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Current IssueEditorial BoardOnline First ArticlesArchive

volume 41 issue 1 (march 2007) : 34 - 38

ISOZYMIC FORMS OF NADP+-ISOCITRATE DEHYDROGENASE IN GERMINATING SEEDS OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER

M
M. Ayub Ali*
W
W. Joykumar Singh
L
L. Inaotombi Devi
S
S. Kunjeshwori Devi
L
L. Rupachandra Singh
1Department of Biochemistry, Manipur University, Canchipur, Manipur, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Ali* Ayub M., Singh Joykumar W., Devi Inaotombi L., Devi Kunjeshwori S., Singh Rupachandra L. (2025). ISOZYMIC FORMS OF NADP+-ISOCITRATE DEHYDROGENASE IN GERMINATING SEEDS OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER. Indian Journal of Agricultural Research. 41(1): 34 - 38. doi: .

ABSTRACT

NADP+-dependent isocitrate dehydrogenase was extracted and purified from 48 old germinated blackgram, Vigna mungo (L.) Hepper by a procedure including successive purification steps of homogenization, heat treatment, ammonium sulphate fractionation, ion exchange chromatography on DEAE-cellulose and affinity chromatography on Blue Sepharose CL-6B. In ion-exchange chromatography, the starting enzyme activity was resolved into four different enzyme peaks (isozymic forms) corresponding to elution buffer containing 25, 50, 75 and 100 mM KCl, respectively.

KEYWORDS

    REFERENCES

    1. Chen, R.D. et al. (1989). Planta., 178: 157-163.
    2. Chen, R.D. et al. (1988). Eur. J. Biol. Chem., 175: 565-572.
    3. Curry, R.A. and Ting, I.P. (1976). Arch. Biochem Biophys., 176: 501-509.
    4. Farrell, H.M. Jr. (1980). Biochim. Biophys. Acta., 631: 11-19.
    5. Fukunaga, N. et al. (1992). J. Biochem., 112: 849-855.
    6. Galvez, S. et al. (1994). Plant Physiol., 105: 593-600.
    7. Henson, C.A. et al. (1986). Physiol. Plant, 67: 538-544.
    8. Lowry, O.H. et al. (1951). J. Biol. Chem., 193: 265-275.
    9. Meixner-Monori, B. et al. (1986). Biochem. J., 236: 549-557.
    10. Muro-Pastor, M.I. and Florencio, F.J. (1992). Eur. J. Biochem., 203: 99.
    11. Ni, W. et al. (1987). Plant Physiol., 83: 785-788.
    12. Randall, D.D. and Givan, C.V. (1981). Plant Physiol., 68: 70-73.
    13. Rasmusson, A.G. and Moller, I.M. (1990). Plant Physiol., 94: 1012-1018.
    14. Reeves, H.C. et al. (1972). Biochim. Biophys. Acta., 258: 27-39.
    15. Satoh, Y. (1972). Plant Cell Physiol., 13: 493-503.
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    Indian Journal of Agricultural Research
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      volume 41 issue 1 (march 2007) : 34 - 38

      ISOZYMIC FORMS OF NADP+-ISOCITRATE DEHYDROGENASE IN GERMINATING SEEDS OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER

      M
      M. Ayub Ali*
      W
      W. Joykumar Singh
      L
      L. Inaotombi Devi
      S
      S. Kunjeshwori Devi
      L
      L. Rupachandra Singh
      1Department of Biochemistry, Manipur University, Canchipur, Manipur, India

      • Submitted|

      • First Online |

      • doi

      Cite article:- Ali* Ayub M., Singh Joykumar W., Devi Inaotombi L., Devi Kunjeshwori S., Singh Rupachandra L. (2025). ISOZYMIC FORMS OF NADP+-ISOCITRATE DEHYDROGENASE IN GERMINATING SEEDS OF BLACKGRAM, VIGNA MUNGO (L.) HEPPER. Indian Journal of Agricultural Research. 41(1): 34 - 38. doi: .

      ABSTRACT

      NADP+-dependent isocitrate dehydrogenase was extracted and purified from 48 old germinated blackgram, Vigna mungo (L.) Hepper by a procedure including successive purification steps of homogenization, heat treatment, ammonium sulphate fractionation, ion exchange chromatography on DEAE-cellulose and affinity chromatography on Blue Sepharose CL-6B. In ion-exchange chromatography, the starting enzyme activity was resolved into four different enzyme peaks (isozymic forms) corresponding to elution buffer containing 25, 50, 75 and 100 mM KCl, respectively.

      KEYWORDS

        REFERENCES

        1. Chen, R.D. et al. (1989). Planta., 178: 157-163.
        2. Chen, R.D. et al. (1988). Eur. J. Biol. Chem., 175: 565-572.
        3. Curry, R.A. and Ting, I.P. (1976). Arch. Biochem Biophys., 176: 501-509.
        4. Farrell, H.M. Jr. (1980). Biochim. Biophys. Acta., 631: 11-19.
        5. Fukunaga, N. et al. (1992). J. Biochem., 112: 849-855.
        6. Galvez, S. et al. (1994). Plant Physiol., 105: 593-600.
        7. Henson, C.A. et al. (1986). Physiol. Plant, 67: 538-544.
        8. Lowry, O.H. et al. (1951). J. Biol. Chem., 193: 265-275.
        9. Meixner-Monori, B. et al. (1986). Biochem. J., 236: 549-557.
        10. Muro-Pastor, M.I. and Florencio, F.J. (1992). Eur. J. Biochem., 203: 99.
        11. Ni, W. et al. (1987). Plant Physiol., 83: 785-788.
        12. Randall, D.D. and Givan, C.V. (1981). Plant Physiol., 68: 70-73.
        13. Rasmusson, A.G. and Moller, I.M. (1990). Plant Physiol., 94: 1012-1018.
        14. Reeves, H.C. et al. (1972). Biochim. Biophys. Acta., 258: 27-39.
        15. Satoh, Y. (1972). Plant Cell Physiol., 13: 493-503.
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