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ISOZYMIC FORMS OF NADP+-ISOCITRATE DEHYDROGENASE IN GERMINATING SEEDS OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER

Article Id: ARCC2675 | Page : 34 - 38
Citation :- ISOZYMIC FORMS OF NADP+-ISOCITRATE DEHYDROGENASE IN GERMINATING SEEDS OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER.Indian Journal of Agricultural Research.2007.(41):34 - 38
M. Ayub Ali*, W. Joykumar Singh, L. Inaotombi Devi, S. Kunjeshwori Devi and L. Rupachandra Singh
Address : Department of Biochemistry, Manipur University, Canchipur, Manipur, India

Abstract

NADP+-dependent isocitrate dehydrogenase was extracted and purified from 48 old germinated blackgram, Vigna mungo (L.) Hepper by a procedure including successive purification steps of homogenization, heat treatment, ammonium sulphate fractionation, ion exchange chromatography on DEAE-cellulose and affinity chromatography on Blue Sepharose CL-6B. In ion-exchange chromatography, the starting enzyme activity was resolved into four different enzyme peaks (isozymic forms) corresponding to elution buffer containing 25, 50, 75 and 100 mM KCl, respectively.

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