Response of Microorganisms in Alfalfa Rhizosphere to Microbial Inoculation

DOI: 10.18805/LR-514    | Article Id: LR-514 | Page : 706-710
Citation :- Response of Microorganisms in Alfalfa Rhizosphere to Microbial Inoculation.Legume Research-An International Journal.2020.(43):706-710
Snežana Andjelković, Jasmina Radović, Snežana Babić, Tanja Vasić, Simonida Djurić, Dragana Stamenov, Timea Hajnal Jafari snezana.andjelkovic@ikbks.com
Address : Institute for forage crops Kruševac, 37251 Globoder, Republic of Serbia.
Submitted Date : 3-07-2019
Accepted Date : 2-09-2020

Abstract

Background: Various microorganisms that can have a positive or negative effect on plant development are present in the rhizospheric soil of alfalfa. The research aimed to investigate the impact of two nitrogen-fixing bacteria and two species of the phytopathogen fungus Colletotrichum on the abundance of aminoheterotrophs, nitrifying and denitrifying bacteria in the rhizosphere of three cultivars of alfalfa.
Methods: The experiment was carried in vegetation pots as three factorial, where the first factor was alfalfa cultivar (Affinity+Z, K-28 and Perry), the second was the isolate of phytopathogen fungus Colletotrichum: C. trifolii (isolate Coll 4) and C. destructivum (two isolates: Coll-11 and Coll 657); and the third was the variant of bacterial inoculation (Azotobacter chroococcum and Sinorhizobium meliloti). The number of microorganisms was determined by introducing a diluted soil suspension into proper media and counted per one gram of absolutely dry soil.
Result: According to the Fisher test applied inoculation microbial inoculation with nitrogen-fixing bacteria and phytopathogen fungus had different effects on the abundance of examined microorganisms in rizospheric soil of different alfalfa cultivars. 

Keywords

Alfalfa Cultivar Isolate Microbial Inoculation

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