of production and abortion. Therefore, the study was planned to detect the virus circulating in the bovine population of the region under study. This virus is an important pathogen of bovine respiratory diseases. The aim of the present study was to isolate the BoHV-1 virus from the upper respiratory tract of bovines. 
 
Methods: A total of 13 nasal swab samples were subjected to virus isolation in Madin Darby Bovine Kidney (MDBK) cells lines. A PCR assay was applied to confirm the BoHV-1 DNA by targeting gI glycoprotein gene in isolates. 
 
Result: Total two IBR virus isolates were recovered from 13 nasal swab samples of bovines. Both isolates exhibited cytopathic effects i.e. clumping and rounding of cells. A 468 base pair of amplified product from both isolates confirmed the IBR virus in gI gene specific PCR for BoHV-1. This study concludes that IBR virus exists among cattle population of Punjab and it is present in the upper respiratory tract of infected animal and shed through respiratory route. The PCR detection assay for detection of BoHV-1 from nasal swab samples is considerably more sensitive than virus isolation.</div>

Isolation and Molecular Characterization of Bovine Herpes Virus Type 1 from Upper Respiratory Tract of Bovines

<div style="text-align: justify;">Aetio-pathological studies of digestive and respiratory affections was undertaken in cattle calves received for post mortem examination during a period of seven months (September, 2015 to March, 2016). Maximum age-wise mortality was noticed in age group of 1 to 3 months. Sex-wise mortality in cattle calves was more in females as compared to males. System-wise causes of death/mortality were highest due to combined involvement of both the digestive and respiratory systems. Pneumo-enteritis was the main affection noticed when there was combined involvement of both digestive and respiratory systems. Bacteriological studies of different samples collected from carcasses of cattle calves revealed that maximum bacterial species isolated was E. coli followed by Proteus spp., Klebsiella spp. and Staphyloccocus spp. Maximum number of bacteria species were isolated from intestine followed by lungs, heart blood and tracheal swab. The results of in-vitro drug sensitivity revealed that most of bacterial strains were sensitive to gentamycin and resistant to tetracycline. Examination of faecal samples of diarrhoeic/diseased and dead cattle calves revealed that Ascaris ova and Eimeria spp. was the major infection.</div>

Aetio-pathological studies of digestive and respiratory affections in cattle calves

Background:&nbsp;Mannheimia is a Gram-negative, facultative anaerobe, non-spore forming and non-motile bacteria belongs to the family Pasteurellaceae. Caprine bacterial pneumonia is most often caused by Mannheimia haemolytica (previously known as Pasteurella haemolytica) and Pasteurella multocida which are more frequently associated with the outbreak of acute pneumonia and death of goats, particularly young once. Mannhemia is an opportunistic pathogen that particularly inhabits the nasopharynx and tonsils of goat and sheep. The present study was aimed to study Mannheimiosis in Guwahati, Assam in order to diagnose and control the disease among goats. 
 
Methods: The present investigation was conducted during the year 2021-2022 in Guwahati, Assam. A total of 51 pneumonic lung samples were collected from nearby slaughter houses and during necropsy at Department of Pathology, C.V.Sc., A.A.U., Khanapara, Guwahati-22. All the samples were subjected to primary isolation in blood agar and MacConkey&#39;s agar and further confirmation was made using PCR. 
 
Result: Out of total 51 pneumonic lung samples examined seven (13.72%) cases were found positive for Mannheimia haemolytica infection where 5 cases were found to be associated with other respiratory pathogens.&nbsp;Visible gross lesions observed during necropsy were broncho-interstitial pneumonia, suppurative pneumonia and fibrinous pneumonia with hemorrhagic tracheitis. Histopathological examination revealed presence of characteristic oat cells, intravascular thrombosis, fibrin balls, oedema, necrosis and polymorphonuclear cell infiltration in the lungs. Bacteriological culture for isolation and identification of Mannheimia haemolytica showed &beta; haemolytic zone on MLA plates. Confirmatory diagnosis was made using PCR targeting 16s rRNA gene with amplicon size 1500 bp and Lkt gene with amplicon size 206 bp. The phylogenetic analysis of 16s rRNA gene from positive sample showed percent identity of above 97% with other strains of Mannheimia haemolytica present in NCBI website. The variability in the lesions observed in the present study it can be concluded that a mixed infection with reduced resistance of the animals contributed to the development of pneumonia rather than Mannheimia haemolytica alone and PCR can be used as reliable technique for confirmatory diagnosis of respiratory mannheimiosis.

Pathomorphological and Molecular Studies of Respiratory Mannheimiosis in Goats

<div>Background- Evaluation of transtracheal wash (TTW) has been proved beneficial in the diagnosis and understanding the pathology of lower respiratory tract infection in species like equines and dogs. However, its diagnostic potential in buffaloes has not been explored much. This study elucidated the cytological and microbiological features of TTW in buffaloes. 
 
