Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 54 issue 3 (march 2020) : 354-358

Detection of Respiratory Viral Antigens in Nasal Swabs of Bovine by Sandwich ELISA

Rakhi Gangil, Gurpreet Kaur, P.N. Dwivedi
Cite article:- Gangil Rakhi, Kaur Gurpreet, Dwivedi P.N. (2019). Detection of Respiratory Viral Antigens in Nasal Swabs of Bovine by Sandwich ELISA. Indian Journal of Animal Research. 54(3): 354-358. doi: 10.10.18805/ijar.B-3769.
Bovine Respiratory diseases are responsible for economic losses in livestock industry worldwide. The present study aiming to investigate the occurrence of respiratory viral antigens of Bovine herpesvirus-1 (BoHV-1), Bovine respiratory syncytial virus (BRSV), Bovine viral diarrhoea virus (BVDV) and Bovine parainfluenza 3 virus (BPI3V) in bovines and describe the distribution of respiratory viruses in unvaccinated animals in Punjab. A total of 55 nasal swab samples were collected from the cattle and buffaloes exhibiting the clinical signs of respiratory disease. All samples were tested for the detection of all four viral antigens by commercially available sandwich ELISA kit. BoHV-1 and BPI3V antigen were detected in 1 and 3 samples with the percentage of 1.8 and 5.4, respectively, whereas none of the sample was found positive for BRSV and BVDV antigen. The present study concluded the existence of BoHV-1 and BPI3V infection in respiratory tract and plays the important role in the respiratory viral infections of bovines. This study suggests the prevention measures should be taken to control the economic losses due to respiratory diseases in cattle and buffaloes.

  1. Abdallah, A. F. M. (2005). Studies on Respiratory syncytial virus in cattle at Sharkia Governorate. MVSc Thesis, Zagazig University, Egypt.

  2. Avci, O., Yavru, S. and Ekik. (2014). Detection of respiratory viral antigens in cattle lung tissues by direct ELISA. Animal and Veterinary Sciences. 5: 146-149.

  3. Bedekovic, T., Lemo, N., Barbic, L., Cvetnic, Z., Lojkic, I., Benic, M., Cac, Z., Lojkic, M. and Madic, J. (2013). Influence of category, herd size, grazing and management on epidemiology of bovine viral diarrhoea in dairy herds. Acta Veterinaria Brno. 82: 125-130.

  4. Collins, J. K., Ayers, V. K. and Carman, J. (1988). Evaluation of an antigen capture ELISA for the detection of bovine herpesvirus type 1 shedding from feedlot cattle. Veterinary Microbiology. 2: 101-107.

  5. Edwards, S., Chasey, D. and White, H. (1985). Experimental infectious bovine rhinotracheitis: comparison of four antigen detection methods. Research in Veterinary science. 1: 42-45. 

  6. Ellis, J., West. K., Konoby, C., Leard, T., Gallo, G., Conlon, J. and Fitzgerald, N. (2001). Efficacy of an inactivated respiratory syncytial virus vaccine in calves. Journal American Veterinary Medicine Association. 218:1973–1980.

  7. Fulton, R. W. and Confer, A. W. (2012). Laboratory test description for bovine respiratory diagnosis and their strengths and weaknesses: gold standards for diagnosis, do they exist? Canadian Veterinary Journal. 7: 754-761.

  8. Goswami, P. (2016). Pathoepidemology of important common pathogens of Bovine in Punjab. Doctoral Thesis. Guru Angad Dev Veterinary and Animal Science University, Ludhiana pp 80-82.

  9. Goswami, P., Banga, H. S., Deshmukh, S., Singh, N. D., Mahajan, V. and Brar, R. S. (2016). Sero-prevalence of bovine respiratory syncytial virus in bovine in Punjab, North India. Journal of Animal Research. 1: 67-73. 

  10. Gupta, V., Mishra, N., Rajukumar, K., Behera, S. P. and Dubey, P. (2014). Detection of bovine viral diarrhoea virus (BVDV) in peripheral blood mononuclear cells (PBMCs) of BVDV antibody positive Indian cattle following mitogen stimulation. Indian Journal of Animal Science. 84: 156-158.

  11. Hagglund, S., Svensson, C., Emanuelson, U., Valarcher, J. F. and Alenius, S. (2006). Dynamics of virus infections involved in the bovine respiratory disease complex in Swedish dairy herds. Veterinary Journal. 172: 320–328.

  12. Hazari, S., Panda, H. K., Kar, B. C. and Das, B. R. (2002). Comparative evaluation of indirect and sandwich ELISA for the detection of antibodies to bovine respiratory syncytial virus (BRSV) in dairy cattle. Comparative Immunology, Microbiology and Infectious Diseases. 25: 29-68.

  13. Houe, H. (2003). Economic impact of BVDV infection in dairies. Biological. 31: 137.

  14. Ibrahim, M. I., Desawy, O. M., Ali, N. O. and Nabi, S. S. (2008). Detection and isolation of bovine viral diarrhea in cattle by using different virological methods( Egypt. Journal of ComparativePathology and Clinical Pathology. 3: 148 – 160.

  15. Iglseder, A., Franz, S., Benetka, V., Mostl, K., Latif, M., Walk, K. and Baumgartner, W. (2011). Diagnosis of bovine viral respiratory diseases. Schweiz Arch Tierheilkd. 10: 441-447.

  16. Iscan, U. T. and Duman, R. (2011). Bovine Herpesvirus Type 1 (BHV-1) Prevalance in Dairy Cattle. Journal of Animal and Veterinary Advances. 12: 1523-1525.

