<div><strong>Background:</strong> The present study aims to evaluate the therapeutic action of Sepia pharaonis ink extract in experimentally induced polycystic ovary syndrome (PCOS) rat model and perform Immunohistochemistry to confirm the findings.<br />
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<strong>Methods: </strong>The female rats were grouped into 5 consisting of six animals each. Only water was given to the control group. For inducing PCOS, Letrozole 1mg/kg.b.wt was administered to the subsequent four groups for 21 days. To the third group, an allopathic drug, clomiphene citrate 1mg/kg was given for 19 days after PCOS induction. The SPIE was subjected to lyophilisation to make a black powder and next two groups were administered orally with two different dosages of SPIE (100mg and 200mg respectively) mixed with water after PCOS induction. Vaginal smears were taken from all groups till 21 days and then treatment was started with clomiphene citrate (CC) and SPIE. After 21 days, vaginal smears were taken from all groups till ovulation was induced. The test drug effects were studied using ovary weight both right and left, body weight, hormonal levels, histopathology of ovary and Immunohistochemistry.&nbsp;<br />
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<strong>Result:</strong> PCOS group showed rapid increase in the ovarian weight when compared to control and was reduced by CC and SPIE treatment. The levels of progesterone and estradiol were found lower and testosterone levels were found higher in PCOS rats which were favourably altered. Vaginal smears coincided with the histopathology and immunohistochemistry findings. SPIE can be used as a therapeutic drug for PCOS in future, reduce infertility complaint of females and prevent early inception of diabetes mellitus.</div>


<p>Determine Cyp17a1 and Ki67 Expressions in Pcos Induced Rat Model Treated with <em>Sepia pharaonis</em> Ink Extract Proves Effective</p>


<div>A total of 26 mice were observed with vaginal septa and 10 of them progressively developed into vaginal atresia. Among these vaginal atresia mice, 6 mice eventually suffered from uterus hydrometrocolpos syndrome (HMCPS) and polycystic ovary syndrome (PCOS) and the others were cured with surgical intervention once the vaginal orifices were found closed. Imaging and pathological observation showed that vaginal atresia led to cervix&rsquo;s lipoid secretion and uterine effusion. Moreover, Serum biochemical examination and ELISA test revealed significantly dramatic drop (about 50%) in blood triglycerides (P&lt;0.01) and double increase in aromatase (P&lt;0.01) in vaginal atresia mice. These findings give gained variable insight into further studies and provide vital guide for therapy on vaginal atresia.</div>


Determine Cyp17a1 and Ki67 Expressions in Pcos Induced Rat Model Treated with Sepia pharaonis Ink Extract Proves Effective

Vaginal Septa Caused Atresia with Hydrometrocolpos Syndrome in BALB/c Mice