Asian Journal of Dairy and Food Research, volume 33 issue 1 (march 2014) : 1-4

CHARACTERIZATION OF PIGMENT PRODUCING RHODOTORULA FROM DAIRY ENVIRONMENTAL SAMPLES

Shivalkar Yadav*, K.H. Manjunatha, B. Ramachandra, N. Suchitra, R. Prabha
1Department of Dairy Microbiology, Dairy Science College, KVAFSU, Hebbal, Bengaluru-560 024, India
Cite article:- Yadav* Shivalkar, Manjunatha K.H., Ramachandra B., Suchitra N., Prabha R. (2024). CHARACTERIZATION OF PIGMENT PRODUCING RHODOTORULA FROM DAIRY ENVIRONMENTAL SAMPLES. Asian Journal of Dairy and Food Research. 33(1): 1-4. doi: 10.5958/j.0976-0563.33.1.001.
Microbial pigments due to their higher biodegradability and higher compatibility with the environment, offer promising avenues for various applications in food, pharmaceuticals, cosmetics and textiles. Yeast is a eukaryotic organism and among the yeast, species of genus Rhodotroula produces many kinds of carotenoid pigments. The present study was taken to isolate and identify the pigment producing Rhodotorula species from dairy environmental samples. Air sample showed highest count (3.47 log cfu/min) followed by apple and can milk. Rhodotorula isolates of 11 were obtained from air (3), apple (4), can milk (2) and yoghurt (2). Identification of the isolates revealed 3 as Rhodotorula minuta, while 2 as Rhodotorula acheniorum and 6 as unidentified isolates as they did not match with identification key. All the isolates of Rhodotorula were screened for pigment production based on urea hydrolysis Rhodotorula minuta RAI3; Rhodotorula acheniorum RC2 ,Rhodotorula sp RA2, Rhodotorula minuta RY1 were selected for pigment production.
  1. Ahmad, W.A., Ahmad, w.y.w., Zakaria, Z.A., and YUSOF, N.Z. (2012). Application of Bacterial Pigments as Colorants: The Malaysian Perspective. Springer, New York.
  2. Aberoumand, A. (2011).A review article on edible pigments properties and sources as natural biocolorants in foodstuff and food industry. World J. Dairy.Food Sci., 6: 71-78.
  3. Costa HL, Martelli DA Silvaim and Pomeroy D. (2005).Production of b-carotene by a Rhodotorula strain. Biotechnollett., 9: 373-375.
  4. Dufosse, L. (2006).Microbial production of food grade pigments. Food Technol. Biotechnol, 44: 313-321.
  5. Harrigan. (1998).Laboratory methods in food and dairy microbiology. Department of Food Science, Reading University, Academic Press Inc. (London) Ltd. UK
  6. Iriani R.M.,R.P.Adilma, scamparini, B Delia, A. Rodriguez (2005).Selection and characterization of carotenoid-producing yeasts from Campinas region. Braz J. Microbiol., 40: 2985-2991.
  7. Joshi, V.K., D. Attri, and Rana, N.S. (2011).Optimization of apple pomace based medium and fermentation conditions for pigment production by Sarcina sp..Indian J. Natural Product. Res. 2: 421-427.
  8. Kaur, B., D. Chakraborty and H. Kaur. (2009). Production and stability analysis of yellowish pink pigments from Rhodotorula rubra MTCC 1446. Internet J. Microbiol., 7: 1.
  9. Seeliger, Heinz P. R., (1955). Use of a urease test for the screening and identification of Cryptococci.Mortensen, a. (2006).Carotenoids and other pigments as natural colorants. Pure. Appl. Chem. 78: 1477-1491.
  10. Mohanasrinivasan, V., Sriram Kalyan, P., Ipsita, N., Subathradevi, C., Selvarajan, E., Suganthi, V., and Jemimah Naine, S. (2013).Fermentative production of extracellular pigment from Streptomyces coelicolor MSIS1. Res. J. Biotechnol. 8: 31-41.
  11. Natarajan AM. (2007).Development of low calorie rasogolla fortified with effective functional ingredients with therapeutic value. Heath Foods, 41-42.
  12. Postgate J. (1994). “The Outer Reaches of Life”, Cambridge University Press, 132-134, Wikipedia free encyclopedia. Stafsnes MH, dybwad M, Brunsvik A, Bruheim P. (2013). Large scale maldi-tof ms based taxa identification to identify novel pigment producers in a marine bacterial culture collection. Antonie Van Leeuwenhoek 103(3): 603-15.
  13. Suntroshuk W. (2004).Synthesis of carotenoids by Rhodotorula rubra GED8 co-cultured with yogurt starter cultures in whey ultrafiltrate. J.Industrial. Microbiol .Biotechnol., 115-121.
  14. Tinoi J, Rakariyatham N, Deming RL. (2005). Simplex optimization of carotenoid production by Rhodotorula glutinis using hydrolyzed mung bean waste flour as substrate. Process Biochem., 40: 2551–2557.
  15. Varsha, C., and Arpana, J. (2013).Screening of significant nutrient parameters for pigment production from newly isolated organism Planococcus maritimus AHJ_2 using Plackett- Burman design. J. Microbiol. Biotechnol. Res.3: 79-83.

Editorial Board

View all (0)