PEANUT ROOT EXTRACTS AND RESVERATROL INHIBIT LIPOPOLYSACCHARIDE-INDUCED INFLAMMATION BY SUPPRESSION OF MAPKS SIGNALING PATHWAYS IN BV-2 CELLS

DOI: 10.5958/j.0976-058X.48.1.003    | Article Id: A-152 | Page : 16-22
Citation :- PEANUT ROOT EXTRACTS AND RESVERATROL INHIBIT LIPOPOLYSACCHARIDE-INDUCED INFLAMMATION BY SUPPRESSION OF MAPKS SIGNALING PATHWAYS IN BV-2 CELLS.Indian Journal Of Agricultural Research.2014.(48):16-22
Wen-Hung Lina, Heng-Hung Kuo, Li-Hsing Ho, Hui-wen Lin1 and Chien-Wei Hou1* rolis.hou@mail.ypu.edu.tw
Address : Ph. D. Program of Technology Management, Chung Hua University, Hsinchu, Taiwan

Abstract

Peanut Root (PR), in the field of agricultural waste, contains resveratrol and bear potent antioxidative activity. The effect of the PR extract and resveratrol on lipopolysaccharide (LPS) induced inflammation of BV-2 microglial cells and acute liver injury in Sprague-Dawley (SD) rats was investigated. The results showed that the PR extract and resveratrol reduced LPS-induced nitric oxide (NO), interleukin (IL)-1, IL-6, reactive oxygen species (ROS), and prostaglandin E2 (PGE2) production in BV-2 cells. The PR extract and resveratrol significantly decreased serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in LPS-treated rats. Furthermore, the water extract, with exception of the ethanol extract, of the PR extract dose-dependently inhibit LPS-induced mitogen-activated protein kinases (MAPK), and slightly inhibit cyclooxygenase-2 (COX-2) expression in BV-2 cells. Taken together, these results indicated that the protective mechanism of PR extract involved an antioxidant effect and inhibition of MAP kinases and COX-2 expressions in LPS-induced inflammation of BV-2 cells.

Keywords

ALT AST COX-2 MAPK Resveratrol ROS.

