Phytosterol and its esters as novel food ingredients: A review

ABSTRACT

Phytosterols or stanols are large group of compounds found exclusively in plants. These are naturally present in plants and are structurally similar to cholesterol. A daily intake of 3 g of phytosterol (or their reduced form stanols) is associated with consistent and reproducible reduction in LDL cholesterol concentrations upto 10% and reduces the risk of coronary heart disease by 20% over a lifetime. Studies have concluded that the effective doses for reduction of cholesterol are between 1.5 and 3g/day, leading to decrease in 8% and 15% of LDL-cholesterol. The principal mechanism of action is based on interference with the solubilization of the cholesterol in the intestinal mucosa. Several studies have validated the cholesterol lowering effect of phytosterols and stanols along with its role in prevention of coronary heart diseases. Plant sterols and stanols appear to be unhazardous to health in a large number of human studies and no evidence of toxicity even at high dose levels has been reported. Changing lifestyle, constant stress and risk towards various diseases have necessitated the need of such vital neutraceuticals to be incorporated in diet and daily food items. Such new foods and formulations should pave the way for greater use of phytosterols in heart health promotion, increasing the long term potential for the creation of innovative functional foods containing plant sterols and their derivatives.

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REFERENCES

Amundsen A L, Ose L, Nenseter M S and Ntanios F Y (2002). Plant sterol ester enriched spread lowers plasma total and LDL cholesterol in children with Familial Hypercholesterolemia. Am. J. Clin Nutr 76:338-44.

Amundsen A L, Ose L, Ntanios F Y (2001). Effects of plant sterol ester enriched spread on plasma lipids and safety parameters in children with Familial Hypercholesterolemia (FH) in controlled and follow up periods. Abstract in Annals of nutrition and metabolism, 45(Supplement1), 125(Proceeding of 17th International Congress of Nutrition), August 27-30, 2001, Vienna, Austria.

Anon (2000). Phytosterols and the Cancer Connection. Cited on: Sept’ 2010. http://www.peanut-institute.org/resources/ downloads/fft_v4i3.pdf.

Anon (2006). Phytosterols supplements side effects and benefits, role in cholesterol metabolism and prostate health by Ray Sahelian, M.D. Cited on: Aug’2009. http://www.raysahelian.com/phytosterols.html.

Anon (2007). India has one of the highest cancer rates in the world. Cited on: April 2009. https://nitawriter.wordpress.com/ 2007/04/26/india-has-one-of-the-highest-cancer-rates-in-the-world/

Anon (2008). Phytosterols and Stanols. Cited on: Aug’ 2010. http://www4.agr.gc.ca/resources/prod/doc/misb/fb-ba/nutra/ pdf/phytosterols_stanols_eng.pdf.

Anon (2010). Food Labeling; Health Claim; Phytosterols and Risk of Coronary Heart Disease A Proposed Rule by the Food and Drug Administration. (http://www.federalregister.gov/articles/2010/12/08/2010-30386/food-labeling- health-claim-phytosterols-and-risk-of-coronary-heart-disease#h-17) Assessed on 12/08/2010.

Anon (2011a). Phytosterols, phytostanols and their esters. Accessed on 22 May’2009. http://www.fao.org/ag/agn/jecfa- additives/specs/monograph5/additive-509-m5.pdf.

Anon (2011b). Cholesterol. http://en.wikipedia.org/wiki/Cholesterol.

Anon (2012a). Lower your serum cholesterol with plant sterols by Dr Harold Gunatillake. http://www.thesundayleader.lk/ 2012/02/26/lower-your-serum-cholesterol-with-plant-sterols/.

Anon (2012b). India’s no. 1 killer: Heart disease. (http://indiatoday.intoday.in/ story/ India’s +no.1+killer:+Heart+disease/ 1/92422.html. Assessed on 08/06/2012.

Australian Bureau of Statistics (2009). Causes of Death 2007 (3303.0). March.

Awad A B, Downie A, Fink C S and Kim U (2000a). Dietary Phytosterol Inhibits the Growth and Metastasis of MDA-MB-231 Human Breast Cancer Cells Grown In SCID Mice. Anticancer Res 20:821-824.

