Legume Research

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Research Article

Legume Research, volume 41 issue 2 (april 2018) : 226-229

In vitro plant regeneration in pigeonpea  [Cajanus cajan (L.) MILL Sp.] using various explants

K. Nandha Abhijeeta, B. Madariya Rajesh
1<p>Laboratory of Plant Tissue Culture, Department of Biotechnology, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India.</p>
Cite article:- Abhijeeta Nandha K., Rajesh Madariya B. (2017). In vitro plant regeneration in pigeonpea [Cajanus cajan (L.) MILL Sp.] using various explants . Legume Research. 41(2): 226-229. doi: 10.18805/lr.v0i0.7844.

ABSTRACT

An efficient method for plant regeneration via callus formation has been developed in pigeonpea using two varieties as BDN-2 and GT-101. Three different explants viz. leaf, hypocotyl and root excised from 15-20 days old in vitro raised seedlings were cultured on basal MS medium. Greenish coloured callus were observed using various media according to explants. More shoots were found in MS + 1.0 mg/l NAA + 4.0 mg/l Adenine and roots on Elongated shoots in MS + 1.0 mg/l IAA media. Rooted plants were transferred in pot for acclimatization.

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