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Asian Journal of Dairy and Food Research, volume 38 issue 2 (june 2019) : 87-92

Comparative Study Between Conventional Preservatives and Methylene Blue on Chemical and Bacteriological Composition of Raw Milk

Y Hachana, A Rebhi
1Department of Animal Production, High Agronomic Institute of Chott-Meriam, Susa, Susa Tunisia
Cite article:- Hachana Y, Rebhi A (2019). Comparative Study Between Conventional Preservatives and Methylene Blue on Chemical and Bacteriological Composition of Raw Milk. Asian Journal of Dairy and Food Research. 38(2): 87-92. doi: 10.18805/ajdfr.DR-129.

ABSTRACT

The objectives of this study were to compare conventional preservatives used in milk analysis, including potassium dichromate and bronopol with a non-toxic and biodegradable preservative such as methylene blue, to verify their effectiveness and limitations of use. This was achieved by comparing their effects on raw milk composition at different storage temperatures. The best performing preservative doses were 0.1, 0.036 and 0.01% for potassium dichromate, bronopol, and methylene blue respectively. Raw milk composition was significantly affected (p < 0.05) during the storage period at 25°C for all tested preservatives. However, at 4°C, potassium dichromate and bronopol preservatives maintained stable milk composition during 10 days of storage, whereas methylene blue maintained stable milk composition for three days. Methylene blue can be considered as an environmentally friendly and safe alternative for short-term preservation of refrigerated raw milk samples.

REFERENCES

  1. Barbano, D.M., Wojciechowski, K.L. and Lynch, J.M. (2010). Effect of preservatives on the accuracy of mid-infrared milk component testing. Journal of Dairy Science. 93: 6000-6011.
  2. Benda P (1995). The effect of some preservatives on natural microûora in milk samples. Veterinary Medicine Czhech. 40: 359–364.
  3. Bertrand J.A (1996). Influence of shipping container, preservative and breed on analysis of milk components of shipped samples. Journal of Dairy Science. 79:1-148.
  4. Bumbadiya, M., Singh, R., Pradhan, D., Mann, B. and Arora, S. (2017). Screening of different novel preservatives for milk Preservation by microbial analysis. International Journal of Chemical Studies. 5: 673-677. 
  5. Chalermsan, N., Vijchulata, P., Chirattanayuth, P., Sintuwanit, S., Surapat, S. and Engkagul, A. (2004). Effects of preservatives on raw milk components analyzed by infrared spectrophotometry. Kasetsart Journal Natural Science. 38: 38-43.
  6. Chang, S. and Lamm, S.H. (2003). Human Health Effects of Sodium Azide Exposure: A Literature Review and Analysis. International Journal of Toxicology. 22:175-86.
  7. Grappin, R. and Jeunet, R. (1970). The Milko-Tester automatic for routine determination of fat in milk. Le Lait. 50: 233–256.
  8. Hamann, J. and Zecconi, A. (1998). Evaluation of the electrical conductivity of milk as a mastitis indicator. Bulletin of the IDF. 334: 26 p.
  9. ISO 4833-1 (2013). Microbiologie de la chaîne alimentaire. Méthode horizontale pour le dénombrement des micro-organismes. Partie 1: Comptage des colonies à 30 degrés C par la technique d’ensemencement en profondeur (in French).
  10. ISO 5764 (2009). Lait. Détermination du point de congélation. Méthode au cryoscope à thermistance. Méthode de référence (in French).
  11. ISO 9622 (2013). Lait et produits laitiers liquids. Lignes directrices pour l’application de la spectrométrie dans le moyen infrarouge (in French).
  12. Kroger M (1985). Milk sample preservation. Journal of Dairy Science. 68: 783–787. 
  13. Mabon, R.M and Brechany, E.Y. (1983). The measurement of urea in fresh and stored goats milk. Laboratory Practicls. 32: 81.
  14. Marina, N.U., Matthew, C.T. and Merrill, A.B. (2001). Comparison of the methylene blue and toluidine blue photobactericidal efficacy against gram positive and gram negative microorganisms. Laser Surgery and Medicine. 29: 165-173.
  15. Monardes, H.G., Moore, R.K., Corrigan, B. and Rioux, Y. (1995). Preservation and Storage Mechanisms for Raw Milk Samples for Use in Milk-Recording Schemes. Journal of Food Protection. 59:151-154.
  16. New Jersey Department of Health (2010). Hazardous Substance Fact Sheet. http://www.nj.gov/health/eoh/rtkweb.
  17. Ng-Kwai-Hang, K.F. and Hayes, J.F. (1982). Effects of potassium dichromate and sample storage time on fat and protein by milko-    scan and on protein and casein by a modified pro-Milk Mk II method. Journal of Dairy Science. 65:1895-1899.
  18. Oz, M., Lorke, D.E., Hasan, M. and Petroianu, G.A. (2011). Cellular and molecular actions of Methylene Blue in the nervous system. Medicinal Research Reviews. 31: 93–117.
  19. Sanchez, A., Sierra, D., Luengo, C., Corrales, J.C., Morales, C.T., Contreras, A. and Gonzalo, C. (2005). Influence of Storage and Preservation on Fossomatic Cell Count and Composition of Goat Milk. Journal of Dairy Science 88: 3095–3100.
  20. Sesh, P.S.L., Loganathasamy, K. and Ayyadurai, K. (2012). A study on the chemical composition of ass’s and camel’s milk. Journal of dairying, foods & home sciences. 31: 81 – 84.
  21. Upadhyay, N., Goyal, A., Kumar, A., Ghai, D.L. and Singh, R. (2014). Preservation of milk and milk products for analytical purposes. Food Reviews International. 30: 203–224.
  22. Wainwright, M. and Crossley, K.B. (2002). Methylene blue a therapeutic dye for all seasons. Journal of 1Chemotherapy. 14: 431-443. 
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