Awad A B and Fink C S (2000a). Phytosterols as anticancer dietary components: evidence and mechanism of action. J Nutr 130:2127-2130.

Awad A B, Karen C C, Downie A C and Fink C S (2000b). Peanuts as a Source of B-sitosterol, a Sterol with Anticancer Properties. Nutr and Cancer 36:238-241.

Baker V A, Hepburn P A, Kennedy S J, Jones P A, Lea L J, Sumpter J P and Ashby J (1999). Safety evaluation of phytosterol esters Part 1. Assessment of oestrogenicity using a combination of in vivo and in vitro methods. Food Chem Toxicol 37:13-22.

Berger A, Jones P J H and Abumweis S S (2004). Plant sterols: factors affecting their efficacy and safety as functional food ingredients. Lipids Health Dis, 3:1-54.

Carr T P, Cornelison R M, Illston B J, Stuefer-Powell C L and Gallaher D D (2001). Plant sterols alter bile acid metabolism and reduce cholesterols absorption in hamsters fed a beef-based diet. Nutr Res, 22:745-754.

Chen Q, Gruber H, Swist E, Coville K, Pakenham C, Ratnayake W M N and Scoggan K A (2010). Dietary phytosterols and phytostanols decrease cholesterol levels but increase blood pressure in WKY inbred rats in the absence of salt- loading. Nutr & Metabol 7:11-20.

Cherif A O, Trabelsi H, Ben Messaouda M, Kaabi B, Pellerin I, Boukhchina S, Kallel H and Pepe C (2010). Gas chromatography- mass spectrometry screening for phytochemical 4-desmethylsterols accumulated during development of Tunisian peanut kernels (Arachis hypogaea L.). J Agric Food Chem 58:8709-14.

Christiansen L I, Lahteenmaki P L A, Mannelin M R, Seppanen-Laakso T E, Hiltunen R V K and Yliruusi J K (2001). Cholesterol- lowering effect of spreads enriched with microcrystal line plant sterols in hypercholesterolemic subjects. Eur J Nutr 40:66-73.

Christie W W (2011). Sterols and their conjugates from plants and lower organisms: Structure, Occurrence, Biochemistry and Analysis. Cited on: 2nd Feb’2011. http://lipidlibrary.aocs.org/lipids/plant_st/index.htm.

Chryssafidis D, Maggos P, Kiosseoglou V and Boskou, D (1992). Composition of total and esterified 4a-monomethylsterols and triterpene alcolhols in virgin olive oil. J Sci Food Agric 58:581-586.

Clifton P (2002). Plant sterol and stanols-comparison and contrasts. Sterol versus stanols in cholesterol-lowering: is there a different? Atherosclerosis 3:5-9.

Davidson M H, Maki K C, Umporowicz D M, Ingram K A, Dicklin M R, Schaefer E, Lane R W, McNamara J R, Ribaya- Mercado J D, Perrone G, Robins S J and Franke W C (2001). Safety and tolerability of esterified phytosterols administered in reduced-fat spread and salad dressing to healthy adult men and women. J Am Coll Nutr 20:307-319.

Dutta P C and Appelqvist L A (1996). Saturated sterols (stanols) in unhydrogenated and hydrogenated edible vegetable oils and in cereal lipids. J Sci Food Agric 71:383-391.

Eldin K A and Appelqvist L A (1994). Variation in the composition of sterols, tocopherols and lignans in seed oils from four sesamum species. J Am Oil Chem Soc 71:149-156.

European Food Safety Agency (2003). Opinion of the scientific panel on dietetic products, nutrition and allergies on a request from the Commission related to a novel food application from Forbes Medi-Tech for approval of plant sterol-containing milk-based beverages. Request No. EFSA-Q-2003-075. EFSA J. 15:1-12.

Ferrari R A P, Schulte E, Esteves W, Bruhl L and Mukherjee K D (1996). Minor constituents of vegetable oils during industrial processing. J Am Oil Chem Soc 73:587-592.

