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In vitro plant regeneration in pigeonpea  [Cajanus cajan (L.) MILL Sp.] using various explants

K. Nandha Abhijeeta and B. Madariya Rajesh 

Laboratory of Plant Tissue Culture, Department of Biotechnology, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India.

abhijeetanandha@ymail.com

Page Range:
226-229
Article ID:
LR-3739
Online Published:
6-05-2017
Abstract

An efficient method for plant regeneration via callus formation has been developed in pigeonpea using two varieties as BDN-2 and GT-101. Three different explants viz. leaf, hypocotyl and root excised from 15-20 days old in vitro raised seedlings were cultured on basal MS medium. Greenish coloured callus were observed using various media according to explants. More shoots were found in MS + 1.0 mg/l NAA + 4.0 mg/l Adenine and roots on Elongated shoots in MS + 1.0 mg/l IAA media. Rooted plants were transferred in pot for acclimatization.

Keywords
Callus, In vitro, Pigeonpea, Regeneration
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