<div>Background: Induced mutagenesis was employed in horse gram cv. CRIDA 1-18R with an objective of evolving mutants with increased yield potential.&nbsp; 
 
Methods: Based on preliminary study, the variety has been irradiated with desirable doses of gamma rays (200 Gy and 300 Gy), electron beam (100 Gy and 200 Gy) and its combination (100 Gy) and the material were forwarded to M2 generation following parent-progeny row basis. The genotypes exhibiting superior yield than control were further forwarded to M3 generation. Multivariate analyses were performed to determine the induction of micro-mutants for 11 quantitative traits. 
 
Result: The reduction in mean value over control was observed for most of the yield component traits at M2 generation. Among the mutagenic treatments, combination of gamma rays with electron beam registered high mean values for pod length (5.05 cm), number of pods per plant (54.42) and single plant yield (25.68 g) at M3 generation. Non-significant skewness and / or kurtosis at M3 population denoted the absence of epistatic interactions for plant yield. An increase in variability pattern, H2 and GAM at M3 generation indicated the scope for trait (plant height, number of seeds per pod, number of clusters per plant, number of pods per plant, biological yield and single plant yield) improvement through selection.</div>

<div>Generation of Micro-mutants for Yield and Component Traits in Horse Gram [Macrotyloma uniflorum (Lam.) Verdc.]</div>

Background: The pulse known as &ldquo;Blackgram&rdquo; is a significant component of the Indian diet since it provides vegetable protein and balances the diet centered on cereals. Mungbean Yellow Mosaic Virus (MYMV) outbreaks on a regular basis completely destroy crops, causing farmers to suffer enormous losses. Mutation breeding can be an effective strategy for developing crops with desirable traits, such as high yield and resistance to diseases like the Mungbean Yellow Mosaic Virus (MYMV) in blackgram. 
&nbsp; 
Methods: This experiment was laid out to study the variability pattern of mutant population in M4&nbsp;and M5&nbsp;generation of blackgram variety CO 6 treated with the Gamma rays at 200 Gy, 300 Gy and 400Gy and isolation of viable mutants for Mungbean Yellow Mosaic virus resistance. Significant variability was observed among the different treatments in M4 generation and twenty MYMV resistant mutants ((PKT-BG-200Gy-P07; PKT-BG-200Gy-P24; PKT-BG-200Gy-P29; PKT-BG-200Gy-P35; PKT-BG-200Gy-P36; PKT-BG-200Gy-P45; PKT-BG-300Gy-P13; PKT-BG-300Gy-P16; PKT-BG-300Gy-P28;&nbsp; PKT-BG-300Gy-P33; PKT-BG-300Gy-P41; PKT-BG-300Gy-P4; PKT-BG-300Gy-P43; PKT-BG-300Gy-P76; PKT-BG-300Gy-P81; PKT-BG-300Gy-P88; PKT-BG-300Gy-P93; PKT-BG-400Gy-P15; PKT-BG-400Gy-P21; PKT-BG-400Gy-P23)&nbsp; were selected and evaluated in a randomized block design with three replications under natural conditions.&nbsp; Scoring was done by the modified scale of All India Coordinated Research Project on MuLLaRP. 
&nbsp; 
Result: Incidence ranged from scale 0.01 (PKT-BG-300Gy-P41and PKT-BG-300Gy-P42) to 5.30 (PKT-BG-300Gy-P28). The mutants PKT-BG-300Gy-P41 and PKT-BG-300Gy-P42 recorded with maximum yield of 1500 kg/ha and 1445kg/ha were found to be observed as high yielding and MYMV resistant lines for summer season&nbsp; in the Cauvery delta region.

Evaluation and Isolation of Mungbean Yellow Mosai Virus Resistant Mutants in M4 and M5 Mutant Generations of Blackgram (Vigna mungo)

This study was conducted to evaluate the concentration of micro minerals (Zn, Fe, Mn, Co and Cu) of feeds and their distribution in fiber fractions (Neutral Detergent Fiber/NDF and Acid Detergent Fiber/ADF). Three species of common feeds namely ground nut cake (Arachis hypogaea), mustard cake (Brassica juncea), soybean meal (Glycine max), were collected. Micro minerals concentration in the feed samples and fiber fractions were analyzed using Inductively Coupled Argon Plasma Optical Emission Spectrophotometer (ICP-OES) Icap 6000 Series. The results showed that micro minerals concentration of feed and their distribution in fiber fraction varied among species. The concentration of all the minerals in feed was above the critical level. Distribution of micro minerals in NDF and ADF were also estimated. Iron (Fe), copper (Cu), zinc(Zn), in feeds were the highest elements associated in NDF and ADF, while the lowest was found in cobalt (Co) and manganese(Mn).

