Loading...

Identification of high b-Carotene lines of maize in backcross (BC2F1) population by genotyping crtRB1 Allele specific marker

DOI: 10.18805/ijare.v0iOF.3755    | Article Id: A-4521 | Page : 434-439
Citation :- Identification of high b-Carotene lines of maize in backcross (BC2F1)population by genotyping crtRB1 Allele specific marker .Indian Journal of Agricultural Research.2016.(50):434-439

Pitambara*1 and P. Nagarajan2

mili.pitambara@gmail.com
Address :

Department of Agricultural Biotechnology, Anand Agricultural University, Anand-388 110, Gujrat, India.

Submitted Date : 29-10-2015
Accepted Date : 13-05-2016

Abstract

In order to address the global problem of Vitamin A deficiency, biofortification is considered to be a cost effective and sustainable approach.  The polymorphism in crtRB1gene is associated with b-carotene levels in maize kernels. The randomly selected 10 seeds of selected maize lines in BC2F1 population cobs when analyzed for b-carotene content through HPLC,  showed confirmation of improvement in b-carotene content of 2.37 µg/g. The crtRB1 3’TE polymorphism in BC2F1 population of the cross revealed that the progenies showed segregation distortion(SD). The present study indicated the possiblity to develop a high b-carotene inbred with genetic identity closer to that of the recurrent parent.

Keywords

Biofortification Maize Vitamin A Zea Mays b-carotene.

References

  1. Babu, R, Rojas, N.P., Gao, S, Yan, J. and Pixley, K. (2012). Validation of the effects of molecular marker polymorphisms in LcyEand CrtRB1 on provitamin. A concentrations for 26 tropical maize populations. Theor. Appl. Gene. DOI 10.1007/s00122-012-1987-3.
  2. Brunner, S, K., Fengler, Morgante, M., Tingey, S. and Rafalski, A. (2005). Evolution of DNA sequence non homologies among maize inbreds. Plant Cell, 17: 343–360. 
  3. Buckner, B., Kelson, T.L. and Robertson, D.S. (1990). Cloning of the y1 locus of maize, a gene involved in the biosynthesis of carotenoids. Plant Cell, 2: 867-876.
  4. Dellaporta, S. L., Wood, J. and Hicks, J. B. (1983). A plant DNA Mini preparation version 2. Plant Mol. Biol. Rep., 1: 19-22.
  5. Eathington, S. R., Dudley, J.W. and Rufener, G.K. (1997). Usefulness of Marker-QTL Associations in Early Generation Selection. Crop Sci., 37: 1686-1693. 
  6. Gaut, B. S. and Doebley, J.F. (1997). DNA sequence evidence for the segmental allotetraploid origin of maize USA. Proc. Natl. Acad. Sci., 94: 6809–6814.
  7. Harjes, E. C., Rocheford, R.T., Ling, B., Brutnell, P.T., Kandianis, B.C., Sowinski, G.S., Tapleton, E.A., Vallabhaneni, R., Williams, M., Wurtzel, T.E., Yan, J. and Buckler, S.E. (2008). Natural genetic variation In lycopene epsilon cyclase tapped for maize biofortification. Science, 319: 330-333. 
  8. Lu, H., Romero-Severson, J. and Bernardo, R. (2002). Chromosomal regions associated with segregation distortion in maize. Theor. Appl. Genet., 105:622–628. 
  9. Pfeiffer, W. H. and McClafferty, B. (2007). HarvestPlus:Breeding crops for better nutrition. Crop Sci., 47: S88S105.12. 
  10. Sanmiguel, P. and Bennetzen, J. (1998). Evidence that a recent increase in maize genome size was caused by the massive amplification of inter gene retrotransposons. Ann. Bot., 82: 37–44.
  11. Selvi, D. Thirusendura, Senthil, N., Yuvaraj, A., John Joel, A., Mahalingam, A., Nagarajan, P., Vellaikumar, S., Srimathi, P., Raveendran, M. and Nepolean, T. “Assessment of crtRB1 Polymorphism Associated with Increased b-Carotene Content in Maize (Zea mays L.)Seeds.” Food Biotechnology 28: 41-49.
  12. Tanksley, S. D. (1989). RFLP mapping in plant breeding: New tools for an old science. Biotechnology, 7: 257–263.
  13. Vignesh, M., FirozHossain, T. Nepolean, Supradip Saha, Agrawal, P.K., Guleria, S.K., Prasanna, B.M. and Gupta, H.S. (2012). Genetic variability for kernel b-carotene and utilization of crtRB1 3’TE gene for biofortification in maize (Zea mays L.). Indian J. Genet., 72: 189-194.
  14. Weber, E.J. (1987). Carotenoids and tocols of corn grain determined by HPLC. J. Am. Oil Chem.Soc., 64: 1129– 1134.
  15. Yan, J.B., Kandianis, C.B., Harjes, C. E., Bai, L., Kim, E.H., Yang, X.H., Skinner, D.J., Fu, Z.Y., Mitchell, S., Li, Q., Fernandez, M.G., Zaharieva, M., Babu, R., Fu, Y., Palacios, N., Li, J.S., DellaPenna, D., Brutnell, T., Buckler, E.S., Warburton, M.L. and Rocheford, T. (2010). Rare genetic variation at Zea mays crtRB1 increases ß-carotene in maize grain. Nat. Genet. 42:322–327.
     

Global Footprints