volume 26 issue 4 (december 2003) :
PLANTLET REGENERATION IN BLACKGRAM FROM CALLUS CULTURE: EFFECT OF GENOTYPE, EXPLANT AND MEDIA COMPOSITION
1Department of Genetics, Faculty of Agriculture, B.C.K.V., Mohanpur - 741 252, India
ABSTRACT
Four blackgram genotypes were cultured in two different media namely B5 and modified MS and the effect of genotype, explant and media composition were studied to develop a protocol for in Vitro plantlet regeneration. Hypocotyls and roots of 4–7 days old seedlings recorded early callusing and faster completion of callusing. Among the four genotypes, L7 did not respond favourably. To study the effect of auxin it was found that 2, 4-D supplementation was much more effective than NAA. In modified MS with MS salts and B5 vitamins callus induction was much more faster and successful than that observed in B5. However, attempt to plantlet regeneration was not successful in B5 medium which was successful in modified MS. In this experimental protocol auxin: cytokinin ratio was 1: 3 and maintenance of this ratio appeared to be very vital for successful in vitro micropropagation of blackgram.
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