<div style="text-align:justify"><strong>Background: </strong>The porcine kobuvirus (PKV) is a single-stranded, positive-sense, RNA virus of the family Picornaviridae which composed of small, non-enveloped viruses. The PKV is suspected to cause large outbreaks of diarrhoea and dehydration along with vomition in suckling piglets with a high morbidity rate. Despite of its economical importance very few studies are available on the pathology of PKV. The present study was conducted with the aim to investigate PKV in naturally infected Indian pigs.&nbsp;<br />
<strong>Methods: </strong>A total of 41 intestinal content and tissue samples from dead pigs were collected from post-mortem facility of, ICAR-IVRI during April, 2019 to July 2020. All the carcasses were subjected to detailed necropsy examination and appropriate tissue samples were collected for histopathological examination and molecular investigation. The amplification of 3D region of PKV was carried out via RT-PCR using the specific primers. The Genetic characterization of Indian isolate of the PKV was done on the basis of viral 3D gene of PKV.&nbsp;<br />
<strong>Result: </strong>A total of six out of 41 necropsied piglets were found positive for the PKV. The diarrhoea and dehydration were observed as the main clinical signs. The thickening and clouding of brain meninges, congestion of brain, pneumonia along with catarrhal and haemorrhagic enteritis was observed as common gross lesions. Microscopic lesions observed in brain were mild to moderate perivascular cuffing, congestion of meningeal and cerebral vessels, focal gliosis and neuronophagia. Mild to severe interstitial pneumonia and emphysema was noticed in lungs. Intestinal mucosa revealed severe inflammatory changes along with sloughing of villi. The genetic characterization revealed maximum identity of 91-93% with Indian isolate of PKV MH394282 and Chinese strain of PKV FJ459905. This study reported the first pathological investigation of PKV from Indian pigs and described the molecular findings in detail. Further explorative surveillance along with epidemiological studies are highly warranted to find out the real impact of the PKV on Indian pigs.</div>


<p class="ql-align-justify">​Pathological and Molecular Investigation of Porcine Kobuvirus (PKV) from Indian Pigs</p>

<div style="text-align: justify;">Genetic diversity among 30 chickpea genotypes was assessed for quality and yield attributing and traits at Regional Agricultural Research Station, Nandyal during rabi 2016-17 under both rainfed and irrigated conditions individually. The genotypes were grown in randomized block design (RBD) with three replications each. Genetic diversity study grouped 30 chickpea genotypes into five different clusters under both the tested environments. 100 seed weight followed by cooking time for soaked seeds contributed maximum towards diversity under rainfed as well as irrigated conditions. Inter cluster average D2 values stretched between 9.32 and 21.96 under rain fed condition, while it was comparatively lower and varied from 3.77 to 15.76 under irrigated condition. Cluster II under rainfed and cluster V under irrigated were identified to be the most divergent clusters. Under irrigated, genotype RVSSG- 37 from cluster II, NBeG-840, CSJK- 25, CSJK- 24, MNK- 1, NBeG-459 from cluster III, genotype NBeG-833 from cluster IV, KAK- 2, Vihar, ICCV- 15308 from cluster V and under irrigated condition, the genotype NBeG 831 of cluster II, RVSSG- 37, NBeG-459, CSJK- 25, NBeG-840, PKV-4 from cluster III, KAK- 2 from cluster IV and Vihar from cluster V were identified to be superior and were from genetically divergent clusters so could be used in hybridization programme to produce transgressive segregants.&nbsp;</div>

Genetic diversity analysis in chickpea (<em>Cicer arietinum</em> L.) grown under rainfed and irrigated conditions for quality and yield attributing traits

​Pathological and Molecular Investigation of Porcine Kobuvirus (PKV) from Indian Pigs

Genetic diversity analysis in chickpea (Cicer arietinum L.) grown under rainfed and irrigated conditions for quality and yield attributing traits

Pathological and Molecular Investigation of Porcine Kobuvirus (PKV) from Indian Pigs