Micro mineral distribution on fiber fraction of feeds

<div>Background: Induced mutagenesis has been used in citrus breeding, in Georgia, since 1960, resulting in many interesting and promising mutant forms that require morphological, physiological and biochemical studies. The present paper presents the results of biochemical research of some promising mutants.&nbsp; 
 
Methods: During the period 2017-2019 investigate on 13 promising mutant forms of mandarin has been carried out. In the current study, phenolic composition content in the fruits of mandarin mutants were determined using Ultraviolet Spectrophotometer and High Performance Liquid Chromatography (HPLC) and their antioxidant capacities were evaluated using 2.2-diphenyl-1-picrylhydrazyl (DPPH) radicals. 
 
Result: The results have shown that different concentrations of mutagen effect on mandarin seeds and, accordingly, on the biochemical components which they include. In most cases, this leads to a positive result. The impact of the mutagen significantly increases the range of changes in biochemical components and varies over a wide range. Analyzing the data, we can conclude that the solutions of all concentrations of the Nash mutagen effect a change in the biochemical indicators in mutant forms. However, a wide range of variability is largely associated with the use of medium and low concentrations of the mutagen. In most cases, the majority of mutant forms significantly outperform the control variants in the content of biochemical components. The study has revealed a variety of mutant forms (gene pool), which characterized a whole complex of positive signs. Their use in selection allows getting perspective forms with positive features.</div>

<div>Results of the Biochemical Study of Mandarin (Citrus reticulata Blanco) Mutants</div>

<div>Background: Soybean is an important source of food, protein and oil and hence more research is essential to increase its yield under different agro-ecological conditions, including stress. In this regard, four popular soybean varieties viz. Shohag, BDS-4, BAU-S/64 and BARI Soybean-5 were irradiated using Co60 gamma rays to create genetic variation for earliness, higher seed yield and other desirable agronomic traits. 
 
Methods: The experiments were conducted at Bangladesh Institute of Nuclear Agriculture (BINA) Headquarters farm, Mymensingh during 2006-2009 and 28 elite mutant lines were selected for evaluation. The mutant line, SBM-22 derived from mother variety BARI Soybean-5 irradiated with 300Gy of gamma rays was found to be superior compared to other mutants. Considering the superior performance of mutant SBM-22 including 28 mutants and mother check variety BARI Soybean-5, were evaluated through different trials. The evaluation trials were conducted at different agro-ecological zones of the country during Rabi season (January to April) of 2010-2018. 
 
Result: Significant variations were observed both in individual location and over locations for all traits. Reactions to major diseases and insect-pests infestation were also studied. Due to better performance of the mutant SBM-22, Bangladesh Institute of Nuclear Agriculture (BINA) applied to the National Seed Board (NSB) of Bangladesh for registration as an important soybean variety &ldquo;Binasoybean-6&rdquo;. Consequently, the NSB of Bangladesh registered SBM-22 as an improved soybean variety in 2019 as Binasoybean-6 for commercial cultivation.</div>

​Binasoybean-6: A High Yielding Mutant Soybean Variety Developed through Sustainable Mutation Breeding

Micro-irrigation technologies have been an integral part of modern agriculture. Ranging from &lsquo;increased crop yield&rsquo; to &lsquo;improved economic benefit,&rsquo; such systems have much to offer. However, costly installation procedures and high socioeconomic barriers restrict their diffusion with relatively cheap alternatives. Appropriate policies and innovative approaches enable crossing these barriers only if carefully crafted to meet economic, technological and socio-economic challenges. This review offers an overview of significant strategic policies and new-generation micro-irrigation technologies that would help tap their full potential. Based on previous studies and individual experiences from India, an assessment of the transformative impacts of micro-irrigation has been done. These micro-irrigation technologies are proving highly beneficial for Maharashtra. The main gains that accrued to farmers from the process include reduced costs of cultivation, lesser dependence on chemicals and pesticides, conservation of water resourcesand less expenditure on labor. The above results manifest the contribution or role of micro-irrigation to accelerate sustainable agriculture, which induces a second green revolution imbued with higher productivity, efficiency in resource mobilization and economic prosperity.

