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Indian Journal of Agricultural Research, volume 35 issue 3 (september 2001) : 208 - 210

SIMPLE METHODS FOR DETECTING LIGNOLYTle ENZYMES IN SOLID MEDIUM

Alka Gupta·, Murali Gopal·1, R.C.Kuhad

Affiliations

¹Dept. of Microbiology, South Campus, Delhi University, New Delhi-110 021, India

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Cite article:- Gupta· Alka, Gopal·1 Murali, R.C.Kuhad (2024). SIMPLE METHODS FOR DETECTING LIGNOLYTle ENZYMES IN SOLID MEDIUM. Indian Journal of Agricultural Research. 35(3): 208 - 210. doi: .

ABSTRACT

White rot fungi are the most capable micro-organisms to degrade lignin. Lignin degradation is not catalysed by any particular enzyme but by concerted action of a group of enzymes termed as lignolytic enzymes. A simple petriplate screening procedure in solid medium for testing extracellular production of these enzymes based on change in colour of the medium and visual detection has been proposed and evaluated.

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REFERENCES

Brodie, H.J. (1975). The Birds Nest Fungi. University of Toronto Press. Toronto, p.199.
Goldstein, I.S. (19811. Organic Chemicals from Biomass. CRC Press, Boca Raton, Florida.
Gupta, Alka (1991).M.Sc. Thesis, University of DelhLNew Delhi, p. 139.
Kirk,T.K. (1983). In: The Filamentous Fungi, Vol. 4, Fungal Technology (Smith, J.E. et aI., ed.). Edward Arnold, London, pp. 265-295.
Kuhad, R.c. and Johri, B.N. (1983). J. Microbiol. 1: 81-84.
Kuhad, R.C. and Johri, B.N. (1987). Curr. Sci. 56 : 609-611.
Kuhad. R.C. and Johri, B.N. (1991).lndian J. Microbiol. 31 :291-295.
Mehta, Vibha and Gupta, J.K. (1989). Indian J. Microbiol. 29: 267-279.
Tsao. G.T. and Chiang, L.C. (1983). In: The Filamentous Fungi, Vol. 4, Fungal Technology (Smith, J.E. et aI., ed.). Edward Arnold, London, pp. 296-326.

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SIMPLE METHODS FOR DETECTING LIGNOLYTle ENZYMES IN SOLID MEDIUM

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