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Indian Journal of Agricultural Research, volume 41 issue 1 (march 2007) : 34 - 38

ISOZYMIC FORMS OF NADP+-ISOCITRATE DEHYDROGENASE IN GERMINATING SEEDS OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER

M. Ayub Ali*, W. Joykumar Singh, L. Inaotombi Devi, S. Kunjeshwori Devi, L. Rupachandra Singh

Affiliations

¹Department of Biochemistry, Manipur University, Canchipur, Manipur, India

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Cite article:- Ali* Ayub M., Singh Joykumar W., Devi Inaotombi L., Devi Kunjeshwori S., Singh Rupachandra L. (2024). ISOZYMIC FORMS OF NADP+-ISOCITRATE DEHYDROGENASE IN GERMINATING SEEDS OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER. Indian Journal of Agricultural Research. 41(1): 34 - 38. doi: .

ABSTRACT

NADP+-dependent isocitrate dehydrogenase was extracted and purified from 48 old germinated blackgram, Vigna mungo (L.) Hepper by a procedure including successive purification steps of homogenization, heat treatment, ammonium sulphate fractionation, ion exchange chromatography on DEAE-cellulose and affinity chromatography on Blue Sepharose CL-6B. In ion-exchange chromatography, the starting enzyme activity was resolved into four different enzyme peaks (isozymic forms) corresponding to elution buffer containing 25, 50, 75 and 100 mM KCl, respectively.

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ISOZYMIC FORMS OF NADP+-ISOCITRATE DEHYDROGENASE IN GERMINATING SEEDS OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER

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