volume 41 issue 1 (march 2007) : 34 - 38
ISOZYMIC FORMS OF NADP+-ISOCITRATE DEHYDROGENASE IN GERMINATING SEEDS OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER
1Department of Biochemistry,
Manipur University, Canchipur, Manipur, India
Cite article:- Ali* Ayub M., Singh Joykumar W., Devi Inaotombi L., Devi Kunjeshwori S., Singh Rupachandra L. (2025). ISOZYMIC FORMS OF NADP+-ISOCITRATE DEHYDROGENASE IN GERMINATING SEEDS OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER. Indian Journal of Agricultural Research. 41(1): 34 - 38. doi: .
ABSTRACT
NADP+-dependent isocitrate dehydrogenase was extracted and purified from 48 old germinated blackgram, Vigna mungo (L.) Hepper by a procedure including successive purification steps of homogenization, heat treatment, ammonium sulphate fractionation, ion exchange chromatography on DEAE-cellulose and affinity chromatography on Blue Sepharose CL-6B. In ion-exchange chromatography, the starting enzyme activity was resolved into four different enzyme peaks (isozymic forms) corresponding to elution buffer containing 25, 50, 75 and 100 mM KCl, respectively.
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Indian Journal of Agricultural Research
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