Ferrari R A P, Esteves W, Mukherjee K D and Schulte E (1997). Alteration of sterols and steryl esters in vegetable oils during refining. J Agric Food Chem 45:4753-4757.

Geelen A, Zock P L, De Vries J H and Katan M B (2002). Apolipoprotein E polymorphism and serum lipid response to plant sterols in humans. Eur J Clin Invest. 32: 738–742.

Gordon M H and Griffith R E (1992). A comparison of steryl esters of coconut and palm kernel oils. Fat Sci Technol 94:218-221.

Gunstone, F D (2005). Vegetable Oils In: Bailey’s Industrial Oil and Fat Products, Sixth Edition, Volume 1. Edited by Fereidoon Shahidi., Wiley Interscience, A John Wiley & Sons, Inc., Publication pp: 213-242.

Hallikainen M A, Sarkkinen E S, Gylling H, Erkkila A T and Uustiupa M I J (2000a). Comparison of effects of plant

sterol esters and plant stanol esters – enriched margarines in lowering serum cholesterol concentration in hypocholesterolemic subjects on a low fat diet. Eur J Clin Nutr 54:715-725.

Hallikainen M A, Sarkkinen E S and Uusitupa M I J (2000b). Plant sterol esters affects serum cholesterol concentration of hypocholesterolemic men and women in a dose dependent manner. J Nutr 130:767-776.

Hallikainen M A and Uustiupa M I J (1999). Effects of 2 low fat stanols sterol conataing margarines on serum cholesterol concentrations as part of a low fat diet in hypercholesterolemic subjects. Am J Clin Nutr.41: 403-410.

Hendriks H F, Brink E J, Meijer G W, Princen H M and Ntanios F Y (2003). Safety of long-term consumption of plant sterol esters-enriched spread. Eur J Clin Nutr 57: 681–692.

Hendriks H F, Weststrate J A, Vliet V T and Meijer G W (1999). Spreads enriched with three different levels of vegetable oil sterols and the degree of cholesterol lowering in normocholesterolaemic and mildly hypercholesterolaemic subjects. Eur J Clin Nutr 53: 319–327.

Hepburn P A, Horner S A and Smith M (1999). Safety evaluation of phytosterol esters. Part 2. Subchronic 90-day oral toxicity study on phytosterol esters- a novel functional food. Food Chem Toxicol 37:521-532.

Hicks K B, Moreu R A (2001). Phytosterols and stanols: functional food cholesterol buster. Food Technol 55:63-67.

Hoffpauer D W and Wright S L (2002). Fortified rice bran food product and method for promoting cardiovascular health. US patent 6436431.

Homberg E and Bielefeld B (1989). Composition and content of sterols in 41 different vegetable and animal fats. Fat Sci. Technol 91:2127.

IFST (2005). Current hot topics: phytosterol esters (plant sterol and stanols esters). Institute of Food Science and Technology, 5 Cambridge Court, 210 Shepherd’s Bush Road, London W6 7NJ, UK.

Johansson K (1979). Sterols in vegetable oils: aspects of biological variation, influence of technological treatment, and the use of sterols to identify vegetable oils. Ph.D Thesis. Uppsala: Swedish University of Agricultural Sciences.

John S, Alexey V, Sorokin Paul and Thompson D (2007). Phytosterols and vascular disease. Curr Opin Lipidol 18:35–40.

Jones P J H J and Abu Mweis S S (2009). Phytosterols as functional food ingredients: linkages to cardiovascular disease and cancer. Current opinion in clinical nutrition and metabolic care 12:147-151.

Judd J T, Baer D J, Chen S C, Clevidence A B, Muesing R A, Kramer M and Meijer G W (2002). Plant sterols esters lower plasma lipids and most carotenoids in mildly hypercholesterolemic adults. Lipids 37:33-42.

Katan M B, Grundy S M and Jones P (2003). Efficacy and safety of plant stanols and sterols in the management of blood cholesterol levels. Mayo Clin Proc 78:965–978.

Kritchevsky D and Chen S C (2005). Phytosterols-health benefits and potential concerns: a review, Nutr Res 25: 413–428.

