Indian Journal of Agricultural Research

  • Chief EditorV. Geethalakshmi

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Research Article

Indian Journal of Agricultural Research, volume 50 issue 5 (october 2016) : 434-439

Identification of high b-Carotene lines of maize in backcross (BC2F1) population by genotyping crtRB1 Allele specific marker

Pitambara*1, P. Nagarajan2
1<p>Department of Agricultural Biotechnology,&nbsp;Anand Agricultural University, Anand-388 110, Gujrat, India.</p>
Cite article:- Pitambara*1, Nagarajan2 P. (2016). Identification of high b-Carotene lines of maize in backcross (BC2F1)population by genotyping crtRB1 Allele specific marker . Indian Journal of Agricultural Research. 50(5): 434-439. doi: 10.18805/ijare.v0iOF.3755.

ABSTRACT

In order to address the global problem of Vitamin A deficiency, biofortification is considered to be a cost effective and sustainable approach.  The polymorphism in crtRB1gene is associated with b-carotene levels in maize kernels. The randomly selected 10 seeds of selected maize lines in BC2F1 population cobs when analyzed for b-carotene content through HPLC,  showed confirmation of improvement in b-carotene content of 2.37 µg/g. The crtRB1 3’TE polymorphism in BC2F1 population of the cross revealed that the progenies showed segregation distortion(SD). The present study indicated the possiblity to develop a high b-carotene inbred with genetic identity closer to that of the recurrent parent.

REFERENCES


  1. Babu, R, Rojas, N.P., Gao, S, Yan, J. and Pixley, K. (2012). Validation of the effects of molecular marker polymorphisms in LcyEand CrtRB1 on provitamin. A concentrations for 26 tropical maize populations. Theor. Appl. Gene. DOI 10.1007/s00122-012-1987-3.

  2. Brunner, S, K., Fengler, Morgante, M., Tingey, S. and Rafalski, A. (2005). Evolution of DNA sequence non homologies among maize inbreds. Plant Cell, 17: 343–360. 

  3. Buckner, B., Kelson, T.L. and Robertson, D.S. (1990). Cloning of the y1 locus of maize, a gene involved in the biosynthesis of carotenoids. Plant Cell, 2: 867-876.

  4. Dellaporta, S. L., Wood, J. and Hicks, J. B. (1983). A plant DNA Mini preparation version 2. Plant Mol. Biol. Rep., 1: 19-22.

  5. Eathington, S. R., Dudley, J.W. and Rufener, G.K. (1997). Usefulness of Marker-QTL Associations in Early Generation Selection. Crop Sci., 37: 1686-1693. 

  6. Gaut, B. S. and Doebley, J.F. (1997). DNA sequence evidence for the segmental allotetraploid origin of maize USA. Proc. Natl. Acad. Sci., 94: 6809–6814.

  7. Harjes, E. C., Rocheford, R.T., Ling, B., Brutnell, P.T., Kandianis, B.C., Sowinski, G.S., Tapleton, E.A., Vallabhaneni, R., Williams, M., Wurtzel, T.E., Yan, J. and Buckler, S.E. (2008). Natural genetic variation In lycopene epsilon cyclase tapped for maize biofortification. Science, 319: 330-333. 

  8. Lu, H., Romero-Severson, J. and Bernardo, R. (2002). Chromosomal regions associated with segregation distortion in maize. Theor. Appl. Genet., 105:622–628. 

  9. Pfeiffer, W. H. and McClafferty, B. (2007). HarvestPlus:Breeding crops for better nutrition. Crop Sci., 47: S88S105.12. 

  10. Sanmiguel, P. and Bennetzen, J. (1998). Evidence that a recent increase in maize genome size was caused by the massive amplification of inter gene retrotransposons. Ann. Bot., 82: 37–44.

  11. Selvi, D. Thirusendura, Senthil, N., Yuvaraj, A., John Joel, A., Mahalingam, A., Nagarajan, P., Vellaikumar, S., Srimathi, P., Raveendran, M. and Nepolean, T. “Assessment of crtRB1 Polymorphism Associated with Increased b-Carotene Content in Maize (Zea mays L.)Seeds.” Food Biotechnology 28: 41-49.

  12. Tanksley, S. D. (1989). RFLP mapping in plant breeding: New tools for an old science. Biotechnology, 7: 257–263.

  13. Vignesh, M., FirozHossain, T. Nepolean, Supradip Saha, Agrawal, P.K., Guleria, S.K., Prasanna, B.M. and Gupta, H.S. (2012). Genetic variability for kernel b-carotene and utilization of crtRB1 3’TE gene for biofortification in maize (Zea mays L.). Indian J. Genet., 72: 189-194.

  14. Weber, E.J. (1987). Carotenoids and tocols of corn grain determined by HPLC. J. Am. Oil Chem.Soc., 64: 1129– 1134.

  15. Yan, J.B., Kandianis, C.B., Harjes, C. E., Bai, L., Kim, E.H., Yang, X.H., Skinner, D.J., Fu, Z.Y., Mitchell, S., Li, Q., Fernandez, M.G., Zaharieva, M., Babu, R., Fu, Y., Palacios, N., Li, J.S., DellaPenna, D., Brutnell, T., Buckler, E.S., Warburton, M.L. and Rocheford, T. (2010). Rare genetic variation at Zea mays crtRB1 increases ß-carotene in maize grain. Nat. Genet. 42:322–327.

     
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