Methods- TTW was collected from 36 buffaloes (26 diseased and 10 healthy control) after adequately restraining and using baby feeding tube after giving a small stab incision at ventral neck region. After collection, TTW were subjected to cytological studies following staining with Leishman stain. TTW was also sent aseptically to laboratory for bacteriological culture. 
 
Results- The lower respiratory tract affections like aspiration pneumonia, suppurative pneumonia, fibrinopurulent pneumonia, chronic pneumonia and tuberculous pneumonia were diagnosed based on cytology of TTW in correlation with history and comprehensive clinical examination. The mean cell number (cells/HPF) in TTW of diseased buffaloes were significantly higher than control groups. In control groups, the predominant cells were alveolar macrophages followed by neutrophils, epithelial cells, lymphocytes and other cells which includes mast cells, fibroblasts and unidentified nucleated cells. Neutrophils were the predominant cells followed by macrophages in TTW of buffaloes diagnosed with suppurative, fibrinopurulent and aspiration pneumonia, whereas, alveolar macrophage were predominant in TTW of chronic pneumonia and tuberculous pneumonia affected animals. Four out of ten TTW samples from healthy animals were evidenced bacterial growth in which Staphylococcus spp. was the predominantly isolated bacteria. TTW samples from 20 out of 26 diseased buffaloes were found positive for bacterial culture from which a total of 30 bacterial isolates were obtained which include Staphylococcus aureus, Klebsiella pneumonia, Pasteurella multocida, Streptococcus spp., E. coli and Bacillus spp. Thus, TTW provides vital information about the ongoing pathogenesis in respiratory tract infection. It is suggested that, cytological and bacteriological culture of TTW samples should be carried out during the diagnosis and therapeutic intervention of respiratory tract infection in buffaloes.</div>

Laboratory Evaluation of Transtracheal Wash (TTW) in Buffaloes Affected with Lower Respiratory Tract Infections

<div>Background: Porcine reproductive and respiratory syndrome (PRRS) is considered as an economically important viral disease of pigs worldwide. India has reported the first outbreak of PRRS in the pig population of Mizoram state to the Office International des Epizooties (OIE) on the 26 June, 2013. Although the disease appeared to be contained in the Mizoram state, several severe outbreaks of the disease has been reported in subsequent years.&nbsp; The present study has determined the current status of porcine reproductive and respiratory syndrome in the pig population of Mizoram state and its diagnosis by molecular and immunohistochemistry technique. 
 
Methods: A total number of 365 pigs of different age groups from 5 organized and 35 backyard farms located in different districts of Mizoram were studied. A detailed necropsy was conducted on 88 dead pigs. Gross lesions were recorded and representative tissue samples were collected for histopatholgical, immunohistochemical studies and preserved at -80&deg;C for molecular diagnosis. 
 
Result: Respiratory signs characterized by difficulty in breathing accompanied with high fever were the clinical signs observed in affected pigs. Severe haemorrhages in lungs with interstitial pneumonia, haemorrhagic lymphadenitis with lymphoid depletion in lymphoid organs were the most commonly observed lesions followed by haemorrhagic myocarditis and nephritis. Reverse transcription PCR targeting 300 bp fragments of ORF7 gene and real-time PCR targeting 152 bp fragments of ORF7 gene of PRRSV from the tissue extracted RNA (lung, lymph node, spleen) confirmed the outbreaks of PRRSV in different backyard and organized swine herds. The PRRSV antigen was demonstrated by immunohistochemistry in alveolar macrophages in lungs and infiltrating macrophages in spleen and lymph nodes tissues respectively.&nbsp;</div>

Molecular Diagnosis of Porcine Reproductive and Respiratory Syndrome Outbreaks in Pigs of Mizoram State

<div>Background: Pathomicrobial and molecular investigations of respiratory system diseases was undertaken on twelve adult buffalo carcasses received for necropsy examination at post mortem facility of Department of Veterinary Pathology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana during the period from August, 2018 to February, 2019.&nbsp; 
 
Methods: After collection of the samples, laboratory work was undertaken in the laboratories of Department of Veterinary Pathology, Department of Animal Biotechnology and Central Laboratory of the College, LUVAS, Hisar, Haryana (India) in the year 2018-2019 regarding the examination of the clinical history, pathological, microbiological and molecular investigations. 
 