  17. Klima, C. L., Zaheer, R., Booker, C.W., Hendrick, S., Alexander, T. W. and McAllister, T. A. (2014). Pathogens of bovine respiratory disease in North American feedlots conferring multidrug resistance via integrative conjugative elements. Journal of Clinical Microbiology. 2: 438-448.

  18. Majumdar, S., Ramakrishnan, M. A. and Nandi, S. (2015). Infectious Bovine Rhinotracheitis: An Indian Perspective. International Journal of Current Microbiology and Applied Sciences.10: 844-858.

  19. Masot, A.J., Gomez, T.C., Tovar, I., Gazquez, A. and Redondo, E. (1993). Location of bovine respiratory syncytial virus antigens in the lung of experimentally infected lambs: comparative study using indirect fluorescent antibody test, avidine biotin peroxidase complex and transmission electron microscopy. European Journal of Histochemistry. 37: 75-82. 

  20. Noori, Y. M., Intisar, K. S., Nasa, E. M. and Ali, Y. H. (2014). Detection and isolation of bovine Pparainfluenza virus Type 3 (PIV-3) in cattle in North Kordofan State, Sudan. Journal of Science and Technology. 15: 70-78.

  21. Office Internationale Des Epizootics (2008). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. OIE, Paris, France. 1343.

  22. Park, H. J., Kim, G. D., Lee, C. H., Kang M. H. and Min, K. S. (2016). Analysis of bovine viral diarrhea virus in Korea dairy farms. Indian Journal of Animal Research. 50: 224-229. 

  23. Patil, S. S., Prajapati, A., Krishnamoorthy, P., Desai, G. S., Reddy, G. B. M., Suresh, K. P. and Rahman, H. (2017). Seroprevalence of infectious bovine rhinotracheitis in organized dairy farms of India. Indian Journal of Animal Research. 51: 151-154.

  24. Percivalle, E., Zavattoni, M., Revello, M. G., Mariani, G., Scaravelli, C. and Gerna, G. (1989). Rapid detection of respiratory syncytial virus in nasopharyngeal secretions by immunofluorescence and ELISA does not justify discontinuation of virus isolation. Microbiologica. 2: 203-213.

  25. Puneet, V., Qureshi, S. D., Bhatia, A. K., Pradhan, A. K, Mishra N and Bhatia S. (2012). Molecular and antibody based diagnosis of bovine viral diarrhea virus (BVDV) associated with cattle from Mathura, Utter Pradesh India. Applied Biological Research. 14: 215-19.

  26. Quinting, B., Robert, B., Letellier, V.,Boxus, M., Kerkhofs, P., Schynts, F. and Collard, A. (2007). Development of a 1-step enzyme linked immunosorbent assay for the rapid diagnosis of bovine respiratory syncytial virus in postmortem specimens. Journal of Veterinary Diagnostic Investigantion. 3: 238-43.

  27. Ranganatha, S., Rathnamma, D., Patil, S. S., Chandranaik, B. M., Isloor, S., Veeregowda, B. M., Narayanabhat, M. and Srikala (2013). Isolation and molecular characterization of Bovine Herpes Virus-1 by Polymerase Chain Reaction. Indian Journal of Animal Research. 47(4): 340-43.

  28. Saber, M.S., Salem, A.A., Mohamed, S.A.H., Hadia, A.H.H., Nawal, A.M., Mossa, A.A. and Fatheia, M.M. (1996). Isolation, identification and seroconversion studies on BRSV in Egypt. Veterinary Medical Journal Giza. 44: 735- 747. 

  29. Saeed, I. K., Ali, Y. H., Taha, K. M., Mohammed, N. E., Noori, Y. M., Mohamed, B. A., Mohammad, O. I., Elmagboul, S. B. and Ghazali, F. A. 2016. Parainfluenza virus 3 infection in cattle and small ruminants in Sudan. Journal of Advanced Veterinary and Animal Research. 3: 236-41. 

  30. Selvaraj, J., Murali,M. B., Balachandran, C., Mishra, N., Pradhan, H.K. (2007). Seroprevalence of bovine viral diarrhoea in buffaloes at Chennai. Indian Journal of Veterinary Pathology. 31: 180.

  31. Singh, R., Verma, A.K., Sharma, B. and Yadav, S. K. (2013). Detection of bovine herpes virus-1 (BHV-1) infection in cattle by antigen detection ELISA and multiplex PCR. Advances in Animal and Veterinary Science. 3S: 12-16.

  32. Solis-Calderon, J. J., Segura-Correa, V. M., Segura-Correa, J. C. and Alvarado-Islas, A. (2003). Seroprevalence of and risk factors for infectious bovine rhinotracheitis in beef cattle herds of Yucatan, Mexico. Preventive Veterinary Medicine. 57: 199-208.

  33. Urban-Chmiel, R., Wernicki, A., Puchalski, A., Stegierska, D., Grooms, D. L. and Barbu, N. I. (2013). Rapid detection of bovine respiratory syncytial virus in Poland using a human patient side diagnostic assay. Transboundry and Emerging Diseases. 62: 407-10.

  34. Van der Poel, W.H., Brand, A., Kramps, A. and van Oirschot, J. T. (1994). Respiratory syncytial virus infections in human beings and in cattle. Journal of Infectious Disease. 29: 215–228.

  35. Woodbine, K. A., Medley, G. F., Moore, S. J., Ramirez-Villaescusa, A. M., Mason, S. and Green, L. E. (2009). A fouryear longitudinal seroepidemiological study of bovine herpsvirus type -1 (BHV-1) in adult cattle in 107 unvaccinated herds in south-west England. BMC Veterinary Research. 5: 5-5.

  36. Yavru, S., Simsek, A., Yaplico, O. and Kale, M. (2005). Serological evaluation of viral infection in bovine respiratory tract. Acta Veterinary. 2: 219-26. 

Editorial Board

View all (0)