Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.59

  • SJR .349 (2022) Q2

  • Impact Factor .669 (2022)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

In vitro plant regeneration in pigeonpea  [Cajanus cajan (L.) MILL Sp.] using various explants

K. Nandha Abhijeeta, B. Madariya Rajesh
  • Email
1<p>Laboratory of Plant Tissue Culture, Department of Biotechnology, Junagadh Agricultural University, Junagadh-362 001, Gujarat, India.</p>
Cite article:- Abhijeeta Nandha K., Rajesh Madariya B. (2017). In vitro plant regeneration in pigeonpea [Cajanus cajan (L.) MILL Sp.] using various explants . Legume Research. 41(2): 226-229. doi: 10.18805/lr.v0i0.7844.

ABSTRACT

An efficient method for plant regeneration via callus formation has been developed in pigeonpea using two varieties as BDN-2 and GT-101. Three different explants viz. leaf, hypocotyl and root excised from 15-20 days old in vitro raised seedlings were cultured on basal MS medium. Greenish coloured callus were observed using various media according to explants. More shoots were found in MS + 1.0 mg/l NAA + 4.0 mg/l Adenine and roots on Elongated shoots in MS + 1.0 mg/l IAA media. Rooted plants were transferred in pot for acclimatization.

REFERENCES


  1. Aboshama, H.M.S. (2011). Somatic embryogenesis proliferation, maturation and germination in Cajanus cajan. World J. Agril. Sci., 7(1): 86-95.

  2. Anbazhagan, V.R. and Ganapathi, A. (1999). Somatic embryogenesis in cell suspension cultures of pigeonpea (Cajanus cajan). Pl. Cell Tissue Organ Cult., 56: 179-184. 

  3. Anonymous, (2011). Area and productivity of pigeonpea in India. (http://www.ncipm.org.in).

  4. Anonymous, (2012). Area, production and productivity of pigeonpea in Gujarat. (www.autherstream.com).

  5. Geetha, N., Venkatachalam, P., Prakash, V. and LakshmiSita, G. (1998). High frequency induction of multiple shoots and plant regeneration from seedling explants of pigeonpea (Cajanus cajan L.). Curr. Sci., 75: 1036-1041.

  6. George, L. and Eapen, S. (1994). Organogenesis and embryogenesis from diverse explants of pigeonpea. Pl. Cell Rep., 13: 417-420.

  7. Jain, M. and Chaturvedi, H.C. (2004). In vitro cloning of Cajanus cajan var. Bahar through prolific shoot bud differentiation in leaf segments and production of fertile plants. Indian J. Biotech., 3: 258-262. 

  8. Joshi, P.K., Rao, P.P., Gowda, C.L.L., Jones, R.B., Silim, S.N., Saxena, K.B. and Kumar, J. (2001). The world chickpea and pigeonpea economies: facts, trends, and outlook. Patancheru 502 324, Andhra Pradesh, India: International Crops Research Institute for the Semi-Arid Tropics.

  9. Krishna, G., Reddy, P.S., Ramteke, P.W., Rambabu, P., Sohrab, S.S., Rana, D. and Bhattacharya, P. (2011). In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105. Physiol. Mol. Biol. Pl., 17(4): 375-385.

  10. Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Pl., 15: 473–497.

  11. Mohan, M.L. and Krishnamurthy, K.V.(1998). Plant regeneration in pigeonpea [Cajanus cajan (L.) Millsp.] by organogenesis. Pl. Cell Rep., 17: 705-710.

  12. Prabhakaran, M., Elumalai, S., Ramganesh, S. and Prakasam, V. (2011). High frequency induction of callus from seedling explants of pigeonpea (Cajanus cajan [L] Millsp.) for genetic transformation. Curr. Bot., 2(6):15-17.

  13. Prabhakaran, M. and Elumalai, S. (2014). An efficient in vitro regeneration of shoot from cotyledon derived callus of pigeonpea [Cajanus cajan (L.) Millsp.]. Indian Streams Res. J., 3(12): 1-7.

  14. Prasad, M.G., Prasad, T.N.V.K.V. and Sundhakar, P. (2011). In vitro proliferation of shoot regeneration from embryo of Cajanus cajan L. (var. LGG-29). J. Develop. Biol. Tissue Engg., 3(5): 62-65.

  15. Sreenivasu, K., Malik, S.K., Ananda, Kumar, P. and Sharma, R.P. (1998). Plant regeneration via somatic embryogenesis in pigeonpea (Cajanus cajan L. Millsp). Pl. Cell Rep., 17: 294-297.

  16. Steel, R.G.D. and Torrie, J.H. (1960). Principle and Procedures of Statistics. McGraw Hill Book Company, Inc. New York, pp. 99-128.

  17. Varshney, R.K., Penmetsa, R.V., Dutta, S., Kulwal, P.L., Saxena, R.K., Datta, S., Sharma, T.R., Rosen, B., Carrasquilla-Garcia, N., Farmer, A.D., Dubey, A., Saxena, K.B., Gao, J., Fakrudin, B., Singh, M.N., Singh, B.P., Wanjari, K.B., Yuan, M., Srivastava, R.K., Kilian, A., Upadhyaya, H.D., Mallikarjuna, N., Town, C.D., Bruening, G.E., He, G., May, G.D., McCombie, R., Jackson, S.A., Singh, N.K. and Cook, D.R. (2010). Pigeonpea genomics initiative (PGI): an international effort to improve crop productivity of pigeonpea (Cajanus cajan L.). Mol. Breed., 26(3): 393–408.

  18. Venkatachalam, P. and Lakshmi Sita, G. (2008). Genetic transformation as a tool for improvement of Pigeon Pea, (Cajanus cajan [L.] Millsp.) Handbook of New Technologies for Genetic Improvement of Legumes, p: 125–145. 
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)