Law M (2000). Plant sterol and stanol margarines and health. Bri. Med. J. 320: 861–864.

Ling W H and Jones P J H (1995). Dietary phytosterols: a review of metabolism, benefits and side effects. Life Sci. 57:195- 206.

Mattson F H, Volenphein R A and Erickson B A (1997). Effect of plant sterol esters on the absorption of dietary cholesterol. J Nutr 107:1139-1146.

Matvienko O A, Lewis D S, Swanson M, Arndt B, Rainwater D L, Stewart J and Alekel D L (2002). A single daily dose of soybean phytosterols in ground beef decrease serum total cholesterol and LDL cholesterol in young, mildly hypercholesterolemic men. Am J Clin Nutr 76:57-64.

Miettinen T A, Puska P, Gylling H, Vanhanen H and Vartiainen E (1995). Reduction of serum cholesterol with sitostanol-ester margarine in a mildly hypercholesterolemic population. N Engl J Med 333: 1308–1312.

Moreau R A, Whitaker B D and Hicks Kevin B (2002). Phytosterols, Phytostanols, and their conjugates in foods: structural diversity, quantitative analysis, and health-promoting uses. Progress in Lipid Res 41: 457-500.

Neil H A, Meijer G W and Roe L S (2001). Randomised controlled trial of use by hypercholesterolaemic patients of a vegetable oil sterol-enriched fat spread. Atherosclerosis 156: 329-337.

Nestel P, Cehun M, Pomeroy S, Abbey M and Weldon G (2001). Cholesterol-lowering effects of plantsterol esters and non- esterified stanols in margarine, butter and low-fat foods. Eur J Clin Nutr 55:1084–1090.

Nguyen T T, Dale L C, Von Bergmann K and Croghan I T (1999). Cholesterol lowering effect of stanols ester in a US population of mildly hypercholesterolemic men and women: A randomized controlled trial. Mayo Clin Proc 74:1198-1206.

Nigon F, Serfaty-Lacrosnière C, Beucler I, Chauvois D, Neveu C, Giral P, Chapman M J and Bruckert E (2001). Plant sterolenriched margarine lowers plasma LDL in hyperlipidemic subjects with low cholesterol intake: Effect of fibrate treatment. Clin Chem Lab Med 39: 634-640.

Normen L, Holmes D and Frohlich J (2005). Plant sterols and their role in combined use with statins for lipid lowering. Curr Opin Investig Drugs 307–316.

Ostlund R E, Racette S B, Okeke A and Stenson W F (2002). Phytosterols that are naturally present in commercial corn oil significantly reduces cholesterol absorption in humans. Am J Clin Nutr 75:1000-1004.

Ostlund R J (2004). Phytosterols and cholesterol metabolism. Curr opin lipidpl 15: 37-41.

Phillips K M, Ruggio D M, Toivo J I, Swank M A and Simpkins A H (2002). Free and esterified sterol composition of edible oils and fats. J Food Compos Anal 15:123–142.

Piironen V and Lampi A M (2003). Occurrence and levels of phytosterols in foods: phytosterols as functional food components and neutraceuticals. Edited by Paresh ,C. Dutta CRC press. Pp:1-32.

Piironen V, Lindsay D G, Miettinen T A, Toivo J and Lampi A M (2000). Plant sterols: biosynthesis, biological function and their importance to human nutrition. J Sci Food and Agric 80: 939-966.

Plat J, Kerckhoffs D and Mensink R (2000). Therapeutic potential of plant sterols and stanols. Curr Opin Lipidol 11: 571–576.

Polagruto J A, Wang-Polagruto J F, Braun M M, Lee L, Kwik-Uribe C, Keen C L (2006). Cocoa flavanol-enriched snack bars containing phytosterols effectively lower total and lowdensity lipoprotein cholesterol levels. J Am Diet Assoc 106:1804-1813.

Pollak O and Kritchevsky D (1981). Sistosterol. Monographs on atherosclerosis. Basel; S. Karger.

Pollak O J (1953). Reduction of blood cholesterol in man. Circulation 7:702-706.

