Asian Journal of Dairy and Food Research, volume 33 issue 4 (december 2014) : 311-314

INTESTINAL ABSORPTION OF NUTRIENTS USING RATS FED DIFFERENT DIETS

D.R. Sood1, S.C. Deka, Rajat Sood2, Alka Sharma3

Affiliations

¹Department of Biochemistry, CCS Haryana Agricultural University, Hisar-125 004, India

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First Online |
doi 10.5958/0976-0563.2014.00623.X

Cite article:- Sood1 D.R., Deka S.C., Sood2 Rajat, Sharma3 Alka (2024). INTESTINAL ABSORPTION OF NUTRIENTS USING RATS FED DIFFERENT DIETS. Asian Journal of Dairy and Food Research. 33(4): 311-314. doi: 10.5958/0976-0563.2014.00623.X.

ABSTRACT

Absorption of glucose, sucrose, glutamic acid, methionine, lysine and cysteine was studied using the intestinal everted sac technique. Rats were fed rice diets Basmati 370, Pusa Basmati 1 and Haryana Basmati 1 using as a casein control. Absorption of nutrients was monitored spectrophotometrically and of glucose, sucrose and glutamic acid by 14c radio assay using scintillation counting. Positive correlation coefficients between the two assay methods for glucose (r = 0.84), sucrose (r = 0.38) and glutamic acid (r = 0.80) were observed. Glucose showed higher absorption than sucrose and glutamic acid than lysine, cysteine and methionine. Absorption of glucose, sucrose and glutamic acid were higher in rats fed on Haryana Basmati 1 diet as demonstrated by both the methods. Rats on Basmati 370 diet exhibited higher methionine, lysine and cysteine absorption. Absorption of nutrients differed significantly between casein and rice.

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REFERENCES

Akedo, H.; Sugava, T.; Yosikawa, S. and Suda, M. (1960). Absorption of glucose and other compounds to the peroxide oil fed rat. J. Biochem., 47: 124.
Brune, M.; Rossauder, L. and Hallberg, L. (1989). Iron absorption and phenolic compounds; importance of different phenolic structures. Eur. J. Clin. Nutr., 43: 547-558.
Fong, J.; Schaffer, P.L. and Kirk, P.K. (1953). Absorption pattern of different nutrients with respective to standard diet. Arch Biochem Biophys, 45: 319.
HAU (1981). Package of Practices for Kharif Crops. Haryana Agricultural University, Hisar, India.
Ibrahim, S.A. and Balasubramanian, K.A. (1995). Lipid composition and membrane fluidity of monkey small intestine brush border membrane: Regional differences. Ind. J. Biochem. Biophys., 32: 290-294.
Kaur, P. (1988). Biochemical and physiological effects of feeding peroxidized oil in albino rats. Ph.D. Thesis, Punjab Agricultural University, Ludhiana, India.
Kohli, K.K.; Sharma, S.C.; Bharta, S.C. and Venketasubramaniam (1975). Biochemical effects of chlorinated insecticides DDT and dieldrin. J. Indus Res., 34: 462.
Mahmood, A.; Agarwal, B.; Sanyal, S. and Subrahmanyan, D. (1978). Effect of DDT administration on glucose uptake and brush border enzymes in monkey intestine. Acta Pharmac. Tox., 43: 29.
Majumdar, S. and Panda, J.N. (1994). Intestinal glucose absorption capabilities of high, medium and non-laying birds. India. J. Ani. Sci., 69: 1165-1168.
Miller, D. and Crank, R.K. (1961). The digestive function of the epithelium of small intestine 1. An intracellular locus of disaccharide and sugar phosphate ester hydrolysis. Biochem. Biphys Acta, 52: 281-293.
Raghuramulu, N.; Madhavan Nair, K. and Kalyamasundaram, S. (1983). A manual of laboratory techniques, NIN, Indian Council of Medical Research, Hyderabad, India.
Rama Rao, A.V.S.S. (1991). Text book of Biochemistry for Medical Students. 6th edn. L.K. and S Publishers, Visakhapatnam, India. P 167.
Singh, J.; Sood, D.R.; Galhotra, M.M. and Sharma, S.K. (1996). Comparison of two methods for determining in vitro intestinal absorption of nutrients using rats fed different diets. Plant Foods for Human Nutrition, 49: 235-240.
Suneja, S.K.; Ram, G.C. and Wagle, D.S. (1984). Effects of T-2 toxin on glucose and tryptophan uptake and intestinal mucosal enzymes. Toxicon, 22: 39.
Talwar, G.P.; Srivastava, L.M. and Moudgil, K.D. (1989). Text book of biochemistry and Human Biology. 2nd edn., Prentice Hall of India Pvt. Ltd., New Delhi, India.
Yemn, E.W. and Cocking, F.C. (1955). The determination of amino acid with ninhydrin. Analyst, 80: 203-213.

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Asian Journal of Dairy and Food Research

INTESTINAL ABSORPTION OF NUTRIENTS USING RATS FED DIFFERENT DIETS

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