Result: The gross pathological changes observed in lungs were variable degree of vascular changes, mild to severe consolidation, fibrin deposition along with adhesions to the thoracic wall. Histopathological findings revealed abnormalities of inflation such as pulmonary emphysema, atelectasis, pulmonary congestion and haemorrhages which was associated with different types of pneumonia viz. fibrinous bronchopneumonia, suppurative giant cell pneumonia, interstitial pneumonia and serous pneumonia. Lung, heart blood and tracheal swab samples collected from the buffalo carcasses revealed eighteen bacterial isolates which were identified by Vitek 2 system. These include E. coli (11 isolates), Salmonella enteric enterica (2 isolates), Acinetobacter ursingii (1 isolate), Staphylococcus haemolyticus (1 isolate), Staphylococcus sciuri (1 isolate), Staphylococcus warneri (1 isolate) and Staphylococcus hominis (1 isolate) mostly belonging to opportunistic pathogen category. E. coli serotypes confirmed from these cases were O83, O149 and O8. The results of in vitro drug sensitivity testing revealed that most of bacterial strains were found sensitive to cefoperazone/sulbactum and co-trimoxazole. Molecular studies confirmed Bovine Herpes Virus-1 (BHV-1) infection in one case with Fibrinous bronchopneumonia through real-time quantitative PCR indicating the prevalence of the infection in the state.</div>

Pathomicrobial and Molecular Investigations of Respiratory System Diseases Affecting Buffaloes

<div>Mycoplasma infection of the respiratory tract of goats is prevalent worldwide including the South Asian sub-continent. Owing to intensive and large scale goat farming, the incidence of the disease is on an increase. Among various species of mycoplasma, Mycoplasma capricolum subspecies Capri pneumoniae is increasingly incriminated in Contagious Caprine Pleurapneumonia (CCPP) in goat populations with considerable economic fallout in the form of high morbidity and mortality. The disease manifestations in caprines are recorded as anorexia, high febrile reaction and respiratory embarrassment in the shape of clinical dyspnoea, polypnea, paroxysmal cough and sero-purulent nasal discharges. The disease is thus contracted by the healthy animals through aerosol, contaminated feed and water sources in the herd premises, without a protective immunity and that the conferred immunity in recovered cases being short-lived. The true lesions of CCPP are confined to the lung alveolar tissues of infected goats, which distinguish it from other respiratory diseases of small ruminants caused by the members of the Mycoplasma mycoides cluster.&nbsp;Atypical pneumonia caused by the mycoplasma infection of goats, also known as Contagious Caprine Pleuropneumonia (CCPP) has been more often reported from Africa and Asia than Europe. Classical, acute CCPP attributed to Mycoplasma capricolum subsp. Capri pneumoniae, originally known asthe F38 biotype (World Organisation for Animal Health, 2008) causes heavy kid mortality. Two other organisms in this group, M. mycoides subsp.&nbsp;capri and&nbsp;M. mycoides subsp.&nbsp;mycoides large-colony type, can cause disease in small ruminants that clinico-pathologically mimics CCPP&nbsp;but may have extra pulmonary signs and lesions, sometimes. Mycoplasma Capri pneumoniae and other members of the M. mycoides cluster cross-react in serological tests and share biochemical and genetic similarities. The most favourable epidemiological scenario in the Sub-continent is the hot humid climate during monsoons. The diversity and multi-etiological subspecies involved in the disease is detrimental in the development of an effective vaccine even though in some places a liquid vaccine is presently in use. At other places, anti-mycoplasmal antibiotics of aminoglycoside and fluoroquinolone and perhaps the macrolide groups remain to be the main option in preventing flock mortalities.</div>

​Caprine Respiratory Mycoplasmosis (Contagious Caprine Pleuropneumonia CCPP)- A Global Perspective of the Disease, Epidemiology, Diagnosis, Chemotherapy and Immunization: A Review

<div>To evaluate respiratory metabolism, energy utilization and enzymatic responses in common Chinese cuttlefish (Sepiella maindroni) to hypoxia, juvenile Chinese cuttlefish (26.66 &plusmn; 2.52 g) were exposed to a 1.4-L airtight respiration chamber until suffocation for 83.4 min. The results showed that dissolved oxygen (DO) was mainly consumed in the closed chamber and the rates of oxygen consumption and ammonia excretion decreased significantly with decreasing DO content. The suffocation point for the cuttlefish was 1.16 &plusmn; 0.10 mg&middot;L-1. The O:N ratio increased significantly with decreasing DO content. The TP content in the hemolymph at 83.4 min was significantly lower than that at other time points; however, the highest GLU content was noted at 83.4 min, the highest AST and ALP activities occurred at 60 min and 83.4 min, respectively. These results indicated that although hypoxia inhibits respiratory metabolism, the cuttlefish demonstrated a capacity for energy mobilization under hypoxic conditions.</div>

<div>Respiratory Metabolism, Energy Utilization and Biochemical Responses of Juvenile Common Cuttlefish (Sepiella maindroni) to Hypoxia</div>