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EFFECT OF LEAD ON PROTEIN DISSOLUTION AND PHOSPHATE MOBILIZATION IN GERMINATING OAT [AVENA SATIVA (L.) CV. HJ-8] SEEDS

Article Id: ARCC059 | Page : 402-410
Citation :- EFFECT OF LEAD ON PROTEIN DISSOLUTION AND PHOSPHATE MOBILIZATION IN GERMINATING OAT [AVENA SATIVA (L.) CV. HJ-8] SEEDS .Indian Journal of Agricultural Research.2013.(47):402-410
Bharat Bhushan1*, Ajay Pal1 and Archna Singh2 buddingbiochemist@gmail.com
Address : College of Basic Sciences and Humanity, CCS Agricultural University, Hisar- 125 004, India

Abstract

The objective of the present research was to study the impact of lead on the initial phase of life cycle of oat seeds. To achieve the objectives, a lab scale experiment was conducted and effect on protein mobilization during seed germination was quantified. In response to various concentrations of lead ranging from 25-400 ppm, the protease activity in endosperm and embryonic axis was suppressed by 34 and 29 %, respectively while increased as the germination advances towards 12 days. The results on protein mobilization and/or utilization denoted a reduced hydrolysis of endospermic protein in the presence of lead. The pool of free amino acids in the endosperm depicted the inhibition of transport of amino acids from endosperm to developing embryonic axis. A stimulatory effect on enzyme activities was recorded in the presence of 25 ppm concentration of lead while the effect was inhibitory at higher concentrations indicating the restrictions in the release of inorganic phosphorus from phoshoesters.

Keywords

Lead Mobilization Phosphate Protease Protein.