References

  1. Abbott, J.A., Medina-Bolivar, F., Martin, E.M., Engelberth, A.S., Villagarcia, H., Clausen, E.C., and Carrier, D.J. (2010). Purification of resveratrol, arachidin-1, and arachidin-3 from hairy root cultures of peanut (Arachis hypogaea) and determination of their antioxidant activity and cytotoxicity. Biotechnol Prog 26:1344–1351.
  2. Chao, J., Lee, M.S., Amagaya, S., Liao, J.W., Wu J.B., Ho, L.K., and Peng, W.H. (2009). Hepatoprotective effect of shidagonglao on acute liver injury induced by carbon tetrachloride. Am J Chin Med 37:1085–1097.
  3. Fan, G., Tang, J.J., Bhadauria, M., Nirala, S.K., Dai, F., Zhou, B., Li, Y., and Liu, Z.L. (2009). Resveratrol ameliorates carbon tetrachloride-induced acute liver injury in mice. Environ Toxicol Pharmacol 28:350–356.
  4. Farghali, H, Cerný, D, Kameníková, L., Martínek. J, Horínek, A., Kmonícková E and Zídek, Z. (2009). Resveratrol attenuates lipopolysaccharide-induced hepatitis in D-galactosamine sensitized rats: role of nitric oxide synthase 2 and heme oxygenase-1. Nitric Oxide 21:216–225.
  5. Geppert, T.D, Whitehurst, C.E., Thompson, P. and Beutler, B. (1994). Lipopolysaccharide signals activation of tumor necrosis factor biosynthesis through the ras/raf-1/MEK/MAPK pathway. Mol Med 1:93–103.
  6. Jiang, J.X., Zhang Y, Ji SH, Zhu P, and Wang ZG. (2002). Kinetics of mitogen-activated protein kinase family in lipopolysaccharide-stimulated mouse Kupffer cells and their role in cytokine production. Shock 18:336–341.
  7. King LA, Toledo AH, Rivera-Chavez FA and Toledo-Pereyra LH. 2009. Role of p38 and JNK in liver ischemia and reperfusion. J Hepatobil Pancreat Surg 16:763–770.
  8. Kishore, R, Hill, J.R, Mcmullen MR, Frenkel J, and Nagy LE. (2002). ERK1/2 and Egr-1 contribute to increased TNF- alpha production in rat Kupffer cells after chronic ethanol feeding. Am. J. Physiol. Gastrointest. Liver Physiol 282:G6–15.
  9. Kono H, Rusyn I, Yin M, Gabele E, Yamashina S, Dikalova A, Kadiiska MB, Connor HD, Mason RP, Segal BH, Bradford BU, Holland SM, and Thurman RG. (2000). NADPH oxidasederived free radicals are key oxidants in alcohol-induced liver disease. J Clin Invest 106:867–872.
  10. Lee ES, Shin MO, Yoon S, and Moon JO. (2010). Resveratrol inhibits dimethylnitrosamine-induced hepatic fibrosis in rats. Arch Pharm Res 33:925–932.
  11. Madhyastha S, Sahu SS, and Rao G. (2013). Resveratrol for prenatal-stress-induced oxidative damage in growing brain and its consequences on survival of neurons. J Basic Clin Physiol Pharmacol 19:1–10.
  12. Malhi H, and Gores GJ. (2008). Cellular and molecular mechanisms of liver injury. Gastroenterology 134:1641–1654.
  13. Mandrekar, P, and Szabo, G. (2009). Signalling pathways in alcohol-induced liver inflammation. J Hepatol 50:1258– 1266.
  14. Min L, He B, and Hui L. (2011). Mitogen-activated protein kinases in hepatocellular carcinoma development. Semin. Cancer Biol 21:10–20.
  15. Panteva, M., Korkaya, H., and Jameel, S. (2003). Hepatitis viruses and the MAPK pathway: is this a survival strategy? Virus Res 92:131–140.
  16. Poligone B and Baldwin AS. (2001). Positive and negative regulation of NF-kappaB by COX-2: roles of different prostaglandins. J Biol Chem 276:38658–38664.
  17. Roy S, Sannigrahi S, Majumdar S, Ghosh B, and Sarkar B. (2011). Resveratrol regulates antioxidant status, inhibits cytokine expression and restricts apoptosis in carbon tetrachloride induced rat hepatic injury. Oxid Med Cell Longev 1:703676.
  18. Schnabl B, Bradham CA, Bennett BL, Manning AM, Stefanovic B, and Brenner DA. (2001). TAK1/JNK and p38 have opposite effects on rat hepatic stellate cells. Hepatology 34:953–963.
  19. Shingai Y, Fujimoto A, Nakamura M, and Masuda T. (2011). Structure and function of the oxidation products of polyphenols and identification of potent lipoxygenase inhibitors from Fe-catalyzed oxidation of resveratrol. J Agric Food Chem 59:8180–8186.
  20. Sosa S, Balick MJ, Arvigo R, Esposito RG, Pizza C, Altinier G, and Tubaro A. (2002). Screening of the topical anti- inflammatory activity of some Central American plants. J Ethnopharmacol 81:211–215.
  21. Temraz S, Mukherji D, and Shamseddine A. (2013). Potential targets for colorectal cancer prevention. Int J Mol Sci 14:17279–17303.
  22. Toledo-Pereyra LH, Lopez-Neblina F, and Toledo AH. (2008). Protein kinases in organ ischemia and reperfusion. J Inves Surg 21:215–226.
  23. Yam MF, Asmawi MZ and Basir R. (2008). An investigation of the anti-inflammatory and analgesic effects of Orthosiphon stamineus leaf extract. J Med Food 11:362–368.

Global Footprints