Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 55 issue 10 (october 2021) : 1206-1214

Determine Cyp17a1 and Ki67 Expressions in Pcos Induced Rat Model Treated with Sepia pharaonis Ink Extract Proves Effective

J. Leonoline Ebenezer, R. Gunapriya, K. Ranganathan, R. Vijayaraghavan, M. Karthik Ganesh
1Biomedical Research Unit and Laboratory Animal Centre, Saveetha Dental College and Hospital, Velappanchavadi, Chennai-600 077, Tamil Nadu, India.
Cite article:- Ebenezer Leonoline J., Gunapriya R., Ranganathan K., Vijayaraghavan R., Ganesh Karthik M. (2021). Determine Cyp17a1 and Ki67 Expressions in Pcos Induced Rat Model Treated with Sepia pharaonis Ink Extract Proves Effective. Indian Journal of Animal Research. 55(10): 1206-1214. doi: 10.18805/IJAR.B-4204.

ABSTRACT

Background: The present study aims to evaluate the therapeutic action of Sepia pharaonis ink extract in experimentally induced polycystic ovary syndrome (PCOS) rat model and perform Immunohistochemistry to confirm the findings.
Methods: The female rats were grouped into 5 consisting of six animals each. Only water was given to the control group. For inducing PCOS, Letrozole 1mg/kg.b.wt was administered to the subsequent four groups for 21 days. To the third group, an allopathic drug, clomiphene citrate 1mg/kg was given for 19 days after PCOS induction. The SPIE was subjected to lyophilisation to make a black powder and next two groups were administered orally with two different dosages of SPIE (100mg and 200mg respectively) mixed with water after PCOS induction. Vaginal smears were taken from all groups till 21 days and then treatment was started with clomiphene citrate (CC) and SPIE. After 21 days, vaginal smears were taken from all groups till ovulation was induced. The test drug effects were studied using ovary weight both right and left, body weight, hormonal levels, histopathology of ovary and Immunohistochemistry. 
Result: PCOS group showed rapid increase in the ovarian weight when compared to control and was reduced by CC and SPIE treatment. The levels of progesterone and estradiol were found lower and testosterone levels were found higher in PCOS rats which were favourably altered. Vaginal smears coincided with the histopathology and immunohistochemistry findings. SPIE can be used as a therapeutic drug for PCOS in future, reduce infertility complaint of females and prevent early inception of diabetes mellitus.

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