Impacts of Micro-irrigation Technologies in Maharashtra: A Review

<div style="text-align:justify">Background: Semi-arid regions have a large livestock population that grazes on nutrient-depleted natural grasses and crop leftovers, resulting in mineral shortages. The goal of this study was to determine the amounts of iron (Fe), copper (Cu), manganese (Mn) and zinc (Zn) in soil, pasture and sheep serum in semi-arid areas with low rainfall (498-522 mm) in order to construct a soil-plant-animal continuum and recommend solutions to increase trace element availability. 
 
Methods: Samples were collected from different parts of the continuum [Soil (n=50), pasture (n=50) and blood samples from sheep (n=50)] from the selected study area and the trace elements were analyzed after digestion using atomic absorption spectrophotometer. Descriptive, correlation and t-test analyses were carried out to categorize the deficiency, their significance and soil-plant-animal continuum.&nbsp; 
 
Result: Although most soil samples were found to be below threshold levels, most straws and stovers in the field had marginal levels of these micro-minerals. Most sheep serum samples had levels of different micro-minerals that were below the threshold level, implying that many indigenous sheep are deficient in micro-minerals. Although there was a substantial association between feed, fodder and sheep serum, there was no such correlation between trace mineral levels in sheep serum and soil. A higher percentage of these micro-minerals in sheep faeces implies that these nutrients are truly recycled through faeces and urine in semi-arid rural areas. To address the sub-clinical micro-mineral insufficiency in semi-arid regions, micro-mineral supplementation, particularly Zn, Cu and Mn, is required.</div>

Soil-plant-animal Continuum Concerning the Certain Micro-mineral Status of Indigenous Sheep in Hot Semi-arid Regions

Background:&nbsp;Soybean is one of the most important commodities around the globe. Increasing&nbsp; soybean production absolutely continue to be pursued because soybean consumption increased steadily. One way that can be done is assembly technology of new superior varieties that have better and adaptive properties. The aim of this study was to determine molecular diversity of soybean mutants lines resulting from gamma irradiation in M6 generation.&nbsp; 
 
Methods: Molecular analysis with RAPD markers was carried out in the Tropical Balitbu Laboratory Sumatera Barat, Indonesia in November 2021. The tested genotypes consisted of 8 genotypes, namely Kipas Putih variety (elderly), Anjasmoro variety (comparison), M.1.1.3, M.5.2.1, M.5.2.3 l, M.1.1.8, M.1.1.9 and M.1.1.17 mutant lines. The primers used were RAPD 2 (5&acute;-GTTTCGCTCC-3&acute;), RAPD. 3 (5&acute;-GTAGACCCGT-3&acute;), RAPD 4 (5&acute;-AAGAGCCCGT-3&acute;) and RAPD 5 (5&acute;-AACGCGCAAC-3&acute;).&nbsp; 
 
Result: The results of the RAPD analysis showed that there were differences in size of amplicon between the mutants and the parents, namely the RAPD 2 primer (1400 bp band) and the RAPD 5 primer (550 bp and 1000 bp band). The parents had a pattern of DNA bands but in the M.5.2.3 and M.1.1.9 mutants the DNA band was absent. The parent RAPD 5 primers (1000 bp, 550 bp) had a DNA banding pattern but in the M.1.1.3 and M.1.1.8 mutants there was no DNA banding pattern. This indicated that the M.1.1.3, M.5.2.3, M.1.1.8 and M.1.1.9 mutants had different genetic diversity from their parent (Kipas Putih variety).

RAPD Analysis for Genetic Variability of Soybean Mutant Resulting from Gamma Rays Irradiation in M6 Generation

Background: Horse gram is the potential rainfed legume in Indian farming. The major limitation in horse gram breeding is the narrow variability. Variability induction and its estimation would sustain food security.&nbsp; 
 
Methods: Variability was induced using gamma rays, electron beam and ethyl methanesulfonate and their combinations. The induced variation for the economic traits, their inheritance and genetic gain were ascertained.&nbsp; 
 
Result: The analysis of variance indicated the induction of significant variation for yield attributing traits. The population was grouped into 10 constellations by the virtue of induced variation. The groups I, II and V were the largest comprising of 38, 31 and 19 mutants respectively. The mutants exhibited significant intra and inter group variation. The mutagens induced the maximum variability for plant height (32.24%), 100 seed weight (25.42%) and number of pods per plant (19.18%). The mutants possessed high genotypic and phenotypic coefficients of variation for all the characters except flowering traits and duration. The induced variability for the yield attributing traits possessed significant breeding value as the heritability (86.66%-99.72%) and genetic advance as percent of mean (10.65-81.94) were high and the environmental influence was the minimum.

