<div>Background: The surgical reconstruction of large abdominal defects with application of surgical mesh of synthetic origin resulted a number of potential clinical complications; although it is having good tensile strength. Acellular bio-mesh derived from slaughtered animals could be better alternative for surgical management abdominal wall defects. The present study aimed to evaluate bio-detergent based decellularized allogenic porcine acellular diaphragm matrix in umbilical hernioplasty in pig. 
 
Methods: For the present study sodium deoxycholate was used as bio-detergent for preparation of porcine allogenic acellular diaphragm matrix (ADM). All total eighteen (18) numbers of piglets aged 2-3 months, body weight 8-14 kg and either sex with umbilical hernia equally divided in to three groups. Group A was subjected to application of ADM; while in Group B injection of methylprednisolone along with ADM application and Autologous Tunica Vaginalis was used for hernioplasty in Group C. Various parameters viz clinical, gross evaluation of surgical wound, haemato-biochemical and indirect ELISA of blood and serum was carried out as per standard methods.&nbsp; 
 
Result: Decellularization protocol carried out with sodium deoxycholate as bio-detergent@ 2% concentration resulted complete decellularization of porcine diaphragm and preserved the distinctive, natural, three-dimensional collagen structure within the prepared matrix. Clinical and haemato-biochemical observations following umbilical hernioplasty in pig with ADM promises its clinical utility. The immune response of host tissue to bio-mesh as revealed by Indirect ELISA was negligible.</div>

Preparation of Porcine Allogenic Acellular Diaphragm Matrix and its Application for Umbilical Hernioplasty in Pig

<div>Background: Tissue-engineered scaffolds for skin wound healing have undergone marvelous progress. The recognition that a three-dimensional scaffold more closely mimics the biomechanical environment of wounds and advancing knowledge of cell biology has led to the next-generation of engineered bioscaffolds with nanotechnology. A unifying approach is required for the translational success of bioscaffolds, involving clinicians, biologists and chemists. The decellularized materials were expanding their clinical utility due to high clinical results ahead of the ones with autografts. They are gradually gaining market space due to their ease of standardized production, constant availability for grafting and biomechanical/ biochemical advantage. Hence, the present study aimed to develop biobased decellularized extracellular matrix (dECM) impregnated with eco-friendly synthesized silver nanoparticles and then to evaluate their wound healing activity the excision wound model of rats.&nbsp; 
 
Methods: The dECM was prepared to achieve acellularity, intactness and adequate tensile strength. The same was confirmed by morphological (histology, scanning electron microscopy and Fourier transform infrared spectroscopy), mechanical (tensile strength) and biological (DNA quantification) analyses. Further, the dECM was impregnated with the eco-friendly synthesized keratin-chitosan-silver nanoparticles to produce &lsquo;NanoBioscaffolds&rsquo; for extended biocompatibility and were evaluated for wound healing activity in rats. &nbsp; 
 
Result: The findings of the histopathology (H and E staining), immunohistochemistry (the proliferative activity of keratinocytes by Ki67 staining) and biochemical analysis (anti oxidative status by catalase estimation) revealed that wound healing activity was promoted by inducing proliferation and migration of the keratinocytes and detoxification of reactive oxygen species activity (ROS) in NanoBioscaffold treated group.</div>

<div>NanoBioscaffolds as Wound Healing Biomaterials in Animals</div>

Background: In dogs the corneal ulcers are one of the major ophthalmic emergencies and can cure with routine medications, but in delay presentation will become challenge for veterinarians. In such cases advanced therapeutic management such as serum, platelet reach plasma and corneal grafts are needed for favorable outcome.&nbsp;&nbsp; 
 
Methods: The present study was conducted on 24 dogs (29 eyes) with corneal ulcer categorized as a superficial (Group-I, n=15), deep corneal ulcers (Group-II, n=8) and descemetocele (Group-III, n=6) which were treated with autologous serum, Platelet Rich Plasma and Processed decellularized human amniotic membrane grafting, respectively. All the affected eyes were given routine topical medications with eye drops Moxifloxacin, Flurbiprofen q.i.d till healing, 1% Atropine sulphate b.i.d for 5 days and Oral Doxycycline was given @ 5 mg/kg b.i.d for 10 days. 
 
Result:&nbsp;The group-I (n=11, 15 eyes) treated with autologous serum eight to ten times-a-day as per severity with routine medication. Two dogs with entropion which was corrected first. The eyes gained vision within 10th-15th days. Developed complications managed medicinally till complete recovery. The group-II (n=7, 8 eyes) treated with platelet rich plasma drops five to seven times-a-day with routine medication. 75% eyes recovered up to 45th day. Developed complication were managed medicinally till complete recovery. The group-III (n=5, 6 eyes) treated with Decellularized Processed Human Amniotic Membrane Graft. 4 eyes gained complete transparency on 28th day of therapy while in 2 eyes developed complication were managed medicinally.

Preparation of Porcine Allogenic Acellular Diaphragm Matrix and its Application for Umbilical Hernioplasty in Pig

NanoBioscaffolds as Wound Healing Biomaterials in Animals

Management of Corneal Ulcers using Autologous Serum, Platelet Rich Plasma and Processed Human Amniotic Membrane in Dogs