volume 36 issue 1 (march 2002) : 57 - 60
ACTION OF PACLOBUTRAZOL IN ACCLIMATIZING MICROPROPAGATED CITRUS PLANf.LETS
1Biotechnology Laboratory (Horticulture),
ICAR Research Complex for NEH Region, Umiam - 793103 Meghalaya, India
ABSTRACT
A protocol for in vitro acclimatization of citrus plantlets is described which ensures up to 97% ex vitro survival of plants following their transfer to polythene bag in the green house. In vitro grown microshoots were aseptically cultured in MS and B 5 media supplemented with paclobutrazol (0.25, 0.5, 0.75, 1.0, 2.0, 3.0 and 4.0 mg/l). Paclobutrazol in the rooting medium reduced plant growth but led to reduction in wilting when plantlets were transferred to green house. Leaf number and leaf weight increased in lower concentration of paclobutrazol than its higher concentration. Addition of paclobutrazol in the growth medium increased survival of plantlets on transfer to green house.
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Indian Journal of Agricultural Research
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