Background: In order to reduce the use of synthetic and chemical agents in agriculture, more and more research is turning to ecological, more environmentally friendly methods. Plant aqueous extracts are products that can be a significant source of various elements, depending on the type and quality of soil on which the plant species from which the solution is prepared is grown. 
 
Methods: The aim of this study was to investigate the influence of aqueous extracts of different plant species on the yield and quality parameters of soybean seeds (Glycine max L.). Aqueous extracts of: nettle, nettle+comfrey, banana, banana peel, onion, willow and soybeans were used foliarly. The 1st foliar treatment plants was done when first flowers opened and the 2nd treatment was done when first pod reached final length. 
 
Result: The effect of aqueous extracts depends on the agroecological conditions and the analyzed traits. In 2020 the greatest effect was achieved on the free proline, SOD, Px and CAT. In 2021 the application of certain aqueous extracts had a significant effect on the yield, gerimination energy, germination percentage and vigour seed.

Application of Plant Aqueous Extracts on Yield and Quality Parameters of Soybean Seeds (Glycine max L.)

Snakebites are considered a neglected tropical disease that affects thousands of people worldwide. The available anti-venoms are associated with numerous side effects. As an alternative, researchers have extensively studied the plants in order to obtain some unusual treatment. The current study was conceded to evaluate the effects of salix nigra (Black willow) against krait snake venom acetylcholinesterase and its role as anti-cancer, anti-oxidant agent. Standard protocols of Ellman, Meyer, Gyamfi and Ruch were used for enzyme inhibition, anti-cancer and free radicals scavenging assays respectively. The methanolic extract of Salix nigra proved to possess neutralization properties against krait snake (Bungarus Sindanus) venom acetylcholinesterase. Statistical data of the results showed that Salix nigra extract inhibited the krait venom acetylcholinesterase through concentration dependent manner. Kinetic analysis using Lineweaver Burk plot revealed that Salix nigra caused competitive type of inhibition i.e. km value increased while Vmax remain constant with an increase of extract&rsquo;s concentration. The calculated IC50 value of Salix nigra was found to be 177&micro;g/ml. In DPPH free radical scavenging assay, extract showed good scavenging potential with IC50 value of 317&micro;g/ml. Similarly, using H2O2 free radicals, the % scavenging of the extract at 100, 250, 500 and 1000&micro;g/ml was 41, 54, 69 and 74% in comparison with ascorbic acid. Moreover, the extract exhibited good cytotoxic activity against brine shrimps in a dose dependent manner. The present work suggests, for the first time, that Salix nigra extract can be used not only as a potent inhibitor of snake venom acetylcholinesterase but also for the eradication of free radicals along with significant anticancer activity.

Application of Plant Aqueous Extracts on Yield and Quality Parameters of Soybean Seeds (Glycine max L.)

Promising inhibition of krait snake’s venom acetylcholinesterase by Salix nigra and its role as anticancer, antioxidant agent