Asian Journal of Dairy and Food Research, volume 32 issue 2 (june 2013) : 91-95

CHARACTERIZATION OF CASEINOPHOSPHOPEPTIDES (CPPS) PRODUCED FROM A AND B-CASEIN FRACTIONS USING YOGHURT CULTURES

D. Kusuma, Jayashri P. Hiremath*, G.S. Bhat
1Department of Dairy Chemistry, Dairy Science College, Bangalore- 560 024, India
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Cite article:- Kusuma D., Hiremath* P. Jayashri, Bhat G.S. (2024). CHARACTERIZATION OF CASEINOPHOSPHOPEPTIDES (CPPS) PRODUCED FROM A AND B-CASEIN FRACTIONS USING YOGHURT CULTURES. Asian Journal of Dairy and Food Research. 32(2): 91-95. doi: .
The a-sand b-casein fractions were fractionated from whole casein obtained from cow milk. 10% dispersions of aand bcasein were  fermented using purified 1% Streptococcus thermophilus (ST) & Lactobacillus bulgaricus (LB) cultures separately. Conditions were optimized by varying lactose concentration. (2,3 &4%) and incubation time (12-48h). Caseinophosphopeptides were isolated by adjusting pH at 4.6, in presence of ethanol and 1% CaCl2. CPPs from acasein fermented with LB showed higher yield (8% at 12h), mineral binding (0.45mg/mg of CPPs) and solubilizing ability (12.2mg/mg of CPPs) compared to other types indicating more hydrophilic peptides which was confirmed by RP-HPLC analysis. SDS-PAGE of the CPPs revealed molecular weight ranging between 3000-6500Da. The study concluded that LB has higher proteolytic activity than ST and may be used in the cost effective production of CPPs.
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