Asian Journal of Dairy and Food Research, volume 41 issue 4 (december 2022) : 450-455

​Evaluation of Essential Oils as a Hurdle for Ambient Temperature Storage of Chicken Patties

A. Kalaikannan, A. Elango, J. Johnson Rajeshwar, D. Santhi
1Regional Research and Educational Centre, Pudukkottai-622 004, Tamil Nadu, India.
Cite article:- Kalaikannan A., Elango A., Rajeshwar Johnson J., Santhi D. (2022). ​Evaluation of Essential Oils as a Hurdle for Ambient Temperature Storage of Chicken Patties. Asian Journal of Dairy and Food Research. 41(4): 450-455. doi: 10.18805/ajdfr.DR-1844.
Background: Clove bud oil (CBO) and oleoresin rosemary (ORM) possess antioxidant and antibacterial properties and are widely used as natural preservatives in meat products. The present study was conducted with the objective of evaluating the essential oils CBO and ORM as a hurdle for extending the shelf life of chicken patties at ambient temperature storage.
Methods: The natural preservatives viz. CBO at 0.05% level (T1) and ORM at 0.1% level (T2) and their combination (CBO 0.05% with ORM 0.1%) (T3) were incorporated in chicken meat patties in the basic formulation along with a control (C) and the cooked product quality packed in food grade pet-poly co-extruded film pouches stored at ambient temperature (30±2°C) was assessed. 
Result: Upon storage, in terms of sensory evaluation, T3 spoiled on 5th day and other samples on 3rd day of storage. Addition of essential oils significantly (P<0.05) enhanced the DPPH scavenging effect. TBARS and tyrosine values of all treatments significantly (P<0.05) increased linearly with storage period. CBO and ORM combination significantly (P<0.05) inhibited the microbial load during the storage period and maintained the odour score in the acceptable level even at 3rd day of storage. The combination of CBO and ORM might be applied as one of the hurdles in adopting the ‘Hurdle Technology’ storage of chicken meat patties. 

  1. Al-Hijazeen, M. and Al-Rawashdeh, M. (2019). Preservative effects of rosemary extract (Rosmarinus officinalis L.) on quality and storage stability of chicken meat patties. Food Science and Technology. 39: 27-34.

  2. AOAC. (1975). Official Methods of Analysis. 12th ed., Association of Official Analytical Chemists, Washington DC.

  3. Aruoma, O.I., Halliwell, B., Aeschbach, R. and Loligers, J. (1992). Antioxidant and pro-oxidant properties of active rosemary constituents: carnosol and carnosic acid. Xenobiotica. 22: 257-268.

  4. Babu, A.J., Sravanth, M.S., Reddy, D.M., Karthikeyan B. and Rupasundari, A. (2012). Studies on the effect of essential oils of spices on physico-chemical, microbial and organoleptic properties of chicken meat patties during refrigerated storage. Journal of Advanced Veterinary Research. 2: 127-136.

  5. Ibrahim, H., Hassan, M. Amin, R. Shawqy, N. and Elkoly, R. (2018). Effect of Some Essential Oils on the Bacteriological Quality of Some Chicken Meat Products. Benha Veterinary Medical Journal. 35: 42-49.

  6. ICMSF (1978). International Commission on Microbiological Specifications for Foods: Microorganisms in Foods. Sampling for microbial analysis: Principles and Specific Applications. University of Toronto Press, Canada. 

  7. Jaworska, D., Rosiak, E., Kostyra, E., Jaszczyk, K., Wroniszewska, M., Przybylski, W. (2021). Effect of herbal addition on the microbiological, oxidative stability and sensory quality of minced poultry meat. Foods. 10: 1537. 

  8. Kalaikannan, A., Anjaneyulu A.S.R. and Santhi, D. (2007). Effect of egg proteins on the quality and refrigerated storage life of chicken patties made with broiler-spent hen meat and by-products. International Journal of Food Science and Technology. 42: 579-586.

  9. Karthikeyan, J., Kumar, S. Anjaneyulu A.S.R. and Rao, K.H. (2000). Application of hurdle technology for the development of Caprine keema and its stability at ambient temperature. Meat Science. 54: 9-15.

  10. Keeton, J.T. (1983). Effects of fat and NaCl/phosphate levels on the chemical and sensory properties of pork patties. Journal of Food Science. 48: 878-881.

