Utility of rapeseed meal (RSM) as a rich source of protein is limited due to presence of glucosinolates and phytates. The efficacy of moist heat, fermentation, germination and preparation of isolates with/without these treatments, in reducing these antinutritional factors (AFs) has been investigated. Single step alkali treatment of RSM, both before and after germination, resulted in 97% extraction of protein, However, fermented and moist heated RSM yield 89.3 and 71.9% protein. Most of the proteins were recovered by sequential isoelectric precipitation (pH 6.0 and 3.6) of alkali extracts of treated/untreated RSMs. Contents of minerals (Mn, Fe, Cu & Zn), oil, soluble sugars, lipids and crude protein remained unaffected in all the treatments. Tryptophan was unaffected but decreased in isolates from treated RSMs. Methionine was also unaffected upon mOist heating and fermentation, and isolate I prepared from fermented RSM. Moist heating and fermentation decreased cysteine in RSM as well as its isolates. All the treatments reduced phytate by about 50% in RSM as well as isolates, but germination was ineffective in reducing phytate in isolates. Glueosinolatc content of RSM was found to be lowered by these treatments. Rapeseed meal with reduced glueosinolate & phytate could find useful application in supplementing animal feeds.