Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

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  • SJR 0.293

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Indian Journal of Agricultural Research, volume 50 issue 5 (october 2016) : 434-439

Identification of high b-Carotene lines of maize in backcross (BC2F1) population by genotyping crtRB1 Allele specific marker

Pitambara*1, P. Nagarajan2
1<p>Department of Agricultural Biotechnology,&nbsp;Anand Agricultural University, Anand-388 110, Gujrat, India.</p>
Cite article:- Pitambara*1, Nagarajan2 P. (2016). Identification of high b-Carotene lines of maize in backcross (BC2F1)population by genotyping crtRB1 Allele specific marker . Indian Journal of Agricultural Research. 50(5): 434-439. doi: 10.18805/ijare.v0iOF.3755.

In order to address the global problem of Vitamin A deficiency, biofortification is considered to be a cost effective and sustainable approach.  The polymorphism in crtRB1gene is associated with b-carotene levels in maize kernels. The randomly selected 10 seeds of selected maize lines in BC2F1 population cobs when analyzed for b-carotene content through HPLC,  showed confirmation of improvement in b-carotene content of 2.37 µg/g. The crtRB1 3’TE polymorphism in BC2F1 population of the cross revealed that the progenies showed segregation distortion(SD). The present study indicated the possiblity to develop a high b-carotene inbred with genetic identity closer to that of the recurrent parent.


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