Diversity Analysis in Horse Gram [Macrotyloma uniflorum (Lam.) Verdc.] Mutants

<div>Background: A systematic investigation of the induced mutagenesis in Urdbean was attempted with cultivars viz., MDU 1 and VBN (Bg) 4 to generate superior battering quality varieties. Induced mutation is proven as an applicable breeding method to build variation in plant materials. In this study, 40 Urdbean genotypes including thirty eight mutants of two promising Urdbean varieties namely MDU1 and VBN (Bg) 4 and two parental genotypes were involved for quality analysis. One of the most dangerous diseases in legumes is Mungbean Yellow Mosaic Virus (MYMV). In legume, this virus causes enormous losses in the areas of Urdbean cultivation. The prevailing varieties are partially resistant to this virus. Hence, the paramount approach to control this disease is breeding for resistance or tolerance to this disease. There is a necessity of searching the sources of disease resistance. In this study, screening of the mutants was conducted to make out the source of resistance to this disease. 
 
Methods: The nutritional characters viz., albumin, globulin and total soluble protein along with biochemical analysis of battering quality through Arabinose estimation were analyzed to find out the quality characters of the mutants. Batter volume analysis from idli batter was conducted conducted. Scoring of disease severity was done for all the plants on 50th day on the basis of visual scoring. The disease was scored on a 1-9 arbitrary scale. For biochemical characterization for this disease estimation of total phenol content, total sugars and phytic acid was executed. 
 
Result: Based on the general performance of albumin content, globulin content, total soluble protein, arabinose content, 100 seed weight and seed yield per plant, the mutant lines viz., ACM-014-021, ACM-015-015, ACM-15-023, ACM-015-013, ACM-015-003, ACM-015-030, ACM-014-006, ACM-014-007 were recognized as the best. Hence these mutant lines could be exploited directly in the breeding programme to develop superior battering quality Urdbean varieties. Based on over all mean performance of important batter volume characters viz., initial batter volume analysis, final batter volume, improved batter volume, arabinose content and single plant yield the mutant lines ACM-15-015, ACM-015-030, ACM-015-003, ACM-015-023 were identified to be superior in terms of yield, arabinose content and batter volume. The mutant lines viz., ACM-014-021, ACM-015-025, ACM-014-006, ACM-015-022, ACM -015-023, ACM-014-019, ACM-014-007, ACM-015-017, ACM-014- 003 and VBN (Bg) 4 were recognized as resistant to MYMV. These lines had higher total phenol, phytic acid content and less total sugar content in the leaves when compared with the highly susceptible mutant lines. The present study indicated that phytic acid content recorded highly significant positive correlation with total phenol. &nbsp;Based on the per se performance for all the characters studied viz., seed batter analysis, seed volume analysis and MYMV scoring, the mutant lines viz., ACM-014-021, ACM-015-23, ACM-015-030, were judged as the best and could be exploited directly or in breeding experiments for improving yield along with batter quality. &nbsp;</div>

<div>Genetic and Biochemical Characterization of Electron Beam and Gamma Ray Induced Mutants for Superior Battering Quality Trait and MYMV in Urdbean [Vigna mungo (L.)]</div>

Generation of Micro-mutants for Yield and Component Traits in Horse Gram [Macrotyloma uniflorum (Lam.) Verdc.]

Evaluation and Isolation of Mungbean Yellow Mosai Virus Resistant Mutants in M₄and M₅ Mutant Generations of Blackgram (Vigna mungo)

Micro mineral distribution on fiber fraction of feeds

Results of the Biochemical Study of Mandarin (Citrus reticulata Blanco) Mutants

Binasoybean-6: A High Yielding Mutant Soybean Variety Developed through Sustainable Mutation Breeding

Impacts of Micro-irrigation Technologies in Maharashtra: A Review

Soil-plant-animal Continuum Concerning the Certain Micro-mineral Status of Indigenous Sheep in Hot Semi-arid Regions

RAPD Analysis for Genetic Variability of Soybean Mutant Resulting from Gamma Rays Irradiation in M₆ Generation

Diversity Analysis in Horse Gram [Macrotyloma uniflorum (Lam.) Verdc.] Mutants

Genetic and Biochemical Characterization of Electron Beam and Gamma Ray Induced Mutants for Superior Battering Quality Trait and MYMV in Urdbean [Vigna mungo (L.)]