References

  1. Bansal, P., Sharma, P. and Dhindsa, K.S. (2001). Impact of lead and cadmium on activities of hydrolytic enzymes in germinating pea seeds. Annals of Agri. Bio. Research. 6 (1): 113-122.
  2. Bernhardt, D., Trutwig, A. and Barkhold, A. (1993). Synthesis of DNA and the development of amylase and phosphatase activities in cotyledons of germinating seeds of Vaccaria pyramidata. Journal of Exp. Botany. 44: 261, 695-699.
  3. Beevers, L. (1968). Protein degradation and proteolytic activity in the cotyledons of germinating pea seeds. Phytochemistry. 7: 1837-1844.
  4. Datta, J.K., Ghanty, S., Banerjee, A. and Mondal, N.K. (2009). Impact of lead on germination physiology of certain wheat cultivars (Triticum aestivum L.). J Ecophysiol Occup Hlth. 9: 145-151
  5. Deep,V., Gupta,K. and Jain,V. (2005). Effect of lead on protein mobilization in chickpea seeds during germination. Ecol Env Cons. 12 (1) : 57-61
  6. Dube, B.K., Sinha, P., Gopal,R. and Chatterjee, C. (2003). Modulation of radish metabolism by zinc phytotoxicity. Indian Journal of Plant Physiology. 8:3, 302-306.
  7. Dubey, R.S. and Sharma, K.N. (1989). Acid and alkaline phosphatases in rice seedlings growing under salinity stress. Indian. J. Plant Physiol. 32: 217-223.
  8. Dugan, M. and Pasternakiewicz, A. (2005). Chemical pollution of the natural environment (in Polish). Zesz Nauk Pol Tow Gleb. 6 : 28-36.
  9. Firenzuoli, A.M.,Vanni, P., Ramponi, G. and Baccari, V. (1968). Changes in enzyme levels during germination of seeds of Triticum durum. Plant Physiol. 43: 260-264.
  10. Garen, A. and Levinthal, C. (1960). Fine structure, genetic and chemical study of the enzyme alkaline phosphatase of E. coli. Biochem. Biophys. Acta. 38: 470-783.
  11. Gisbert, C., Clemente, R., Navarro-Avino, J., Baixauli, C., Giner,A., Serrano, R., Walker, D.J. and Bernal, M.P. (2006). Tolerance and accumulation of heavy metals by Brassicaceae species grown in contaminated soil from Mediterranean regions of Spain. Environ Exp Bot. 56: 19-27.
  12. Hamid,N., Bukhari,N. and Jawaid, F. (2010). Physiological responses of Phaseolus vulgaris to different lead concentrations. Pak J Bot. 42 (1): 239-246.
  13. Johnson, C.B., Holloway, B.R., Smith, H. and Grierson, D. (1973). Isoenzymes of acid phosphatase in germinating peas. Planta. 115: 1-10.
  14. Lennard, B.E., Girosbon, A.D. and Greenway. (1982). Effect of P deficiency and water deficit on phosphatase activities from wheat leaves. J. Exp. Bot. 33: 682-693.
  15. Lesko, K. and Simon-Sarkadi, L. (2002). Effect of cadmium stress on amino acid and polyamine content of wheat seedlings. Periodica Polytechnica Ser Chem Eng . 46 (1-2): 65-71
  16. Lowry, G.H., Rosebrough, N.J. , Farr, A.I. and Randell , R.J. (1951). Protein measurement with Folin phenol reagent. J Biol Chem. 93: 265-275.
  17. Marbach, I. and Mayer, I.M. (1976). Respiration and utilization of storage materials in wild and cultivated pea seeds during germination. Physiol Plant. 38:126-130.
  18. Mathad, P. And Pratima, H. (2009). Copper toxicity causes oxidative stress in Brassica juncea L. seedlings. Indian J Plant Physiol. 14 (4) : 397-401.
  19. Mayer,A.M. and Poljakoff-Mayber, A. (1989). The Germination of Seeds. Pergamon Press, Oxford.
  20. Mishra, A. and Choudhuri, M. A. (1997). Differential effect of Pb2+ and Hg2+ on inhibition of germination of seeds of two rice cultivars. Indian J Plant Physiol. 2(1): 41-44.
  21. Mishra, S. and Dubey, R.S. (2008). Changes in phosphate content and phosphatase activities in rice seedlings exposed to arsenite. Braz. J. Plant Physiol. 20 : 19-28.
  22. Muccifora,S., Guerranti, R., Muzzi, C., Hope-Onyekwere, S.N. ,Pagani, R., Leoncini,R. and Bellani, L.M. (2010). Ultrastructural and biochemical investigations of protein mobilization of Mucuna pruriens (L.) DC. cotyledons and embryo axis. Protoplasma. 239: 15-21.
  23. Olivares, E. (2003). The effect of lead on phyto chemistry of Tithonia diversifolia exposed to roadside automotive pollution or grown pots of Pb-supplemented soil. Brazilian Journal of Plant Physiology. 15:3, 149-158.
  24. Pritchard, S.L., Charlton,W.L., Baker,A., Graham,A. (2002). Germination and storage reserve mobilization are regulated independently in Arabidopsis. Plant J. 31:639-647.
  25. Rajeev, G., Dube, B.K., Pratima, S., Chatterjee, C. and Sinha, P. (2003). Cobalt toxicity effects on growth and metabolism of tomato. Communications in Soil Science and Plant Analysis. 34: 5-6, 619-628.
  26. Shah, K. and Dubey, R.S. (1997). Cadmium alters the phosphate levels and suppresses activity of phosphorolytic enzymes in germinating rice seeds. Journal of Agronomy and Crop Science. 179(1): 35-45.
  27. Sharma, A.D., Thakur, M., Rana, M. and Singh, K. (2004). Effects of plant growth hormones and abiotic stresses on germination, growth and phosphatase activities in Sorghum bicolour (L.) Moench seeds. Afr. J. Biotechnol. 3 : 308-312.
  28. Stoeva, N., Berova, M. and Zlatev, Z.L. (2003). Effect of arsenic on some physiological indices in maize (Zea mays L.). J. Env Protect Eco. 4 (4) : 796-801.
  29. Subramanian, A., Sundaramoorthy, P. and Lakshmanachary, A.S., Sen,D.N. and Mohammed, S. (1990) Marvels of Seeds (eds) Jodhpur University Press, Jodhpur, p.63.
  30. Tikhaya, N.I. and Fedorovskaya, M.D. (2000). Biochemical adaptation of barley root cells to toxic substances. Biology- Bulletin of the Russian Academy of Sciences. 27 (6): 579-584.
  31. Vanaja, M., Charyulu, N.V.N. and Rao, K.V.N. (2000). Effect of cadmium on carbohydrate, nucleic acid, amino acid and phenolic content in Stigeoclonium tenuekutz. Indian J Plant Physiol. 5 : 253-256.
  32. Wang, J.W. and Kao, C.H. (2005). Effect of aluminium on endosperm reserve mobilization in germinating rice grains. Biologia Plantarum. 49 : 405-409.
  33. Xiong, Z.T. (1998). Lead uptake and effects on seed germination and plant growth in a lead hyperaccumulator Brassica pekinensis. Rupr Bull Environ Contam Toxicol. 60: 285-291.
  34. Yemm, E.W. and Cocking, E.C. (1955). The determination of amino acid with ninhydrin. Analyst. 80 : 203-213.
  35. Zeid, I.M. (2001). Responses of Phaseolus vulgaris to chromium and cobalt treatments. Biologia Plantarum. 44 (1) : 111-115.

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