Study of the Procedure for Bath Ultrasound-Assisted Extraction of Total Flavonoid from Lotus Seeds and Testing Some Biological Activities

DOI: 10.18805/IJARe.A-525    | Article Id: A-525 | Page : 563-570
Citation :- Study of the Procedure for Bath Ultrasound-Assisted Extraction of Total Flavonoid from Lotus Seeds and Testing Some Biological Activities.Indian Journal Of Agricultural Research.2020.(54):563-570
D.T. Long, H.T. Kim Hong, L.L. Thuy Tram, N.T. Quynh Trang, N.P. Thuy Tien, N.T. Ngoc Hanh
Address : Hue University of Sciences, Hue University, Vietnam.
Submitted Date : 3-01-2020
Accepted Date : 11-03-2020


In this study, we investigated impact of single factor: solvent concentration ethanol, solvent pH, ultrasound time, solvent ratio: raw material and ultrasound temperature on the total flavonoid extraction from lotus seeds. Based on survey on factors affecting extraction conditions by the ultrasound bath method, all five surveyed factors were found to have strong influences on total flavonoid extraction process. From factorial design of experimental planning using surface response methodology (RSM), optimal conditions for extraction of total flavonoid from lotus seeds were found in extraction time (98.005 min); solvent concentration ethanol (86.160 %), pH solvent ethanol (4.546), bath ultrasound temperature (22.210°C) and solvent/material ratio (8.376 (v/w)). Under above optimum conditions, total extracted flavonoids was 0.812 (mg Catechin/g extract). Experimental results showed a high compatibility with estimated RSM model. Total flavonoid content obtained in the original liquid extract was 0.797 ± 0.011 (mg Catechin/g extract). The highest antioxidant activity was found to be from ethanol extract with IC50 value (0.058 ± 0.004 (µg/mL), in comparison with IC50 of ascorbic acid, 0.002 ± 0.001 (µg/mL). Moreover, 50 % of DPPH scavenging ability of liquid and condensed extract differed from each other.


