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Tamil Nadu Agricultural University Coimbatore, INDIA

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ACTION OF PACLOBUTRAZOL IN ACCLIMATIZING MICROPROPAGATED CITRUS PLANf.LETS

B.N. Hazarika*, v.A. Parthasarathy, V. Nagaraju

Affiliations

¹Biotechnology Laboratory (Horticulture), ICAR Research Complex for NEH Region, Umiam - 793103 Meghalaya, India

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Cite article:- Hazarika* B.N., Parthasarathy v.A., Nagaraju V. (2025). ACTION OF PACLOBUTRAZOL IN ACCLIMATIZING MICROPROPAGATED CITRUS PLANf.LETS. Indian Journal of Agricultural Research. 36(1): 57 - 60. doi: .

ABSTRACT

A protocol for in vitro acclimatization of citrus plantlets is described which ensures up to 97% ex vitro survival of plants following their transfer to polythene bag in the green house. In vitro grown microshoots were aseptically cultured in MS and B 5 media supplemented with paclobutrazol (0.25, 0.5, 0.75, 1.0, 2.0, 3.0 and 4.0 mg/l). Paclobutrazol in the rooting medium reduced plant growth but led to reduction in wilting when plantlets were transferred to green house. Leaf number and leaf weight increased in lower concentration of paclobutrazol than its higher concentration. Addition of paclobutrazol in the growth medium increased survival of plantlets on transfer to green house.

KEYWORDS

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