Quilez J P, Garcia-Lorda P and Salas-Salvado J (2003). Potential uses and benefits of phytosterols in diet: present situation and future directions, Clin Nutr 22: 343–351.

Rao A V, Koratkar R (1997). Anticarcinogeniceffects of saponins and phytosterols. ACS Symposium Series 662:313-324.

Reddy K R (2012). Kidwai memorial institute of oncology. Cited on: 2nd Feb’2012. http://kidwai.kar.nic.in/statistics.html

Robinson P L, Cuff T J and Parker J E (2000). Isolation and purification of sterols from neutral fraction of tall oil pitch by single decantation crystallization. US Patent 6 057 462.

Rozner S and Garti N (2006). The activity and absorption relationship of cholesterol and phytosterols. Colloid Surf A Physiochem Eng Asp 282:435-456.

Salen G, Shore V, Tint G S, Forte T, Shefer S, Horak I, Dayal B, Nguyen L, Batta A K, Lindgren F T and Kwiterovich PO Jr (1989). Increased sitosterol absorption, decreased removal, and expanded body pools compensate for reduced cholesterol synthesis in sitosterolemia with xanthomatosis. J Lipid Res 30:131 9-1 330.

SCF (2000). Opinion of scientific committee on Food on A request for the safety assessment of the use of phytosterol esters in yellow fat spreads, 6th April 2000. SCF/CS/NF/DOS/1 FINAL. Eur. Commission, Health and Consumer Protection Directorate General, Brussels

Schonheimer R (1931). New contributions in sterol metabolism. Science 74: 579-584.

Soupas L, Huikko L, Lampi A M and Piironen V (2005). Esterification affects phytosterol oxidation. Eur J Lipid Sci Technol 107: 107-118.

St-Onge M P, Benoit L, Mauger J F, Jones Peter J H (2003). Consumption of a functional oil rich in phytosterols and medium-chain triglyceride oil improves plasma lipid profiles in men. J. Nutr. 133:1815–1820.

Sumner C E, Barnicki S D and Dolfi M D (1995). Process for the production of sterol and Tocopherol concentrates. US Patent 5 424 457.

Vanstone C A, Raeini-Sarjaz M, Parsons W E and Jones P J (2002). Unesterified Plant Sterols and stanols lower LDL- Cholesterol Concentrations Equivalently in Hypercholesterolemic Persons. Am J Clin Nutr 76:1272-1278.

Verleyen T, Forcades M, Verhe R, Dewettinck K, Huyghebaert A and De Greyt W (2002) Analysis of free and esterified sterols in vegetable oils. J Am Oil Chem Soc 79:117-122.

Volpe R, Niittyen L, Carpel R, Sitori C, Bucc I A, Fraone N and Pazzucconi F (2001). Effects of yoghurt enriched with plant sterols on serum lipids I patients with moderate hypercholesterolemia. Br J Nut, 86:233-239.

Waalkens-Berendsen H D, Wolterbeek A P M and Wijnands M V W (1999). Safety evaluation of phytosterol sters. Part 3. Two generation reproduction study in rats with phytosterol esters- a novel functional food. Food Chem Toxicol 37:683-696.

Wasowicz E (2002). Cholesterol and phytosterols. In: chemical and functional properties of food lipids. Zdzislaw E. Sikorski and Anna Kolakowska (Editors). CRC Press.

Weihrauch J L and Gardner J M (1978). Sterol content of foods of plant origin. J Am Dietet Assoc 73: 39-47.

Weststrate J and Meijer G (1998). Plant sterol-enriched margarines and reduction of plasma total- and LDL-cholesterol concentrations in normocholesterolaemic and mildly hypercholesterolaemic subjects. Eur J Clin Nutr 52: 334-343.

Wolfs M, De Jong N, Ockéa M C, Verhagen H and Verschuren Monique W M (2006). Effectiveness of customary use of phytosterol/-stanol enriched margarines on blood cholesterol lowering. Food Chem Toxicol 44:1682-1688.

Worthington R E and Hitchcock H L (1984). A method for the separation of seed oil steryl esters and free sterols: application to peanut and corn oils. J Am Oil Chem Soc 61:1085-1088.

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