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In vitro plant regeneration in pigeonpea [Cajanus cajan (L.) MILL Sp.] using various explants
Laboratory of Plant Tissue Culture, Department of Biotechnology, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India.
abhijeetanandha@ymail.com
Abstract
An efficient method for plant regeneration via callus formation has been developed in pigeonpea using two varieties as BDN-2 and GT-101. Three different explants viz. leaf, hypocotyl and root excised from 15-20 days old in vitro raised seedlings were cultured on basal MS medium. Greenish coloured callus were observed using various media according to explants. More shoots were found in MS + 1.0 mg/l NAA + 4.0 mg/l Adenine and roots on Elongated shoots in MS + 1.0 mg/l IAA media. Rooted plants were transferred in pot for acclimatization.
Keywords
Callus, In vitro, Pigeonpea, Regeneration
References
- Aboshama, H.M.S. (2011). Somatic embryogenesis proliferation, maturation and germination in Cajanus cajan. World J. Agril. Sci., 7(1): 86-95.
- Anbazhagan, V.R. and Ganapathi, A. (1999). Somatic embryogenesis in cell suspension cultures of pigeonpea (Cajanus cajan). Pl. Cell Tissue Organ Cult., 56: 179-184.
- Anonymous, (2011). Area and productivity of pigeonpea in India. (http://www.ncipm.org.in).
- Anonymous, (2012). Area, production and productivity of pigeonpea in Gujarat. (www.autherstream.com).
- Geetha, N., Venkatachalam, P., Prakash, V. and LakshmiSita, G. (1998). High frequency induction of multiple shoots and plant regeneration from seedling explants of pigeonpea (Cajanus cajan L.). Curr. Sci., 75: 1036-1041.
- George, L. and Eapen, S. (1994). Organogenesis and embryogenesis from diverse explants of pigeonpea. Pl. Cell Rep., 13: 417-420.
- Jain, M. and Chaturvedi, H.C. (2004). In vitro cloning of Cajanus cajan var. Bahar through prolific shoot bud differentiation in leaf segments and production of fertile plants. Indian J. Biotech., 3: 258-262.
- Joshi, P.K., Rao, P.P., Gowda, C.L.L., Jones, R.B., Silim, S.N., Saxena, K.B. and Kumar, J. (2001). The world chickpea and pigeonpea economies: facts, trends, and outlook. Patancheru 502 324, Andhra Pradesh, India: International Crops Research Institute for the Semi-Arid Tropics.
- Krishna, G., Reddy, P.S., Ramteke, P.W., Rambabu, P., Sohrab, S.S., Rana, D. and Bhattacharya, P. (2011). In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105. Physiol. Mol. Biol. Pl., 17(4): 375-385.
- Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Pl., 15: 473–497.
- Mohan, M.L. and Krishnamurthy, K.V.(1998). Plant regeneration in pigeonpea [Cajanus cajan (L.) Millsp.] by organogenesis. Pl. Cell Rep., 17: 705-710.
- Prabhakaran, M., Elumalai, S., Ramganesh, S. and Prakasam, V. (2011). High frequency induction of callus from seedling explants of pigeonpea (Cajanus cajan [L] Millsp.) for genetic transformation. Curr. Bot., 2(6):15-17.
- Prabhakaran, M. and Elumalai, S. (2014). An efficient in vitro regeneration of shoot from cotyledon derived callus of pigeonpea [Cajanus cajan (L.) Millsp.]. Indian Streams Res. J., 3(12): 1-7.
- Prasad, M.G., Prasad, T.N.V.K.V. and Sundhakar, P. (2011). In vitro proliferation of shoot regeneration from embryo of Cajanus cajan L. (var. LGG-29). J. Develop. Biol. Tissue Engg., 3(5): 62-65.
- Sreenivasu, K., Malik, S.K., Ananda, Kumar, P. and Sharma, R.P. (1998). Plant regeneration via somatic embryogenesis in pigeonpea (Cajanus cajan L. Millsp). Pl. Cell Rep., 17: 294-297.
- Steel, R.G.D. and Torrie, J.H. (1960). Principle and Procedures of Statistics. McGraw Hill Book Company, Inc. New York, pp. 99-128.
- Varshney, R.K., Penmetsa, R.V., Dutta, S., Kulwal, P.L., Saxena, R.K., Datta, S., Sharma, T.R., Rosen, B., Carrasquilla-Garcia, N., Farmer, A.D., Dubey, A., Saxena, K.B., Gao, J., Fakrudin, B., Singh, M.N., Singh, B.P., Wanjari, K.B., Yuan, M., Srivastava, R.K., Kilian, A., Upadhyaya, H.D., Mallikarjuna, N., Town, C.D., Bruening, G.E., He, G., May, G.D., McCombie, R., Jackson, S.A., Singh, N.K. and Cook, D.R. (2010). Pigeonpea genomics initiative (PGI): an international effort to improve crop productivity of pigeonpea (Cajanus cajan L.). Mol. Breed., 26(3): 393–408.
- Venkatachalam, P. and Lakshmi Sita, G. (2008). Genetic transformation as a tool for improvement of Pigeon Pea, (Cajanus cajan [L.] Millsp.) Handbook of New Technologies for Genetic Improvement of Legumes, p: 125–145.
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