Asian Journal of Dairy and Food Research, volume 26 issue 3 & 4 (2007) : 164-168

COMPARATIVE STATUS OF BLOOD METABOLITES WITH COMMENCEMENT OF CYCLICITY IN POST-PARTUM LACTATING RIVERINE BUFFALOES (BUBALUS BUBALIS)

A. Mishra*, P.K. Pankaj, B.S. Prakash
1National Dairy Research Institute, Karnal-132001, Haryana (India)
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Cite article:- Mishra* A., Pankaj P.K., Prakash B.S. (2024). COMPARATIVE STATUS OF BLOOD METABOLITES WITH COMMENCEMENT OF CYCLICITY IN POST-PARTUM LACTATING RIVERINE BUFFALOES (BUBALUS BUBALIS). Asian Journal of Dairy and Food Research. 26(3): 164-168. doi: .
Twenty-two post-partum riverine buffaloes (Murrah breed) were selected from National Dairy Research Institute (NDRI) Karnal herd of first or second lactation to determine the length of post-partum period for cyclicity commencement by blood metabolites viz., non-esterified fatty acid (NEFA), glucose and alpha amino nitrogen (AAN). Blood samples (5-10 ml) were collected twice a week until commencement of cyclicity as determined by progesterone analysis or for period of 3 months (whichever is earlier). Out of these twenty-two animals, ten were found out to be cyclic as determined through progesterone analysis. Blood samples were collected twice a week at 3-4 days interval from all animals by means of jugular vein puncture 5 days post-partum period. To fulfill the objectives of the present study, plasma NEFA, Glucose and AAN were assayed by Copper soap solvent extraction method modified by Shipe, GOD/POD method and Goodwin method respectively. Plasma progesterone was analyzed by a Direct Radioimmunoassay (RIA) method. Concentrations of blood metabolites viz. glucose and NEFA are unaffected by days for commencement of cyclicity and were also were non-significant (P>0.05).
    1. Beam, S.W. and Butler W. R. (1997). Biol. Reprod. 56: 133-142.
    2. Domecq, J. J., et al. (1997). J. Dairy Sci., 80: 113-120.
    3. Goodwin J.F. (1970). Stand. Meth.Clin.Chem.6:89-98.
    4. Grimard, B.P., et al (1995). J. Reprod. Fertil. 104: 173-179.
    5. Hart, I.C., et al. (1978). J. Endocrinol. 77:333.
    6. Hennies, M and Holtz , W.(1993). J. Immunol Methods. 157:149-157.
    7. Herbein J, H., et al. (1985) J. Dairy Sci. 68:320-325.
    8. Johari, M.P. (1960). Indian Vet. J. 37:354-364.
    9. Kamboj, M. and Prakash, B.S. (1993). Tropical Anim. Hlth. and Prod. 25:185-192.
    10. Koops, J. and Klomp, H.K. (1977). Netherlands Milk Dairy J., 31: 56-58.
    11. Prakash, B. S. and Madan, M. L. (2001). Indian. J. Anim. Sci. 71(3):251-253.
    12. Shipe, W.F., et al. (1980). J. Dairy Sci., 63: 193-198.
    13. Snedecor, G.W. and Cochran, W.G (1968). Statistical Methods. Oxford & IBH Pub. Co.., New Delhi.

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