  11. Leistner, L. (2000). Basic aspects of food preservation by hurdle technology. International Journal of Food Microbiology. 55: 181-186.

  12. Michalczyk, M., Macura, R. Tesarowicz I. and Banas, J. (2012). Effect of adding essential oils of coriander (Coriandrum sativum) and hyssop (Hyssopus officinalis) on the shelf life of ground beef. Meat Science. 90: 842-850.

  13. Mielnik, M.B., Sem, S. Egelandsdal B. and Skrede, G. (2008). By- products from herbs essential oil production as ingredient in marinade for turkey thighs. LWT - Food Science and Technology. 41: 93-100.

  14. Modi, V.K., Yashoda, K.P. Bhaskar N. and Mahendrakar, N.S. (2009). Effect of carrageenan and oat flour on storage characteristics of fried mutton kofta. Journal of Food Processing and Preservation. 33: 763-776.

  15. Mukhtar, S., Zahoor, T. Randhawa, M.A. Iqbal R. and Shabbir, A. (2018). Synergistic Effect of Chitosan and Clove Oil on Raw Poultry Meat. Journal of Food Processing and Technology. 9: 737-741.



  16. Nychas, G.J.E. (1995). Natural antimicrobials from plants. In New methods of food preservation (pp. 58-89). Springer US.

  17. Qin, Y., Li, W., Liu, D., Yuan, M., and Li, L. (2017). Development of active packaging film made from poly (lactic acid) incorporated essential oil. Progress in Organic Coatings. 103: 76-82.

  18. Saricaoglu, F.T. and Turhan, S. (2019). Performance of mechanically deboned chicken meat protein coatings containing thyme or clove essential oil for storage quality improvement of beef sucuks. Meat science. 158:107912 https://doi.org/ 10.1016/j.meatsci.2019.107912.

  19. Sarýcaoglu, F.T. and Turhan, S. (2020). Physicochemical, antioxidant and antimicrobial properties of mechanically deboned chicken meat protein films enriched with various essential oils. Food Packaging and Shelf Life. 25: 100527.

  20. Sharma, H., Mendiratta, S.K., Agarwal, R.K., and Gurunathan, K. (2020). Bio-preservative effect of blends of essential oils: natural anti-oxidant and anti-microbial agents for the shelf life enhancement of emulsion based chicken sausages.  Journal of Food Science and Technology. 57: 3040-3050.

  21. Snedecor, G.W. and Cochran, W.G. (1995). Statistical Methods (8th ed.). New Delhi: Oxford and IBH Pub. Co.

  22. Strange, E.D., Benedict, R.C. Smith J.L. and Swift. C.E. (1977). Evaluation of rapid tests for monitoring alterations in meat quality during storage. Journal of Food Protection. 40: 843-847.

  23. Sureshkumar, S., Kalaikannan, A. Dushyanthan K. and Venkataramanujam, V. (2010). Effect of nisin and butylated hydroxy anisole on storage stability of buffalo meat sausage. Journal of Food Science and Technology. 47: 358-363.

  24. Tarladgis, B.G., Watts, B.M. Younathan M.T. and Dugan Jr, L. (1960). A distillation method for the quantitative determination of malonaldehyde in rancid foods. Journal of the American Oil Chemists’ Society. 37: 44-48.

  25. Thomas, R., Anjaneyulu A.S.R. and Kondaiah, N. (2008). Development of shelf stable pork sausages using hurdle technology and their quality at ambient temperature (37±1oC) storage.  Meat Science. 79: 1-12.

  26. Vergara, H., Cózar, A., and Rubio, N. (2021). Lamb meat burgers shelf life: effect of the addition of different forms of rosemary (Rosmarinus Officinalis L.). CyTA-Journal of Food, 19: 606-613.

  27. Wu, H. C., Chen H.M. and Shiau, C.Y. (2003). Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus). Food Research International. 36: 949-957

  28. Xu, T., Gao, C., Feng, X., Huang, M., Yang, Y., Shen, X. and Tang, X. (2019). Cinnamon and clove essential oils to improve physical, thermal and antimicrobial properties of chitosan- gum arabic polyelectrolyte complexed films. Carbohydrate Polymers. 217: 116-125.

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