Extract Flavonoid Lotus seed Nelumbo nucifera RMS


  1. Ahmed, W., Ahmad, M., Khan R.A. and Mustaq N. (2016). Promising inhibition of krait snake’s venom acetylcholinesterase by Salix nigra and its role as anticancer, antioxidant agent. Indian Journal of Animal Research. 50: 317-323.
  2. Chang, C.C., Wen, H.M., Yang, M.H., Cherm, J.C. (2002). Estimation of Total Flavonoid Content in Propolis by Two Complemen- -tary Colorimetric Methods. Journal of Food and Drug Analysis. 10(3): 178-182.
  3. Chen, Y., Fan, G.R., Wu, H.L., Wu, Y.T. and Mitchell. (2007). ASeparation, identification and rapid determination of liensine, isoliensinine and neferine from embryo of the seed of Nelumbo nucifera Gaertn. by liquid chromatography coupled to diode array detector and tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. 43: 99-104. 
  4. Das, S., Ray, B., Ghosal, P.K. (1992). Structural studies of a polysaccharide from the seeds of Nelumbo nucifera. Carbohydrate Research. 224: 331-335.
  5. Fazekas, A.J., Burgess, K.S., Kesanakurti, P.R., Graham, S.W., Newmaster, S.G., Husband, B.C., Percy, D.M., Hajibabaei, M., Barrett, S.C. (2008). Multiple multilocus DNA barcodes from the plastid genome discriminate plant species equally well. PLoS ONE. 3: e2802.
  6. Guan, X., Yao, H. (2008). Optimization of viscozyme L assisted extraction of oat bran protein using response surface methodology. Food Chemistry. 106: 345-351.
  7. Hughes, J.P., Rees, S., Kalindjian, S.B., Philpott, K.L. (2011). Principles of early drug discovery. British Journal of Pharmacology. 162(6): 1239-1249.
  8. Itoh, A., Saitoh, T., Tani, K., Uchigaki, M., Sugimoto, Y., Yamada, J., Nakajima, H., Ohshiro, H., Sun, S., Tanahashi, T. (2011). Bisbenzylisoquinoline Alkaloids from Nelumbo nucifera. Chemical and Pharmaceutical Bulletin - J-Stage. 59: 947-951. 
  9. Jiang, X.L., Wang, E.J., Wang, L., Zhang, G.L. (2018). Flavonoid glycosides and alkaloids from the embryos of Nelumbo nucifera seeds and their antioxidant activity. Fitoterapia. 125: 184-190.
  10. Jung, H.A., Jin, S.E., Choi, R.J., Kim, D.H., Kim, Y.S., Ryu, J.H., Kim, D.W., Son, Y.K., Park, J.J., Choi, J.S. (2010). Anti-amnesic activity of neferine with antioxidant and anti- infiammatory capacities, as well as inhibition of ChEs and BACE1. Life Science. 87: 420-430.
  11. Kredy, H.M., Huang, D.H., Xie, B.J., He, H., Yang, E.N., Tian, B.Q., Xiao, D. (2010). Flavonols of lotus (Nelumbo nucifera, Gaertn.) seed epicarp and their antioxidant potential. European Food Research and Technology. 231(3): 387-394.
  12. Li, S.S., Wu, J., Chen, L.G., Du, H., Xu, Y.J., Wang, L.J., Zhang, H.J., Zheng, X.C., Wang, L.S. (2014). Biogenesis of C-    glycosyl fiavones and profiling of fiavonoid glycosides in lotus (Nelumbo nucifera). PLoS ONE. 9: e108860. 
  13. Liao, C.H. and Lin, J.Y. (2011). Lotus (Nelumbo nucifera Gaertn) plumule polysaccharide protects the spleen and liver from spontaneous infiammation in non-obese diabetic mice by modulating pro-/anti-infiammatory cytokine gene expression. Food Chemistry. 129: 245-252.
  14. Liao, C.H., Guo, S.J. and Lin, J.Y. (2011). Characterisation of the chemical composition and in vitro anti-infiammation assessment of a novel lotus (Nelumbo nucifera Gaertn) plumule polysaccharide. Food Chemistry. 125: 930-935. 
  15. Marxen, K., Heinrich, K., Lippemeier, S., Hintze, R., Ruser, A., Hansen, U.P. (2007). Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of some Microalgal Species by Linear Regression Analysis of Spectro -photometric Measurements. Sensors. 7: 2080-2095.
  16. Monks, A., Scudiero, D., Skehan, P., Shoemaker, R., Paull, K., Vistica, D., Hose, C., Langley, J., Cronise, P., Vaigro-    Wolff, A., Gray-Goodrich, M. (1991). Feasibility of a high-    flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. Journal of the National Cancer Institut. 83(11): 757-766.
  17. Moritz, C. and Cicero, C. (2014). DNA barcoding: Promise and pitfalls. PLoS Biology. 2: 1529-1531.
  18. Noorjahan, C.M. and Saranya T. (2018). Antioxidant, anticancer and molecular docking activity of tulsi plant. Agricultural Science Digest. 38: 209-212.
  19. Paran, I., Michelmore, R.W. (1993). Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theoretical and Applied Genetics. 85: 985-999. 
  20. Poornima, P., Quency, R.S. and Padma, V.V. (2013). Neferine induces reactive oxygen species mediated intrinsic pathway of apoptosis in HepG2 cells. Food Chemistry. 136: 659-667.
  21. Ruvanthika, P.N., Manikandan, S. and Lalitha, S. (2017). A comparative study on phytochemical screening of aerial parts of Nelumbo nucifera gaertn by gas chromatographic mass spectrometry. International Journal of Pharmaceutical Sciences and Sesearch. 8(5): 2320-5148.
  22. Sharma, B.R., Sharma K.G., Padmashree. Rustagi S. and Awasthi K.Y. (2017). Comparative studies on general parameters of flowers and leaves of Catharanthus alba and Catharanthus roseus. Asian Journal Of Dairy and Food Research. 36: 241-245
  23. Vijayan, K., Tsou, C.H. (2010). DNA barcoding in plants: taxonomy in a new perspective. Current Science. 99: 1530-1540.
  24. Vuong, V.Q., Sathira, H., Paul, D.R., Michael, B., Phoebe, A.P., Chistopher, J.S. (2013). Effect of extraction conditions on total phenolic compounds and antioxidant activities of Carica papaya leaf aqueous extracts. Journal of Herbal Medicine. 3(3): 104-111.
  25. Xie, Y., Zhang, Y., Zhang, L.T., Zeng, S.X., Guo, Z.B. and Zheng, B.D. (2013). Protective efects of alkaloid compounds from Nelumbinis Plumula on tert-Butyl hydroperoxide-induced oxidative stress. Molecules. 18: 10285-10300

Global Footprints